Influence of standardized procedures on the reliability of hip clinical assessment

Objective: This study evaluated a standardized and personalized approach to verify the effects of conditions on intrarater and interrater reliability, standard error of measurement, and minimal detectable difference for provocative tests and range-of-motion (ROM) tests used in hip pain assessment: flexion-adduction-internal rotation (FADIR), flexion-abduction-external rotation-extension (FABER), and hip internal rotation with 90° of hip flexion (hip IR). Methods: Nineteen participants (mean [± SD] age = 24 ± 2 years; 10 women and 9 men) without lower limb or back pain were recruited. Three raters evaluated each participant during 2 testing sessions, 1 day apart. Raters performed the 3 tests in 4 conditions: classic (C), controlled pressure duration (CPD), subject-specific position (SSP), and mixed (M = CPD + SSP). Results: For intrarater reliability, the CPD condition showed the highest intraclass correlation coefficients (ICCs; mean and 95% confidence interval [CI]) for hip IRROM (0.83; 95% CI, 0.53-0.94) and FADIRROM (0.75; 95% CI, 0.60-0.89). The SSP condition showed the highest ICCs for FABERheight (0.71; 95% CI, 0.42-0.87) and FABERROM (0.62; 95% CI, 0.27-0.83). Concerning interrater reliability, the classic condition presented the highest ICCs for FABER variables (height: 0.54; 95% CI, 0.28-0.76; ROM: 0.58; 95% CI, 0.32-0.79) and hip IR ROM (0.72; 95% CI, 0.51-0.87). The CPD condition showed the highest ICC for FADIRROM (0.57; 95% CI, 0.32-0.78). Conclusion: In the conditions of this study, CPD showed the highest ICCs for hip IRROM and FADIRROM, and SSP showed the highest ICCs for FABERheight and FABERROM. © 202

Quantification and reliability of hip internal rotation and the FADIR test in supine position using a smartphone application in an asymptomatic population

Objective: The purpose of this study was to quantify and report the intrarater and interrater reliability of hip internal rotation (IR) range of motion supine with the hip and knee at 90° of flexion and for the flexion-adduction-internal rotation (FADIR) test. Hip internal rotation measured in a lying supine position with the hip and knee at 90° of flexion revealed information on hip impairments. To date no simple quantification approach has been presented in this position; therefore, the FADIR test has not been quantified yet. Methods: Twenty participants (mean ± standard deviation [SD] age, 24.0 ± 2.1 years; 10 women and 10 men) without lower-limb or back pain were recruited. Three raters evaluated each participant during 2 testing sessions, 1 day apart. A built-in smartphone compass application was used to obtain the hip IR range of motion in both procedures. Results: Mean (± SD) supine IR was 51.7° (± 9.7°) and 62.6° (± 11.4°) for men and women, respectively. Concerning the FADIR test, mean values were 41.8° (± 9.64°) and 50.1° (± 8.0°) for men and women, respectively. The mean intrarater and interrater reliability coefficients were 0.80 and 0.72 for hip IR and 0.75 and 0.40 for the FADIR test. The standard error of the mean ranged from 4.8° to 8.3° (minimal detectable difference [MDD], 13.3° to 22.9°) for hip IR and from 4.6° to 10.3° (MDD, 12.8° to 28.6°) for the FADIR test. Conclusion: Overall, the smartphone compass application is adequate to quantify hip IR in a lying supine position. However, the poor to moderate interrater reliability in the FADIR test and the size of the MDD values suggest that the FADIR test should be standardized. © 202

Steepening Plasma Density Spectra in the Ionosphere: The Crucial Role Played by a Strong E-Region

Based on the Swarm 16 Hz Advanced Plasma Density data set, and using the Swarm A satellite, we apply automatic detection of spectral breaks in seven million sampled plasma density power spectra in the high-latitude F-region ionosphere. This way, we survey the presence of plasma irregularity dissipation due to an enhanced E-region conductance, caused both by solar photoionization and particle precipitation. We introduce a new quantity named the steepening slope index (SSI) which we use to estimate the occurrence rate of break-points in sampled plasma densities. We provide an interpretation of SSI in the context of solar photoionization-induced conductance enhancements of the E-region. We present a comprehensive climatology of the SSI occurrence rate, along with statistics documenting characteristic high-latitude plasma density spectra. In the absence of steepening, the typical spectral index is 2.1. When density spectra steepen, the index is typically 1.6 at large scales, and 2.7 at small scales. We discuss the impact of high-energy deeply penetrating electron precipitation in the diffuse aurora, and precipitating electrons in the aurora at large. Here, a key finding is that near the cusp, where the F-region conductance is enhanced, spectra tend not to steepen. We find that both the diffuse and discrete aurora are modulating F-region plasma irregularity dissipation through an enhancement of E-region conductance, highlighting the role played by factors other than solar zenith angle in high-latitude plasma dynamics. The influence of E-region conductance on spectral shapes indicates the need for a new discussion of how particle precipitation can structure the local winter high-latitude F-region ionosphere

Functional characterization of a melon alcohol acyl-transferase gene family involved in the biosynthesis of ester volatiles. Identification of the crucial role of a threonine residue for enzyme activity

Volatile esters, a major class of compounds contributing to the aroma of many fruit, are synthesized by alcohol acyl-transferases (AAT). We demonstrate here that, in Charentais melon (Cucumis melo var. cantalupensis), AAT are encoded by a gene family of at least four members with amino acid identity ranging from 84% (Cm-AAT1/Cm-AAT2) and 58% (Cm-AAT1/Cm-AAT3) to only 22% (Cm-AAT1/Cm-AAT4). All encoded proteins, except Cm-AAT2, were enzymatically active upon expression in yeast and show differential substrate preferences. Cm-AAT1 protein produces a wide range of short and long-chain acyl esters but has strong preference for the formation of E-2-hexenyl acetate and hexyl hexanoate. Cm-AAT3 also accepts a wide range of substrates but with very strong preference for producing benzyl acetate. Cm-AAT4 is almost exclusively devoted to the formation of acetates, with strong preference for cinnamoyl acetate. Site directed mutagenesis demonstrated that the failure of Cm-AAT2 to produce volatile esters is related to the presence of a 268-alanine residue instead of threonine as in all active AAT proteins. Mutating 268-A into 268-T of Cm-AAT2 restored enzyme activity, while mutating 268-T into 268-A abolished activity of Cm-AAT1. Activities of all three proteins measured with the prefered substrates sharply increase during fruit ripening. The expression of all Cm-AAT genes is up-regulated during ripening and inhibited in antisense ACC oxidase melons and in fruit treated with the ethylene antagonist 1-methylcyclopropene (1-MCP), indicating a positive regulation by ethylene. The data presented in this work suggest that the multiplicity of AAT genes accounts for the great diversity of esters formed in melon

Neutron to proton ratios of quasiprojectile and midrapidity emission in the 64^{64}Zn + 64^{64}Zn reaction at 45 MeV/nucleon

Simultaneous measurement of both neutrons and charged particles emitted in the reaction $^{64}$Zn + $^{64}$Zn at 45 MeV/nucleon allows comparison of the neutron to proton ratio at midrapidity with that at projectile rapidity. The evolution of N/Z in both rapidity regimes with increasing centrality is examined. For the completely re-constructed midrapidity material one finds that the neutron-to-proton ratio is above that of the overall $^{64}$Zn + $^{64}$Zn system. In contrast, the re-constructed ratio for the quasiprojectile is below that of the overall system. This difference provides the most complete evidence to date of neutron enrichment of midrapidity nuclear matter at the expense of the quasiprojectile

The cross-fertilization of jurisprudence and the principle of proportionality : process and result from a canadian perspective

Modern comparative constitutionalism traces back at least to the practice of some states in the post-World War II era of adopting democratic regimes as well as constitutionally entrenched bills of rights. It has since been fueled by the proliferation of international human rights instruments, which has increased with the end of the Cold War. Significant attention was first paid to comparative constitutional structure, but many states have now reached another stage. As notably witnessed by the works of the Council of Europe and especially those of the Venice Commission in the domain of constitutional justice, the question now is no longer about constitutionalism, including whether rights should be constitutionally protected, as much as it is about constitutional justice: how to effectively implement constitutions. Both on a regional and a global level, mutual inspiration is increasingly drawn from the case-law of peer Courts of other countries and even other continents, which gives rise to a cross-fertilisation phenomenon. One constitutional principle that emerges from, and which is still being forged by, such cross-fertilisation is the principle according to which the limitation of human rights and freedoms must be proportional to states’ objectives, that is, the principle of proportionality. More specifically, our topic is about both the historical process of jurisprudential cross-fertilisation and its functional result as far as the principle of proportionality is concerned. We speak from a Canadian perspective. The aim here is to be able to distinguish between what is common and what is distinctive about the Canadian approach

Mixed methods, materialism and the micropolitics of the research-assemblage

We assess the potential for mixing social research methods, based upon a materialist and micropolitical analysis of the research-assemblage and of what individual research techniques and methods do in practice. Applying a DeleuzoGuattarian toolkit of assemblages, affects and capacities, we document what happens when research methods and techniques interact with the events they wish to study. Micropolitically, many of these techniques and methods have unintended effects of specifying and aggregating events, with the consequently that the knowledge produced by social inquiry is invested with these specifications and aggregations. We argue that rather than abandoning these social research tools, we may use the micropolitical analysis to assess precisely how each method affects knowledge production, and engineer the research designs we use accordingly. This forms the justification for mixing methods that are highly aggregative or specifying with those that are less so, effectively rehabilitating methods that have often been rejected by social researchers, including surveys and experiments

Potentially active regions on Titan: New processing of Cassini/VIMS data

The Cassini Visual and Infrared Mapping Spectrometer (VIMS) obtained data of Titan's surface from flybys performed during the last seven years. In the 0.8-5.2 µm range, these spectro-imaging data showed that the surface consists of a multivariable geological terrain hosting complex geological processes. The data from the seven narrow methane spectral "windows" centered at 0.93, 1.08, 1.27, 1.59, 2.03, 2.8 and 5 µm provide some information on the lower atmospheric context and the surface parameters that we want to determine. Atmospheric scattering and absorption need to be clearly evaluated before we can extract the surface properties. We apply here a statistical method [1, 2] and a radiative transfer method [3, 1] on three potentially "active" regions on Titan, i.e. regions possibly subject to change over time (in brightness and/or in color etc) [4]: Tui Regio (20°S, 130°W) [5], a 1,500-km long flow-like figure, Hotei Regio (26°S, 78°W) [6], a 700-km wide volcanic-like terrain, and Sotra Facula (15°S, 42°W) [7], a 235-km in diameter area. With our method of Principal Component Analysis (PCA) we have managed to isolate specific regions of distinct and diverse chemical composition. We have tested this method on the previously studied Sinlap crater [8], delimitating compositional heterogeneous areas compatible with the published conclusions by Le Mouélic et al. (2008). Our follow-up method focuses on retrieving the surface albedo of the three areas and of the surrounding terrains with different spectral response by applying a radiative transfer (RT) code. We have used as input most of the Cassini HASI and DISR measurements, as well as new methane absorption coefficients [9], which are important to evaluate the atmospheric contribution and to allow us to better constrain the real surface alterations, by comparing the spectra of these regions. By superposing these results onto the PCA maps, we can correlate composition and morphology. As a test case, we used our RT code to verify the varying brightness of Hotei Regio reported by other investigators based on models lacking proper simulation of the atmospheric absorption [10]. Even though we have used exactly the same dataset, we did not detect any significant surface albedo variations over time; this led us to revise the definition of "active" regions: even if these regions have not visually changed over the course of the Cassini mission, the determination of the chemical composition and the correlation with the morphological structures [11] observed in these areas do not rule out that past and/or ongoing cryovolcanic processes are still a possible interpretation. [1] Solomonidou, A. et al. (2011). Potentially active regions on Titan: New processing of Cassini/VIMS data. In preparation. [2] Stephan, K. et al. (2008). Reduction of instrument-dependent noise in hyperspectral image data using the principal component analysis: Applications to Galileo NIMS data. Planetary and Space Science 56, 406-419. [3] Hirtzig, M. et al. (2011). Applications of a new methane linelist to Cassini/VIMS spectra of Titan in the 1.28-5.2 µm range . In preparation. [4] Wall, s. D. et al. (2009). Cassini RADAR images at Hotei Arcus and western Xanadu, Titan: Evidence for geologically recent cryovolcanic activity. Journal of Geophysical Research 36, L04203, [5] Barnes, J.W. et al. (2006). Cassini observations of flow-like features in western Tui Regio, Titan. Geophysical Research Letters 33, L16204. [6] Soderblom, L.A. et al. (2009). The geology of Hotei Regio, Titan: Correlation of Cassini VIMS and RADAR. Icarus 204, 610-618. [7] Lopes, R.M.C. et al. (2010). Distribution and interplay of geologic processes on Titan from Cassini radar data. Icarus 205, 540-558. [8] Le Mouélic et al. (2008). Mapping and interpretation of Sinlap crater on Titan using Cassini VIMS and RADAR data. Journal of Geophysical Research 113, E04003. [9] Campargue, A. et al. (2011). An empirical line list for methane at 80 K and 296 K in the 1.26-1.71 µm region for planetary investigations. Application to Titan. Icarus. Submitted. [10] Nelson, R. et al (2009). Saturn's Titan: Surface change, ammonia, and implications for atmospheric and tectonic activity. Icarus 199, 429-441. [11] Solomonidou, A. et al. (2011). Possible morphotectonic features on Titan and their origin. Planetary and Space Science. Submitted

Calibration of myocardial T2 and T1 against iron concentration.

BACKGROUND: The assessment of myocardial iron using T2* cardiovascular magnetic resonance (CMR) has been validated and calibrated, and is in clinical use. However, there is very limited data assessing the relaxation parameters T1 and T2 for measurement of human myocardial iron. METHODS: Twelve hearts were examined from transfusion-dependent patients: 11 with end-stage heart failure, either following death (n=7) or cardiac transplantation (n=4), and 1 heart from a patient who died from a stroke with no cardiac iron loading. Ex-vivo R1 and R2 measurements (R1=1/T1 and R2=1/T2) at 1.5 Tesla were compared with myocardial iron concentration measured using inductively coupled plasma atomic emission spectroscopy. RESULTS: From a single myocardial slice in formalin which was repeatedly examined, a modest decrease in T2 was observed with time, from mean (± SD) 23.7 ± 0.93 ms at baseline (13 days after death and formalin fixation) to 18.5 ± 1.41 ms at day 566 (p<0.001). Raw T2 values were therefore adjusted to correct for this fall over time. Myocardial R2 was correlated with iron concentration [Fe] (R2 0.566, p<0.001), but the correlation was stronger between LnR2 and Ln[Fe] (R2 0.790, p<0.001). The relation was [Fe] = 5081•(T2)-2.22 between T2 (ms) and myocardial iron (mg/g dry weight). Analysis of T1 proved challenging with a dichotomous distribution of T1, with very short T1 (mean 72.3 ± 25.8 ms) that was independent of iron concentration in all hearts stored in formalin for greater than 12 months. In the remaining hearts stored for <10 weeks prior to scanning, LnR1 and iron concentration were correlated but with marked scatter (R2 0.517, p<0.001). A linear relationship was present between T1 and T2 in the hearts stored for a short period (R2 0.657, p<0.001). CONCLUSION: Myocardial T2 correlates well with myocardial iron concentration, which raises the possibility that T2 may provide additive information to T2* for patients with myocardial siderosis. However, ex-vivo T1 measurements are less reliable due to the severe chemical effects of formalin on T1 shortening, and therefore T1 calibration may only be practical from in-vivo human studies

Dioxin Toxicity In Vivo Results from an Increase in the Dioxin-Independent Transcriptional Activity of the Aryl Hydrocarbon Receptor

The Aryl hydrocarbon receptor (Ahr) is the nuclear receptor mediating the toxicity of dioxins -widespread and persistent pollutants whose toxic effects include tumor promotion, teratogenesis, wasting syndrome and chloracne. Elimination of Ahr in mice eliminates dioxin toxicity but also produces adverse effects, some seemingly unrelated to dioxin. Thus the relationship between the toxic and dioxin-independent functions of Ahr is not clear, which hampers understanding and treatment of dioxin toxicity. Here we develop a Drosophila model to show that dioxin actually increases the in vivo dioxin-independent activity of Ahr. This hyperactivation resembles the effects caused by an increase in the amount of its dimerisation partner Ahr nuclear translocator (Arnt) and entails an increased transcriptional potency of Ahr, in addition to the previously described effect on nuclear translocation. Thus the two apparently different functions of Ahr, dioxin-mediated and dioxin-independent, are in fact two different levels (hyperactivated and basal, respectively) of a single function