Vibrating Wire for Beam Profile Scanning

The method for measurement of transverse profile (emittance) of the bunch by detecting of radiation arising scattering at of the bunch on the scanning wire is wide-spread. In this work the information about scattering bunch is proposed to measure using the oscillation frequency of the tightened scanning wire. In such way the system of radiation (or secondary particles) extraction and measurement can be removed. Dependence of oscillations frequency on beam scattering is determined by several factors, including changes of wire tension caused by transverse force of the beam, influence of beam self field. Preliminary calculations show that influence caused by wire heating will dominate. We have studied strain gauges on the basis of vibrating wire from various materials (tungsten, beryl bronze, niobium zirconium alloys). A scheme of self oscillations generation by alternating current in autogeneration circuit with automatic frequency adjustment was selected. Special method of wire fixation and elimination of transverse degrees of freedom allow to achieve relative stability better than 1E-5 during several days. For a tungsten wire with a fixed end dependence of frequency on temperature was 1E-5/K. Experimental results and estimates of wire heating of existing scanners show, that the wire heats up to a few hundred grades, which is enough for measurements

Organization of advocacy in various legal systems: comparative analysis

This article observes organization of providing legal services in different countries, such as the US, the UK, Germany, France, China and Russia. The authors describe the procedure of admitting to the legal profession and the sphere of legal activit

Legal regulation of public companies in the countries of the anglo-saxon legal system

The article provides an overview of public corporation legal regulation in the Anglo-American law. The article defines the peculiarities of legal regulation of public companies in the USA and the UK. It also identifies requirements that public companies must meet. A comparative analysis was conducted concerning the organization and the activities of public and private companie

The Value of Legal Terminology for the Romano-German Legal System Countries

The problems of terminology are relevant for any aspect of human life. In the field of lawmaking, the significance of this problem corresponds to the significance of laws in society. Thus, the primary aim of the article is to investigate the place of legal terminology in the countries of the continental system of law. To accomplish end, this survey attempts to substantiate that legal terminology in this legal system is of significant importance for the formation and application of legislation. To do so, a comparison is made with the construction of the legal system in the countries of the Anglo-Saxon legal system. Based on the result, Russian law is classified as a continental legal family. At the same time, based on an analysis of the key areas of development of economic legislation, a tendency has been revealed for the penetration of Anglo-Saxon legal institutions into the Russian legal system. The conclusion is formulated on the need to ensure the unity of legal terminology in legal texts. This is of particular importance when borrowing foreign legal institutions

Leaf Mesophyll Structure and Photosynthetic Activity in Calla palustris L. from Natural Habitats with Different Level of Technogenic Pollution

A study of leaf structure and CO2 assimilation rate was conducted on the emergent plant Calla palustris L. from natural habitats with different levels of technogenic pollution (the Sak-Elga River, upstream and downstream of the Karabash copper smelter, Chelyabinsk region, Russia). It was found that both chlorophyll a content andthe rate of CO2 assimilation decreased twofold in plants from the downstream site. No significant changes were observed in leaf mesophyll structure and the volume of aerenchyma in the leaf. It was shown that in plants from strongly contaminated site, the decrease in spongy mesophyll cell volume was compensated by the increase in their number, whereas the decrease in the number of chloroplasts per cell was accompanied by a growth in volume. It is concluded that the changes in the numerical and dimensional characteristics of mesophyll cells and chloroplasts provide for the viability of C. palustris under prolonged technogenic impact and demonstrate the plasticity of the photosynthetic apparatus. Keywords: emergent plant, heavy metals, adaptation, leaf structure, CO2 uptak

Transfer of synthetic human chromosome into human induced pluripotent stem cells for biomedical applications

Alphoid(tetO)-type human artificial chromosome (HAC) has been recently synthetized as a novel class of gene delivery vectors for induced pluripotent stem cell (iPSC)-based tissue replacement therapeutic approach. This HAC vector was designed to deliver copies of genes into patients with genetic diseases caused by the loss of a particular gene function. The alphoid(tetO)-HAC vector has been successfully transferred into murine embryonic stem cells (ESCs) and maintained stably as an independent chromosome during the proliferation and differentiation of these cells. Human ESCs and iPSCs have significant differences in culturing conditions and pluripotency state in comparison with the murine naïve-type ESCs and iPSCs. To date, transferring alphoid(tetO)-HAC vector into human iPSCs (hiPSCs) remains a challenging task. In this study, we performed the microcell-mediated chromosome transfer (MMCT) of alphoid(tetO)-HAC expressing the green fluorescent protein into newly generated hiPSCs. We used a recently modified MMCT method that employs an envelope protein of amphotropic murine leukemia virus as a targeting cell fusion agent. Our data provide evidence that a totally artificial vector, alphoid(tetO)-HAC, can be transferred and maintained in human iPSCs as an independent autonomous chromosome without affecting pluripotent properties of the cells. These data also open new perspectives for implementing alphoid(tetO)-HAC as a gene therapy tool in future biomedical applications

Adaptive morphophysiological features of neottia ovata (Orchidaceae) contributing to its natural colonization on fly ash deposits

In previous decades, some species of the Orchidaceae family have been found growing in man-made habitats. Neottia ovata is one of the most widespread orchids in Europe, however it is quite rare in Russia and is included in several regional Red Data Books. The purpose of this study was to compare the chemical composition and morphophysiological parameters of N. ovata from two forest communities of the Middle Urals, Russia: natural and transformed (fly ash dump of Verkhnetagil’skaya Thermal Power Station) for determining orchid adaptive features. The content of most of the studied metals in the underground parts (rhizome + roots) of N. ovata was considerably higher than in the leaves, which diminished the harmful effect of toxic metals on the aboveground organs. The adaptive changes in the leaf mesostructure of N. ovata such as an increase in epidermis thickness, the number of chloroplasts in the cell, and the internal assimilating surface were found for the first time. The orchids from the fly ash deposits were characterized by a higher content of chlorophyll b and carotenoids than plants from the natural forest community that evidenced the compensatory response on the decrease in chlorophyll a. The ability of N. ovata from the transformed habitat to maintain a relatively favorable water balance and stable assimilation indexes further contribute to its high viability. The study of orchid adaptive responses to unfavorable factors is necessary for their successful naturalization and introduction into a new environment. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.The reported study was partly funded by RFBR and the Government of Sverdlovsk region, project number 20-44-660011 and the Ministry of Science and Higher Education of the Russian Federation as part of state task of the Ural Federal University, FEUZ-2020-0057

Legal Regulation of Crowdfunding in Russia and Foreign Countries

The article discusses the legal regulation of crowdfunding in Russia and some foreign countries. Crowdfunding is analyzed as a category that is an integral part of e-commerce law. The analysis of the formation of this institution in foreign countries, such as the USA, Italy, Belgium. Conclusions have been formulated regarding risks that should be considered before investing in crowdfunding. The prospects of the development of crowdfunding in Russia are also considere

The Value of Legal Terminology for the Romano-German Legal System Countries

The problems of terminology are relevant for any aspect of human life. In the field of lawmaking, the significance of this problem corresponds to the significance of laws in society. Thus, the primary aim of the article is to investigate the place of legal terminology in the countries of the continental system of law. To accomplish end, this survey attempts to substantiate that legal terminology in this legal system is of significant importance for the formation and application of legislation. To do so, a comparison is made with the construction of the legal system in the countries of the Anglo-Saxon legal system. Based on the result, Russian law is classified as a continental legal family. At the same time, based on an analysis of the key areas of development of economic legislation, a tendency has been revealed for the penetration of Anglo-Saxon legal institutions into the Russian legal system. The conclusion is formulated on the need to ensure the unity of legal terminology in legal texts. This is of particular importance when borrowing foreign legal institution