853 research outputs found
GPR Monitoring of Volumetric Water Content in Soils Applied to Highway Construction and Maintenance
An overview is given on two experiments, a controlled pit study and a transportation application in subasphalt soils. Both experiments show that common-offset ground-penetrating radar (GPR) reflection data can be used to estimate θv to a high degree of accuracy. The methodology developed in these two experiments provides a technique for obtaining quick, noninvasive, accurate, and high-resolution estimates of θv
Field-Scale Estimation of Volumetric Water Content Using Ground-Penetrating Radar Ground Wave Techniques
Ground-penetrating radar (GPR) ground wave techniques were applied to estimate soil water content in the uppermost ∼10 cm of a 3 acre California vineyard several times over 1 year. We collected densely spaced GPR travel time measurements using 900 and 450 MHz antennas and analyzed these data to estimate water content. The spatial distribution of water content across the vineyard did not change significantly with time, although the absolute water content values varied seasonally and with irrigation. The GPR estimates of water content were compared to gravimetric water content, time domain reflectometry, and soil texture measurements. The comparisons of GPR-derived estimates of water content to gravimetric water content measurements showed that the GPR estimates had a root mean square error of volumetric water content of the order of 0.01. The results from this study indicate that GPR ground waves can be used to provide noninvasive, spatially dense estimates of shallow water content over large areas and in a rapid manner
Mapping the Volumetric Soil Water Content of a California Vineyard Using High-Frequency GPR Ground Wave Data
An attempt was made to establish the utility of ground-penetrating radar (GPR) as a quick and noninvasive field tool for shallow soil water content estimates as a function of space and time. Initially, detailed studies of collocated data, with electromagnetic velocity estimates from GPR data compared to gravimetric measurements of water content and to soil testure were carried out. Using the procedures developed during the detailed studies, full grids of GPR data were collected over the entire site several times. Data obtained indicate that incorporation of multiple frequency GPR grids can provide high-resolution estimates of soil water content variations as a function of depth as well as space and time
Characterization of Soil Water Content Variability and Soil Texture Using GPR Groundwave Techniques
Accurate characterization of near-surface soil water content is vital for guiding agricultural management decisions and for reducing the potential negative environmental impacts of agriculture. Characterizing the near-surface soil water content can be difficult, as this parameter is often both spatially and temporally variable, and obtaining sufficient measurements to describe the heterogeneity can be prohibitively expensive. Understanding the spatial correlation of near-surface soil water content can help optimize data acquisition and improve understanding of the processes controlling soil water content at the field scale. In this study, ground penetrating radar (GPR) methods were used to characterize the spatial correlation of water content in a three acre field as a function of sampling depth, season, vegetation, and soil texture. GPR data were acquired with 450 MHz and 900 MHz antennas, and measurements of the GPR groundwave were used to estimate soil water content at four different times. Additional water content estimates were obtained using time domain reflectometry measurements, and soil texture measurements were also acquired. Variograms were calculated for each set of measurements, and comparison of these variograms showed that the horizontal spatial correlation was greater for deeper water content measurements than for shallower measurements. Precipitation and irrigation were both shown to increase the spatial variability of water content, while shallowly-rooted vegetation decreased the variability. Comparison of the variograms of water content and soil texture showed that soil texture generally had greater small-scale spatial correlation than water content, and that the variability of water content in deeper soil layers was more closely correlated to soil texture than were shallower water content measurements. Lastly, cross-variograms of soil texture and water content were calculated, and co-kriging of water content estimates and soil texture measurements showed that geophysically-derived estimates of soil water content could be used to improve spatial estimation of soil texture
Regression of advanced melanoma upon withdrawal of immunosuppression: case series and literature review
We report two cases of stage IV malignant melanoma arising in patients treated with azathioprine for myasthenia gravis. In both cases, the melanoma metastases regressed upon withdrawal of immunosuppression. One patient remains melanoma free at 10 years, and the second patient experienced an 18-month disease free period. There is one prior case report in the medical literature to support full immune reconstitution for treatment in advanced immunosuppression-related melanoma, and one case series suggesting that transplant patients developing melanoma may benefit from a switch to sirolimus. Virtually, no data exist for the medical management of early stage melanoma in the immunosuppressed patients. We review the limited preclinical data in support of immune reconstitution and the data on immunosuppression as a risk factor for melanoma. We conclude that reduction or withdrawal of immunosuppression may be beneficial in patients with advanced stage melanoma and warrants further consideration in patients with early stage melanoma
Far infrared mapping of three Galactic star forming regions : W3(OH), S 209 & S 187
Three Galactic star forming regions associated with W3(OH), S209 and S187
have been simultaneously mapped in two trans-IRAS far infrared (FIR) bands
centered at ~ 140 and 200 micron using the TIFR 100 cm balloon borne FIR
telescope. These maps show extended FIR emission with structures. The HIRES
processed IRAS maps of these regions at 12, 25, 60 & 100 micron have also been
presented for comparison. Point-like sources have been extracted from the
longest waveband TIFR maps and searched for associations in the other five
bands. The diffuse emission from these regions have been quantified, which
turns out to be a significant fraction of the total emission. The spatial
distribution of cold dust (T < 30 K) for two of these sources (W3(OH) & S209),
has been determined reliably from the maps in TIFR bands. The dust temperature
and optical depth maps show complex morphology. In general the dust around S209
has been found to be warmer than that in W3(OH) region.Comment: Accepted for publication in Journal of Astrophysics and Astronomy (20
pages including 8 figures & 3 tables
An excess of emission in the dark cloud LDN 1111 with the Arcminute Microkelvin Imager
We present observations of the Lynds' dark nebula LDN 1111 made at microwave
frequencies between 14.6 and 17.2 GHz with the Arcminute Microkelvin Imager
(AMI). We find emission in this frequency band in excess of a thermal
free--free spectrum extrapolated from data at 1.4 GHz with matched uv-coverage.
This excess is > 15 sigma above the predicted emission. We fit the measured
spectrum using the spinning dust model of Drain & Lazarian (1998a) and find the
best fitting model parameters agree well with those derived from Scuba data for
this object by Visser et al. (2001).Comment: accepted MNRA
Subtyping sub-Saharan esophageal squamous cell carcinoma by comprehensive molecular analysis
Esophageal squamous cell carcinoma (ESCC) is endemic in regions of sub-Saharan Africa (SSA), where it is the third most common cancer. Here, we describe whole-exome tumor/normal sequencing and RNA transcriptomic analysis of 59 patients with ESCC in Malawi. We observed similar genetic aberrations as reported in Asian and North American cohorts, including mutations of TP53, CDKN2A, NFE2L2, CHEK2, NOTCH1, FAT1, and FBXW7. Analyses for nonhuman sequences did not reveal evidence for infection with HPV or other occult pathogens. Mutational signature analysis revealed common signatures associated with aging, cytidine deaminase activity (APOBEC), and a third signature of unknown origin, but signatures of inhaled tobacco use, aflatoxin and mismatch repair were notably absent. Based on RNA expression analysis, ESCC could be divided into 3 distinct subtypes, which were distinguished by their expression of cell cycle and neural transcripts. This study demonstrates discrete subtypes of ESCC in SSA, and suggests that the endemic nature of this disease reflects exposure to a carcinogen other than tobacco and oncogenic viruses
Ionized gas, molecules, and dust in Sh2-132
We analyze the various interstellar components of the HII region Sh2-132. The
main stellar source is the double binary system that includes the Wolf-Rayet
star WR153ab. We use radio continuum images at 408 and 1420 MHz, and HI 21cm
line data taken from the Canadian Galactic Plane Survey, molecular observations
of the 12CO(1-0) line at 115 GHz from the Five College Radio Astronomy
Observatory, and available mid and far IR observations obtained with the MSX
and IRAS satellites, respectively.
Sh2-132 is composed of two shells showing radio continuum counterparts at
both frequencies. The emission is thermal in nature. The estimated rms electron
density and ionized mass of the nebula are n_e = 20 cm^{-3} and M_HII = 1500
Mo. The distribution of the CO emission shows molecular gas bordering the
ionized nebula and interacting with it. The velocities of the molecular gas is
in the range --38 to --53 km/s, similar to the velocity of the ionized gas. The
emission at 8.3 mic. reveals a ring like feature of about 15' that encircles
the bright optical regions. This emission is due to the PAHs and marks the
location of photodissociation regions.
The gas distribution in the environs of Sh2-132 can be explained in a
scenario where the massive stars in the region photodissociated, ionized, and
swept-up the dense molecular material from the parental cloud through their
strong stellar winds and intense UV photon flux.Comment: 11 figures and 5 tables, accepted in MNRA
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Omics-driven identification and elimination of valerolactam catabolism in Pseudomonas putida KT2440 for increased product titer.
Pseudomonas putida is a promising bacterial chassis for metabolic engineering given its ability to metabolize a wide array of carbon sources, especially aromatic compounds derived from lignin. However, this omnivorous metabolism can also be a hindrance when it can naturally metabolize products produced from engineered pathways. Herein we show that P. putida is able to use valerolactam as a sole carbon source, as well as degrade caprolactam. Lactams represent important nylon precursors, and are produced in quantities exceeding one million tons per year (Zhang et al., 2017). To better understand this metabolism we use a combination of Random Barcode Transposon Sequencing (RB-TnSeq) and shotgun proteomics to identify the oplBA locus as the likely responsible amide hydrolase that initiates valerolactam catabolism. Deletion of the oplBA genes prevented P. putida from growing on valerolactam, prevented the degradation of valerolactam in rich media, and dramatically reduced caprolactam degradation under the same conditions. Deletion of oplBA, as well as pathways that compete for precursors L-lysine or 5-aminovalerate, increased the titer of valerolactam from undetectable after 48 h of production to ~90 mg/L. This work may serve as a template to rapidly eliminate undesirable metabolism in non-model hosts in future metabolic engineering efforts
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