State of the art in bile analysis in forensic toxicology

AbstractIn forensic toxicology, alternative matrices to blood are useful in case of limited, unavailable or unusable blood sample, suspected postmortem redistribution or long drug intake-to-sampling interval. The present article provides an update on the state of knowledge for the use of bile in forensic toxicology, through a review of the Medline literature from 1970 to May 2015. Bile physiology and technical aspects of analysis (sampling, storage, sample preparation and analytical methods) are reported, to highlight specificities and consequences from an analytical and interpretative point of view. A table summarizes cause of death and quantification in bile and blood of 133 compounds from more than 200 case reports, providing a useful tool for forensic physicians and toxicologists involved in interpreting bile analysis. Qualitative and quantitative interpretation is discussed. As bile/blood concentration ratios are high for numerous molecules or metabolites, bile is a matrix of choice for screening when blood concentrations are low or non-detectable: e.g., cases of weak exposure or long intake-to-death interval. Quantitative applications have been little investigated, but small molecules with low bile/blood concentration ratios seem to be good candidates for quantitative bile-based interpretation. Further experimental data on the mechanism and properties of biliary extraction of xenobiotics of forensic interest are required to improve quantitative interpretation

Kinematics of the Local Universe XIII. 21-cm line measurements of 452 galaxies with the Nan\c{c}ay radiotelescope, JHK Tully-Fisher relation and preliminary maps of the peculiar velocity field

This paper presents 452 new 21-cm neutral hydrogen line measurements carried out with the FORT receiver of the meridian transit Nan\c{c}ay radiotelescope (NRT) in the period April 2003 -- March 2005. This observational programme is part of a larger project aiming at collecting an exhaustive and magnitude-complete HI extragalactic catalogue for Tully-Fisher applications (the so-called KLUN project, for Kinematics of the Local Universe studies, end in 2008). The whole on-line HI archive of the NRT contains today reduced HI-profiles for ~4500 spiral galaxies of declination delta > -40° (http://klun.obs-nancay.fr). As an example of application, we use direct Tully-Fisher relation in three (JHK) bands in deriving distances to a large catalog of 3126 spiral galaxies distributed through the whole sky and sampling well the radial velocity range between 0 and 8000 km/s. Thanks to an iterative method accounting for selection bias and smoothing effects, we show as a preliminary output a detailed and original map of the velocity field in the Local Universe

Planetary Nebulae as standard candles XI. Application to Spiral Galaxies

We report the results of an [O III] lambda 5007 survey for planetary nebulae (PN) in three spiral galaxies: M101 (NGC 5457), M51 (NGC 5194/5195) and M96 (NGC 3368). By comparing on-band/off-band [O III] lambda 5007 images with images taken in H-alpha and broadband R, we identify 65, 64 and 74 PN candidates in each galaxy, respectively. From these data, an adopted M31 distance of 770 kpc, and the empirical planetary nebula luminosity function (PNLF), we derive distances to M101, M51, and M96 of 7.7 +/- 0.5, 8.4 +/- 0.6, and 9.6 +/- 0.6 Mpc. These observations demonstrate that the PNLF technique can be successfully applied to late-type galaxies, and provide an important overlap between the Population I and Population II distance scales. We also discuss some special problems associated with using the PNLF in spiral galaxies, including the effects of dust and the possible presence of [O III] bright supernova remnants.Comment: 38 pages, TeX, with tables included but not figures. Uses epsf.tex and kpnobasic.tex. To be published in the Astophysical Journal. Full paper is available at http://www.astro.psu.edu/users/johnf/Text/research.htm

Extragalactic database. VII Reduction of astrophysical parameters

The Lyon-Meudon Extragalactic database (LEDA) gives a free access to the main astrophysical parameters for more than 100,000 galaxies. The most common names are compiled allowing users to recover quickly any galaxy. All these measured astrophysical parameters are first reduced to a common system according to well defined reduction formulae leading to mean homogeneized parameters. Further, these parameters are also transformed into corrected parameters from widely accepted models. For instance, raw 21-cm line widths are transformed into mean standard widths after correction for instrumental effect and then into maximum velocity rotation properly corrected for inclination and non-circular velocity. This paper presents the reduction formulae for each parameter: coordinates, morphological type and luminosity class, diameter and axis ratio, apparent magnitude (UBV, IR, HI) and colors, maximum velocity rotation and central velocity dispersion, radial velocity, mean surface brightness, distance modulus and absolute magnitude, and group membership. For each of these parameters intermediate quantities are given: galactic extinction, inclination, K-correction etc.. All these parameters are available from direct connexion to LEDA (telnet lmc.univ-lyon1.fr, login: leda, no passwd OR http://www-obs.univ-lyon1.fr/leda ) and distributed on a standard CD-ROM (PGC-ROM 1996) by the Observatoire de Lyon via the CNRS (mail to [email protected]).Comment: 13 pages, 12 figures. The CDROM of the extragalactic database LEDA is available by mailing to: [email protected]

The solar type protostar IRAS16293-2422: new constraints on the physical structure

Context: The low mass protostar IRAS16293-2422 is a prototype Class 0 source with respect to the studies of the chemical structure during the initial phases of life of Solar type stars. Aims: In order to derive an accurate chemical structure, a precise determination of the source physical structure is required. The scope of the present work is the derivation of the structure of IRAS16293-2422. Methods: We have re-analyzed all available continuum data (single dish and interferometric, from millimeter to MIR) to derive accurate density and dust temperature profiles. Using ISO observations of water, we have also reconstructed the gas temperature profile. Results: Our analysis shows that the envelope surrounding IRAS16293-2422 is well described by the Shu "inside-out" collapsing envelope model or a single power-law density profile with index equal to 1.8. In contrast to some previous studies, our analysis does not show evidence of a large (>/- 800 AU in diameter) cavity. Conclusions: Although IRAS16293-2422 is a multiple system composed by two or three objects, our reconstruction will be useful to derive the chemical structure of the large cold envelope surrounding these objects and the warm component, treated here as a single source, from single-dish observations of molecular emission

Complex organics in IRAS 4A revisited with ALMA and PdBI: Striking contrast between two neighbouring protostellar cores

We used the Atacama Large (sub-)Millimeter Array (ALMA) and the IRAM Plateau de Bure Interferometer (PdBI) to image, with an angular resolution of 0.5$''$ (120 au) and 1$''$ (235 au), respectively, the emission from 11 different organic molecules in the protostellar binary NGC1333 IRAS 4A. We clearly disentangled A1 and A2, the two protostellar cores present. For the first time, we were able to derive the column densities and fractional abundances simultaneously for the two objects, allowing us to analyse the chemical differences between them. Molecular emission from organic molecules is concentrated exclusively in A2 even though A1 is the strongest continuum emitter. The protostellar core A2 displays typical hot corino abundances and its deconvolved size is 70 au. In contrast, the upper limits we placed on molecular abundances for A1 are extremely low, lying about one order of magnitude below prestellar values. The difference in the amount of organic molecules present in A1 and A2 ranges between one and two orders of magnitude. Our results suggest that the optical depth of dust emission at these wavelengths is unlikely to be sufficiently high to completely hide a hot corino in A1 similar in size to that in A2. Thus, the significant contrast in molecular richness found between the two sources is most probably real. We estimate that the size of a hypothetical hot corino in A1 should be less than 12 au. Our results favour a scenario in which the protostar in A2 is either more massive and/or subject to a higher accretion rate than A1, as a result of inhomogeneous fragmentation of the parental molecular clump. This naturally explains the smaller current envelope mass in A2 with respect to A1 along with its molecular richness.Comment: Accepted in Astronomy and Astrophysic

A warped disk model for M33 and the 21-cm line width in spiral galaxies

To determine the actual HI distribution and the velocity field in the outermost disk of the spiral galaxy M33, a tilted-ring model is fitted to 21-cm line data taken with the Arecibo Telescope. Since M33 is one of the main calibrators for the extragalactic distance scale derived through the Tully-Fisher relation, the outer disk warping is of interest for a correct determination and deprojection of the galaxy's line width. Even though our best model predicts small effects on the observed line width of M33, we show that similar outer disk warping in galaxies oriented differently along our line of sight could affect the widths considerably. Therefore there may be systematic effects in the determination of the rotation velocities and dynamic masses of spiral galaxies, whose exact value depends also on which method is used for measuring the galaxy's total line width.Comment: 27 pages, ps files only, ApJ in pres

Galaxy redshift surveys selected by neutral hydrogen using FAST

We discuss the possibility of performing a substantial spectroscopic galaxy redshift survey selected via the 21cm emission from neutral hydrogen using the Five-hundred metre Aperture Spherical Telescope (FAST) to be built in China. We consider issues related to the estimation of the source counts and optimizations of the survey, and discuss the constraints on cosmological models that such a survey could provide. We find that a survey taking around two years could detect ~10^7 galaxies with an average redshift of ~0.15 making the survey complementary to those already carried out at optical wavelengths. These conservative estimates have used the z=0 HI mass function and have ignored the possibility of evolution. The results could be used to constrain Gamma = (Omega_m h) to 5 per cent and the spectral index, n_s, to 7 per cent independent of cosmic microwave background data. If we also use simulated power spectra from the Planck satellite, we can constrain w to be within 5 per cent of -1.Comment: 13 pages, 15 figures, Accepted by MNRAS, minor correction

Reduction of Movement in Neurological Diseases: Effects on Neural Stem Cells Characteristics.

Both astronauts and patients affected by chronic movement-limiting pathologies face impairment in muscle and/or brain performance. Increased patient survival expectations and the expected longer stays in space by astronauts may result in prolonged motor deprivation and consequent pathological effects. Severe movement limitation can influence not only the motor and metabolic systems but also the nervous system, altering neurogenesis and the interaction between motoneurons and muscle cells. Little information is yet available about the effect of prolonged muscle disuse on neural stem cells characteristics. Our in vitro study aims to fill this gap by focusing on the biological and molecular properties of neural stem cells (NSCs). Our analysis shows that NSCs derived from the SVZ of HU mice had shown a reduced proliferation capability and an altered cell cycle. Furthermore, NSCs obtained from HU animals present an incomplete differentiation/maturation. The overall results support the existence of a link between reduction of exercise and muscle disuse and metabolism in the brain and thus represent valuable new information that could clarify how circumstances such as the absence of load and the lack of movement that occurs in people with some neurological diseases, may affect the properties of NSCs and contribute to the negative manifestations of these conditions