Comparing Allelopathic Effects of Root and Leaf Extracts of Invasive Alliaria petiolata, Lonicera maackii and Ranunculus ficaria on Germination of Three Native Woodland Plants

Author Institution: Department of Biology, Wilmington College, Wilmington, OhioInvasive plant species can exhibit allelopathic effects on native plant species. The strength of this allelopathic effect can vary with invasive species, with target species and with type of plant tissue extract. The purpose of this study was to determine the direct effects of extracts from roots or leaves of three Midwestern US invasive plants (Alliaria petiolata, Lonicera maackii and Ranunculus ficaria) on the germination success of three native target species (Anemone virginiana, Blephilia hirsuta and Elymus hystrix) in a fully factorial experiment. Leaf extract treatments overall showed more germination inhibition compared to root extract treatments. As concentration increased, effects of extracts increased. Extracts of leaves of A. petiolata had the greatest inhibition of germination across all other treatments. Effects of root and leaf extracts of each invasive species varied with each target species. While E. hystrix showed little response to extracts of roots or leaves of L. maackii and R. ficaria, B. hirsuta and A. virginiana germination were reduced by leaf extracts of these two invasive species. This study confirms the strong direct allelopathic effects of A. petiolata, though the strength of the effect varies with target species and with type of tissue used to make extracts. This study is the first to directly compare the effects of these invasive species on a suite of native, ecologically-relevant target species

Homophilic binding of PTP mu, a receptor-type protein tyrosine phosphatase, can mediate cell-cell aggregation

The receptor-like protein tyrosine phosphatase, PTPmu, displays structural similarity to cell-cell adhesion molecules of the immunoglobulin superfamily. We have investigated the ability of human PTPmu to function in such a capacity. Expression of PTPmu, with or without the PTPase domains, by recombinant baculovirus infection of Sf9 cells induced their aggregation. However, neither a chimeric form of PTPmu, containing the extracellular and transmembrane segments of the EGF receptor and the intracellular segment of PTPmu, nor the intracellular segment of PTPmu expressed as a soluble protein induced aggregation. PTPmu mediates aggregation via a homophilic mechanism, as judged by lack of incorporation of uninfected Sf9 cells into aggregates of PTPmu-expressing cells. Homophilic binding has been demonstrated between PTPmu-coated fluorescent beads (Covaspheres) and endogenously expressed PTPmu on MvLu cells. Additionally the PTPmu-coated beads specifically bound to a bacterially expressed glutathione-S-transferase fusion protein containing the extracellular segment of PTPmu (GST/PTPmu) adsorbed to petri dishes. Covaspheres coated with the GST/PTPmu fusion protein aggregated in vitro and also bound to PTPmu expressed endogenously on MvLu cells. These results suggest that the ligand for this transmembrane PTPase is another PTPmu molecule on an adjacent cell. Thus homophilic binding interactions may be an important component of the function of PTPmu in vivo

Non-Vocal Behaviors Are More Frequent During the Decisive Negotiation Phases in Barn Owl Siblings.

Animals produce vibrations or noises by means of body movements, which can play a role in communication. These behaviors enhance signal transmission or receiver attention and could be specifically used during turn-taking phases of a reciprocal exchange of signals. In the barn owl Tyto alba, nestlings vocalize one after the other to negotiate which individual will have priority access to the impending prey item to be delivered by the parents. Owlets adjust their vocalization to their own hunger level and to their siblings' vocalization, withdrawing from the contest in front of highly vocal, and hence hungry, motivated nestmates. As sibling negotiation is a multicomponent display, we examined whether body movements could also be part of the negotiation process. To this end, we analyzed whether the vocalizations of one nestling affected its nestmate's movements in three separate experiments: in natural nests, in the lab, and using a playback procedure. Nestling barn owls move in a variety of ways, such as repeated tapping of the floor with a foot, scratching the floor with claws, or flapping wings. Body movements were more frequent during the turn-taking phases of vocal interactions, when siblings emitted longer calls and at a greater rate. Once an individual monopolized vocal activity, siblings became less vocal and less active. Moreover, owlets produced more noisy body movements during the phases of vocal interactions which are crucial to prevail in negotiation. Non-vocal physical activities might reinforce vocal signals during sibling to sibling (sib-sib) interactions, or reflect owlets' arousal, in the critical period during which they vocally settle which individual will dominate the competition

Identification of p130(cas) as a substrate for the cytosolic protein tyrosine phosphatase PTP-PEST

PTP-PEST is a ubiquitously expressed, cytosolic, mammalian protein tyrosine phosphatase (PTP) which exhibits high specific activity in vitro, We have investigated the substrate specificity of PTP-PEST by a novel substrate-trapping approach in combination within vitro dephosphorylation experiments. We initially identified a prominent 130-kDa tyrosine-phosphorylated protein in pervanadate-treated HeLa cell lysates which was preferentially dephosphorylated by PTP-PEST in vitro, In order to identify this potential substrate, mutant (substrate-trapping) forms of PTP-PEST were generated which lack catalytic activity but retain the ability to bind substrates. These mutant proteins associated in stable complex-es exclusively with the same 130-kDa protein, which was identified as p130(cas) by immunoblotting. This exclusive association was observed in lysates from several cell lines and in transfected COS cells, but was not observed with other members of the PTP family, strongly suggesting that p130(cas) represents a major physiologically relevant substrate for PTP-PEST. Our studies suggest potential roles for PTP-PEST in regulation of p130(cas) function, These functions include mitogen- and cell adhesion-induced signalling events and probable roles in transformation by various oncogenes. These results provide the first demonstration of a PTP having an inherently restricted substrate a specificity in vitro and in vivo. The methods used to identify p130(cas) as a specific substrate for PTP-PEST are potentially applicable to any PTP and should therefore prove useful in determining the physiological substrates of other members of the PTP family

Measurements of integral muon intensity at large zenith angles

High-statistics data on near-horizontal muons collected with Russian-Italian coordinate detector DECOR are analyzed. Precise measurements of muon angular distributions in zenith angle interval from 60 to 90 degrees have been performed. In total, more than 20 million muons are selected. Dependences of the absolute integral muon intensity on zenith angle for several threshold energies ranging from 1.7 GeV to 7.2 GeV are derived. Results for this region of zenith angles and threshold energies have been obtained for the first time. The dependence of integral intensity on zenith angle and threshold energy is well fitted by a simple analytical formula.Comment: 4 pages, 4 figures, 1 tabl

Hybrid Decay: A Transgenerational Epigenetic Decline in Vigor and Viability Triggered in Backcross Populations of Teosinte with Maize.

In the course of generating populations of maize with teosinte chromosomal introgressions, an unusual sickly plant phenotype was noted in individuals from crosses with two teosinte accessions collected near Valle de Bravo, Mexico. The plants of these Bravo teosinte accessions appear phenotypically normal themselves and the F1 plants appear similar to typical maize × teosinte F1s. However, upon backcrossing to maize, the BC1 and subsequent generations display a number of detrimental characteristics including shorter stature, reduced seed set, and abnormal floral structures. This phenomenon is observed in all BC individuals and there is no chromosomal segment linked to the sickly plant phenotype in advanced backcross generations. Once the sickly phenotype appears in a lineage, normal plants are never again recovered by continued backcrossing to the normal maize parent. Whole-genome shotgun sequencing reveals a small number of genomic sequences, some with homology to transposable elements, that have increased in copy number in the backcross populations. Transcriptome analysis of seedlings, which do not have striking phenotypic abnormalities, identified segments of 18 maize genes that exhibit increased expression in sickly plants. A de novo assembly of transcripts present in plants exhibiting the sickly phenotype identified a set of 59 upregulated novel transcripts. These transcripts include some examples with sequence similarity to transposable elements and other sequences present in the recurrent maize parent (W22) genome as well as novel sequences not present in the W22 genome. Genome-wide profiles of gene expression, DNA methylation, and small RNAs are similar between sickly plants and normal controls, although a few upregulated transcripts and transposable elements are associated with altered small RNA or methylation profiles. This study documents hybrid incompatibility and genome instability triggered by the backcrossing of Bravo teosinte with maize. We name this phenomenon "hybrid decay" and present ideas on the mechanism that may underlie it

The transcription factor Hey and nuclear lamins specify and maintain cell identity

The inability of differentiated cells to maintain their identity is a hallmark of age-related diseases. We found that the transcription factor Hey supervises the identity of differentiated enterocytes (ECs) in the adult Drosophila midgut. Lineage tracing established that Hey-deficient ECs are unable to maintain their unique nuclear organization and identity. To supervise cell identity, Hey determines the expression of nuclear lamins, switching from a stem-cell lamin configuration to a differentiated lamin configuration. Moreover, continued Hey expression is required to conserve large-scale nuclear organization. During aging, Hey levels decline, and EC identity and gut homeostasis are impaired, including pathological reprograming and compromised gut integrity. These phenotypes are highly similar to those observed upon acute targeting of Hey or perturbation of lamin expression in ECs in young adults. Indeed, aging phenotypes were suppressed by continued expression of Hey in ECs, suggesting that a Hey-lamin network safeguards nuclear organization and differentiated cell identity

Report on the May-June 2002 Englebright Lake deep coring campaign

This report describes the May-June 2002 Englebright Lake coring project. Englebright Lake is a 14-km-long reservoir on the Yuba River of northern California, impounded by Englebright Dam, which was completed in 1940. The sediments were cored to assess the current conditions in the reservoir as part of the California Bay-Delta Authority’s Upper Yuba River Studies Program. Sediment was collected using both hydraulic-piston and rotational coring equipment mounted on a floating drilling platform. Thirty boreholes were attempted at 7 sites spaced along the longitudinal axis of the reservoir. Complete sedimentary sections were recovered from 20 boreholes at 6 sites. In total, 335 m of sediment was cored, with 86% average recovery. The core sections (each up to 1.5 m long) were processed using a standard set of laboratory techniques, including geophysical logging of physical properties, splitting, visual descriptions, digital photography, and initial subsampling. This report presents the results of these analyses in a series of stratigraphic columns. Using the observed stratigraphy as a guide, several series of subsamples were collected for various sedimentologic, geochemical, and geochronological analyses. The results of laboratory analyses of most of these subsamples will be presented in future reports and articles