Growth of Lactobacillus paracasei ATCC 334 in a cheese model system: a biochemical approach

Growth of Lactobacillus paracasei ATCC 334, in a cheese-ripening model system based upon a medium prepared from ripening Cheddar cheese extract (CCE) was evaluated. Lactobacillus paracasei ATCC 334 grows in CCE made from cheese ripened for 2 (2mCCE), 6 (6mCCE), and 8 (8mCCE) mo, to final cell densities of 5.9 × 108, 1.2 × 108, and 2.1 × 107 cfu/mL, respectively. Biochemical analysis and mass balance equations were used to determine substrate consumption patterns and products formed in 2mCCE. The products formed included formate, acetate, and d-lactate. These data allowed us to identify the pathways likely used and to initiate metabolic flux analysis. The production of volatiles during growth of Lb. paracasei ATCC 334 in 8mCCE was monitored to evaluate the metabolic pathways utilized by Lb. paracasei during the later stages of ripening Cheddar cheese. The 2 volatiles detected at high levels were ethanol and acetate. The remaining detected volatiles are present in significantly lower amounts and likely result from amino acid, pyruvate, and acetyl-coenzyme A metabolism. Carbon balance of galactose, lactose, citrate, and phosphoserine/phosphoserine-containing peptides in terms of d-lactate, acetate, and formate are in agreement with the amounts of substrates observed in 2mCCE; however, this was not the case for 6mCCE and 8mCCE, suggesting that additional energy sources are utilized during growth of Lb. paracasei ATCC 334 in these CCE. This study provides valuable information on the biochemistry and physiology of Lb. paracasei ATCC 334 in ripening cheese

Validation of a computer-adaptive test to evaluate generic health-related quality of life

<p>Abstract</p> <p>Background</p> <p>Health Related Quality of Life (HRQoL) is a relevant variable in the evaluation of health outcomes. Questionnaires based on Classical Test Theory typically require a large number of items to evaluate HRQoL. Computer Adaptive Testing (CAT) can be used to reduce tests length while maintaining and, in some cases, improving accuracy. This study aimed at validating a CAT based on Item Response Theory (IRT) for evaluation of generic HRQoL: the CAT-Health instrument.</p> <p>Methods</p> <p>Cross-sectional study of subjects aged over 18 attending Primary Care Centres for any reason. CAT-Health was administered along with the SF-12 Health Survey. Age, gender and a checklist of chronic conditions were also collected. CAT-Health was evaluated considering: 1) feasibility: completion time and test length; 2) content range coverage, Item Exposure Rate (IER) and test precision; and 3) construct validity: differences in the CAT-Health scores according to clinical variables and correlations between both questionnaires.</p> <p>Results</p> <p>396 subjects answered CAT-Health and SF-12, 67.2% females, mean age (SD) 48.6 (17.7) years. 36.9% did not report any chronic condition. Median completion time for CAT-Health was 81 seconds (IQ range = 59-118) and it increased with age (p < 0.001). The median number of items administered was 8 (IQ range = 6-10). Neither ceiling nor floor effects were found for the score. None of the items in the pool had an IER of 100% and it was over 5% for 27.1% of the items. Test Information Function (TIF) peaked between levels -1 and 0 of HRQoL. Statistically significant differences were observed in the CAT-Health scores according to the number and type of conditions.</p> <p>Conclusions</p> <p>Although domain-specific CATs exist for various areas of HRQoL, CAT-Health is one of the first IRT-based CATs designed to evaluate generic HRQoL and it has proven feasible, valid and efficient, when administered to a broad sample of individuals attending primary care settings.</p

Uso de drogas ilícitas em sete países da América Latina: perspectivas críticas de familiares e pessoas próximas

This cross-sectional multi-centre study explored how family members and friends of illicit drug users perceived protective and risk factors, treatment facilities and policies and laws regarding illicit drug use. Family members and friends of illicit drug users were recruited in 10 urban health care outpatient units in 7 Latin American countries (Brazil, Colombia, Costa Rica, Ecuador, Guatemala, Honduras and Mexico) to complete a questionnaire. The majority of the respondents chose psycho-social factors over genetic or biological explanations as causes of drug problems. Respondents felt that families and governments were responsible for preventing drug problems. Church/religious institutions were most often mentioned in the context of accessible treatment. When asked about access to treatment facilities, the majority said that there were not enough. Shame about drug use, cost, and limited treatment options were most often cited as barriers to treatment.Este estudio transversal multicéntrico exploró como los familiares y personas cercanas de usuarios de drogas ilícitas perciben los factores de protección y los de riesgo, las facilidades de tratamiento, las iniciativas de prevención y la legislación relativa a las drogas ilícitas. Los familiares y personas cercanas de los usuarios de drogas ilícitas fueron reclutados en 10 unidades urbanas de atención de salud en ambulatorios, en 7 países de América Latina (Brasil, Colombia, Costa Rica, Ecuador, Guatemala, Honduras y México) para responder a una encuesta. Con respecto a las causas de los problemas de las drogas, la mayoría de los encuestados destacó los factores psicosociales como siendo más importantes que los factores genéticos o biológicos. Los encuestados consideraron que las familias y los gobiernos son quienes tienen más responsabilidad en la prevención de los problemas de drogas. La iglesia y las instituciones religiosas fueron mencionadas con mayor frecuencia en el contexto del acceso al tratamiento. Cuando se les preguntó sobre el acceso a las facilidades de tratamiento, la mayoría manifestó que éstas no eran suficientes. Como barreras para el tratamiento, citaron entre las más frecuentes, la vergüenza por el uso de las drogas, la falta de opciones para su tratamiento y el costo del mismo.Este estudo multicêntrico corte temporal explorou a perspectiva de familiares e pessoas próximas a usuários de drogas ilícitas sobre fatores de risco e proteção, serviços de tratamento, políticas e leis relacionadas ao uso de drogas ilícitas. Os familiares e pessoas próximas a usuários de drogas ilícitas foram recrutados em dez unidades de saúde, localizadas em grandes centros urbanos de sete países da América Latina (Brasil, Colômbia, Costa Rica, Equador, Guatemala, Honduras e México), para responderem um questionário. A maioria dos participantes escolheu fatores psicossociais e não fatores genéticos ou biológicos para explicar a causa dos problemas do uso de drogas. Responderam que familiares e governantes são os principais responsáveis pela prevenção dos problemas das drogas. As igrejas e outras instituições religiosas foram mencionadas com frequência dentro do contexto de acesso ao tratamento. A maioria dos entrevistados apontou que o acesso aos serviços que oferecem tratamentos aos usuários de drogas não é suficiente. Vergonha sobre o uso de drogas, custo e opções insuficientes de tratamento foram citados com mais frequência como as principais barreiras para o tratamento.Organization of American StatesInter-American Drug Abuse Control CommissionCentre for Addiction and Mental Health (CAMH

Illicit drug use in seven Latin American countries: critical perspectives of families and familiars

This cross-sectional multi-centre study explored how family members and friends of illicit drug users perceived protective and risk factors, treatment facilities and policies and laws regarding illicit drug use. Family members and friends of illicit drug users were recruited in 10 urban health care outpatient units in 7 Latin American countries (Brazil, Colombia, Costa Rica, Ecuador, Guatemala, Honduras and Mexico) to complete a questionnaire. The majority of the respondents chose psycho-social factors over genetic or biological explanations as causes of drug problems. Respondents felt that families and governments were responsible for preventing drug problems. Church/religious institutions were most often mentioned in the context of accessible treatment. When asked about access to treatment facilities, the majority said that there were not enough. Shame about drug use, cost, and limited treatment options were most often cited as barriers to treatment.Este estudio transversal multicéntrico exploró como los familiares y personas cercanas de usuarios de drogas ilícitas perciben los factores de protección y los de riesgo, las facilidades de tratamiento, las iniciativas de prevención y la legislación relativa a las drogas ilícitas. Los familiares y personas cercanas de los usuarios de drogas ilícitas fueron reclutados en 10 unidades urbanas de atención de salud en ambulatorios, en 7 países de América Latina (Brasil, Colombia, Costa Rica, Ecuador, Guatemala, Honduras y México) para responder a una encuesta. Con respecto a las causas de los problemas de las drogas, la mayoría de los encuestados destacó los factores psicosociales como siendo más importantes que los factores genéticos o biológicos. Los encuestados consideraron que las familias y los gobiernos son quienes tienen más responsabilidad en la prevención de los problemas de drogas. La iglesia y las instituciones religiosas fueron mencionadas con mayor frecuencia en el contexto del acceso al tratamiento. Cuando se les preguntó sobre el acceso a las facilidades de tratamiento, la mayoría manifestó que éstas no eran suficientes. Como barreras para el tratamiento, citaron entre las más frecuentes, la vergüenza por el uso de las drogas, la falta de opciones para su tratamiento y el costo del mismo.Este estudo multicêntrico corte temporal explorou a perspectiva de familiares e pessoas próximas a usuários de drogas ilícitas sobre fatores de risco e proteção, serviços de tratamento, políticas e leis relacionadas ao uso de drogas ilícitas. Os familiares e pessoas próximas a usuários de drogas ilícitas foram recrutados em dez unidades de saúde, localizadas em grandes centros urbanos de sete países da América Latina (Brasil, Colômbia, Costa Rica, Equador, Guatemala, Honduras e México), para responderem um questionário. A maioria dos participantes escolheu fatores psicossociais e não fatores genéticos ou biológicos para explicar a causa dos problemas do uso de drogas. Responderam que familiares e governantes são os principais responsáveis pela prevenção dos problemas das drogas. As igrejas e outras instituições religiosas foram mencionadas com frequência dentro do contexto de acesso ao tratamento. A maioria dos entrevistados apontou que o acesso aos serviços que oferecem tratamentos aos usuários de drogas não é suficiente. Vergonha sobre o uso de drogas, custo e opções insuficientes de tratamento foram citados com mais frequência como as principais barreiras para o tratamento

Schistosome Eggs Impair Protective Th1/Th17 Immune Responses Against Salmonella Infection

Countries with a high incidence of helminth infections are characterized by high morbidity and mortality to infections with intracellular pathogens such as Salmonella. Some patients with Salmonella-Schistosoma co-infections develop a so-called "chronic septicemic salmonellosis," with prolonged fever and enlargement of the liver and spleen. These effects are most likely due to the overall immunoregulatory activities of schistosomes such as induction of Tregs, Bregs, alternatively activated macrophages, and degradation of antibodies. However, detailed underlying mechanisms are not very well investigated. Here, we show that intraperitoneal application of live Schistosoma mansoni eggs prior to infection with Salmonella Typhimurium in mice leads to an impairment of IFN-γ and IL-17 responses together with a higher bacterial load compared to Salmonella infection alone. S. mansoni eggs were found in granulomas in the visceral peritoneum attached to the colon. Immunohistological staining revealed IPSE/alpha-1, a glycoprotein secreted from live schistosome eggs, and recruited basophils around the eggs. Noteworthy, IPSE/alpha-1 is known to trigger IL-4 and IL-13 release from basophils which in turn is known to suppress Th1/Th17 responses. Therefore, our data support a mechanism of how schistosomes impair a protective immune response against Salmonella infection and increase our understanding of helminth-bacterial co-infections

Short-term regulation of peptide YY secretion by a mixed meal or peritoneal glucose-based dialysate in patients with chronic renal failure

This is a pre-copyedited, author-produced version of an article accepted for publication in "Nephrology Dialysis Trasnplantation" following peer review. The version of the record is avaliable online at Oxford Academic website.Instituto de Salud Carlos III, PI051024Instituto de Salud Carlos III, PI070413Xunta de Galicia, PS07/12Xunta de Galicia, 2006/2

Improving the Timing of Insulin Administration in Adult Acute Care Patients

Background: The correct timing of insulin administration in diabetic patients admitted to the hospital is important for the prevention of transient and serious glycemic deviations that could lead to negative patient outcomes. In November 2021, a South Florida Hospital identified an area of opportunity for quality improvement related to the process of subcutaneous insulin administration. In addition to bar code scanning, manual verification of the insulin dose by the primary nurse and another nurse was required prior to administration. Patients were experiencing delays in the timing of their insulin dose and nurses were reporting frustration with the process. Methods: The project followed the Plan-Do-Study-Act (PDSA) cycle for performance improvement. Results: The change in medication administration workflow resulted in achievement of administering insulin within 30 minutes of the scheduled time. Ninety percent of the nurses surveyed reported improvement in their workflow when giving subcutaneous insulin to their patients (n=112). Conclusion: Interdisciplinary collaboration, innovation in education of the nursing staff, monitoring adherence to the process, and sustaining engagement among stakeholders contributed to the success of this initiative, resulting in improved workflow in subcutaneous insulin administration. Keywords: Bar code medication administration, insulin, safety, quality improvemen

Highlights from the Pierre Auger Observatory

The Pierre Auger Observatory is the world's largest cosmic ray observatory. Our current exposure reaches nearly 40,000 km$^2$ str and provides us with an unprecedented quality data set. The performance and stability of the detectors and their enhancements are described. Data analyses have led to a number of major breakthroughs. Among these we discuss the energy spectrum and the searches for large-scale anisotropies. We present analyses of our X$_{max}$ data and show how it can be interpreted in terms of mass composition. We also describe some new analyses that extract mass sensitive parameters from the 100% duty cycle SD data. A coherent interpretation of all these recent results opens new directions. The consequences regarding the cosmic ray composition and the properties of UHECR sources are briefly discussed.Comment: 9 pages, 12 figures, talk given at the 33rd International Cosmic Ray Conference, Rio de Janeiro 201

Hexosamine Biosynthetic Pathway and Glycosylation Regulate Cell Migration in Melanoma Cells

The Hexosamine Biosynthetic Pathway (HBP) is a branch of glycolysis responsible for the production of a key substrate for protein glycosylation, UDP-GlcNAc. Cancer cells present altered glucose metabolism and aberrant glycosylation, pointing to alterations on HBP. Recently it was demonstrated that HBP influences many aspects of tumor biology, including the development of metastasis. In this work we characterize HBP in melanoma cells and analyze its importance to cellular processes related to the metastatic phenotype. We demonstrate that an increase in HBP flux, as well as increased O-GlcNAcylation, leads to decreased cell motility and migration in melanoma cells. In addition, inhibition of N- and O-glycosylation glycosylation reduces cell migration. High HBP flux and inhibition of N-glycosylation decrease the activity of metalloproteases 2 and 9. Our data demonstrates that modulation of HBP and different types of glycosylation impact cell migration