145 research outputs found
Improving the nutritional status of malnourished children using soybean products in Rwanda
The prevalence of malnutrition is high in Rwanda especially in Ruhango District of Southern Province (23.5% in 2009). The contribution of soybean (Glycine max L), which is an important source of high quality and inexpensive protein and oil, to improvement of nutritional status of malnourished children is unclear. Although a lot of research has been done on the production of soybean in Rwanda, research on soybean in the diet to improve the nutritional status of malnourished children has not been done. The aim of this study was to determine the effect of soybean flour and soybean milk on the nutritional status of malnourished children under the age of five. The objective was to determine the weight gain due to each treatment in comparison with the control group. This study was conducted in Ruhango District of Southern Province Rwanda. A survey was conducted where households growing and utilizing soybean were identified and anthropometric measurements were taken on 294 children to select malnourished children. Thirty malnourished children participate in the intervention which lasted three months. One cup (250ml) of soybean milk was supplemented to ten children per day. An equal number was supplemented with 25g of soybean flour in soup (250ml). The caregivers of the ten children in the control group received nutrition education together with the two treatment groups. Anthropometric measurements were taken every month and entered in WHO ANTHRO software. Data was analyzed by regression models using GenStat 14th edition and the magnitude of weight gain due to each of the treatments was predicted at 5% level of significance. A linear mixed model was used to estimate and compareweight gain among children fed on soybean milk and soybean flour supplement in comparison with the control group. Soybean products were found to affect weight gain of children (P = 0.04). The mean weight gain was 0.9 (±0.5) kg within three months of intervention. The difference in weight gain between the two treatment groups was not significant. To improve the nutritional status of malnourished children under five years further intervention is needed in terms of education and training on soybean based diet formulation.Key words: soybean products, under five, Rwand
The effects of household food practices and diseases prevalence on nutritional status of under-five children in Ruhango District, Rwanda
Malnutrition in the Southern province of Rwanda is a significant public health concern. According to the Rwanda Demographic and Health Survey (RDHS) 2010, chronic malnutrition in children under 5 years was widespread 44% children having stunting (insufficient height for age), 11 % underweight (weight for age) and 3% wasting (weight for height). Ruhango district (Southern Rwanda) had the highest malnutrition prevalence (23.5%) in 2009. This study aimed to measure the prevalence of malnutrition among Ruhango children in 2012 and to identify relationship between comorbidities occurrence, food intake and their nutrition status. A multistage sampling was done across four hierarchical levels: district, sector, cell and village, where households (N=294) with children under five years were purposively selected to form the sampling units in which the survey was conducted. The study showed that Ruhango has improved in prevention of malnutrition as compared to 2010 study. However, stunting was still a problem with those aged between 24 and 35 months being most affected. Wasting did not affect large population of the target group. Better educated household members had less malnourished kids (3%) than those who did not attend school (12%). Co-morbidities occurrence was significantly higher in malnourished children (P = 0.006). Acute Respiratory Tract Infection had the highest occurrence (52%). The children reported to have been sick had significantly lower weight than those who were normal (P<0.05). Food intake (mean dietary diversity of 25%) was not adequate for most households and only few people took protein of high quality like meat and milk. Household food practices were tested at 95% confidence interval and this affected the nutrition status of children (P = 0.02). In households with mean dietary diversity above four food groups, only 2.17 % of children were underweight as compared to 18.18% those from households with mean dietary diversity below three. Thus, interventions to eliminate malnutrition should focus on household food diversification and disease prevention.Key words: Prevalence, malnutrition, diseases, 24hr recall
Resistance of maize varieties to the maize weevil Sitophilus zeamais (Motsch.) (Coleoptera: Curculionidae)
This study aimed at evaluating commonly used maize varieties, collected from Melkasa and Bako Agricultural Research Centers and Haramaya University, Ethiopia, against the maize weevil Sitophilus zeamais Motsch., one of the most important cosmopolitan stored product pests in maize. A total of 13 improved maize varieties were screened for their relative susceptibility to S. zeamais. The Dobie index of susceptibility was used to group the varieties. The variety, âBHQP-542â, had the least index ofsusceptibility and was regarded as resistant. The varieties, âKatumaniâ, âMelkasa-Iâ, âMelkasa-IIâ, âMelkasa-IIIâ, âCoreeâ, âBH-541â, âBH-660â, âBH-540â, âRare-Iâ, âAwasa-511â, âACV3â and âACV6â, were moderately resistant. Weevils fed with the resistant variety produced low numbers of F1 progeny, had a high median developmental time and a low percentage of seed damage and seed weight loss. Maize varieties with a high F1 progeny tended to have a short median developmental time. An increasingnumber of F1 progeny resulted in an increasing seed damage and seed weight loss. We found an inverse relationship between the susceptibility index and percent mortality and median developmental time; however, the numbers of F1 progeny, percent seed damage and seed weight loss were positively related with the susceptibility index. The use of resistant varieties should be promoted in managing S.zeamais in stored maize under subsistence farming conditions in Africa
Increased epithelial thickness and reduced HIV receptor expression in the ectocervical mucosa is associated with relative HIV resistance
Multi-basin depositional framework for moisture-balance reconstruction during the last 1300Â years at Lake Bogoria, central Kenya Rift Valley
Multi-proxy analysis of sediment cores from five key locations in hypersaline, alkaline Lake Bogoria (central Kenya Rift Valley) has allowed reconstruction of its history of depositional and hydrological change during the past 1300years. Analyses including organic matter and carbonate content, granulometry, mineralogical composition, charcoal counting and high-resolution scanning of magnetic susceptibility and elemental geochemistry resulted in a detailed sedimentological and compositional characterization of lacustrine deposits in the three lake basins and on the two sills separating them. Thesepalaeolimnological data were supplemented with information on present-day sedimentation conditions based on seasonal sampling of settling particles and on measurement of physicochemical profiles through the water column. A new age model based on Pb-210, Cs-137 and C-14 dating captures the sediment chronology of this hydrochemically complex and geothermally fed lake. An extensive set of chronological tie points between the equivalent high-resolution proxy time series of the five sediment sequences allowed transfer of radiometric dates between the basins, enabling interbasin comparison of sedimentation dynamics through time. The resulting reconstruction demonstrates considerable moisture-balance variability through time, reflecting regional hydroclimate dynamics over the past 1300years. Between ca 690 and 950AD, the central and southern basins of Lake Bogoria were reduced to shallow and separated brine pools. In the former, occasional near-complete desiccation triggered massive trona precipitation. Between ca 950 and 1100AD, slightly higher water levels allowed the build-up of high pCO(2) leading to precipitation of nahcolite still under strongly evaporative conditions. Lake Bogoria experienced a pronounced highstand between ca 1100 and 1350AD, only to recede again afterwards. For a substantial part of the time between ca 1350 and 1800AD, the northern basin was probably disconnected from the united central and southern basins. Throughout the last two centuries, lake level has been relatively high compared to the rest of the past millennium. Evidence for increased terrestrial sediment supply in recent decades, due to anthropogenic soil erosion in the wider Bogoria catchment, is a reason for concern about possible adverse impacts on the unique ecosystem of Lake Bogoria
Clinical Characteristics Associated with Mycoplasma genitalium among Female Sex Workers in Nairobi, Kenya
The prevalence of Mycoplasma genitalium is high in vulnerable populations of women in low-resource settings. However, the epidemiology of infection in these populations is not well established. To determine the prevalence of Mycoplasma genitalium and its association with cervical cytology and other correlates, we recruited 350 female sex workers (FSW) who were 18 to 50 years old in Nairobi, Kenya, for a cross-sectional study. A questionnaire was administered at baseline to obtain information on sociodemographics and sexual behaviors. Women underwent a pelvic exam, during which a physician collected cervical-exfoliation samples for conventional cytology and sexually transmitted infection (STI) testing. Samples were tested for M. genitalium and other STI organisms (Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis) and the E6/E7 mRNA of human papillomavirus (HPV) by Aptima nucleic amplification assays. The prevalence of M. genitalium was 12.9%. FSW who engaged in sexual intercourse during menses were less likely to have M. genitalium infection than those who did not (odds ratio [OR], 0.3; 95% confidence interval [95% CI], 0.1, 0.9). M. genitalium was also less prevalent among FSW who had worked in prostitution for >5 years (6.2%) than among those who had worked for <3 years (17.6%) (OR, 0.3; 95% CI, 0.1, 0.8). FSW who reported more frequent condom use were more likely to be infected with M. genitalium than those who reported less frequent use (OR, 3.8; 95% CI, 1.2, 11.6). These correlates differ from those found in M. genitalium studies conducted with FSW from West Africa and China. Further longitudinal analyses assessing associations with persistent M. genitalium infection are needed
Low HIV incidence in pregnant and postpartum women receiving a community-based combination HIV prevention intervention in a high HIV incidence setting in South Africa
BACKGROUND:
Young Southern African women have the highest HIV incidence globally. Pregnancy doubles
the risk of HIV acquisition further, and maternal HIV acquisition contributes significantly
to the paediatric HIV burden. Little data on combination HIV prevention interventions during
pregnancy and lactation are available. We measured HIV incidence amongst pregnant and
postpartum women receiving a community-based combination HIV prevention intervention
in a high HIV incidence setting in South Africa.
METHODS:
A cohort study that included HIV-uninfected pregnant women was performed. Lay community-
based workers provided individualized HIV prevention counselling and performed
three-monthly home and clinic-based individual and couples HIV testing. Male partners
were referred for circumcision, sexually transmitted infections or HIV treatment as appropriate.
Kaplan-Meier analyses and Cox's regression were used to estimate HIV incidence and
factors associated with HIV acquisition. RESULTS
The 1356 women included (median age 22.5 years) received 5289 HIV tests. Eleven new
HIV infections were detected over 828.3 person-years (PY) of follow-up, with an HIV incidence
rate of 1.33 infections/100 PY (95% CI: 0.74±2.40). Antenatally, the HIV incidence
rate was 1.49 infections/100 PY (95% CI: 0.64±2.93) and postnatally the HIV incidence rate
was 1.03 infections/100 PY (95% CI: 0.33±3.19). 53% of male partners received HIV testing
and 66% of eligible partners received referral for circumcision. Women within known serodiscordant
couples, and women with newly diagnosed HIV-infected partners, adjusted hazard
ratio (aHR) = 32.7 (95% CI: 3.8±282.2) and aHR = 126.4 (95% CI: 33.8±472.2) had
substantially increased HIV acquisition, respectively. Women with circumcised partners had
a reduced risk of incident HIV infection, aHR = 0.22 (95% CI: 0.03±1.86).
CONCLUSIONS:
Maternal HIV incidence was substantially lower than previous regional studies. Community-based
combination HIV prevention interventions may reduce high maternal HIV incidence in
resource-poor settings. Expanded roll-out of home-based couples HIV testing and initiating
pre-exposure prophylaxis for pregnant women within serodiscordant couples is needed in
Southern Africa
Regional Differences in Prevalence of HIV-1 Discordance in Africa and Enrollment of HIV-1 Discordant Couples into an HIV-1 Prevention Trial
Background: Most HIV-1 transmission in Africa occurs among HIV-1-discordant couples (one partner HIV-1 infected and one uninfected) who are unaware of their discordant HIV-1 serostatus. Given the high HIV-1 incidence among HIV-1 discordant couples and to assess efficacy of interventions for reducing HIV-1 transmission, HIV-1 discordant couples represent a critical target population for HIV-1 prevention interventions and prevention trials. Substantial regional differences exist in HIV-1 prevalence in Africa, but regional differences in HIV-1 discordance among African couples, has not previously been reported. Methodology/Principal Findings: The Partners in Prevention HSV-2/HIV-1 Transmission Trial (âPartners HSV-2 Studyâ), the first large HIV-1 prevention trial in Africa involving HIV-1 discordant couples, completed enrollment in May 2007. Partners HSV-2 Study recruitment data from 12 sites from East and Southern Africa were used to assess HIV-1 discordance among couples accessing couples HIV-1 counseling and testing, and to correlate with enrollment of HIV-1 discordant couples. HIV-1 discordance at Partners HSV-2 Study sites ranged from 8â31% of couples tested from the community. Across all study sites and, among all couples with one HIV-1 infected partner, almost half (49%) of couples were HIV-1 discordant. Site-specific monthly enrollment of HIV-1 discordant couples into the clinical trial was not directly associated with prevalence of HIV-1 discordance, but was modestly correlated with national HIV-1 counseling and testing rates and access to palliative care/basic health care (râ=â0.74, pâ=â0.09). Conclusions/Significance: HIV-1 discordant couples are a critical target for HIV-1 prevention in Africa. In addition to community prevalence of HIV-1 discordance, national infrastructure for HIV-1 testing and healthcare delivery and effective community outreach strategies impact recruitment of HIV-1 discordant couples into HIV-1 prevention trials
Molecular diagnosis of bird-mediated pest consumption in tropical farmland
Biodiversity loss will likely have surprising and dramatic consequences for human wellbeing. Identifying species that benefit society represents a critical first step towards predicting the consequences of biodiversity loss. Though natural predators prevent billions of dollars in agricultural pest damage annually, characterizing which predators consume pests has proven challenging. Emerging molecular techniques may illuminate these interactions. In the countryside of Costa Rica, we identified avian predators of coffeeâs most damaging insect pest, the coffee berry borer beetle (Coleoptera:Scolytidae Hypothenemus hampeii), by assaying 1430 fecal samples of 108 bird species for borer DNA. While feeding trials confirmed the efficacy of our approach, detection rates were low. Nevertheless, we identified six species that consume the borer. These species had narrow diet breadths, thin bills, and short wings; traits shared with borer predators in other systems. Borer predators were not threatened; therefore, safeguarding pest control necessitates managing species beyond those at risk of regional extinction by maintaining populations in farmland habitats. Generally, our results demonstrate potential for pairing molecular methods with ecological analyses to yield novel insights into species interactions. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/2193-1801-3-630) contains supplementary material, which is available to authorized users
Polysomal mRNA Association and Gene Expression in <i>Trypanosoma brucei</i>
Background: The contrasting physiological environments of Trypanosoma brucei procyclic (insect vector) and bloodstream (mammalian host) forms necessitates deployment of different molecular processes and, therefore, changes in protein expression. Transcriptional regulation is unusual in T. brucei because the arrangement of genes is polycistronic; however, genes which are transcribed together are subsequently cleaved into separate mRNAs by trans-splicing. Following pre-mRNA processing, the regulation of mature mRNA stability is a tightly controlled cellular process. While many stage-specific transcripts have been identified, previous studies using RNA-seq suggest that changes in overall transcript level do not necessarily reflect the abundance of the corresponding protein. Methods: To better understand the regulation of gene expression in T. brucei, we performed a bioinformatic analysis of RNA-seq on total, sub-polysomal, and polysomal mRNA samples. We further cross-referenced our dataset with a previously published proteomics dataset to identify new protein coding sequences. Results: Our analyses showed that several long non-coding RNAs are more abundant in the sub-polysome samples, which possibly implicates them in regulating cellular differentiation in T. brucei. We also improved the annotation of the T.brucei genome by identifying new putative protein coding transcripts that were confirmed by mass spectrometry data. Conclusions: Several long non-coding RNAs are more abundant in the sub-polysome cellular fractions and might pay a role in the regulation of gene expression. We hope that these data will be of wide general interest, as well as being of specific value to researchers studying gene regulation expression and life stage transitions in T. brucei
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