The Extragalactic Distance Scale without Cepheids IV

The Cepheid period-luminosity relation is the primary distance indicator used in most determinations of the Hubble constant. The tip of the red giant branch (TRGB) is an alternative basis. Using the new ANU SkyMapper Telescope, we calibrate the Tully Fisher relation in the I band. We find that the TRGB and Cepheid distance scales are consistent.Comment: ApJ in press 201

Investigation Into the Extent and Mechanisms of Gloving and Un-mixed Resin in Fully Encapsulated Roof Bolts

Effective strata control, utilising fully encapsulated roof bolts is dependent on the installed quality of the reinforcement elements. One mechanism by which roof bolts may become less than fully efficient is by glove fingering (gloving) and un-mixing of the resin. Following a routine installed bolt quality audit and some small roof failures containing gloved bolts, a work programme was initiated to determine the extent of the gloving and un-mixing problem and to develop an understanding of mechanisms involved. Results have shown that gloving and un-mixing is a systematic and widespread pheno mena, occurring across the range of resin and/or bolt manufacturers, and in a variety of roof types. Gloving was found in bolts installed using either hand held pneumatic or continuous miner mounted hydraulic bolting rigs, under run of mine (ROM) condition s by operators, and under controlled manufactures “best practice” conditions

Optimal placement of a limited number of observations for period searches

Robotic telescopes present the opportunity for the sparse temporal placement of observations when period searching. We address the best way to place a limited number of observations to cover the dynamic range of frequencies required by an observer. We show that an observation distribution geometrically spaced in time can minimise aliasing effects arising from sparse sampling, substantially improving signal detection quality. The base of the geometric series is however a critical factor in the overall success of this strategy. Further, we show that for such an optimal distribution observations may be reordered, as long as the distribution of spacings is preserved, with almost no loss of quality. This implies that optimal observing strategies can retain significant flexibility in the face of scheduling constraints, by providing scope for on-the-fly adaptation. Finally, we present optimal geometric samplings for a wide range of common observing scenarios, with an emphasis on practical application by the observer at the telescope. Such a sampling represents the best practical empirical solution to the undersampling problem that we are aware of. The technique has applications to robotic telescope and satellite observing strategies, where target acquisition overheads mean that a greater total target exposure time (and hence signal-to-noise) can often in practice be achieved by limiting the number of observations.Comment: 8 pages with 16 figure

A Proper Motion Survey for White Dwarfs with the Wide Field Planetary Camera 2

We have performed a search for halo white dwarfs as high proper motion objects in a second epoch WFPC2 image of the Groth-Westphal strip. We identify 24 high proper motion objects with mu > 0.014 ''/yr. Five of these high proper motion objects are identified as strong white dwarf candidates on the basis of their position in a reduced proper motion diagram. We create a model of the Milky Way thin disk, thick disk and stellar halo and find that this sample of white dwarfs is clearly an excess above the < 2 detections expected from these known stellar populations. The origin of the excess signal is less clear. Possibly, the excess cannot be explained without invoking a fourth galactic component: a white dwarf dark halo. We present a statistical separation of our sample into the four components and estimate the corresponding local white dwarf densities using only the directly observable variables, V, V-I, and mu. For all Galactic models explored, our sample separates into about 3 disk white dwarfs and 2 halo white dwarfs. However, the further subdivision into the thin and thick disk and the stellar and dark halo, and the subsequent calculation of the local densities are sensitive to the input parameters of our model for each Galactic component. Using the lowest mean mass model for the dark halo we find a 7% white dwarf halo and six times the canonical value for the thin disk white dwarf density (at marginal statistical significance), but possible systematic errors due to uncertainty in the model parameters likely dominate these statistical error bars. The white dwarf halo can be reduced to around 1.5% of the halo dark matter by changing the initial mass function slightly. The local thin disk white dwarf density in our solution can be made consistent with the canonical value by assuming a larger thin disk scaleheight of 500 pc.Comment: revised version, accepted by ApJ, results unchanged, discussion expande

The transcriptional repressor Blimp1 is expressed in rare luminal progenitors and is essential for mammary gland development

Mammary gland morphogenesis depends on a tight balance between cell proliferation, differentiation and apoptosis, to create a defined functional hierarchy within the epithelia. The limited availability of stem cell/progenitor markers has made it challenging to decipher lineage relationships. Here, we identify a rare subset of luminal progenitors that express the zinc finger transcriptional repressor Blimp1, and demonstrate that this subset of highly clonogenic luminal progenitors is required for mammary gland development. Conditional inactivation experiments using K14-Cre and WAPi-Cre deleter strains revealed essential functions at multiple developmental stages. Thus, Blimp1 regulates proliferation, apoptosis and alveolar cell maturation during puberty and pregnancy. Loss of Blimp1 disrupts epithelial architecture and lumen formation both in vivo and in three-dimensional (3D) primary cell cultures. Collectively, these results demonstrate that Blimp1 is required to maintain a highly proliferative luminal subset necessary for mammary gland development and homeostasis

The distance of M33 and the stellar population in its outskirts

We present deep V,I photometry of two $9.4' x 9.4' field in the outer regions of the M33 galaxy. We obtain a robust detection of the luminosity of the Red Giant Branch Tip (I{TRGB}=20.72 +- 0.08) from which we derived a new estimate of the distance modulus of M33, (m-M)_0=24.64 +- 0.15, corresponding to a distance D=847 +- 60 Kpc. By comparison of the color and magnitude of the observed Red Giant Branch stars with ridge lines of template globular clusters we obtained the photometric metallicity distribution of the considered fields in three different metallicity scales. The derived metallicity distributions are very similar over a range of distances from the galactic center 10' <= R <= 33', and are characterized by a well defined peak at [M/H] ~ -0.7 ([Fe/H] ~ -1.0, in the Zinn & West scale) and a weak metal-poor tail reaching [M/H] ~ -2.0. Our observations demonstrate that Red Giant Branch and Asymptotic Giant Branch stars have a radial distribution that is much more extended than the young MS stars associated with the star-forming disc.Comment: 10 pages,10 figures,accepted for publication in Astronomy & Astrophysic

Characterization of separability and entanglement in (2×D)(2\times{D})- and (3×D)(3\times{D})-dimensional systems by single-qubit and single-qutrit unitary transformations

We investigate the geometric characterization of pure state bipartite entanglement of $(2\times{D})$- and $(3\times{D})$-dimensional composite quantum systems. To this aim, we analyze the relationship between states and their images under the action of particular classes of local unitary operations. We find that invariance of states under the action of single-qubit and single-qutrit transformations is a necessary and sufficient condition for separability. We demonstrate that in the $(2\times{D})$-dimensional case the von Neumann entropy of entanglement is a monotonic function of the minimum squared Euclidean distance between states and their images over the set of single qubit unitary transformations. Moreover, both in the $(2\times{D})$- and in the $(3\times{D})$-dimensional cases the minimum squared Euclidean distance exactly coincides with the linear entropy (and thus as well with the tangle measure of entanglement in the $(2\times{D})$-dimensional case). These results provide a geometric characterization of entanglement measures originally established in informational frameworks. Consequences and applications of the formalism to quantum critical phenomena in spin systems are discussed.Comment: 8 pages, 1 figur

Mitochondrial Function as a Potential Tool for Assessing Function, Quality and Adulteration in Medicinal Herbal Teas

Quality control has been a significant issue in herbal medicine since herbs became widely used to heal. With the advent of modern technologies, evaluating the quality of medicinal herbs has become more sophisticated but so have the methods of adulterating them. In this paper we undertook a comprehensive literature search to identify the key analytical techniques used in the quality assurance of herbal medicine, reviewing their uses and limitations. We also present a new tool, based on mitochondrial profiling, that can be used to measure medicinal herbal quality. Besides being fundamental to the energy metabolism required for most cellular activities, mitochondria play a direct role in cellular signalling, apoptosis, stress responses, inflammation, cancer, ageing, and neurological function, mirroring some of the most common reasons people take herbal medicines. A fingerprint of the specific mitochondrial effects of medicinal herbs can be documented in order to assess their potential efficacy, detect adulterations that modulate these effects and determine the relative potency of batches. Furthermore, through this method it will be possible to assess whole herbs or complex formulas thus avoiding the issues inherent in identifying active ingredients which may be complex or unknown. Thus, while current analytical methods focus on determining the chemical quality of herbal medicines, including adulteration and contamination, mitochondrial functional analysis offers a new way of determining the quality of plant derived products that is more closely linked to the biological activity of a product and its potential clinical effectiveness

A rich, nearby galaxy cluster in Sagittarius

We report on the existence of a rich, heavily obscured galaxy cluster (or a set of clusters) in Sagittarius, centered near l_(II) ≃ 359°, b_(II) ≃ 8°. About 30 objects, originally identified by Terzan and his collaborators, were imaged and followed up spectroscopically. We present here redshifts for 21 galaxies found among them. There is a pronounced concentration at cz ≃ 8600 km/s (containing 14 out of the 21 galaxies), and another possible concentration near cz ≃ 11 340 km/s (five out of 21). This putative Sagittarius cluster is probably part of a larger system, involving the Ophiuchus cluster at l_(II) ≃ 1°, b_(II) ≃ 9°, cz ≃ 8400 km/s, previously identified by Johnston et al. and Wakamatsu and Malkan. The heavy extinction on this line of sight (we estimate A_v ~ 5^m from the observed Balmer decrements) suggests that there is a much larger, as yet undetected number of galaxies in this direction. We propose that the Sagittarius-Ophiuchus concentration may be a massive cluster, or even a supercluster, comparable in richness to the Coma-A 1367 system, and at a comparable distance. The existence of this concentration serves as a reminder that some possibly dynamically important constituents of the local large-scale structure may be absent from the galaxy .catalogs available now, and the dynamical analyses based on them

Cannabidiol modulates mitochondrial redox and dynamics in MCF7 cancer cells: a study using fluorescence lifetime imaging microscopy of NAD(P)H

The cannabinoid, cannabidiol (CBD), is part of the plant's natural defence system that when given to animals has many useful medicinal properties, including activity against cancer cells, modulation of the immune system, and efficacy in epilepsy. Although there is no consensus on its precise mode of action as it affects many cellular targets, CBD does appear to influence mitochondrial function. This would suggest that there is a cross-kingdom ability to modulate stress resistance systems that enhance homeostasis. As NAD(P)H autofluorescence can be used as both a metabolic sensor and mitochondrial imaging modality, we assessed the potential of this technique to study the in vitro effects of CBD using 2-photon excitation and fluorescence lifetime imaging microscopy (2P-FLIM) of NAD(P)H against more traditional markers of mitochondrial morphology and cellular stress in MCF7 breast cancer cells. 2P-FLIM analysis revealed that the addition of CBD induced a dose-dependent decrease in bound NAD(P)H, with 20 µM treatments significantly decreasing the contribution of bound NAD(P)H by 14.6% relative to control (p<0.001). CBD also increased mitochondrial concentrations of reactive oxygen species (ROS) (160 ± 53 vs. 97.6 ± 4.8%, 20 µM CBD vs. control, respectively, p<0.001) and Ca2+ (187 ± 78 vs. 105 ± 10%, 20 µM CBD vs. control, respectively, p<0.001); this was associated with significantly decreased mitochondrial branch length and increased fission. These are all suggestive of mitochondrial stress. Our results support the use of NAD(P)H autofluorescence as an investigative tool and provide further evidence that CBD can modulate mitochondrial function and morphology in a dose dependent manner, with clear evidence of it inducing oxidative stress at higher concentrations. This continues to support emerging data in the literature and may provide further insight into its overall mode of action, not only in cancer, but potentially its function in the plant and why it can act as a medicine