Capacités de traitement d'un effluent de synthèse en infiltration percolation

Dans cet article, nous présentons des travaux mettant en évidence les capacités de traitement biologique des eaux résiduaires urbaines au sein des milieux poreux de textures différentes. Une étude comparative du développement de la biomasse couplé aux mécanismes généraux de transferts gazeux à travers deux réacteurs biologiques est menée. Des lits d’infiltration percolation sont simulés par des colonnes garnies de sables d’origine et de structures différentes. Ils sont alimentés périodiquement via un automate de commande avec un influent d’une charge de 525 mgDCO/l et de 54 mgNK/l. Les résultats obtenus établissent le fait qu’un développement équilibré de la biomasse incluant les phases de croissance et de régression est intrinsèquement lié à la nature physique du matériau support. A l’aide des carottes prélevées sur les massifs filtrants et des sondes d’oxymétrie introduites à différentes hauteurs des lits d’infiltration, nous montrons que la répartition verticale du biofilm dans les colonnes ainsi que l’oxygénation des milieux poreux lors des périodes de repos sont également corrélées à la structure des supports pourtant de diamètres moyens similaires. L’efficacité de traitement biologique du carbone est supérieure pour un sable d’origine alluvionnaire comparativement à un sable concassé ; la tendance s’inversant significativement lorsqu’il s’agit de la diminution de l’azote.In this article, we present work highlighting the capacity of variously textured porous media to biologically process urban waste water. A comparative study was undertaken that coupled biomass development with general gas transfer mechanisms through two biological engines.Infiltration/percolation beds are biological systems that treat water using a purifying bacterial culture that develops on a mineral support. Used in domestic wastewater treatment, they are regarded as being well suited to rural areas. These infiltration/percolation beds are easy to use and attain a high quality in their output, two factors that constitute assets for small communities.Infiltration/percolation beds were simulated in the present work by columns lined with sands of varying origin and structure. They were fed automatically, at set intervals, with waste water containing 525 mg/L of dissolved organic carbon (DOC) and 54 mg/L of Kjeldahl nitrogen. A balanced development of the biomass, including the phases of growth and regression, was intrinsically related to the physical nature of the material used as a support. First, during the supply period, balanced growth of the biomass was quickly reached within the crushed sand. Secondly, within round sand, the regression of the biofilm was less significant and more regular over several weeks. In both supports, the regression was well correlated with an exponential decay. Lastly, the frequency of the supply periods, the organic loads involved, and the rest periods imposed are all factors that contribute to a lack of accumulation of living and/or inert organic matter in the columns.After obtaining a balanced development of the biomass, the abilities of the columns to reduce the concentrations of carbon, Kjeldahl nitrogen and ammonia were evaluated. Samples of effluent were taken downstream, both before the beginning of the supply period and as it came out of the column approximately thirty minutes after beginning the drainage period. The percentage of suspended matter coming out of the columns gave rather precise information on the scrubbing of the solid mass caused by various shearing speeds or by the structure of the base.Generally, it appeared that :- The reduction of the overall DOC was higher than 70%, regardless of which type of sand base was used.- In the water coming out of the column made up of round sand, the overall DOC content was without exception lower than 125 mg/L, as would be expected for an infiltration-percolation process.- The treatment of carbon in the columns based on stream sand was in general more effective than that obtained in columns with crushed sand.Concerning the treatment of nitrogen, crushed sand yielded outputs with a reduction in ammonia that was a lot higher than 80% (mean ± SD: 92 ± 4%) and was systematically higher than those obtained with stream sand (mean ± SD: 72 ± 7%). The suspended matter content was extremely low in the effluent; since no suspended matter was introduced into the effluent, the concentration coming out of the column was the direct result of the biofilm becoming detached and/or, to a lesser extent, of the transport of the biomass in the liquid phase. As could be expected, because of the roughness causing more shearing, more particles became detached when the interior solid mass was made up of crushed sand.Using core samples taken from the filter's solid mass, as well as oxygen probes inserted at various levels into the biological engines, we showed that the vertical distribution of the biofilm in the columns, as well as the oxygenation of the porous media during the rest periods, were all correlated with the structure of the solid supports (note that all sands had similar average diameters). Gaseous exchanges within the filter's solid mass were dependent on both the type of coating and the depth at which they took place. The oxygen probes were inserted at depths of 14 cm and 18 cm respectively, to determine the percentage of oxygen saturation in the liquid phase within the filter during one week while the columns were operating. Two phases were characteristic of the exchanges observed in the upper part of the filter's solid mass. First we noted an instantaneous reduction in the oxygen content of the liquid phase, linked to the arrival of a batch, followed by a reoxygenation of this portion. Next there was a long phase, lasting approximately 94% of the time between batches, during which time the oxygen content in the residual moisture remained constant. In the lower part of the solid mass, variation in oxygen content was different. Partial deoxygenation in the liquid phase of the porous media here was primarily due to the biochemical oxidation of the organic matter (i.e., consumption by the biomass). It was also due, to a lesser extent, to the augmentation in moisture content that occurs after each batch, and leads to a reduction in porosity and a decrease in oxygen transfer. During the final phase of drainage, a balance was created between the open porous space with air, and the space containing stagnant moisture. The diffusion of oxygen in the former and its transfer towards the latter thus compensated for this consumption, and it therefore remained constant. If the medium was left to dry out longer, the diffusion and transfer phenomena increased, and we observed an increase in oxygen content.With respect to the vertical distribution of biomass in the columns, we found that it was almost homogeneous inside the stream sand, up to a depth of 28 cm (total depth = 40 cm). Generally, the organic matter content at each point of measurement in the column was higher in the stream sand than in the crushed sand. The variation was greater around 28 cm, where there was a more significant quantity of moisture inside the sand

EFISIENSI PAKAN IKAN NILA (Oreochromis niloticus) YANG DIBUDIDAYAKAN DI SISTEM AKUAPONIK

Teknologi akuaponik merupakan gabungan antara sistem hidroponik dan akuakultur yang dapat mengatasi persoalan kualitas air dan optimalisasi lahan untuk peningkatan kualitas produksi. Penelitian ini bertujuan untuk mengetahui efisiensi pakan ikan nila yang dibudidayakan di kolam akuaponik dan kolam konvesional. Penelitian dilaksanakan selama 30 hari di Jurusan Perikanan dan Kebaharian Politeknik negeri nusa utara. Tahapan dalam penelitian antara lain persiapan bahan sistem uji dan sistem akuaponik, pemberian pakan, pengukuran efisiensi pakan dan analsis data. Hasil penelitian menunjukkan efisiensi pakan ikan nila dikolam akuaponik lebih rendah dibandingkan kolam konvensional

A novel protein isoform of the RON tyrosine kinase receptor transforms human pancreatic duct epithelial cells.

The MST1R gene is overexpressed in pancreatic cancer producing elevated levels of the RON tyrosine kinase receptor protein. While mutations in MST1R are rare, alternative splice variants have been previously reported in epithelial cancers. We report the discovery of a novel RON isoform discovered in human pancreatic cancer. Partial splicing of exons 5 and 6 (P5P6) produces a RON isoform that lacks the first extracellular immunoglobulin-plexin-transcription domain. The splice variant is detected in 73% of xenografts derived from pancreatic adenocarcinoma patients and 71% of pancreatic cancer cell lines. Peptides specific to RON P5P6 detected in human pancreatic cancer specimens by mass spectrometry confirm translation of the protein isoform. The P5P6 isoform is found to be constitutively phosphorylated, present in the cytoplasm, and it traffics to the plasma membrane. Expression of P5P6 in immortalized human pancreatic duct epithelial (HPDE) cells activates downstream AKT, and in human pancreatic epithelial nestin-expressing cells, activates both the AKT and MAPK pathways. Inhibiting RON P5P6 in HPDE cells using a small molecule inhibitor BMS-777607 blocked constitutive activation and decreased AKT signaling. P5P6 transforms NIH3T3 cells and induces tumorigenicity in HPDE cells. Resultant HPDE-P5P6 tumors develop a dense stromal compartment similar to that seen in pancreatic cancer. In summary, we have identified a novel and constitutively active isoform of the RON tyrosine kinase receptor that has transforming activity and is expressed in human pancreatic cancer. These findings provide additional insight into the biology of the RON receptor in pancreatic cancer and are clinically relevant to the study of RON as a potential therapeutic target

Ha-Ketuba be-Histalselota ha-Historit

Copia digital. Madrid : Ministerio de Cultura. Subdirección General de Coordinación Bibliotecaria, 201

Ipteks Pelaporan Persediaan pada Puprd Sulut

Government Regulation No. 71 of 2010 concerning Government Accounting Standards in recording inventory can realize the performance performed by government agencies, especially in the presentation of inventory reporting. The purpose of this study was to determine the government accounting standard policies in reporting inventory at the Office of Public Works and Regional Spatial Planning of North Sulawesi Province according to Government Regulation Nomber. 71 of 2010 based on PSAP 05 concerning Inventory Accounting and the method used in this research is the service inventory reporting analysis method Public Works and Regional Spatial Planning of North Sulawesi Province with the technique of science and technology collecting inventory data and conducting analysis. The results of this study indicate that inventory reports at the Public Works Agency and Regional Spatial Planning of North Sulawesi Province are stated to be in accordance with government accounting standards

Consumers’ salient beliefs regarding foods from edible insects in Kenya: a qualitative study using concepts from the theory of planned behaviour

Population growth, urbanization and climate change, are among the factors that have created uncertainties and pressures on current global food and economic systems. Agricultural intensification can no longer support the increasing demand for food, especially of protein origin. Consequently, pressure is mounting on the supply side (private sector) to develop viable alternative sources of protein’ foods. Insects as food and consumption of foods from edible insects (FEI) are being promoted as one potential solution to the declining access to protein foods. However, one of the challenges facing the private sector is to demonstrate the efficacy of FEI programmes in the face of limited information regarding consumer - psychographic characteristics including their attitudes, values, interests and beliefs. The aim of the study was to explore the salient beliefs underlying consumer attitudes towards FEI consumption in Kenya. To achieve this objective, six focus group discussions (FGDs) were conducted (n= 43), three with only female participants and the other three with a mixed gender. The FGD script was coded using the Theory of Planned Behaviour theoretical framework. The results show that, salient beliefs related to taste, availability, convenience, affordability and other benefits beyond nutrition, are the main determinants of the participants’ intentions to consume FEI. Intentions are also influenced by the perceived feelings regarding the social referents, including family members, peers, religious leaders and health officials; and perceived behavioural control factors such as perceived risks on their food choices, perceived convenience and availability. While designing local programmes to promote FEI consumption, participants support the idea of small groups, but expresses concern about the time of day (evening preferred), length and location of the group session. However, many participants are not receptive to receiving telephone messages, although they are open to the idea of receiving phone calls. The study findings provide unique insights, among them, cultural beliefs that underlies consumer attitudes towards consuming FEI. Additionally, the results suggest possible approaches and practical interventions that can be used to promote FEI consumption in specific regions of Kenya.Key words: Foods from edible insects, theory of planned behaviour, Salient belief

Some basement rocks from Bear Mountain to the Housatonic Highlands

Guidebook for field trips in western Massachusetts, northern Connecticut and adjacent areas of New York: 67th annual meeting October 10, 11, and 12, 1975: Trip A-1; C-

Integrated RNA and DNA sequencing improves mutation detection in low purity tumors

Identifying somatic mutations is critical for cancer genome characterization and for prioritizing patient treatment. DNA whole exome sequencing (DNA-WES) is currently the most popular technology; however, this yields low sensitivity in low purity tumors. RNA sequencing (RNA-seq) covers the expressed exome with depth proportional to expression. We hypothesized that integrating DNA-WES and RNA-seq would enable superior mutation detection versus DNA-WES alone. We developed a first-of-its-kind method, called UNCeqR, that detects somatic mutations by integrating patient-matched RNA-seq and DNA-WES. In simulation, the integrated DNA and RNA model outperformed the DNA-WES only model. Validation by patient-matched whole genome sequencing demonstrated superior performance of the integrated model over DNA-WES only models, including a published method and published mutation profiles. Genome-wide mutational analysis of breast and lung cancer cohorts (n = 871) revealed remarkable tumor genomics properties. Low purity tumors experienced the largest gains in mutation detection by integrating RNA-seq and DNA-WES. RNA provided greater mutation signal than DNA in expressed mutations. Compared to earlier studies on this cohort, UNCeqR increased mutation rates of driver and therapeutically targeted genes (e.g. PIK3CA, ERBB2 and FGFR2). In summary, integrating RNA-seq with DNA-WES increases mutation detection performance, especially for low purity tumors

Differences in seasons and rice varieties provide opportunities for improving nitrogen use efficiency and management in irrigated rice in Kenya

Open Access JournalApart from nitrogen (N) rates, N use efficiency (NUE) (yield N/total input N) is affected by seasons, crop developmental stages, and varieties. Knowledge of how these factors affect NUE in rice production in Kenya is limited. Therefore, field experiments were conducted with 'low rates' of N (simulating farmers' practices) of 0, 26, 52 and 78 kg N ha−1 with five varieties (MWUR1, MWUR4, IRAT109, NERICA4 and NERICA10) and higher rates of N (125, 175, and 225 kg N ha−1) simulating researchers' doses with two lowland varieties (Basmati 370 and BW 196) and IR 72. Another experiment on NUE responses to sites, N rates and dose (split or full dose) was undertaken with the IR97 variety. With the 'low rate', yields increased with incremental N rates up to 52 Kg N ha−1 and declined (during cold periods, for some varieties). In this scenario, the N agronomic efficiencies (AEN) declined with increasing N but depended on sites and seasons. However, most AEN values were above 100, implying nutrient mining. In most cases (except at the Mwea site), the N utilization efficiency (NUtE) ranged from 16 to 22kg kg−1 and were not significantly affected by sources and methods of N application. In all cases, an increase in N elicited declining trends in NUtE. Moreover, N uptake efficiency ranged between 22 and 90kg kg−1 without significant variation among varieties. For the 'high N rates', high biomass yield resulted in higher grain yields in BW 196 and IR 72 but yield declined beyond 75 kg ha−1 N rates due to poor grain filling, particularly when a cold period coincided with booting and grain filling. We conclude that N rates, doses and rice varieties are key determinants of AEN and NUtE in contrasting rice growing seasons in Kenya. Cropping seasons and rice varieties are therefore potential key determinants of sustainable rice productivity and improved NUE in rice-based systems in the studied regions of Kenya