Algorithms for identification and categorization

The main features of a family of efficient algorithms for recognition and classification of complex patterns are briefly reviewed. They are inspired in the observation that fast synaptic noise is essential for some of the processing of information in the brain.Comment: 6 pages, 5 figure

Fuzzy Free Path Detection based on Dense Disparity Maps obtained from Stereo Cameras

In this paper we propose a fuzzy method to detect free paths in real-time using digital stereo images. It is based on looking for linear variations of depth in disparity maps, which are obtained by processing a pair of rectified images from two stereo cameras. By applying least-squares fitting over groups of disparity maps columns to a linear model, free paths are detected by giving a certainty using a fuzzy rule. Experimental results on real outdoor images are also presented.Nuria Ortigosa acknowledges the support of Universidad Polit'ecnica de Valencia under grant FPI-UPV 2008. Samuel Morillas acknowledges the support of Spanish Ministry of Education and Science under grant MTM 2009-12872-C02-01.Ortigosa Araque, N.; Morillas Gómez, S.; Peris Fajarnes, G.; Dunai Dunai, L. (2012). Fuzzy Free Path Detection based on Dense Disparity Maps obtained from Stereo Cameras. 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Deciphering past and present atmospheric metal pollution of urban environments: The role of black crusts formed on historical constructions

Construction materials affected by black crusts (BCs) can be subjected to restoration, demolition, recycling or even to their management as waste products. Therefore, the determination of their chemical features should be considered a crucial step before undertaking any action. In this work, we present the development of an analytical methodology useful to be implemented as a routine screening tool to detect recent and past atmospheric emissions of heavy metals, nowadays superficially deposited or even encapsulated in BCs. For its development, BCs together with the underneath original substrate/construction material were sampled from the historical construction Punta Begona Galleries (Getxo, Basque Country, North of Spain). In order to detect quickly and in a cost-effective way the stratification of the metallic deposits in the BCs over time (surface or external/recent and internal/past), thin sections were analyzed by elemental spectroscopic imaging techniques (SEM-EDS and mu-ED-XRF). In the external part of the BCs, iron particles were mainly identified, whereas in the inner areas (past deposition events) of the most exposed BCs to the atmosphere, lead accumulations together with zinc and copper were identified. Additional Raman imaging studies allowed to perform the molecular speciation study of lead, identifying mainly laurionite (PbClOH) together with hydrocerussite (Pb-3(CO3)(2)(OH)(2)). The presence of the mentioned lead chloride hydroxide confirms the role of the marine aerosol (chloride input) in the formation of the metallic compounds. These experimental evidences were used to assist the chemical equilibrium models developed to explain the reactivity pathway, which lead to the formation of the identified compounds. Through ICP-MS and lead isotopic ratio analysis, more than 3000 mg kg(-1) of lead were quantified in the BCs, probably coming from the old emissions conducted by the old power station close to the construction,. That lead content can be high enough to consider those crusts as a source of metallic contamination and a possible risk to the environment and human health

performance over time and durability assessment of external thermal insulation systems with artificial stone cladding

The contribution describes an experimental programme on durability assessment using the accelerated ageing of an outer wall component consisting of plasterboard support, an external thermal insulation composite system (ETICS) in polystyrene and a cladding that was realised half with natural stone and the other half with cast stone. The sample was placed in front of the climatic chamber by means of a special frame, in the so-called door configuration. During the accelerated ageing, this sample was simultaneously monitored over time using temperatures probes and flowmeter tests in order to evaluate the decay of the thermal performance over time. The experimental research was conducted with the aim of assessing the decay in thermal performance of an ETICS covered with artificial stone, comparing it with a similar stratigraphy but with a natural stone cladding

Visceral leishmaniasis caused by Leishmania infantum in a Spanish patient in Argentina: What is the origin of the infection? Case report

BACKGROUND: The question "Where have you been?" is a common one asked by doctors in Northern Europe and America when faced with clinical symptoms not typical of their country. This question must also arise in the clinics of developing countries in which non-autochthonous cases such as the one described here can appear. Important outbreaks of Leishmania infantum have been recorded in the last decade in several Latin American countries but its presence has not yet been recorded in Argentina. We report the first case of visceral leishmaniasis owing to L. infantum in this country. CASE PRESENTATION: A 71-year-old Spanish woman who has been living in Mendoza, Argentina, during the last 40 years presented with a history of high fever and shivering, anemia, leukopenia and splenomegaly over two years. Argentinian doctors did not suspect visceral leishmaniasis even when the histological analysis revealed the presence of "intracytoplasmatic spheroid particles compatible with fungal or parasitic infection". After a serious deterioration in her health, she was taken to Spain where she was evaluated and visceral leishmaniasis was established. Specific identification of the parasite was done by PCR-ELISA, isoenzyme electrophoresis and RAPD-PCR. CONCLUSION: We would like to point out that: i) cases such as the one described here, which appear in non-endemic areas, can pass unnoticed by the clinical physician. ii) in countries in which these introduced cases reside, in-depth parasitological studies are required into vectors and possible reservoirs to rule out the rare case of local infection and, once infection has taken place, to ensure that this does not spread by anthroponotic transmission or a competent reservoir

Phlebotomine sand fly survey in the focus of leishmaniasis in Madrid, Spain (2012-2014): seasonal dynamics, Leishmania infantum infection rates and blood meal preferences

BACKGROUND: An unusual increase of human leishmaniasis cases due to Leishmania infantum is occurring in an urban area of southwestern Madrid, Spain, since 2010. Entomological surveys have shown that Phlebotomus perniciosus is the only potential vector. Direct xenodiagnosis in hares (Lepus granatensis) and rabbits (Oryctolagus cuniculus) collected in the focus area proved that they can transmit parasites to colonized P. perniciosus. Isolates were characterized as L. infantum. The aim of the present work was to conduct a comprehensive study of sand flies in the outbreak area, with special emphasis on P. perniciosus. METHODS: Entomological surveys were done from June to October 2012-2014 in 4 stations located close to the affected area. Twenty sticky traps (ST) and two CDC light traps (LT) were monthly placed during two consecutive days in every station. LT were replaced every morning. Sand fly infection rates were determined by dissecting females collected with LT. Molecular procedures applied to study blood meal preferences and to detect L. infantum were performed for a better understanding of the epidemiology of the outbreak. RESULTS: A total of 45,127 specimens belonging to 4 sand fly species were collected: P. perniciosus (75.34%), Sergentomyia minuta (24.65%), Phlebotomus sergenti (0.005%) and Phlebotomus papatasi (0.005%). No Phlebotomus ariasi were captured. From 3203 P. perniciosus female dissected, 117 were infected with flagellates (3.7%). Furthermore, 13.31% and 7.78% of blood-fed and unfed female sand flies, respectively, were found infected with L. infantum by PCR. The highest rates of infected P. perniciosus were detected at the end of the transmission periods. Regarding to blood meal preferences, hares and rabbits were preferred, although human, cat and dog blood were also found. CONCLUSIONS: This entomological study highlights the exceptional nature of the Leishmania outbreak occurring in southwestern Madrid, Spain. It is confirmed that P. perniciosus is the only vector in the affected area, with high densities and infection rates. Rabbits and hares were the main blood meal sources of this species. These results reinforce the need for an extensive and permanent surveillance in this region, and others of similar characteristics, in order to control the vector and regulate the populations of wild reservoirs.This study was partially sponsored and funded by: Dirección General de Salud Pública, Consejería de Sanidad, Comunidad de Madrid; Colegio de Veterinarios de Madrid; Colegio de Biólogos de Madrid and EU grant FP7-261504 EDENext (http://www.edenext.eu).S

Population specificity of the DNAI1 gene mutation spectrum in primary ciliary dyskinesia (PCD)

<p>Abstract</p> <p>Background</p> <p>Mutations in the <it>DNAI1 </it>gene, encoding a component of outer dynein arms of the ciliary apparatus, are the second most important genetic cause of primary ciliary dyskinesia (PCD), the genetically heterogeneous recessive disorder with the prevalence of ~1/20,000. The estimates of the <it>DNAI1 </it>involvement in PCD pathogenesis differ among the reported studies, ranging from 4% to 10%.</p> <p>Methods</p> <p>The coding sequence of <it>DNAI1 </it>was screened (SSCP analysis and direct sequencing) in a group of PCD patients (157 families, 185 affected individuals), the first ever studied large cohort of PCD patients of Slavic origin (mostly Polish); multiplex ligation-dependent probe amplification (MLPA) analysis was performed in a subset of ~80 families.</p> <p>Results</p> <p>Three previously reported mutations (IVS1+2-3insT, L513P and A538T) and two novel missense substitutions (C388Y and G515S) were identified in 12 families (i.e. ~8% of non-related Polish PCD patients). The structure of background SNP haplotypes indicated common origin of each of the two most frequent mutations, IVS1+2-3insT and A538T. MLPA analysis did not reveal any significant differences between patients and control samples. The Polish cohort was compared with all the previously studied PCD groups (a total of 487 families): IVS1+2-3insT remained the most prevalent pathogenetic change in <it>DNAI1 </it>(54% of the mutations identified worldwide), and the increased global prevalence of A538T (14%) was due to the contribution of the Polish cohort.</p> <p>Conclusions</p> <p>The worldwide involvement of <it>DNAI1 </it>mutations in PCD pathogenesis in families not preselected for ODA defects ranges from 7 to 10%; this global estimate as well as the mutation profile differs in specific populations. Analysis of the background SNP haplotypes suggests that the increased frequency of chromosomes carrying A538T mutations in Polish patients may reflects local (Polish or Slavic) founder effect. Results of the MLPA analysis indicate that no large exonic deletions are involved in PCD pathogenesis.</p