Identification of a mutation in the para-sodium channel gene of the cattle tick Rhipicephalus (Boophilus) microplus associated with resistance to synthetic pyrethroid acaricides

Resistance against synthetic pyrethroid (SP) products for the control of cattle ticks in Australia was detected in the field in 1984, within a very short time of commercial introduction. We have identified a mutation in the domain II S4-5 linker of the para-sodium channel that is associated with resistance to SPs in the cattle tick Rhipicephalus (Boophilus) microplus from Australia. The cytosine to adenine mutation at position 190 in the R. microplus sequence AF134216, results in an amino acid substitution from leucine in the susceptible strain to isoleucine in the resistant strain. A similar mutation has been shown to confer SP resistance in the whitefly, Bemisia tabaci, but has not been described previously in ticks. A diagnostic quantitative PCR assay has been developed using allele-specific Taqman® minor groove-binding (MGB) probes. Using the assay to screen field and laboratory populations of ticks showed that homozygote allelic frequencies correlated highly with the survival percentage at the discriminating concentration of cypermethrin

New approaches to the modelling of multi-component fuel droplet heating and evaporation

The previously suggested quasi-discrete model for heating and evaporation of complex multi-component hydrocarbon fuel droplets is described. The dependence of density, viscosity, heat capacity and thermal conductivity of liquid components on carbon numbers n and temperatures is taken into account. The effects of temperature gradient and quasi-component diffusion inside droplets are taken into account. The analysis is based on the Effective Thermal Conductivity/Effective Diffusivity (ETC/ED) model. This model is applied to the analysis of Diesel and gasoline fuel droplet heating and evaporation. The components with relatively close n are replaced by quasi-components with properties calculated as average properties of the a priori defined groups of actual components. Thus the analysis of the heating and evaporation of droplets consisting of many components is replaced with the analysis of the heating and evaporation of droplets consisting of relatively few quasi-components. It is demonstrated that for Diesel and gasoline fuel droplets the predictions of the model based on five quasi-components are almost indistinguishable from the predictions of the model based on twenty quasi-components for Diesel fuel droplets and are very close to the predictions of the model based on thirteen quasi-components for gasoline fuel droplets. It is recommended that in the cases of both Diesel and gasoline spray combustion modelling, the analysis of droplet heating and evaporation is based on as little as five quasi-components

Systems Analysis in Forestry and Forest Industries

The purpose of this book is to present a variety of articles revealing the state of the art of applications of systems analysis techniques to problems of the forest sector. Such applications cover a vast range of issues in forestry and the forest industry. They include the dynamics of the forest ecosystem, optimal forest management, the roundwood market, forest industrial strategy, regional and national forest sector policy as well as international trade in forest products. Forest industrial applications at mill level, such as optimal paper trimming, cutting, and production scheduling, are however, excluded

Improved spatial resolution of the sodium distribution on Mercury

The distribution of sodium emission over the surface of Mercury is non-uniform, and changes over time. These non-uniformities and time-dependent changes give clues to the processes that produce the sodium. However, observations of the distribution of sodium on Mercury are hampered by the bad seeing that accompanies the need to observe either in daytime, or at extremely large air masses in twilight. In an effort to improve this situation, we have developed an image stabilizer utilizing a piezoelectric driven tip-tilt correction mirror for daytime spectral imaging of Mercury. The system is a modification of one that was originally developed for solar observations at the McMath-Pierce solar telescope. Use of image stabilization results in a noticeable improvement in spatial resolution of our Mercury sodium images. A series of sodium images taken over an eight-day period show changes in which an emission peak in high southern latitudes disappears, and is replaced by an emission peak at high northern latitudes. Further systematic observations and improvements are planned for the image stabilizer system, as well as experimental observations with a low-order adaptive optics system incorporating a commercially available 37-actuator deformable mirror

π+\pi^+ and π0\pi^0 Polarizabilities from {γγ→ππ\gamma\gamma\rightarrow\pi\pi} Data on the Base of S-Matrix Approach

We suggest the most model-independent and simple description of the $\gamma\gamma\rightarrow\pi\pi$ process near threshold in framework of S-matrix approach. The amplitudes contain the pion polarizabilities and rather restricted information about $\pi \pi$ interaction. Application of these formulae for description of MARK-II \cite{M2} and Crystal Ball \cite{CB} data gives: $(\alpha-\beta)^{C}=(6.0\pm 1.2)\cdot 10^{-42} {\rm cm}^{3}$, $(\alpha-\beta)^{N}=(-1.4\pm 2.1)\cdot 10^{-42} cm^3$ (in units system $e^2 = 4 \pi \alpha$) at the experimental values of $\pi \pi$ scattering lengths. Both values are compartible with current algebra predictions.Comment: LaTeX, 14 pages plus 6 figures (not included, available upon request) , ISU-IAP.Th93-03, Irkuts

Simulation of a stationary dark soliton in a trapped zero-temperature Bose-Einstein condensate

We discuss a computational mechanism for the generation of a stationary dark soliton, or black soliton, in a trapped Bose-Einstein condensate using the Gross-Pitaevskii (GP) equation for both attractive and repulsive interaction. It is demonstrated that the black soliton with a "notch" in the probability density with a zero at the minimum is a stationary eigenstate of the GP equation and can be efficiently generated numerically as a nonlinear continuation of the first vibrational excitation of the GP equation in both attractive and repulsive cases in one and three dimensions for pure harmonic as well as harmonic plus optical-lattice traps. We also demonstrate the stability of this scheme under different perturbing forces.Comment: 7 pages, 15 ps figures, Final version accepted in J Low Temp Phy

Photon-Photon Scattering, Pion Polarizability and Chiral Symmetry

Recent attempts to detect the pion polarizability via analysis of $\gamma\gamma\rightarrow\pi\pi$ measurements are examined. The connection between calculations based on dispersion relations and on chiral perturbation theory is established by matching the low energy chiral amplitude with that given by a full dispersive treatment. Using the values for the polarizability required by chiral symmetry, predicted and experimental cross sections are shown to be in agreement.Comment: 21 pages(+10 figures available on request), LATEX, UMHEP-38

Testing the interaction of dark energy to dark matter through the analysis of virial relaxation of clusters Abell Clusters A586 and A1689 using realistic density profiles

Interaction between dark energy and dark matter is probed through deviation from the virial equilibrium for two relaxed clusters: A586 and A1689. The evaluation of the virial equilibrium is performed using realistic density profiles. The virial ratios found for the more realistic density profiles are consistent with the absence of interaction.Comment: 16pp 1 fig; accepted by GeR

Multi-segment coherent beam combining

Scaling laser systems to large sizes for power beaming and other applications can sometimes be simplified by combing a number of smaller lasers. However, to fully utilize this scaling, coherent beam combination is necessary. This requires measuring and controlling each beam`s pointing and phase relative to adjacent beams using an adaptive optical system. We have built a sub-scale brass-board to evaluate various methods for beam-combining. It includes a segmented adaptive optic and several different specialized wavefront sensors that are fabricated using diffractive optics methods. We have evaluated a number of different phasing algorithms, including hierarchical and matrix methods, and have demonstrated phasing of several elements. The system is currently extended to a large number of segments to evaluate various scaling methodologies

Chronic tooth pulp inflammation induces persistent expression of phosphorylated ERK (pERK) and phosphorylated p38 (pp38) in trigeminal subnucleus caudalis

Background: Extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinase are transiently phosphorylated (activated) in the spinal cord and trigeminal nucleus by acute noxious stimuli. Acute stimulation of dental pulp induces short-lived ERK activation in trigeminal subnucleus caudalis (Vc), and p38 inhibition attenuates short-term sensitization in Vc induced by acute pulpal stimulation. We have developed a model to study central changes following chronic inflammation of dental pulp that induces long-term sensitization. Here, we examine the effects of chronic inflammation and acute stimulation on the expression of phosphorylated ERK (pERK), phosphorylated p38 (pp38) and Fos in Vc. Results: Chronic inflammation alone induced bilateral expression of pERK and pp38 in Vc, but did not induce Fos expression. Stimulation of both non-inflamed and inflamed pulps significantly increased pERK and pp38 bilaterally; expression was greatest in inflamed, stimulated animals, and was similar following 10-min and 60-min stimulation. Stimulation for 60 min, but not 10 min, induced Fos in ipsilateral Vc; Fos expression was significantly greater in inflamed, stimulated animals. pERK was present in both neurons and astrocytes; pp38 was present in neurons and other non-neuronal, non-astrocytic cell types. Conclusions: This study provides the first demonstration that chronic inflammation of tooth pulp induces persistent bilateral activation of ERK and p38 within Vc, and that this activation is further increased by acute stimulation. This altered activity in intracellular signaling is likely to be linked to the sensitization that is seen in our animal model and in patients with pulpitis. Our data indicate that pERK and pp38 are more accurate markers of central change than Fos expression. In our model, localization of pERK and pp38 within specific cell types differs from that seen following acute stimulation. This may indicate specific roles for different cell types in the induction and maintenance of pulpitic and other types of pain