724 research outputs found
Human derived feeder fibroblasts for the culture of epithelial cells for clinical use
Aim: To investigate human oral mucosal fibroblasts (HOMF) and human limbal fibroblasts (HLF) as alternatives to murine 3T3 feeder fibroblasts currently used to support epithelial cell expansion for the treatment of limbal epithelial stem cell deficiency.
Methods: HLF and HOMF were compared to 3T3 for their ability to support the culture of human limbal epithelial cells (HLE) and human oral mucosal epithelial cells.
Results: HOMF, but not HLF, were equivalent to 3T3 in terms of the number of epithelial population doublings achieved. HLE co-cultured with HOMF or 3T3 had similar expression of corneal and putative stem cell markers.
Conclusion: HOMF are a suitable and safer feeder fibroblast alternative to 3T3 for the production of epithelial cells for clinical use
Model for nucleation in GaAs homoepitaxy derived from first principles
The initial steps of MBE growth of GaAs on beta 2-reconstructed GaAs(001) are
investigated by performing total energy and electronic structure calculations
using density functional theory and a repeated slab model of the surface. We
study the interaction and clustering of adsorbed Ga atoms and the adsorption of
As_2 molecules onto Ga atom clusters adsorbed on the surface. The stable nuclei
consist of bound pairs of Ga adatoms, which originate either from dimerization
or from an indirect interaction mediated through the substrate reconstruction.
As_2 adsorption is found to be strongly exothermic on sites with a square array
of four Ga dangling bonds. Comparing two scenarios where the first As_2 gets
incorporated in the incomplete surface layer, or alternatively in a new added
layer, we find the first scenario to be preferable. In summary, the
calculations suggest that nucleation of a new atomic layer is most likely on
top of those surface regions where a partial filling of trenches in the surface
has occurred before.Comment: 8 pages, 14 figures, Submitted to Phys. Rev. B (December 15, 1998).
Other related publications can be found at
http://www.fhi-berlin.mpg.de/th/paper.htm
Fast Transition between High-soft and Low-soft States in GRS 1915+105: Evidence for a Critically Viscous Accretion Flow
We present the results of a detailed analysis of RXTE observations of class
which show an unusual state transition between high-soft and low-soft
states in the microquasar GRS 1915+105. Out of about 600 pointed RXTE
observations, the source was found to exhibit such state transition only on 16
occasions. An examination of the RXTE/ASM data in conjunction with the pointed
observations reveals that these events appeared as a series of quasi-regular
dips in two stretches of long duration (about 20 days during each occasions)
when hard X-ray and radio flux were very low. The X-ray light curve and
color-color diagram of the source during these observations are found to be
different from any reported so far. The duration of these dips is found to be
of the order of a few tens of seconds with a repetition time of a few hundred
seconds. The transition between these dips and non-dips which differ in
intensity by a factor of ~ 3.5, is observed to be very fast (~ a few seconds).
It is observed that the low-frequency narrow QPOs are absent in the power
density spectrum (PDS) of the dip and non-dip regions of class and the
PDS is a power law in 0.1 - 10 Hz frequency range. There is a remarkable
similarity in the spectral and timing properties of the source during the dip
and non-dip regions in these set of observations. These properties of the
source are distinctly different from those seen in the observations of other
classes. This indicates that the basic accretion disk structure during both dip
and non-dip regions of class is similar, but differ only in intensity.
To explain these observations, we invoke a model in which the viscosity is very
close to critical viscosity and the shock wave is weak or absent.Comment: Replaced with correct figures, Jour. of Astrophysics and Astronomy
(accepted
The viscous disk of GRS 1915+105
GRS 1915+105, one of the two known galactic microquasars, shows an extremely
complex variability in the X-ray band, comparable to no other X-ray source in
the sky. Making use of RXTE/PCA data, we have analyzed the X-ray spectral
distribution throughout the variability. We find that all variations can be
attributed to the rapid appearance and disappearance of the inner region of an
optically-thick accretion disk. Since the time scale for each event is related
to the maximum radius of the disappearing region, the difference in time
structure is due to the time distribution of such radii. The observed relation
between the extent of the missing inner region of the disk and the duration of
an event is in remarkable agreement with the expected radius dependence of the
viscous time scale of the radiation-dominated region of an accretion disk.Comment: 6 pages, 3 figures. To appear in The Active X-ray Sky: Results from
BeppoSAX and Rossi-XTE, Nuclear Physics B Proceedings Supplements, L. Scarsi,
H. Bradt, P. Giommi and F. Fiore (eds.), Elsevier Science B.
Limits on Fast Radio Bursts and other transient sources at 182 MHz using the Murchison Widefield Array
An Integrated TCGA Pan-Cancer Clinical Data Resource to Drive High-Quality Survival Outcome Analytics
For a decade, The Cancer Genome Atlas (TCGA) program collected clinicopathologic annotation data along with multi-platform molecular profiles of more than 11,000 human tumors across 33 different cancer types. TCGA clinical data contain key features representing the democratized nature of the data collection process. To ensure proper use of this large clinical dataset associated with genomic features, we developed a standardized dataset named the TCGA Pan-Cancer Clinical Data Resource (TCGA-CDR), which includes four major clinical outcome endpoints. In addition to detailing major challenges and statistical limitations encountered during the effort of integrating the acquired clinical data, we present a summary that includes endpoint usage recommendations for each cancer type. These TCGA-CDR findings appear to be consistent with cancer genomics studies independent of the TCGA effort and provide opportunities for investigating cancer biology using clinical correlates at an unprecedented scale. Analysis of clinicopathologic annotations for over 11,000 cancer patients in the TCGA program leads to the generation of TCGA Clinical Data Resource, which provides recommendations of clinical outcome endpoint usage for 33 cancer types
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The effect of asymmetries on stock index return value-at-risk estimates
It is widely accepted that equity return volatility increases more following negative shocks rather than positive shocks. However, much of value-at-risk (VaR) analysis relies on the assumption that returns are normally distributed (a symmetric distribution). This article considers the effect of asymmetries on the evaluation and accuracy of VaR by comparing estimates based on various models
Measurement of the polarisation of W bosons produced with large transverse momentum in pp collisions at sqrt(s) = 7 TeV with the ATLAS experiment
This paper describes an analysis of the angular distribution of W->enu and
W->munu decays, using data from pp collisions at sqrt(s) = 7 TeV recorded with
the ATLAS detector at the LHC in 2010, corresponding to an integrated
luminosity of about 35 pb^-1. Using the decay lepton transverse momentum and
the missing transverse energy, the W decay angular distribution projected onto
the transverse plane is obtained and analysed in terms of helicity fractions
f0, fL and fR over two ranges of W transverse momentum (ptw): 35 < ptw < 50 GeV
and ptw > 50 GeV. Good agreement is found with theoretical predictions. For ptw
> 50 GeV, the values of f0 and fL-fR, averaged over charge and lepton flavour,
are measured to be : f0 = 0.127 +/- 0.030 +/- 0.108 and fL-fR = 0.252 +/- 0.017
+/- 0.030, where the first uncertainties are statistical, and the second
include all systematic effects.Comment: 19 pages plus author list (34 pages total), 9 figures, 11 tables,
revised author list, matches European Journal of Physics C versio
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