The Porphyromonas gingivalis hemagglutinins HagB and HagC are major mediators of adhesion and biofilm formation

Porphyromonas gingivalis is a bacterium associated with chronic periodontitis that possesses a family of genes encoding hemagglutinins required for heme acquisition. In this study we generated ΔhagB and ΔhagC mutants in strain W83 and demonstrate that both hagB and hagC are required for adherence to oral epithelial cells. Unexpectedly, a double ΔhagB/ΔhagC mutant had less severe adherence defects than either of the single mutants, but was found to exhibit increased expression of the gingipain-encoding genes rgpA and kgp, suggesting that a ΔhagB/ΔhagC mutant is only viable in populations of cells that exhibit increased expression of genes involved in heme acquisition. Disruption of hagB in the fimbriated strain ATCC33277 demonstrated that HagB is also required for stable attachment of fimbriated bacteria to oral epithelial cells. Mutants of hagC were also found to form defective single and multi-species biofilms that had reduced biomass relative to biofilms formed by the wild-type strain. This study highlights the hitherto unappreciated importance of these genes in oral colonization and biofilm formation

A Hidden Broad-Line Region in the Weak Seyfert 2 Galaxy NGC 788

We have detected a broad H alpha emission line in the polarized flux spectrum of the Seyfert 2 galaxy NGC 788, indicating that it contains an obscured Seyfert 1 nucleus. While such features have been observed in ~15 other Seyfert 2s, this example is unusual because it has a higher fraction of galaxy starlight in its spectrum, a lower average measured polarization, and a significantly lower radio luminosity than other hidden Seyfert 1s discovered to date. This demonstrates that polarized broad-line regions can be detected in relatively weak classical Seyfert 2s, and illustrates why well-defined, reasonably complete spectropolarimetric surveys at H alpha are necessary in order to assess whether or not all Seyfert 2s are obscured Seyfert 1s.Comment: 10 pages using (AASTEX) aaspp4.sty and 4 postscript figures. Publications of the Astronomical Society of the Pacific, Research Notes, in pres

Liquid Transfer Cryogenic Test Facility: Initial hydrogen and nitrogen no-vent fill data

The Liquid Transfer Cryogenic Test Facility is a versatile testbed for ground-based cryogenic fluid storage, handling, and transfer experimentation. The test rig contains two well instrumented tanks, and a third interchangeable tank, designed to accommodate liquid nitrogen or liquid hydrogen testing. The internal tank volumes are approx. 18, 5, and 1.2 cu. ft. Tank pressures can be varied from 2 to 30 psia. Preliminary no vent fill tests with nitrogen and hydrogen were successfully completed with the test rig. Initial results indicate that no vent fills of nitrogen above 90 percent full are achievable using this test configuration, in a 1-g environment, and with inlet liquid temperatures as high as 143 R, and an average tank wall temperature of nearly 300 R. This inlet temperature corresponds to a saturation pressure of 19 psia for nitrogen. Hydrogen proved considerably more difficult to transfer between tanks without venting. The highest temperature conditions resulting in a fill level greater than 90 percent were with an inlet liquid temperature of 34 R, and an estimated tank wall temperature of slightly more than 100 R. Saturation pressure for hydrogen at this inlet temperature is 10 psia. All preliminary no vent fill tests were performed with a top mounted full cone nozzle for liquid injection. The nozzle produces a 120 degree conical droplet spray at a differential pressure of 10 psi. Pressure in the receiving tank was held to less than 30 psia for all tests

BIOMECHANICS OF RUNNING - Electromyographic Analysis of the Hip during Jogging, Running and Sprinting

The patterns of muscle action during human locomotion have been investigated for several decades. Lower extremity electromyographic activity in walking was investigated by Bartholomew and the Prosthetic Research Group at Berkeley in 1953. (1) Others investigated the actions of specific muscles during various leg movements. Basmajian and LeBan et al. (6) examined the electromyographic action of the iliopsoas muscle. These studies have led to the investigation of running. Saito et aI., (10) presented temporal data on the gait cycle of running. James and Brubaker (5) presented a descriptive analysis of running. Other researchers have directed their work toward sprinting: Fenn (4) investigated frictional and kinetic factors in 1930, and Dillman (3) and Ralph Mann (7) used kinetic analysis. Mann and Hagy (9) and Mann et aI. (8) investigated temporal and electromyographic patterns of walking, running and sprinting. This study investigates and describes the electromyographic activity ofthe lower extrJmities and trunk musculature duringjogging, running, and sprinting

Reconciling taxonomy and phylogenetic inference: formalism and algorithms for describing discord and inferring taxonomic roots

Although taxonomy is often used informally to evaluate the results of phylogenetic inference and find the root of phylogenetic trees, algorithmic methods to do so are lacking. In this paper we formalize these procedures and develop algorithms to solve the relevant problems. In particular, we introduce a new algorithm that solves a "subcoloring" problem for expressing the difference between the taxonomy and phylogeny at a given rank. This algorithm improves upon the current best algorithm in terms of asymptotic complexity for the parameter regime of interest; we also describe a branch-and-bound algorithm that saves orders of magnitude in computation on real data sets. We also develop a formalism and an algorithm for rooting phylogenetic trees according to a taxonomy. All of these algorithms are implemented in freely-available software.Comment: Version submitted to Algorithms for Molecular Biology. A number of fixes from previous versio

Investigating diachronic trends in phonological inventories using BDPROTO

Here we present an expanded version of bdproto, a database comprising phonological inventory data from 257 ancient and reconstructed languages. These data were extracted from historical linguistic reconstructions and brought together into a single unified, normalized, accessible, and Unicode-compliant language resource. This dataset is publicly available and we aim to engage language scientists doing research on language change and language evolution. Furthermore, we identify a hitherto undiscussed temporal bias that complicates the simple comparison of ancient and reconstructed languages with present-day languages. Due to the sparsity of the data and the absence of statistical and computational methods that can adequately handle this bias, we instead directly target rates of change within and across families, thereby providing a case study to highlight bdproto’s research viability; using phylogenetic comparative methods and high-resolution language family trees, we investigate whether consonantal and vocalic systems differ in their rates of change over the last 10,000 years. In light of the compilation of bdproto and the findings of our case study, we discuss the challenges involved in comparing the sound systems of reconstructed languages with modern day languages

Very high performance 50 nm T-gate III-V HEMTs enabled by robust nanofabrication technologies

In this paper, we review a range of nanofabrication techniques which enable the realization of uniform, high yield, high performance 50 nm T-gate III-V high electron mobility transistors (HEMTs). These technologies have been applied in the fabrication of a range of lattice matched and pseudomorphic InP HEMTs and GaAs metamorphic HEMTs with functional yields in excess of 95%, threshold voltage uniformity of 5 mV, DC transconductance of up to 1600 mS/mm and f/sub T/ of up to 480 GHz. These technologies and device demonstrators are key to enabling a wide range of millimeter-wave imaging and sensing applications beyond 100 GHz, particularly where array-based multi-channel solutions are required

Statistical analysis of general aviation VG-VGH data

To represent the loads spectra of general aviation aircraft operating in the Continental United States, VG and VGH data collected since 1963 in eight operational categories were processed and analyzed. Adequacy of data sample and current operational categories, and parameter distributions required for valid data extrapolation were studied along with envelopes of equal probability of exceeding the normal load factor (n sub z) versus airspeed for gust and maneuver loads and the probability of exceeding current design maneuver, gust, and landing impact n sub z limits. The significant findings are included

AN ANALYSIS OF THE IMPACT FORCES OF DIFFERENT MODES OF EXERCISE AS A CAUSAL FACTOR TO THE LOW BONE MINERAL DENSITY IN JOCKEYS

The purpose of this study was to investigate the forces placed on the lower limbs of jockeys during riding and to determine whether these were comparable to the impact forces associated with traditional weight bearing activities such as walking and running. Evaluation of these forces will allow isolation of the key causes of the previously reported high incidence of low bone mineral density (BMD) associated with this population and indicate as to whether a lack of weight bearing exercise is a causative factor in this phenomenon. Eight apprentice jockeys completed 6 different activities including walking, running and riding (walk, trot, canter, gallop), where accelerometry data was collected to determine the amount of impact loading applied to the lower limbs. The impact accelerations of the lower limbs in horse riding were significantly lower than those seen in running (