Caracterización molecular de los genes codificantes para las proteínas nitroreductasa tipo i y subunidad b de la calcineurina de Trypanosoma rangeli

Trypanosoma (Herpetosoma) rangeli es un parásito protozoo capaz de infectar hospederos mamíferos sin producir patología en estos. La caracterización biológica y molecular de este parásito se ha enmarcado en el contexto de Trypanosoma cruzi, por cuanto estos parásitos comparten un gran número de características biológicas y presentan una diferencia muy específica en su ciclo de vida: mientras el patógeno T. cruzi puede invadir y replicarse en células del hospedero mamífero, T. rangeli no posee dicha capacidad.Magíster en Ciencias BiológicasMaestrí

Aplicación, Registro y Pago de los Tributos en la Empresa Financiera FUNDENUSE S.A del Municipio de Matagalpa, en el periodo 2016

El presente trabajo de Seminario de Graduación trata de Tributos en las Empresas del departamento de Matagalpa, en el Período 2016, con el objetivo de determinar la Aplicación, Registro y Pago de los Tributos, en la Empresa Financiera FUNDENUSE S.A del municipio de Matagalpa para que se pueda aplicar a las operaciones en las cuales se efectúe el hecho generador del Impuesto en cumplimiento con las leyes y reglamentos estipulados. La importancia de la Implementación de la buena Aplicación, Registro y Pago de los Tributos en la Empresa Financiera FUNDENUSE S.A del Municipio de Matagalpa de conformidad con las leyes fiscales establecidas, para que la empresa no incurra en riesgos fiscales y así pueda establecer mecanismos que le permitan una muy buena administración fiscal. La Empresa Financiera FUNDENUSE S.A del municipio de Matagalpa cuenta con los mecanismos necesarios para el correcto Registro, Aplicación y Pago de los Tributos cumpliendo con las disposiciones que establece la Ley de Concertación Tributaria y demás leyes y reglamentos que rigen el ejercicio fiscal. Por ello la buena implementación de la Aplicación, Registro y Pago de los Tributos le ayudara a la Empresa Financiera FUNDENUSE S.A del municipio de Matagalpa a evitar las sanciones y multas por incumplimiento de la deuda tributaria lo cual le beneficia en sus actividades financieras y evitar cualquier problema que afecte la empres

Identification, characterization and antigenicity of the Plasmodium vivax rhoptry neck protein 1 (PvRON1)

<p>Abstract</p> <p>Background</p> <p><it>Plasmodium vivax </it>malaria remains a major health problem in tropical and sub-tropical regions worldwide. Several rhoptry proteins which are important for interaction with and/or invasion of red blood cells, such as <it>Pf</it>RONs, <it>Pf</it>92, <it>Pf</it>38, <it>Pf</it>12 and <it>Pf</it>34, have been described during the last few years and are being considered as potential anti-malarial vaccine candidates. This study describes the identification and characterization of the <it>P. vivax </it>rhoptry neck protein 1 (<it>Pv</it>RON1) and examine its antigenicity in natural <it>P. vivax </it>infections.</p> <p>Methods</p> <p>The <it>Pv</it>RON1 encoding gene, which is homologous to that encoding the <it>P. falciparum </it>apical sushi protein (ASP) according to the plasmoDB database, was selected as our study target. The <it>pvron1 </it>gene transcription was evaluated by RT-PCR using RNA obtained from the <it>P. vivax </it>VCG-1 strain. Two peptides derived from the deduced <it>P. vivax </it>Sal-I <it>Pv</it>RON1 sequence were synthesized and inoculated in rabbits for obtaining anti-<it>Pv</it>RON1 antibodies which were used to confirm the protein expression in VCG-1 strain schizonts along with its association with detergent-resistant microdomains (DRMs) by Western blot, and its localization by immunofluorescence assays. The antigenicity of the <it>Pv</it>RON1 protein was assessed using human sera from individuals previously exposed to <it>P. vivax </it>malaria by ELISA.</p> <p>Results</p> <p>In the <it>P. vivax </it>VCG-1 strain, RON1 is a 764 amino acid-long protein. <it>In silico </it>analysis has revealed that <it>Pv</it>RON1 shares essential characteristics with different antigens involved in invasion, such as the presence of a secretory signal, a GPI-anchor sequence and a putative sushi domain. The <it>Pv</it>RON1 protein is expressed in parasite's schizont stage, localized in rhoptry necks and it is associated with DRMs. Recombinant protein recognition by human sera indicates that this antigen can trigger an immune response during a natural infection with <it>P. vivax</it>.</p> <p>Conclusions</p> <p>This study shows the identification and characterization of the <it>P. vivax </it>rhoptry neck protein 1 in the VCG-1 strain. Taking into account that <it>Pv</it>RON1 shares several important characteristics with other <it>Plasmodium </it>antigens that play a functional role during RBC invasion and, as shown here, it is antigenic, it could be considered as a good vaccine candidate. Further studies aimed at assessing its immunogenicity and protection-inducing ability in the <it>Aotus </it>monkey model are thus recommended.</p

Evaluación de tres pruebas de pcr para la identificación de Leishmania (Viannia) en diferentes muestras biológicas

Microbiólogo (a) Agrícola y Veterinario (a)Pregrad

Aplicación de metodologías de distribución de plantas para la configuración de un centro de distribución

La distribución en planta se refiere a la organización física de los factores y elementos que participan en el proceso productivo de la empresa y a la determinación de espacios y ubicación de sus distintas secciones. En este caso se pretende1 determinar la distribución más adecuada para el área de almacenamiento en un centro de distribución, teniendo en cuenta las características especiales de los productos que allí se almacenan. Se implementaron la metodología SLP y la heurística de CORELAP para definir la configuración más adecuada de esta sección de acuerdo con las condiciones establecidas en la normatividad

Estilos de vida y riesgo cardiovascular en trabajadores de un laboratorio multinacional de Cali, Colombia

Objective: To describe the beliefs and practices about lifestyles involved in the origin of cardiovascular disease, in workers of a multinational laboratory. Methodology: Descriptive, cross-sectional study, carried out on 96 workers from a multinational laboratory in Cali (Colombia), randomly selected. The Arrivillaga Lifestyle Practices and Beliefs Questionnaire (2005) was used. Results: With a homogeneous sample in terms of gender (50% men and 50% women), an average age of 40.6 years, made up of workers from the operational area (52%) and from the administrative area (43%); hypertension was detected in 10.4% and diabetes mellitus in 2.1% of workers. Although 68.7% of workers answered that they had favorable beliefs, only 38.4% of workers reported having good practices. Conclusions: Although only 10.4% of the sample reported hypertension, this being lower than that calculated nationally (22%), there is a significant percentage of workers at risk (36.8%), which is important to include as an improvement activity in the Cardiovascular Risk Epidemiological Surveillance System.Objetivo: Describir las creencias y las prácticas sobre estilos de vida involucrados en el origen de la enfermedad cardiovascular, en trabajadores de un laboratorio multinacional. Metodología: Estudio descriptivo, de corte transversal, realizado en 96 trabajadores de un laboratorio multinacional en Cali (Colombia), seleccionados aleatoriamente. Se utilizó el Cuestionario de prácticas y creencias sobre Estilo de Vida de Arrivillaga (2005). Resultados: Con una muestra homogénea en términos de género (50% hombres y 50% mujeres), un promedio de edad de 40,6 años, conformada por trabajadores del área operativa (52%) y del área administrativa (43%); se logró detectar hipertensión en el 10,4% y diabetes mellitus en el 2,1% de los trabajadores. Aunque el 68,7% de los trabajadores contestaron tener creencias favorables, solo el 38,4% de los trabajadores refirieron tener buenas prácticas. Conclusiones: Aunque solo el 10,4% de la muestra refirió hipertensión, siendo esto más bajo que lo calculado a nivel nacional (22%), existe porcentaje significativo de trabajadores con riesgo (36,8%), lo cual es importante para incluir como actividad de mejoramiento en el Sistema de Vigilancia Epidemiológica de Riesgo Cardiovascular

Identification, characterization and antigenicity of the Plasmodium vivax rhoptry neck protein 1 (PvRON1)

Background: Plasmodium vivax malaria remains a major health problem in tropical and sub-tropical regions worldwide. Several rhoptry proteins which are important for interaction with and/or invasion of red blood cells, such as PfRONs, Pf92, Pf38, Pf12 and Pf34, have been described during the last few years and are being considered as potential anti-malarial vaccine candidates. This study describes the identification and characterization of the P. vivax rhoptry neck protein 1 (PvRON1) and examine its antigenicity in natural P. vivax infections. Methods: The PvRON1 encoding gene, which is homologous to that encoding the P. falciparum apical sushi protein (ASP) according to the plasmoDB database, was selected as our study target. The pvron1 gene transcription was evaluated by RT-PCR using RNA obtained from the P. vivax VCG-1 strain. Two peptides derived from the deduced P. vivax Sal-I PvRON1 sequence were synthesized and inoculated in rabbits for obtaining anti-PvRON1 antibodies which were used to confirm the protein expression in VCG-1 strain schizonts along with its association with detergent-resistant microdomains (DRMs) by Western blot, and its localization by immunofluorescence assays. The antigenicity of the PvRON1 protein was assessed using human sera from individuals previously exposed to P. vivax malaria by ELISA. Results: In the P. vivax VCG-1 strain, RON1 is a 764 amino acid-long protein. In silico analysis has revealed that PvRON1 shares essential characteristics with different antigens involved in invasion, such as the presence of a secretory signal, a GPI-anchor sequence and a putative sushi domain. The PvRON1 protein is expressed in parasite's schizont stage, localized in rhoptry necks and it is associated with DRMs. Recombinant protein recognition by human sera indicates that this antigen can trigger an immune response during a natural infection with P. vivax. Conclusions: This study shows the identification and characterization of the P. vivax rhoptry neck protein 1 in the VCG-1 strain. Taking into account that PvRON1 shares several important characteristics with other Plasmodium antigens that play a functional role during RBC invasion and, as shown here, it is antigenic, it could be considered as a good vaccine candidate. Further studies aimed at assessing its immunogenicity and protection-inducing ability in the Aotus monkey model are thus recommended