Espécimes de pau-rosa (Aniba rosaeodora Ducke - Lauraceae) na Floresta Nacional ods Tapajós: espécie nativa ameaçada de extinção na Amazônia.

O Pau-Rosa (Aniba rosaeodora Ducke - Lauraceae) é a planta aromática amazônica mais conhecida e importante no comércio de óleos essenciais. Seu óleo é obtido por destilação das folhas, galhos, madeira e raízes, sendo rico em linalol. O produto tem grande demanda no mercado nacional e internacional por ser utilizado na aromaterapia, bem como na perfumaria de alto valor econômico. O corte indiscriminado e manejo inadequado fez com que a espécie fosse incluída pelo IBAMA na Lista Oficial de Espécies da Flora Brasileira Ameaçadas de Extinção. Também é classificada pela Red- List/IUCN na categoria Ameaçada, critério ?em perigo?. Diante disso, o objetivo deste trabalho foi identificar indivíduos na Floresta Nacional do Tapajós como possíveis matrizes de conservação genética nessa Unidade de Conservação e na região. Para identificar os indivíduos existentes realizou-se uma campanha de campo, em 2012, adotaram-se como ponto de referência os quilômetros 67 e 83 da Rodovia BR 163 que liga Santarém a Cuiabá, onde se localizam portões de acesso as áreas estudadas. As atividades obedeceram às normas de pesquisa de campo estabelecidas pelo ICMBIO. Para cada indivíduo identificado por parataxônomo e mateiro obteve-se a localização com auxílio do GPS (Global Positioning System), posteriormente foi elaborado o mapa em Sistema de Informações Geográficas. Foram identificados 15 indivíduos, sendo que 93,3% estavam em ocorrências isoladas

Functional brain asymmetry in motor aphasia during auditory stimulation: report of two cases

This study concerns about brain electrical activity during auditory stimulation in 2 aphasic patients, one with classical (left hemisphere lesion) and another with cross aphasia (right hemisphere lesion). Both cases were submitted to dichotic listening test (consonat-vowel-consonant task) and music audition (gregorian chant), during brain mapping examination. We found, in both cases, a great proportion in delta frequency and power in non-lesional hemisphere during dichotic and musical stimulation. Besides, increasing in frequency of alpha activity was observed only in the non-lesional hemisphere restricted to temporal lobe region. Such findings suggest an interesting field of research about measurements of neurophysiologies correlates of auditory stimulation and brain electrical activity in aphasia.Este estudo diz respeito à atividade elétrica cerebral durante estimulação auditiva em dois pacientes afásicos, um com afasia clássica (lesão no hemisfério esquerdo) e outro com afasia cruzada (lesão no hemisfério direito). Ambos foram submetidos ao teste de estimulação auditiva dicótica (consoante-vogal-consoante) e de audição musical (canto gregoriano) durante mapeamento eletrencefalográfico cerebral. Encontramos, em ambos, grande proporção da frequência delta e potência no hemisfério não lesado, durante a estimulação dicótica e musical. Além disso, foi observado aumento na frequência de atividade alfa somente no hemisfério não lesado, restrito à região do lobo temporal. Esses achados sugerem um interessante campo de pesquisa acerca da medida de correlatos neurofisiológicos de estimulação auditiva e de atividade elétrica cerebral na afasia.Escola Paulista de Medicina Setor de Investigação e Tratamento das EpilepsiasUNIFESP, EPM, Setor de Investigação e Tratamento das EpilepsiasSciEL

First Report of Dieback Caused by Neofusicoccum batangarum in Cashew in Guinea-Bissau

info:eu-repo/semantics/acceptedVersio

FUNCTIONS, FUNCTIONING AND OPTIMIZED POSITIONING OF PRESSURE CONTROL VALVES IN OIL-HYDRAULIC CIRCUITS

The pressure control valves can perform different functions in the hydraulic systems, such as: establish maximum pressure, reduce pressure in some circuit lines, and establish sequence movements, among other functions. The main operation of these valves consists of providing a balance between pressure and the force load on a spring. Most of these valves can be positioned in many different levels, between totally open and totally closed, depending on the flow and on the pressure differential. The pressure control valves are usually named according to their primary functions, e.g., lock wire valve, sequence valve, safety valve, etc. and, their basic function is limit or to determine the pressure of the hydraulic system for the attainment of a certain function of the equipment in motion. They are also classified by the type of connections, by the size and by the selected pressure band. Instead of relief and security, discharge, counterbalance, sequence, reducing and shock suppressor valves represent the pressure control devices. In this paper, oil-hydraulic circuits are suggested for practical lessons of Hydraulic and Pneumatic Commands where they regulate valves of pressure, by acting in the following situations: limiting the maximum pressure of the system, determining a level of pressure, determining two different levels of pressure, determining at the same time two distinct levels of pressure, unloading the pump. The functions of these devices will be discussed and analyzed as an attempt to improve their position in the circuit

Seguimento clínico de dois pacientes brasileiros com MODY-glicoquinase (MODY2) e descrição de uma nova mutação

Mutations in the glucokinase gene (GCK) account for many cases of monogenic diabetes featuring maturity-onset diabetes of the young type 2 (MODY2). The clinical pattern of this form of hyperglycemia is rather stable, with a slight elevation in blood glucose, which is usually not progressive. Patients rarely require pharmacological interventions and microvascular complications related to diabetes are unusual. We describe the clinical follow-up of two cases of MODY2 with two different mutations in GCK gene, one in exon 7, p.Glu265Lys (c.793 G> A), which has been previously described, and a novel one, in exon 2, p.Ser69Stop (c. 206C> G). The clinical course of both cases shows similarity in metabolic control of this form of diabetes over the years. Arq Bras Endocrinol Metab. 2012;56(8):490-5Mutações no gene da glicoquinase (GCK) são determinantes de uma forma de diabetes monogênico denominada de MODY2 (maturity-onset diabetes of the young, tipo 2). O padrão clínico dessa forma de distúrbio glicêmico é bastante estável, com hiperglicemia leve, geralmente não progressiva. Intervenções farmacológicas raramente são necessárias e complicações crônicas secundárias ao diabetes são infrequentes. Descrevemos o acompanhamento clínico de dois casos de MODY2 com duas mutações diferentes, uma no éxon 7, p.Glu265Lys (c.793 G>A) já descrita anteriormente, e outra inédita no éxon 2 p.Ser69Stop (c. 206C>G). A evolução clínica de ambos os casos demonstra uma semelhança no padrão metabólico dessa forma de diabetes ao longo dos anos. Arq Bras Endocrinol Metab. 2012;56(8):490-5Faculdade de Medicina da Universidade de São Paulo Hospital das Clínicas Instituto da CriançaUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Laboratório de Endocrinologia Molecular e TranslacionalUNIFESP-EPM Centro de DiabetesUNIFESP, EPM, Laboratório de Endocrinologia Molecular e TranslacionalUNIFESP, EPM Centro de DiabetesSciEL

Genetic polymorphisms and coronary artery disease in the portuguese population: the GENEMACOR Study

PosterMultiple studies have showed an association between genetic polymorphisms and the risk of coronary artery disease (CAD). Initially, studies focused mainly in variants acting in pathophysiological axis of CAD or its risk factors. Genome-Wide Association Studies (GWAS) revealed other genes that, besides having an unknown mechanism, are statistically significant. The importance of these in the development of CAD in the Portuguese population is unknown. Objective: Analyze the genetic polymorphisms associated with the development of CAD in a Portuguese population. Methods: We performed a case-control study with 1321 consecutive coronary patients (mean age 53.4 ± 8.1 years, 78.8% male) and 1148 controls (adjusted for age and sex) selected from GENEMACOR Study, an ongoing study designed to analyze the genetic profile of a Portuguese population. We evaluated, in both groups, 29 genetic variants previously associated with CAD: ACE I/D, AGT235 M/T, ATIR A/C, MTHFR C/T and 1298 A/C, PON192 Q/R and 55 L/M,LPA T/C, APO E, Locus 9p21.3 (rs1333049), CDKN2B (rs4977574), GJA4 C/T, PCSK9 A/G, TAS2R50 A/G, KIF6 C/T, IGF2BP2 G/T, ADAMTS7 A/G, MC4R T/C, PPARG Pro12 Ala, ZNF259 C/G, SMAD3 C/T, MIA3 C/A, MTHFD1L A/G, SLC30A8 C/T, TCF7L2 C/T, HNF4 C/G, FTO A/C and ADIPOQ C/G. Allele and genotypic frequencies of individuals with and without CAD were compared and the strength of association was expressed by the OR as well as by CI 95%. Results: The variants rs4340 (ACE I/D), rs266729 (ADIPOQ C/G), rs458560 (PON55 L/M), rs429358 (APOE2), LPA T/C, rs1333049 (locus 9p21.3) and rs4977574 (CDKN2B A/G) were significantly associated with CAD (p<0.05) (Table). Conclusions: In our population, the genetic polymorphisms significantly related to CAD were: ACE, associated with hypertension; ADIPOQ, associated with obesity; PON55, associated with oxidation; APOE and LPA, associated with dyslipidemia and finally the locus 9p21.3 with a unclear mechanism so far.info:eu-repo/semantics/publishedVersio

Influence of PLLA/PCL/HA scaffold fiber orientation on mechanical properties and osteoblast behavior

Scaffolds based on aligned and non-aligned poly (L-lactic acid) (PLLA)/polycaprolactone (PCL) fibers obtained by electrospinning, associated to electrosprayed hydroxyapatite (HA) for tissue engineering applications were developed and their performance was compared in terms of their morphology and biological and mechanical behaviors. The morphological results assessed by scanning electron microscopy showed a mesh of PLLA/PCL fibers (random and perfectly aligned) associated with aggregates of nanophased HA. Fourier transform infrared spectrometry confirmed the homogeneity in the blends and the presence of nanoHA in the scaffold. As a result of fiber alignment a 15-fold increase in Young’s Modulus and an 8-fold increase in tensile strength were observed when compared to non-aligned fibers. In PLLA/PCL/HA scaffolds, the introduction of nanoHA caused a remarkable improvement of the mechanical strength of this material acting as a reinforcement, enhancing the response of these constructs to tensile stress. In vitro testing was evaluated using osteoblast (MC3T3-E1) cells. The results showed that both fibrous scaffolds were able to support osteoblast cell adhesion and proliferation and that fiber alignment induced increased cellular metabolic activity. In addition, the adhesion and proliferation of Staphylococcus aureus were evaluated and a lower number of colony forming units (CFUs) was obtained in the scaffolds with aligned fibers.info:eu-repo/semantics/publishedVersio