Human colon cancer cell lines show a diverse pattern of nitric oxide synthase gene expression and nitric oxide generation.

A panel of human colonic adenocarcinoma cell lines was examined both for expression of mRNAs of the nitric oxide synthase (NOS) gene family and for evidence of enzymic activity based on citrulline and nitrite (NO2-) formation. Reverse transcription-polymerase chain reaction (RT-PCR), revealed that all lines (SW480, SW620, DLD-1 and WiDr) expressed mRNA for the Ca(2+)-dependent endothelial (e)NOS, while SW480 cells also expressed the Ca(2+)-dependent neuronal (n)NOS. The mRNA for the Ca(2+)-independent inducible (i)NOS was expressed both by cytokine-stimulated and by unstimulated SW480, SW620 and DLD-1 cells, but none was seen at any time in the WiDr cells. There was, however, little correlation between mRNA expression and enzymic activity based on citrulline and NO2- formation. Thus none of the cell lines exhibited measurable Ca(2+)-dependent NOS activity, while Ca(2+)-independent NOS activity was seen in all but the WiDr cells. Furthermore, DLD-1 cells generated citrulline with resultant NO2- formation only after stimulation with lipopolysaccharide (LPS) and/or cytokines, while SW480 and SW620 did so constitutively. Thus RT-PCR studies indicate that tumour cells of similar epithelial origin display a diverse pattern of NOS gene family expression, and parallel biochemical studies clearly indicate that such expression does not always result in measurable enzymic activity leading to the generation of NO

Cryptosporidium y blastocistis hominis como agentes patógenos en el síndrome diarréico

En una comunidad de escasos recursos de Bogotá se tomaron muestras de heces de niños menores de diez años con diarrea y de niños sin diarrea. Las muestras del grupo de estudio y del grupo control resultaron negativas para el Cryptosporidium. Se encontraron positivas para Blastocistis hominis 17 muestras del grupo de estudio (8.3%). y 5 del grupo control (10.4%). Los síntomas predominantes fueron fiebre, dolor abdominal y pérdida del apetito. El B. hominis se asoció con la Escherichia coli, Salmonella campylobacter, E. histiolytica, Giardia lamblia, Ascaris lumbricoides y rotavírus. No se confirmó el papel que en los últimos años se le atribuye al Cryptosporidium y al B. hominis como agentes productores de diarrea

Hydrogymnastics training program on physical fitness in elderly women

Se evaluó un programa de entrenamiento de hidrogimnasia sobre las capacidades físicas en 26 adultas mayores (grupo experimental n=16, grupo control n=10), determinadas mediante el protocolo senior fitness test, la hidrogimnasia fue 5 veces por semana por 12 semanas, con ejercicios aeróbicos del 50%-60% de la FCmáx. Se utilizó ANOVA 2x2, indicando interacción significativa (p=0.052) entre grupos y mediciones en la prueba de resistencia a la fuerza en extremidades inferiores, la prueba de agilidad de 2.4 metros tuvo interacción significativa (p≤0.01) entre grupos y mediciones, las pruebas de capacidad aeróbica step test de 2 minutos (p=0.02) y caminar 6 minutos indicaron interacción significativa entre grupos y mediciones (p=0.50); Se infiere que la hidrogimnasia en las adultas mayores participantes es eficaz en la mejora de las capacidades físicas principalmente aeróbicas, de resistencia a la fuerza y agilidad de miembros inferioresThe aim of this study was to evaluate the effect of a hydrogymnastics training program on physical fitness in 26 elderly women (experimental group n=16, control group n=10). The physical fitness was determined by the senior fitness test protocol, the hydrogymnastics consisted in aerobic exercise at 50%-60% of maximum heart rate, 5 times a week in a period of 12 weeks. ANOVA 2x2 as statistical test was used, indicating statistically significant interaction (p=0.052) between groups and measurements on test strength endurance in the legs by squatting on chair in 30 s, agility test 2.4 meters was significant interaction (p≤0.01) between groups and measures, testing aerobic capacity test step 2 minutes (p=0.02) and six minutes walk indicated significant interaction between groups and measurements (p=0.50), the results shown that hydrogymnastics in the elderly women participants was effective improving endurance, strength and agility mainly in leg

Inhibition of the sodium-dependent HCO₃ - transporter SLC4A4, produces a cystic fibrosis-like airway disease phenotype

\ua9 2022, eLife Sciences Publications Ltd. All rights reserved. Bicarbonate secretion is a fundamental process involved in maintaining acid-base homeostasis. Disruption of bicarbonate entry into airway lumen, as has been observed in cystic fibrosis, produces several defects in lung function due to thick mucus accumulation. Bicarbonate is critical for correct mucin deployment and there is increasing interest in understanding its role in airway physiology, particularly in the initiation of lung disease in children affected by cystic fibrosis, in the absence of detectable bacterial infection. The current model of anion secretion in mammalian airways consists of CFTR and TMEM16A as apical anion exit channels, with limited capacity for bicarbonate transport compared to chloride. However, both channels can couple to SLC26A4 anion exchanger to maximise bicarbonate secretion. Nevertheless, current models lack any details about the identity of the basolateral protein(s) responsible for bicarbonate uptake into airway epithelial cells. We report herein that the electrogenic, sodium-dependent, bicarbonate cotransporter, SLC4A4, is expressed in the basolateral membrane of human and mouse airways, and that it’s pharmacological inhibition or genetic silencing reduces bicarbonate secretion. In fully differentiated primary human airway cells cultures, SLC4A4 inhibition induced an acidification of the airways surface liquid and markedly reduced the capacity of cells to recover from an acid load. Studies in the Slc4a4-null mice revealed a previously unreported lung phenotype, characterized by mucus accumulation and reduced mucociliary clearance. Collectively, our results demonstrate that the reduction of SLC4A4 function induced a CF-like phenotype, even when chloride secretion remained intact, highlighting the important role SLC4A4 plays in bicarbonate secretion and mammalian airway function

Small is fast: astrocytic glucose and lactate metabolism at cellular resolution

Brain tissue is highly dynamic in terms of electrical activity and energy demand. Relevant energy metabolites have turnover times ranging from milliseconds to seconds and are rapidly exchanged between cells and within cells. Until recently these fast metabolic events were inaccessible, because standard isotopic techniques require use of populations of cells and/or involve integration times of tens of minutes. Thanks to fluorescent probes and recently available genetically-encoded optical nanosensors, this Technology Report shows how it is now possible to monitor the concentration of metabolites in real-time and in single cells. In combination with ad hoc inhibitor-stop protocols, these probes have revealed a key role for K(+) in the acute stimulation of astrocytic glycolysis by synaptic activity. They have also permitted detection of the Warburg effect in single cancer cells. Genetically-encoded nanosensors currently exist for glucose, lactate, NADH and ATP, and it is envisaged that other metabolite nanosensors will soon be available. These optical tools together with improved expression systems and in vivo imaging, herald an exciting era of single-cell metabolic analysis

Tratamiento con octreotide (SMS 201-995) en un caso de tumor carcinoide intestinal

Over the past few years the usefulness of some somatostatin's analogues in the treatment of intestinal tract endocrine tumors has been demonstrated. Notwithstanding, the results obtained are variable. The case of a carcinoid tumor with a hepatic metastasis is presented and its clinical as well as its biochemical and its morphological results are evaluated after treatment with octreotide over a seven months period. It is important to highlight the great clinical improvement obtained at the beginning of treatment. Treatment was not effective in the control of tumor progression. After the injection of such a drug, a decrease in serotonin and 5-hydroxy-indoleacetic acid serum levels was observed as well as a reduction in the urinary metabolite. It is concluded that octreotide is very useful for the symptomatic treatment of carcinoid syndrome

Regulation of neutrophil senescence by microRNAs

Neutrophils are rapidly recruited to sites of tissue injury or infection, where they protect against invading pathogens. Neutrophil functions are limited by a process of neutrophil senescence, which renders the cells unable to respond to chemoattractants, carry out respiratory burst, or degranulate. In parallel, aged neutrophils also undergo spontaneous apoptosis, which can be delayed by factors such as GMCSF. This is then followed by their subsequent removal by phagocytic cells such as macrophages, thereby preventing unwanted inflammation and tissue damage. Neutrophils translate mRNA to make new proteins that are important in maintaining functional longevity. We therefore hypothesised that neutrophil functions and lifespan might be regulated by microRNAs expressed within human neutrophils. Total RNA from highly purified neutrophils was prepared and subjected to microarray analysis using the Agilent human miRNA microarray V3. We found human neutrophils expressed a selected repertoire of 148 microRNAs and that 6 of these were significantly upregulated after a period of 4 hours in culture, at a time when the contribution of apoptosis is negligible. A list of predicted targets for these 6 microRNAs was generated from http://mirecords.biolead.org and compared to mRNA species downregulated over time, revealing 83 genes targeted by at least 2 out of the 6 regulated microRNAs. Pathway analysis of genes containing binding sites for these microRNAs identified the following pathways: chemokine and cytokine signalling, Ras pathway, and regulation of the actin cytoskeleton. Our data suggest that microRNAs may play a role in the regulation of neutrophil senescence and further suggest that manipulation of microRNAs might represent an area of future therapeutic interest for the treatment of inflammatory disease

Visual Evaluation of the Soil Structure under Different Management Systems in Lowlands in Southern Brazil.

ABSTRACT The “Visual Evaluation of Soil Structure” (VESS) is a method used primarily to evaluate the soil structural quality of Oxisols in Brazil and secondly for more specific research, consultancy, and teaching purposes. Since the methodology was never applied and compared with laboratory evaluations of physical properties of hydromorphic soils of the Pampa biome in the south of Brazil, this study evaluated the use of VESS as a visual indicator of the structure quality of a typic eutrophic Albaqualf soil under native grassland, crop-livestock integration, no-tillage, and conventional management systems. Experimental areas with these different management systems were subjected to visual (VESS) and laboratory evaluation of the soil structure. The laboratory evaluation was based on traditional methods and on measurements of bulk density, porosity, aggregate mean weight diameter, aggregate tensile strength (ATS), and total organic carbon (TOC). It was concluded that VESS was efficient in differentiating the management system. The management systems based on minimum soil disturbance and mulching with crop residues improved the soil quality, as evidenced by the VESS scores, bulk density, porosity, aggregation, and organic carbon. The TOC content was inversely related with ATS. The quality of a typic eutrophic Albaqualf was benefitted by organic matter in the surface layer