An Exploration of the Occupational Therapy Doctoral Capstone: Perspectives from Capstone Coordinators, Graduates, and Site Mentors

Few studies have investigated entry-level doctoral capstone outcomes regarding how the experience and project relate to employment opportunities. This descriptive study reports various stakeholders’ perspectives regarding the entry-level occupational therapy doctoral (OTD) capstone in relation to employment opportunities and awareness of the profession. Authors disseminated a nationwide survey to doctoral capstone coordinators, entry-level OTD graduates, and capstone site mentors. Ten doctoral capstone coordinators, 68 OTD graduates, and 22 capstone site mentors responded to the survey. Collectively, doctoral capstone coordinators reported that 117 OTD graduates obtained employment in a setting related to the focus of their doctoral capstone and gave examples of graduates employed as entrepreneurs, faculty, program specialists, and supervisors. OTD graduates most often completed their doctoral capstones in community-based settings with projects focusing on program development. Sixty-four percent of the OTD graduates were employed at their doctoral capstone site or at a site similar to where they completed their doctoral capstone. Professional background of capstone site mentors varied, and all mentors who were not occupational therapy professionals reported improved awareness of occupational therapy after mentoring an OTD student. Outcomes of the OTD doctoral capstone are needed to support the efficacy of this experience and project. This study begins to provide such outcomes by reporting findings related to employment and awareness of the profession

Ram locus is a key regulator to trigger multidrug resistance in Enterobacter aerogenes

Purpose: Several genetic regulators belonging to AraC family are involved in the emergence of MDR isolates of E. aerogenes due to alterations in membrane permeability. Compared with the genetic regulator Mar, RamA may be more relevant towards the emergence of antibiotic resistance. Methodology: Focusing on the global regulators, Mar and Ram, we compared the amino acid sequences of the Ram repressor in 59 clinical isolates and laboratory strains of E. aerogenes. Sequence types were associated with their corresponding multi-drug resistance phenotypes and membrane protein expression profiles using MIC and immunoblot assays. Quantitative gene expression analysis of the different regulators and their targets (porins and efflux pump components) were performed. Results: In the majority of the MDR isolates tested, ramR and a region upstream of ramA were mutated but marR or marA were unchanged. Expression and cloning experiments highlighted the involvement of the ram locus in the modification of membrane permeability. Overexpression of RamA lead to decreased porin production and increased expression of efflux pump components, whereas overexpression of RamR had the opposite effects. Conclusion: Mutations or deletions in ramR, leading to the overexpression of RamA predominated in clinical MDR E. aerogenes isolates and were associated with a higher-level of expression of efflux pump components. It was hypothesised that mutations in ramR, and the self-regulating region proximal to ramA, probably altered the binding properties of the RamR repressor; thereby producing the MDR phenotype. Consequently, mutability of RamR may play a key role in predisposing E. aerogenes towards the emergence of a MDR phenotype

A Snapshot of How Entry-Level Occupational Therapy Programs Collect and Use Doctoral Capstone Outcomes

Entry-level occupational therapy doctorate (OTD) programs are required to gather program evaluation data regarding the doctoral capstone for accreditation purposes. This study aims to describe the processes used by a sample of OTD programs to gather doctoral capstone outcome data and to determine the type of data collected in order to understand the perspectives regarding doctoral capstone outcomes by doctoral capstone coordinators (DCCs) within the United States. The investigators used purposeful sampling to recruit DCCs at accredited entry-level OTD programs in the United States. The most common outcome data collected included information about the doctoral capstone experience from students, student performance outcomes, employment and scholarship outcomes from alumni and employers, and various outcomes from site/expert/faculty mentors. Results highlight the need to disseminate doctoral capstone outcomes as few participants in this study used the data they were collecting outside of their program. By sharing and reporting outcomes of the doctoral capstone, OTD programs, and the profession can collectively benefit. Development of common outcomes, collected across programs, can help to provide data large enough to draw meaningful conclusions regarding the value of the doctoral capstone in occupational therapy education and in strengthening the knowledge of the profession among the public

Gain-of-function screen for genes that affect Drosophila muscle pattern formation.

This article reports the production of an EP-element insertion library with more than 3,700 unique target sites within the Drosophila melanogaster genome and its use to systematically identify genes that affect embryonic muscle pattern formation. We designed a UAS/GAL4 system to drive GAL4-responsive expression of the EP-targeted genes in developing apodeme cells to which migrating myotubes finally attach and in an intrasegmental pattern of cells that serve myotubes as a migration substrate on their way towards the apodemes. The results suggest that misexpression of more than 1.5% of the Drosophila genes can interfere with proper myotube guidance and/or muscle attachment. In addition to factors already known to participate in these processes, we identified a number of enzymes that participate in the synthesis or modification of protein carbohydrate side chains and in Ubiquitin modifications and/or the Ubiquitin-dependent degradation of proteins, suggesting that these processes are relevant for muscle pattern formation

Molecular and functional characterisation of the Arabidopsis PRC1 complex

Les protéines du groupe Polycomb sont des régulateurs épigénétiques impliqués dans divers processus développementaux et cellulaires. Le complexe Polycomb Répressif 1 (PRC1) est bien caractérisé chez les animaux, cependant sa composition et sa fonction restent énigmatiques dans les plantes. Sur base d'homologie de séquences trois homologues de la sous-unité de base BMI1 du complexe PRC1 animal ont été identifiés dans Arabidopsis: AtBMI1a, AtBMI1b et AtBMI1c. L'interaction de ces trois protéines avec les composantes PRC1 connues (i.e. AtRING1ab, et LHP1) a été démontrée. Des analyses génétiques et moléculaires ont permis d'attribuer aux protéines AtBMI1ab et AtRING1ab un rôle essentiel dans la répression des caractères embryonnaire lors de la croissance végétative. Un nouvel interactant d'AtRING1a, une protéine à domaine PHD de la famille AL (Alfine-Like) a été identifiée dans criblage d'une banque de ADNc. Par différentes techniques l'association entre les protéines de la famille AL et les membres de bases du complexe PRC1 (i.e. AtBMI1ab, AtRING1ab et LHP1) a été démontrée. Les protéines AL sont nucléaires et se lient in vitro à H3k4me3, une marque active de la chromatine. Des analyses génétiques ont révélé que les protéines AL et AtBMI1ab régulent la germination en réprimant l'expression de gènes impliqués dans le développement de la graine. Au niveau chromatinien, les protéines PRC1 interviennent dans la transition d'une chromatine active, marquée par du H3K4me3 vers une chromatine répressive enrichie en H3K27me3. Nous proposons que les protéines AL reconnaissent la marque active et recrutent la fonction répressive des protéines à domaine RING du complexe PRC1 afin d'induire la répression transcriptionelle.Polycomb group (PcG) proteins are critical epigenetic repressors implicated in various developmental and cellular processes. While the Polycomb Repressive Complex 2 (PRC2) is evolutionary conserved and its functions extensively studied in Arabidopsis, the PRC1 complex composition and function remain still enigmatic in plants. Our work focuses on several Arabidopsis RING-domain proteins to unravel PRC1-like functions in the regulation of various processes during plant development. Based on sequence similarity we identified three homologues of the animal PRC1 core subunit BMI1: AtBMI1a, AtBMI1b and AtBMI1c. These proteins were found to interact with other PRC1-like components, AtRING1a, AtRING1b and LHP1. Genetic and molecular analyses demonstrated that AtBMI1a/b and AtRING1a/b play crucial roles in stable repression of embryonic traits to allow proper somatic growth. Comparative transcriptome analyses were performed to uncover genetic networks underlying seedling growth and the flower development defects of several different PRC1-like and PRC2 Arabidopsis mutants. Our data revealed overlapping and non-overlapping gene categories of misregulated genes in Atring1a/b, Atbmi1a/b and lhp1 mutants. The Atring1a/b mutant showed particular disturbed expression of flower developmental genes. Accordingly, phenotypic and molecular analyses of the mutant flowers confirmed that AtRING1a/b play a critical role in cell fate determination and in different aspects of flower development. To better understand the broad function of AtRING1a/b, we performed yeast two-hybrid screen and identified PHD-domain proteins of the ALFIN-LIKE (AL) family as binding partners. In vitro AL proteins bind the active mark for gene transcription, H3K4me3. By various methods, both in vitro and in planta, we provided strong evidence for the physical interaction between AL and PRC1 RING-domain proteins. We uncovered that al6/7 similar to Atbmi1a/b mutants exhibit seed germination defects, which are associated with the derepression of several seed related genes. Consistently on the corresponding chromatin a delay of the remodeling from active H3K4me3 labeled to a repressive H3K27me3 marked chromatin could be detected. We propose that through binding to H3K4me3 AL6/7 function as scaffold proteins to target PRC1 RING-domain proteins to active chromatin in order to establish gene silencing. Taken together, the presented work contributes significantly to the knowledge of PRC1 complex(es) in Arabidopsis at both biological function and complex composition levels. It opens several exciting perspectives for future research in the field

Beteiligung von Lernenden an der Unterrichtsgestaltung am Lycée Ermesinde

Partizipativer Unterricht, der nicht nur aus punktuell erlebter Teilnahme besteht, ist durch die Herausforderungen, vor die uns unsere globalisierte Welt stellt, in vielerlei Hinsicht unabdingbar geworden. Kommunikationsfähigkeit, interkulturelle Kompetenz, Wissensmanagement und andere oft zitierte „soft skills“ sind Schlüsselkompetenzen des 21. Jahrhunderts. (DIPF/Orig.

Molecular and functional characterisation of the Arabidopsis PRC1 complex

Les protéines du groupe Polycomb sont des régulateurs épigénétiques impliqués dans divers processus développementaux et cellulaires. Le complexe Polycomb Répressif 1 (PRC1) est bien caractérisé chez les animaux, cependant sa composition et sa fonction restent énigmatiques dans les plantes. Sur base d'homologie de séquences trois homologues de la sous-unité de base BMI1 du complexe PRC1 animal ont été identifiés dans Arabidopsis: AtBMI1a, AtBMI1b et AtBMI1c. L'interaction de ces trois protéines avec les composantes PRC1 connues (i.e. AtRING1ab, et LHP1) a été démontrée. Des analyses génétiques et moléculaires ont permis d'attribuer aux protéines AtBMI1ab et AtRING1ab un rôle essentiel dans la répression des caractères embryonnaire lors de la croissance végétative. Un nouvel interactant d'AtRING1a, une protéine à domaine PHD de la famille AL (Alfine-Like) a été identifiée dans criblage d'une banque de ADNc. Par différentes techniques l'association entre les protéines de la famille AL et les membres de bases du complexe PRC1 (i.e. AtBMI1ab, AtRING1ab et LHP1) a été démontrée. Les protéines AL sont nucléaires et se lient in vitro à H3k4me3, une marque active de la chromatine. Des analyses génétiques ont révélé que les protéines AL et AtBMI1ab régulent la germination en réprimant l'expression de gènes impliqués dans le développement de la graine. Au niveau chromatinien, les protéines PRC1 interviennent dans la transition d'une chromatine active, marquée par du H3K4me3 vers une chromatine répressive enrichie en H3K27me3. Nous proposons que les protéines AL reconnaissent la marque active et recrutent la fonction répressive des protéines à domaine RING du complexe PRC1 afin d'induire la répression transcriptionelle.Polycomb group (PcG) proteins are critical epigenetic repressors implicated in various developmental and cellular processes. While the Polycomb Repressive Complex 2 (PRC2) is evolutionary conserved and its functions extensively studied in Arabidopsis, the PRC1 complex composition and function remain still enigmatic in plants. Our work focuses on several Arabidopsis RING-domain proteins to unravel PRC1-like functions in the regulation of various processes during plant development. Based on sequence similarity we identified three homologues of the animal PRC1 core subunit BMI1: AtBMI1a, AtBMI1b and AtBMI1c. These proteins were found to interact with other PRC1-like components, AtRING1a, AtRING1b and LHP1. Genetic and molecular analyses demonstrated that AtBMI1a/b and AtRING1a/b play crucial roles in stable repression of embryonic traits to allow proper somatic growth. Comparative transcriptome analyses were performed to uncover genetic networks underlying seedling growth and the flower development defects of several different PRC1-like and PRC2 Arabidopsis mutants. Our data revealed overlapping and non-overlapping gene categories of misregulated genes in Atring1a/b, Atbmi1a/b and lhp1 mutants. The Atring1a/b mutant showed particular disturbed expression of flower developmental genes. Accordingly, phenotypic and molecular analyses of the mutant flowers confirmed that AtRING1a/b play a critical role in cell fate determination and in different aspects of flower development. To better understand the broad function of AtRING1a/b, we performed yeast two-hybrid screen and identified PHD-domain proteins of the ALFIN-LIKE (AL) family as binding partners. In vitro AL proteins bind the active mark for gene transcription, H3K4me3. By various methods, both in vitro and in planta, we provided strong evidence for the physical interaction between AL and PRC1 RING-domain proteins. We uncovered that al6/7 similar to Atbmi1a/b mutants exhibit seed germination defects, which are associated with the derepression of several seed related genes. Consistently on the corresponding chromatin a delay of the remodeling from active H3K4me3 labeled to a repressive H3K27me3 marked chromatin could be detected. We propose that through binding to H3K4me3 AL6/7 function as scaffold proteins to target PRC1 RING-domain proteins to active chromatin in order to establish gene silencing. Taken together, the presented work contributes significantly to the knowledge of PRC1 complex(es) in Arabidopsis at both biological function and complex composition levels. It opens several exciting perspectives for future research in the field

A Portrait of Four in Glass: The Costume Design for The Glass Menagerie

AN ABSTRACT OF THE THESIS OF Stephanie Anne Molitor, for the Master of Fine Arts degree in Theater, presented on 2 July 2010, at Southern Illinois University Carbondale. TITLE: A PORTRAIT OF FOUR IN GLASS: THE COSTUME DESIGN FOR THE GLASS MENAGERIE MAJOR PROFESSOR: Wendi Zea The Department of Theater at Southern Illinois University Carbondale presented Tennessee Williams\u27 The Glass Menagerie in October 2011. This thesis presents the documentation and analysis of my costume design process for this piece. Chapter 1 contains an analysis of the script, including pertinent biographical information about the playwright, which relates to the play. Chapter 2 presents documentation on my design and collaboration process, while Chapter 3 notes the process of building and presenting the show. Chapter 4 is an evaluation, which assesses my strengths and weaknesses throughout the production, as well as an analysis of the feedback I received. The appendices contain process sketches, construction and execution paperwork, renderings, and production photos

Molecular and functional characterisation of the Arabidopsis PRC1 complex

Les protéines du groupe Polycomb sont des régulateurs épigénétiques impliqués dans divers processus développementaux et cellulaires. Le complexe Polycomb Répressif 1 (PRC1) est bien caractérisé chez les animaux, cependant sa composition et sa fonction restent énigmatiques dans les plantes. Sur base d'homologie de séquences trois homologues de la sous-unité de base BMI1 du complexe PRC1 animal ont été identifiés dans Arabidopsis: AtBMI1a, AtBMI1b et AtBMI1c. L'interaction de ces trois protéines avec les composantes PRC1 connues (i.e. AtRING1ab, et LHP1) a été démontrée. Des analyses génétiques et moléculaires ont permis d'attribuer aux protéines AtBMI1ab et AtRING1ab un rôle essentiel dans la répression des caractères embryonnaire lors de la croissance végétative. Un nouvel interactant d'AtRING1a, une protéine à domaine PHD de la famille AL (Alfine-Like) a été identifiée dans criblage d'une banque de ADNc. Par différentes techniques l'association entre les protéines de la famille AL et les membres de bases du complexe PRC1 (i.e. AtBMI1ab, AtRING1ab et LHP1) a été démontrée. Les protéines AL sont nucléaires et se lient in vitro à H3k4me3, une marque active de la chromatine. Des analyses génétiques ont révélé que les protéines AL et AtBMI1ab régulent la germination en réprimant l'expression de gènes impliqués dans le développement de la graine. Au niveau chromatinien, les protéines PRC1 interviennent dans la transition d'une chromatine active, marquée par du H3K4me3 vers une chromatine répressive enrichie en H3K27me3. Nous proposons que les protéines AL reconnaissent la marque active et recrutent la fonction répressive des protéines à domaine RING du complexe PRC1 afin d'induire la répression transcriptionelle.Polycomb group (PcG) proteins are critical epigenetic repressors implicated in various developmental and cellular processes. While the Polycomb Repressive Complex 2 (PRC2) is evolutionary conserved and its functions extensively studied in Arabidopsis, the PRC1 complex composition and function remain still enigmatic in plants. Our work focuses on several Arabidopsis RING-domain proteins to unravel PRC1-like functions in the regulation of various processes during plant development. Based on sequence similarity we identified three homologues of the animal PRC1 core subunit BMI1: AtBMI1a, AtBMI1b and AtBMI1c. These proteins were found to interact with other PRC1-like components, AtRING1a, AtRING1b and LHP1. Genetic and molecular analyses demonstrated that AtBMI1a/b and AtRING1a/b play crucial roles in stable repression of embryonic traits to allow proper somatic growth. Comparative transcriptome analyses were performed to uncover genetic networks underlying seedling growth and the flower development defects of several different PRC1-like and PRC2 Arabidopsis mutants. Our data revealed overlapping and non-overlapping gene categories of misregulated genes in Atring1a/b, Atbmi1a/b and lhp1 mutants. The Atring1a/b mutant showed particular disturbed expression of flower developmental genes. Accordingly, phenotypic and molecular analyses of the mutant flowers confirmed that AtRING1a/b play a critical role in cell fate determination and in different aspects of flower development. To better understand the broad function of AtRING1a/b, we performed yeast two-hybrid screen and identified PHD-domain proteins of the ALFIN-LIKE (AL) family as binding partners. In vitro AL proteins bind the active mark for gene transcription, H3K4me3. By various methods, both in vitro and in planta, we provided strong evidence for the physical interaction between AL and PRC1 RING-domain proteins. We uncovered that al6/7 similar to Atbmi1a/b mutants exhibit seed germination defects, which are associated with the derepression of several seed related genes. Consistently on the corresponding chromatin a delay of the remodeling from active H3K4me3 labeled to a repressive H3K27me3 marked chromatin could be detected. We propose that through binding to H3K4me3 AL6/7 function as scaffold proteins to target PRC1 RING-domain proteins to active chromatin in order to establish gene silencing. Taken together, the presented work contributes significantly to the knowledge of PRC1 complex(es) in Arabidopsis at both biological function and complex composition levels. It opens several exciting perspectives for future research in the field.STRASBOURG-Bib.electronique 063 (674829902) / SudocSudocFranceF