972 research outputs found
Transcriptomic analysis of the hepatic response to stress in the red cusk-eel (Genypterus chilensis): Insights into lipid metabolism, oxidative stress and liver steatosis
Indexación: Scopus.Teleosts exhibit a broad divergence in their adaptive response to stress, depending on the magnitude, duration, and frequency of stressors and the species receiving the stimulus. We have previously reported that the red cusk-eel (Genypterus chilensis), an important marine farmed fish, shows a physiological response to stress that results in increased skeletal muscle atrophy mediated by over-expression of components of the ubiquitin proteasome and autophagy-lysosomal systems. To better understand the systemic effects of stress on the red cusk-eel metabolism, the present study assessed the transcriptomic hepatic response to repetitive handling-stress. Using high-Throughput RNA-seq, 259 up-regulated transcripts were found, mostly associated with angiogenesis, gluconeogenesis, and triacylglyceride catabolism. Conversely, 293 transcripts were down-regulated, associated to cholesterol biosynthesis, PPARα signaling, fatty acid biosynthesis, and glycolysis. This gene signature was concordant with hepatic metabolite levels and hepatic oxidative damage. Moreover, the increased plasmatic levels of AST (aspartate aminotransferase), ALT (alanine aminotransferase) and AP (alkaline phosphatase), as well as liver histology suggest stress-induced liver steatosis. This study offers an integrative molecular and biochemical analysis of the hepatic response to handling-stress, and reveals unknown aspects of lipid metabolism in a non-model teleost.http://journals.plos.org/plosone/article?id=10.1371/journal.pone.017644
GPS Tracking to Monitor the Spatiotemporal Dynamics of Cattle Behavior and Their Relationship with Feces Distribution
The sustainability of agrosilvopastoral systems, e.g., dehesas, is threatened. It is necessary to deepen the knowledge of grazing and its environmental impact. Precision livestock farming (PLF) technologies pose an opportunity to monitor production practices and their effects, improving decision-making to avoid or reduce environmental damage. The objective of this study was to evaluate the potential of the data provided by commercial GPS collars, together with information about farm characteristics and weather conditions, to characterize the distribution of cattle dung in paddocks, paying special attention to the identification of hotspots with an excessive nutrient load. Seven animals were monitored with smart collars on a dehesa farm located in Cordoba, Spain. Dung deposition was recorded weekly in 90 sampling plots (78.5 m2) distributed throughout the paddock. Grazing behavior and animal distribution were analyzed in relation to several factors, such as terrain slope, insolation or distance to water. Animal presence in sampling plots, expressed as fix, trajectory segment or time counting, was regressed with dung distribution. Cattle showed a preference for flat terrain and areas close to water, with selection indices of 0.30 and 0.46, respectively. The accumulated animal presence during the experimental period explained between 51.9 and 55.4% of the variance of dung distribution, depending on the indicator used, but other factors, such as distance to water, canopy cover or ambient temperature, also had a significant effect on the spatiotemporal dynamics of dung deposition. Regression models, including GPS data, showed determination coefficients up to 82.8% and were able to detect hotspots of dung deposition. These results are the first step in developing a decision support tool aimed at managing the distribution of dung in pastures and its environmental effects
HC4 Análisis De Costo-Efectividad De Las Vacunas Neumocócicas 13-Valente Y 23-Valente Para Adultos De Alto Riesgo En Colombia
PIN59 Cost Effectiveness Analysis Of Vaccination With 13-Valent (Pcv13) And 23-Valent (Ppsv23) Pneumococcal Vaccines For Adults In A Private Colombian Institution
Comparative pan-genome analysis of Piscirickettsia salmonis reveals genomic divergences within genogroups
Indexación: Scopus.Piscirickettsia salmonis is the etiological agent of salmonid rickettsial septicemia, a disease that seriously affects the salmonid industry. Despite efforts to genomically characterize P. salmonis, functional information on the life cycle, pathogenesis mechanisms, diagnosis, treatment, and control of this fish pathogen remain lacking. To address this knowledge gap, the present study conducted an in silico pan-genome analysis of 19 P. salmonis strains from distinct geographic locations and genogroups. Results revealed an expected open pan-genome of 3,463 genes and a core-genome of 1,732 genes. Two marked genogroups were identified, as confirmed by phylogenetic and phylogenomic relationships to the LF-89 and EM-90 reference strains, as well as by assessments of genomic structures. Different structural configurations were found for the six identified copies of the ribosomal operon in the P. salmonis genome, indicating translocation throughout the genetic material. Chromosomal divergences in genomic localization and quantity of genetic cassettes were also found for the Dot/Icm type IVB secretion system. To determine divergences between core-genomes, additional pan-genome descriptions were compiled for the so-termed LF and EM genogroups. Open pan-genomes composed of 2,924 and 2,778 genes and core-genomes composed of 2,170 and 2,228 genes were respectively found for the LF and EM genogroups. The core-genomes were functionally annotated using the Gene Ontology, KEGG, and Virulence Factor databases, revealing the presence of several shared groups of genes related to basic function of intracellular survival and bacterial pathogenesis. Additionally, the specific pan-genomes for the LF and EM genogroups were defined, resulting in the identification of 148 and 273 exclusive proteins, respectively. Notably, specific virulence factors linked to adherence, colonization, invasion factors, and endotoxins were established. The obtained data suggest that these genes could be directly associated with inter-genogroup differences in pathogenesis and host-pathogen interactions, information that could be useful in designing novel strategies for diagnosing and controlling P. salmonis infection. © 2017 Nourdin-Galindo, Sánchez, Molina, Espinoza-Rojas, Oliver, Ruiz, Vargas-Chacoff, Cárcamo, Figueroa, Mancilla, Maracaja-Coutinho and Yañez.https://www.frontiersin.org/articles/10.3389/fcimb.2017.00459/ful
A Low-Cost IoT-Based System to Monitor the Location of a Whole Herd
Animal location technologies have evolved considerably in the last 60 years. Nowadays, animal tracking solutions based on global positioning systems (GPS) are commercially available. However, existing devices have several constraints, mostly related to wireless data transmission and financial cost, which make impractical the monitorization of all the animals in a herd. The main objective of this work is to develop a low-cost solution to enable the monitorization of a whole herd. An IoT-based system, which requires some animals of the herd being fitted with GPS collars connected to a Sigfox network and the rest with low-cost Bluetooth tags, has been developed. Its performance has been tested in two commercial farms, raising sheep and beef cattle, through the monitorization of 50 females in each case. Several collar/tag ratios, which define the cost per animal of the solution, have been simulated. Results demonstrate that a low collar/tag ratio enable the monitorization of a whole sheep herd. A larger ratio is needed for beef cows because of their grazing behavior. Nevertheless, the optimal ratio depends on the purpose of location data. Large variability has been observed for the number of hourly and daily messages from collars and tags. The system effectiveness for the monitorization of all the animals in a herd has been certainly proved
Assessment of CD-105 as an Angiogenic Modulator in Odontogenic Myxomas and Dental Follicles
Aim. Odontogenic myxoma is a benign intraosseous neoplasm of the jaws, with a locally aggressive behavior and a high
recurrence rate. CD-105 is a homodimeric cell membrane glycoprotein and is a component of the TGF-β1 growth factor
receptor complex that modulates angiogenesis by regulating the proliferation, differentiation and cellular migration.
The aim of this study is to quantify the microvascular density of the odontogenic myxoma based on the expression of
CD-105. Materials and Methods. The analysis included 18 odontogenic myxoma and 18 dental follicles as controls. A
standard immunohistochemical procedure was performed with the CD-105 antibody. Five representative fields (40×)
of the odontogenic myxoma and the dental follicles were selected to determine the microvascular density, which was
then followed by a descriptive and comparative statistical analysis. Results. Dental follicles presented a significantly higher
microvascular density compared with odontogenic myxoma (P = .001). The odontogenic myxoma smaller than 3 cm
showed a greater microvascular density than those larger than 3 cm in size (P > .05), and the microvascular density was
lower in large odontogenic myxomas as compared with the dental follicles (P = .003). Conclusion. A weaker expression
of CD-105 in odontogenic myxoma might indicate a lower angiogenic activity, suggesting that vascular proliferation has
a limited role in the growth mechanisms and in the aggressive behavior of this neoplasm.peer-reviewe
Globally-Linked Vortex Clusters in Trapped Wave Fields
We put forward the existence of a rich variety of fully stationary vortex
structures, termed H-clusters, made of an increasing number of vortices nested
in paraxial wave fields confined by trapping potentials. However, we show that
the constituent vortices are globally linked, rather than products of
independent vortices. Also, they always feature a monopolar global wave front
and exist in nonlinear systems, such as Bose-Einstein condensates. Clusters
with multipolar global wave fronts are non-stationary or at best flipping.Comment: 4 pages, 5 PostScript figure
The biology and behavior of the longhorned beetle, Dectes texanus on sunflower and soybean
The biology and behavior of the longhorned beetle Dectes texanus LeConte (Coleoptera: Cerambycidae) was studied on two host plants that suffer economic losses from this pest; sunflower, Helianthus annuus, and soybean, Glycines max. Reciprocal crosses of D. texanus collected from the two plants all produced viable progeny, indicating that conspecific insects attack both crops. Pupae from soybean stalks weighed about 40% less than those from sunflower, and adults fed on soybean lived a mean of 23 days, compared to a mean of 53 days (males) and 76 days (females) for those fed sunflower. A female's larval host plant had no effect on her tendency to ovipuncture plants of either type in a greenhouse trial. A field-tested population collected exclusively from sunflower contained three types of females in similar proportions: those that laid eggs only on sunflower, those that laid only on soybean, and those that laid equally on both host plants. Females in field trials fed more on the plant they had fed on in the laboratory, but soybean-fed females fed more on soybean than did sunflower-fed females. Females fed soybean also made more ovipunctures on soybean plants in field trials than sunflower-fed females, but their responses to sunflower plants were similar. Females displayed higher total ovipositional activity when they encountered sunflower first in the field, and lower total activity when they encountered soybean first. Feeding scores were significantly correlated with ovipunctures and eggs on both plant types. We conclude that sunflower is the preferred host plant, although females will accept soybean when it is the only available food. The results suggest that D. texanus is still in the initial stages of a host range expansion with female host selection behavior demonstrating both genetic influences and phenotypic flexibility. Sunflower represents a nutritionally superior, ancestral host plant and relatively high fitness costs are still associated with utilization of the novel host plant, soybean, costs that may be offset by benefits such as reduced intraspecific competition. These potential benefits and their consequent implications for D. texanus host range evolution are hypothesized and discussed
Has the Universe always expanded ?
We consider a cosmological setting for which the currently expanding era is
preceded by a contracting phase, that is, we assume the Universe experienced at
least one bounce. We show that scalar hydrodynamic perturbations lead to a
singular behavior of the Bardeen potential and/or its derivatives (i.e. the
curvature) for whatever Universe model for which the last bounce epoch can be
smoothly and causally joined to the radiation dominated era. Such a Universe
would be filled with non-linear perturbations long before nucleosynthesis, and
would thus be incompatible with observations. We therefore conclude that no
observable bounce could possibly have taken place in the early universe if
Einstein gravity together with hydrodynamical fluids is to describe its
evolution, and hence, under these conditions, that the Universe has always
expanded.Comment: 11 pages, LaTeX-ReVTeX, no figures, submitted to PR
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