Effect of prolonging radiation delivery time on retention of gammaH2AX

<p>Abstract</p> <p>Background and purpose</p> <p>Compared to conventional external beam radiotherapy, IMRT requires significantly more time to deliver the dose. Prolonging dose delivery potentially increases DNA repair which would reduce the biological effect. We questioned whether retention of γH2AX, a measure of lack of repair of DNA damage, would decrease when dose delivery was protracted.</p> <p>Materials and methods</p> <p>Exponentially growing SiHa cervical carinoma cells were irradiated with 6 MV photons in a water tank using a VarianEX linear accelerator. Cells held at 37°C received 2 Gy in 0.5 min and 4 Gy in 1 min. To evaluate effect of dose delivery prolongation, 2 and 4 Gy were delivered in 30 and 60 min. After 24 h recovery, cells were analyzed for clonogenic survival and for residual γH2AX as measured using flow cytometry.</p> <p>Results</p> <p>Increasing the dose delivery time from 0.5 or 1 min to 30 or 60 min produced a signficant increase in cell survival from 0.45 to 0.48 after 2 Gy, and from 0.17 to 0.20 after 4 Gy. Expression of residual γH2AX decreased from 1.27 to 1.22 relative to background after 2 Gy and 1.46 to 1.39 relative to background after 4 Gy, but differences were not statistically significant. The relative differences in the slopes of residual γH2AX versus dose for acute versus prolonged irradiation bordered on significant (p = 0.055), and the magnitude of the change was consistent with the observed increase in surviving fraction.</p> <p>Conclusion</p> <p>These results support the concept that DNA repair underlies the increase in survival observed when dose delivery is prolonged. They also help to establish the limits of sensitivity of residual γH2AX, as measured using flow cytometry, for detecting differences in response to irradiation.</p

Investigation of the Chirikov resonance overlap criteria for equatorial magnetosonic waves

Observations of equatorial magnetosonic waves made during the Cluster I nnerMagnetospheric Campaign clearly show discrete spectra consisting of emissions around harmonics of theproton gyrofrequency. Equatorial magnetosonic waves are important because of their ability to efficientlyscatter electrons in energy and pitch angle. This wave-particle interaction is numerically modeled throughthe use of diffusion coefficients, calculated based on a continuous spectrum such as that observed byspectrum analyzers. Using the Chirikov overlap resonance criterion, the calculation of the diffusioncoefficient will be assessed to determine whether they should be calculated based on the discrete spectralfeatures as opposed to a continuous spectrum. For the period studied, it is determined that the discretenature of the waves does fulfill the Chirikov overlap criterion and so the use of quasi-linear theory with theassumption of a continuous frequency spectrum is valid for the calculation of diffusion coefficients

Cosmic Rays from PeV to ZeV, Stellar Evolution, Supernova Physics and Gamma Ray Bursts

The recent success of a proposal from some time ago to explain the spectrum of cosmic rays allows some strong conclusions to be made on the physics of supernovae: In the context of this specific proposal to explain the origin of cosmic rays, the mechanism for exploding supernovae of high mass has to be the one proposed by Bisnovatyi-Kogan more than 30 years ago, which was then based on a broader suggestion by Kardashev: A combination of the effects of rotation and magnetic fields explodes the star. Interestingly, this step then leads inevitably to some further suggestions, useful perhaps for the study of gamma ray bursts and the search of a bright standard candle in cosmology

The supernova remnant CTB 37B and its associated magnetar CXOU J171405.7-381031: evidence for a magnetar-driven remnant

We discuss in this Letter the association of the candidate magnetar CXOU J171405.7-381031 with the supernova remnant CTB 37B. The recent detection of the period derivative of the object allowed an estimation of a young characteristic age of only $\sim 1000 yr$. This value is too small to be compatible even with the minimum radius of the remnant $\geq 10 pc$, the value corresponding to the {\it lower} limit of the estimated distance of $10.2 \pm 3.5 kpc$, unless the true distance happens to be even smaller than the lower limit. We argue that a consistent scenario for the remnant origin, in which the latter is powered by the energy injected by a young magnetar, is indeed more accurate to explain the young age, and points out to its non-standard (i.e. magnetar-driven) nature.Comment: 6 pp., 1 figure, to appear in RAA Letter

Influence of stress concentrator shape and testing temperature on impact fracture regularities of pipeline steel

The structure and impact toughness of the pipeline 17Mn1Si steel have been studied. The main attention was paid to the analysis of various conditions of stress concentration under dynamic loadings. The process of strain localization with increasing stress state stiffness at the tip of the concentrator with decreasing testing temperature was investigated. Impact loading diagrams for specimens with various stress concentrator shapes were registered and analyzed

A supersymmetric model for triggering Supernova Ia in isolated white dwarfs

We propose a model for supernovae Ia explosions based on a phase transition to a supersymmetric state which becomes the active trigger for the deflagration starting the explosion in an isolated sub-Chandrasekhar white dwarf star. With two free parameters we fit the rate and several properties of type Ia supernovae and address the gap in the supermassive black hole mass distribution. One parameter is a critical density fit to about $3 \cdot 10^7$ g/cc while the other has the units of a space time volume and is found to be of order $0.05\,$ Gyr $R_E^3$ where $R_E$ is the earth radius. The model involves a phase transition to an exact supersymmetry in a small core of a dense star.Comment: 20 pages, 5 figures, expanded version to be published in Physical Review

Material fragmentation as dissipative process of micro rotation sequence formation: Hybrid model of excitable cellular automata

The authors have developed a multi-level model of energy propagation along interfaces between the various structural elements of a solid with taking into account mutual energy transformations of various kinds. They have also designed a computer simulation tool based on the excitable cellular automaton (ECA) method. An algorithm for calculating the local moments of forces has been developed for the case of material rotation and torsion. The relationship for the accumulated elastic energy is supplemented with a dissipation term. Numerical experiments have been carried out on high-energy impact on polycrystalline copper specimens with different grain sizes. The paper shows that during the nanostructuring of material surface layer, the dissipation of elastic energy gives rise to the rotation of structural elements. This makes it possible to prevent the occurrence of stress concentrators with peak values typical of coarse-grained specimens and reducing their mechanical properties

Influence of stress concentrator shape and testing temperature on impact fracture regularities of pipeline steel

The structure and impact toughness of the pipeline 17Mn1Si steel have been studied. The main attention was paid to the analysis of various conditions of stress concentration under dynamic loadings. The process of strain localization with increasing stress state stiffness at the tip of the concentrator with decreasing testing temperature was investigated. Impact loading diagrams for specimens with various stress concentrator shapes were registered and analyzed

Application of ESI FT-ICR MS to Study Kraft Lignin Modification by the Exoenzymes of the White Rot Basidiomycete Fungus TrametesHirsutaLE-BIN 072

Trameteshirsuta is a wood rotting fungus that possesses a vast array of lignin degrading enzymes, including7 laccases, 7 ligninolyticmanganese peroxidases, 9 lignin peroxidases and 2 versatile peroxidases. In this study,electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI FT-ICR MS)was used to examine kraft lignin modification by the enzymatic system of this fungus.The observed pattern of lignin modification suggested that before the 6th day of cultivation,the fungal enzymatic system tended to degrade more oxidized moleculesand, hence, less recalcitrant molecules, with the production of hard-to-modify reduced molecular species. At some point after the 6th day of cultivation,the fungal enzymatic system tended to degrade more oxidized moleculesand, hence, less recalcitrant molecules, with the production of hard-to-modify reduced molecular species. At some point after the 6th day of cultivation,the fungus started to degrade less oxidized, more recalcitrant, compounds, converting them into the more oxidized forms. The altered pattern of lignin modification enabled changes in the fungal enzymatic system. These changes were further attributed to the appearance of the particular ligninolyticmanganese peroxides enzyme(MnP7), which was added by the fungus to the mixture of enzymes that had already been secreted (VP2 and MnP5). Keywords: wood rotting fungi, kraft lignin, mass spectrometry, peroxidase