Potency of partially purified anthocyanin from leaf extract of Guiera senegalensis against carbon tetrachloride-induced lipoperoxidation in rats

Anthocyanin was extracted and partially purified from the ethanolic leaf extract of Guiera senegalensis (GS). The recovered anthocyanin extract was found to have a concentration of 8mg/cm3 and an absorption spectrum with peak at 268nm. In order to assess the preventive action of the anthocyanin against lipoperoxidation, serum malondialdehyde (MDA) was analyzed in rats administered with 1mg/kg of the anthocyanin before induction of lipoperoxidation in the rats using carbon tetrachloride (CC14). Serum MDA was also analyzed in rats administered with 1 and 2 mg/kg of anthocyanin for up to seven (7) days after induction of lipoperoxidation in the rats in order to determine the curative effect of the extract. The results suggest that anthocyanin extracted from GS leaves are more effective in the cure than prevention of lipoperoxidation, and the most effective daily dose of treatment is 1mg/kg for 7 days. Key words: Guiera senegalensis, Anthocyanin, Potency, Lipoperoxidatio

Phytochemical screening and antimicrobial efficacy of Alternanthera nodiflora extracts

The phytochemical constituents and antimicrobial activities of Alternanthera nodiflora extracts were analyzed. Plant sample was extracted using  methanol and water. Qualitative phytochemical screening revealed the presence of alkaloids, carotenoid, flavonoids, terpenoids, cardiac  glycosides, phenols and saponins while tannins were absent in both  extracts. The antimicrobial potential of the extracts was tested against Staphylococcus aureus, Escherichia coli and Salmonella typhi, Candidaalbicans and Aspergillus niger. The Susceptibility patterns of the test organisms to varying concentrations (100mg/ml, 75mg/ml, 50mg/ml and 25mg/ml) of both extracts were determined by Kirby Bauer method. From this study, antimicrobial activity of the plant extracts was highest at 100mg/ml with Methanolic extract having more antimicrobial activity than aqueous extract. The extracts showed high activity against Candida albicans but no activity was observed against Aspergilus niger while the highest antibacterial activity of the extract was observed against Staphylococcus aureus.The higher antimicrobial activity in methanolic extract than aqueous extracts could be attributed to the degree of polarity of the extraction solvent.Key words: Alternanthera nodiflora, Phytochemicals, Antimicrobial activity, Extracts

RECURRENT HYDATIDIFORM MOLE COMPLICATED BY TOXIC GOITRE.

Hyperthyroidism complicates few pregnancies, in many cases due to Graves' disease, Gestational trophoblastic disease is a rare cause of hyperthyroidism in which high levels of hCG causes activation of the thyrotrophin receptor to stimulate supraphysiological secretion of thyroid hormone with or without thyroid gland enlargement Molar pregnancies are usually not recurrent, however, women with a previous hydatidiform mole (HM) are at higher risk of having a second mole than women from the general population. After a prior molar pregnancy, the risk of having a second one is 540 times that of the general population, however familial molar pregnancies are exceedingly rare. Here we present a case of recurrent HM complicated by a toxic goiter in a patient with family history of molar pregnancy&nbsp

Carriage of linezolid-resistant enterococci (LRE) among humans and animals in Nigeria: coexistence of the cfr, optrA, and poxtA genes in Enterococcus faecium of animal origin

Objectives: In contrast to increasing reports of the emergence of linezolid-resistant enterococci (LRE) emanating from many countries in Europe, Asia, and North America, data on its status and dissemination from the African continent remain scarce, with the information available limited to countries in North Africa. This study investigated the carriage of LRE and the genetic mechanism of resistance among Enterococcus faecium and Enterococcus faecalis strains recovered from humans and animals in Makurdi, Nigeria. Methods: We conducted a cross-sectional study between June 2020 and July 2021 during which 630 nonduplicate human and animal faecal samples were collected and processed for the recovery of LRE. The genetic mechanisms for resistance were investigated using polymerase chain reaction (PCR) and Sanger sequencing. Results: Linezolid-resistant enterococci were recovered from 5.87% (37/630; 95% CI: 4.17–8.00) of the samples, with the prevalence in animals and humans being 6.22% [(28/450); 95% CI: 4.17–8.87] and 5.00% [(9/180); 95% CI: 2.31–9.28], respectively. All isolates remained susceptible to vancomycin. No known point mutation mediating linezolid resistance was detected in the 23S rRNA and ribosomal protein genes; however, acquisition of one or more potentially transferable genes (cfr, optrA, and poxtA) was observed in 26 of the 37 LRE isolates. Co-existence of all three transferable genes in a single isolate was found in four E. faecium strains of animal origin. Conclusion: This study provides baseline evidence for the emergence and active circulation of LRE driven majorly by the acquisition of the optrA gene in Nigeria. To the best of our knowledge, our study is the first to report a co-carriage of all three transferable linezolid resistance determinants in E. faecium. Active LRE surveillance is urgently required to understand the extent of LRE spread across sub-Saharan Africa and to develop tailored mitigation strategies

Identification of Adult Fasciola spp. Using Matrix-Assisted Laser/Desorption Ionization Time-of-Flight (MALDI-TOF) Mass Spectrometry

Fascioliasis is a neglected trematode infection caused by Fasciola gigantica and Fasciola hepatica. Routine diagnosis of fascioliasis relies on macroscopic identification of adult worms in liver tissue of slaughtered animals, and microscopic detection of eggs in fecal samples of animals and humans. However, the diagnostic accuracy of morphological techniques and stool microscopy is low. Molecular diagnostics (e.g., polymerase chain reaction (PCR)) are more reliable, but these techniques are not routinely available in clinical microbiology laboratories. Matrix-assisted laser/desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) is a widely-used technique for identification of bacteria and fungi; yet, standardized protocols and databases for parasite detection need to be developed. The purpose of this study was to develop and validate an in-house database for Fasciola species-specific identification. To achieve this goal, the posterior parts of seven adult F. gigantica and one adult F. hepatica were processed and subjected to MALDI-TOF MS to create main spectra profiles (MSPs). Repeatability and reproducibility tests were performed to develop the database. A principal component analysis revealed significant differences between the spectra of F. gigantica and F. hepatica. Subsequently, 78 Fasciola samples were analyzed by MALDI-TOF MS using the previously developed database, out of which 98.7% (n = 74) and 100% (n = 3) were correctly identified as F. gigantica and F. hepatica, respectively. Log score values ranged between 1.73 and 2.23, thus indicating a reliable identification. We conclude that MALDI-TOF MS can provide species-specific identification of medically relevant liver flukes

Regional water cycle sensitivity to afforestation: synthetic numerical experiments for tropical Africa

Afforestation as a climate change mitigation option has been the subject of intense debate and study over the last few decades, particularly in the tropics where agricultural activity is expanding. However, the impact of such landcover changes on the surface energy budget, temperature, and precipitation remains unclear as feedbacks between various components are difficult to resolve and interpret. Contributing to this scientific debate, regional climate models of varying complexity can be used to test how regional climate reacts to afforestation. In this study, the focus is on the gauged Nzoia basin (12,700 km2) located in a heavily farmed region of tropical Africa. A reanalysis product is dynamically downscaled with a coupled atmospheric-hydrological model (WRF-Hydro) to finely resolve the land-atmosphere system in the Nzoia region. To overcome the problem of Nzoia river flooding over its banks we enhance WRF-Hydro with an overbank flow routing option, which improves the representation of daily discharge based on the Nash-Sutcliffe efficiency and Kling-Gupta efficiency (from −2.69 to 0.30, and −0.36 to 0.63, respectively). Changing grassland and cropland areas to savannas, woody savannas, and evergreen broadleaf forest in three synthetic numerical experiments allows the assessment of potential regional climate impacts of three afforestation strategies. In all three cases, the afforestation-induced decrease in soil evaporation is larger than the afforestation-induced increase in plant transpiration, thus increasing sensible heat flux and triggering a localized negative feedback process leading to more precipitation and more runoff. This effect is more pronounced with the woody savannas experiment, with 7% less evapotranspiration, but 13% more precipitation, 8% more surface runoff, and 12% more underground runoff predicted in the Nzoia basin. This study demonstrates a potentially large impact of afforestation on regional water resources, which should be investigated in more detail for policy making purposes

Regional water cycle sensitivity to afforestation: synthetic numerical experiments for tropical Africa

Afforestation as a climate change mitigation option has been the subject of intense debate and study over the last few decades, particularly in the tropics where agricultural activity is expanding. However, the impact of such landcover changes on the surface energy budget, temperature, and precipitation remains unclear as feedbacks between various components are difficult to resolve and interpret. Contributing to this scientific debate, regional climate models of varying complexity can be used to test how regional climate reacts to afforestation. In this study, the focus is on the gauged Nzoia basin (12,700 km2) located in a heavily farmed region of tropical Africa. A reanalysis product is dynamically downscaled with a coupled atmospheric-hydrological model (WRF-Hydro) to finely resolve the land-atmosphere system in the Nzoia region. To overcome the problem of Nzoia river flooding over its banks we enhance WRF-Hydro with an overbank flow routing option, which improves the representation of daily discharge based on the Nash-Sutcliffe efficiency and Kling-Gupta efficiency (from −2.69 to 0.30, and −0.36 to 0.63, respectively). Changing grassland and cropland areas to savannas, woody savannas, and evergreen broadleaf forest in three synthetic numerical experiments allows the assessment of potential regional climate impacts of three afforestation strategies. In all three cases, the afforestation-induced decrease in soil evaporation is larger than the afforestation-induced increase in plant transpiration, thus increasing sensible heat flux and triggering a localized negative feedback process leading to more precipitation and more runoff. This effect is more pronounced with the woody savannas experiment, with 7% less evapotranspiration, but 13% more precipitation, 8% more surface runoff, and 12% more underground runoff predicted in the Nzoia basin. This study demonstrates a potentially large impact of afforestation on regional water resources, which should be investigated in more detail for policy making purposes.publishedVersio

Loss of balancing selection in the βS globin locus

<p>Abstract</p> <p>Background</p> <p>Probably the best example of the rise and maintenance of balancing selection as an evolutionary trend is the role of S-haemoglobin (HbS - rs334) in protecting from malaria. Yet, the dynamics of such a process remains poorly understood, particularly in relation to different malaria transmission rates and the genetic background of the affected populations.</p> <p>Methods</p> <p>We investigated the association of haemoglobin HbS in protection from clinical episodes of malaria in two populations/villages where malaria is endemic, but mostly presenting in mild clinical forms. Five-hundred and forty-six individuals comprising 65 and 82 families from the Hausa and Massalit villages respectively were genotyped for HbS. Allele and genotype frequencies as well as departure from Hardy-Weinberg Equilibrium were estimated from four-hundred and seventy independent genotypes across different age groups. Age-group frequencies were used to calculate the coefficient-of-fitness and to simulate the expected frequencies in future generations.</p> <p>Results</p> <p>Genotype frequencies were within Hardy-Weinberg expectations in Hausa and Massalit in the total sample set but not within the different age groups. There was a trend for a decrease of the HbS allele frequency in Hausa and an increase of frequency in Massalit. Although the HbS allele was able to confer significant protection from the clinical episodes of malaria in the two populations, as suggested by the odds ratios, the overall relative fitness of the HbS allele seems to have declined in Hausa.</p> <p>Conclusions</p> <p>Such loss of balancing selection could be due to a combined effect of preponderance of non-clinical malaria in Hausa, and the deleterious effect of the homozygous HbS under circumstances of endogamy.</p

Kepercayaan pelajar terhadap konsep ubudiyyah dalam pengurusan halal / Mahfuzah Mohammed Zabidi … [et al.]

Pengurusan halal adalah satu bidang kritikal yang perlu diberikan perhatian serius demi merealisasikan Malaysia sebagai salah sebuah hab halal dunia. Bidang ini bertujuan memastikan kesucian dan sifat “patuh syariah” bagi sesuatu produk atau perkhidmatan terus terpelihara. Graduan bidang pengurusan halal diharapkan berperanan secara langsung dalam memenuhi keperluan badan pensijilan halal. Pelajar bidang ini perlu dipersiapkan dengan kefahaman mengenai konsep halal secara bersepadu dengan nilai ubudiyyah yang mendasari akidah, syariah dan tasawuf dalam Islam. Hal ini bagi memastikan mereka dapat memberi kefahaman yang jelas kepada masyarakat, khususnya kepada penggiat industri bahawa halal bukan sekadar pada label atau logo semata-mata. Kajian ini bertujuan meneroka kepercayaan pelajar Diploma Pengurusan Halal, Akademi Pengajian Islam Kontemporari (ACIS) Universiti Teknologi MARA mengenai konsep ubudiyyah dan kesepaduannya dalam pengurusan halal. Kajian ini menggunakan pendekatan kualitatif menerusi temubual kumpulan fokus berpandukan protokol separa berstruktur. Data temubual dianalisis secara separa transkrip. Temubual mendapati bahawa pelajar lebih banyak menghubungkan konsep ubudiyyah dalam pengurusan halal dengan aspek syariat berbanding aspek akidah dan tasawuf. Oleh itu, kesepaduan ilmu antara kursus-kursus akademik yang ditawarkan oleh fakulti, sama ada dalam pengajian ilmu Islam mahupun pengajian ilmu moden yang berteraskan nilai ubudiyyah dalam bidang pengurusan halal dilihat perlu diperkemaskan bagi mendepani isu halal secara lebih holistik. Natijahnya, kesepaduan ilmu ini akhirnya berupaya menzahirkan Islam sebagai agama rahmat untuk sekalian alam

Identification of High-Yielding Iron-Biofortified Open-Pollinated Varieties of Pearl Millet in West Africa

Pearl millet is a predominant food and fodder crop in West Africa. This study was carried out to test the newly developed open-pollinated varieties (OPVs) for field performance and stability for grain yield, grain iron (Fe), and grain zinc (Zn) contents across 10 locations in West Africa (i.e., Niger, Nigeria, Mali, Burkina Faso, Senegal, and Ghana). The test material consisted of 30 OPVs, of which 8 are Fe/Zn biofortified. The experiment was conducted in a randomized complete block design in three replications. ANOVA revealed highly significant variability for grain yield and micronutrient traits. The presence of genotype x environment (G x E) indicated that the expressions of traits are significantly influenced by both genetic and G x E factors, for grain Fe and Zn contents. Days to 50% flowering and plant height showed less G x E, suggesting these traits are largely under genetic control. The genotypes CHAKTI (46 days), ICTP 8203 (46 days), ICMV 177002 (50 days), ICMV 177003 (48 days), and Moro (53 days) had exhibited early flowering across locations leading to early physiological maturity. CHAKTI (1.42 t/ha yield; 62.24 mg/kg of grain Fe, 47.29 mg/kg of grain Zn) and ICMP 177002 (1.19 t/ha yield, 62.62 mg/kg of grain Fe, 46.62 mg/kg of grain Zn) have performed well for grain yield and also for micronutrients, across locations, compared with the check. Additive Main Effect and Multiplicative Interaction (AMMI) ANOVA revealed the highly significant genotypic differences, the mean sum of squares of environment, and its interaction with the genotypes. Based on the AMMI stability value (ASV), the most stable genotype is SOSAT-C88 (ASV = 0.04) for grain yield and resistance to downy mildew; mean grain yield and stability rankings (YSI) revealed that the genotypes CHAKTI, SOSAT-C88, and ICMV IS 99001 were high yielding and expressed stability across regions. The strong correlation (r = 0.98**) of grain Fe and Zn contents that merits Fe-based selection is highly rewarding. CHAKTI outperformed over other genotypes for grain yield (71% higher), especially with early maturing varieties in West Africa, such as GB 8735, LCIC 9702, and Jirani, and for grain Fe (16.11% higher) and Zn (7% higher) contents across locations, and made a candidate of high-iron variety to be promoted for combating the micronutrient malnutrition in West and Central Africa (WCA)