Carriage of linezolid-resistant enterococci (LRE) among humans and animals in Nigeria: coexistence of the cfr, optrA, and poxtA genes in Enterococcus faecium of animal origin
Objectives: In contrast to increasing reports of the emergence of linezolid-resistant enterococci (LRE) emanating from many countries in Europe, Asia, and North America, data on its status and dissemination
from the African continent remain scarce, with the information available limited to countries in North
Africa. This study investigated the carriage of LRE and the genetic mechanism of resistance among Enterococcus faecium and Enterococcus faecalis strains recovered from humans and animals in Makurdi, Nigeria.
Methods: We conducted a cross-sectional study between June 2020 and July 2021 during which 630 nonduplicate human and animal faecal samples were collected and processed for the recovery of LRE. The
genetic mechanisms for resistance were investigated using polymerase chain reaction (PCR) and Sanger
sequencing.
Results: Linezolid-resistant enterococci were recovered from 5.87% (37/630; 95% CI: 4.17–8.00) of the
samples, with the prevalence in animals and humans being 6.22% [(28/450); 95% CI: 4.17–8.87] and 5.00%
[(9/180); 95% CI: 2.31–9.28], respectively. All isolates remained susceptible to vancomycin. No known
point mutation mediating linezolid resistance was detected in the 23S rRNA and ribosomal protein genes;
however, acquisition of one or more potentially transferable genes (cfr, optrA, and poxtA) was observed in
26 of the 37 LRE isolates. Co-existence of all three transferable genes in a single isolate was found in four
E. faecium strains of animal origin.
Conclusion: This study provides baseline evidence for the emergence and active circulation of LRE driven
majorly by the acquisition of the optrA gene in Nigeria. To the best of our knowledge, our study is the
first to report a co-carriage of all three transferable linezolid resistance determinants in E. faecium. Active
LRE surveillance is urgently required to understand the extent of LRE spread across sub-Saharan Africa
and to develop tailored mitigation strategies