Vinyl sulfone-based ferrocenylation reagents: applications in conjugation and bioconjugation

The easy vinyl sulfone derivatization of ferrocene allows the preparation of some effective, versatile and valuable ferrocenylation reagents. The applicability of such compounds in conjugation and bioconjugation of amine and/or thiol containing molecules and biomolecules through Michael-type addition under mild conditions that preserve the biological function of the latter is described. The feasibility of the methodology is demonstrated by the preparation of a variety of conjugates and bioconjugates (ferrocenyl terminated dendrimers and ferrocene–sugar, ferrocene–cyclodextrin, ferrocene–peptide and ferrocene–protein conjugates).Financial support was provided by Ministerio de Ciencia e Innovación (CTQ2011-29299-CO2-01)

Non-wage instruments for motivating employees in public organizations on the example of the Municipal Office in Skarżysko-Kamienna

W niniejszej pracy zostały przedstawione czynniki motywacji, które zaliczają się do ważniejszych funkcji zarządzania zasobami ludzkimi. Motywowanie stanowi nieodłączny element zarządzania, oraz jest częścią kluczowych czynników, dzięki którym organizacje mogą poprawnie funkcjonować. W pracy opisano motywacje pozapłacową jak również płacową. Na podstawie przeprowadzonego badania wykonanego w formie ankiety, w części empirycznej skoncentrowano się na czynnikach wpływających na motywację do pracy, oraz opisano kontekst postrzegania pracy przez pracowników jak i przełożonych.The main thesis presents motivation factors that are among the most important functions of human resources management. Motivating is an integral part of management, and is part of the key factors that allow organizations to function properly. The work describes non-wage motivations as well as wages. Based on the survey carried out in the form of a survey, the empirical part focuses on factors influencing the motivation to work, and describes the context of perception of work by employees and superiors

The presence of a ferrocenyl unit on an estrogenic molecule is not always sufficient to generate in vitro cytotoxicity

We recently reported the dual (antihormonal and cytotoxic) functionality of ferrocifens, which are organometallic complexes derived from hydroxytamoxifen, the std. mol. in the treatment of hormone-dependent breast cancers. To test the hypothesis that the presence of a ferrocenyl substituent on mols. with an affinity for the estrogen receptor is sufficient to give them cytotoxic properties in vitro, we prepd. complexes derived from estradiol with a ferrocenyl substituent at positions 7α and 17α. The complexes thus obtained retain a satisfactory level of affinity for the estrogen receptor (RBA values higher than 12 %). At low concns. (0.1-1 μM) the complexes show an estrogenic effect in vitro equiv. to that of estradiol on hormone-dependent (MCF-7) breast cancer cells, and no cytotoxic effect on hormone-independent (MDA-MB-231) breast cancer cells. At high concns. (up to 50 μM) the 17α-ethynylferrocenyl estradiol and 7α-ferrocenylmethylthio estradiol become cytotoxic (IC50 = 13.2 μM and 18.8 μM, resp.) while the 17α-ferrocenylestradiol remains non toxic. The low toxicity of these compds. support our hypothesis that electronic communication between the ferrocenyl and phenol moieties in the hydroxyferrocifens series is a key parameter in the generation of cytotoxic effects at submicromolar concns