La població de la zona urbana a Badalona

Sota l'aixopluc del Programa Urban que la Comunitat Europea ha donat a la zona de la Serra d'en Mena de la ciutat de Badalona, la Fundació Bofill ha realitzat un seguit d'estudis sobre l'estructura social del territori i especialment sobre els grups socials més desafavorits d'aquest territori. El que es presenta en aquesta comunicació són els diferents resultats en el coneixement d'aquests grups a què s'ha arribat a partir d'anàlisis de diferent tipus, qualitatives i quantitatives, i de l'especificitat del seu assentament en el territori

Development of a highly efficient production process for recombinant protein expression in Escherichia coli NEB10β

Altres ajuts: COST Action CM 1303 - Systems BiocatalysisRecombinant protein expression in E. coli is well described, with multiple strains and process strategies available. However, strains used for cloning and molecular biology purposes are not generally considered for protein expression. Using these strains could result in a simplification of the production pathway of a newly cloned protein of interest. In this work, the E. coli strain NEB10β has been characterized for the expression of the complex fusion protein phosphite dehydrogenase-cyclohexanone monooxygenase (PTDH-CHMO), and a production process has been developed based on the PBAD expression system. A fed-batch approach using a defined medium supplemented with amino acids, with glycerol as a carbon source, allows for an efficient recombinant protein expression process, incrementing 9.2-fold the production obtained in a complex medium batch and reaching around 2 g/L of product after 6 h of induction. The process was successfully reproduced in a NEB10β strain for the production of the alcohol dehydrogenase (ADH) enzyme

Implementación de una herramienta didáctica para la manipulación de autómatas finitos y expresiones regulares

En este trabajo presentamos una herramienta que pretende facilitar la labor docente de la asignatura Lenguajes, gramáticas y autómatas, y en particular el uso y manejo de autómatas finitos y expresiones regulares. Esta aplicación permite completar el temario de la asignatura ofreciendo al estudiante la posibilidad de realizar prácticas en el laboratorio, encaminadas tanto a la manipulación y comprensión de los objetos presentados en el aula, como a la incorporación de nuevos algoritmos

Nonexistence of almost Moore digraphs of diameter four

Regular digraphs of degree d > 1, diameter k > 1 and order N(d; k) = d+ +dk will be called almost Moore (d; k)-digraphs. So far, the problem of their existence has only been solved when d = 2; 3 or k = 2; 3. In this paper we prove that almost Moore digraphs of diameter 4 do not exist for any degree dPostprint (published version

Effects on nasal nitric oxide production of 2 mechanisms of vasoconstriction

Background: Vasoconstrictor drugs reduce nitric oxide (NO) production in vitro by inhibiting the enzyme involved in the regulation of inducible and constitutive NO synthases (iNOS and cNOS). Intranasal vasoconstrictors also decrease nasal NO concentration in vivo. It is as yet unclear if this last finding is due to the effects of the drug on the enzyme or on the vessels. Physical exercise also induces nasal vasoconstriction and reduces nasal resistance. Objectives: The aim of this study was to clarify the mechanisms involved in xylometazoline-induced reduction of nasal NO concentration. Methods: We compared 2 randomized groups of patients with moderate-severe persistent allergic rhinitis. The fi rst group (n=24) underwent a physiological nasal vasoconstrictor stimulus (exercise) whereas the second group (n=29) was treated with a nasal vasoconstrictor drug (topical xylometazoline). Nasal volume and NO were determined at baseline and 15 to 20 minutes after the end of each stimulus using acoustic rhinometry and chemiluminescence, respectively. Results: Baseline values of nasal volume and NO did not differ between the 2 groups. Nasal volume increased by 57% (P = .0001) after exercise and 71% (P = .0001) after xylometazoline. Nasal NO decreased (25%, P = .001) after xylometazoline, but not after exercise. Conclusion: Physical exercise and topical xylometazoline cause vasoconstriction and similar effects on nasal volume. In contrast nasal NO decreased with xylometazoline but not after exercise. These fi ndings suggest that vasoconstrictor drugs reduce nasal NO by mechanisms other than vasoconstriction