120 research outputs found

    La teoría de la acción social en Erving Goffman

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    El presente artículo ofrece una reflexión sobre una figura clave en sociología como es Goffman. Proponemos un viaje a través de su obra con el fin de profundizar en su modelo dramatúrgico. Acción ubicada, presentación del self, frames o interacción social, son elementos abordados a través de la comparativa con otros autores, entre los que se encuentran Parsons, Durkheim e incluso el propio Freud. La obra de Goffman se ha analizado desde múltiples perspectivas, dando lugar a diversas interpretaciones, pero sin lugar a dudas lo que emerge de ella es una continua pretensión de dibujar el difícil y complejo encuentro entre lo micro y lo macro, sin poder encasillarse en ninguna corriente teórica conocida.This article is a reflection on an outstanding figure in Sociology: Goffman. We suggest a view of its works in order to go deeply into its dramatist model. Located action, presentation of self, frame on social interaction are some of the elements we dead with though a comparative with other authors, such as Parsons, Durkheim and even Freud. Goffman's work has been analysed from numerous perspectives, offering several interpretations, but what remains still is, without any doubt, a constant aspiration of drawing the difficult and complex encounter between the micro and the macro, without being categorized in any of the well-knowm theoretical trends

    Evaluación de la calidad del suelo en diferentes agroecosistemas y determinación de indicadores para un manejo sostenible

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    Presentación Proyecto S.O.S. Suelo enmarcado dentro de los proyectos demostrativos AGROALNEXT, vinculados a la salud global, la evaluación y mejora de la salud de los suelos y a el manejo agroecológico de los principales cultivos aragoneses. Liderados por de El Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA

    Non-canonical Translation in Plant RNA Viruses

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    Viral protein synthesis is completely dependent upon the host cell's translational machinery. Canonical translation of host mRNAs depends on structural elements such as the 5′ cap structure and/or the 3′ poly(A) tail of the mRNAs. Although many viral mRNAs are devoid of one or both of these structures, they can still translate efficiently using non-canonical mechanisms. Here, we review the tools utilized by positive-sense single-stranded (+ss) RNA plant viruses to initiate non-canonical translation, focusing on cis-acting sequences present in viral mRNAs. We highlight how these elements may interact with host translation factors and speculate on their contribution for achieving translational control. We also describe other translation strategies used by plant viruses to optimize the usage of the coding capacity of their very compact genomes, including leaky scanning initiation, ribosomal frameshifting and stop-codon readthrough. Finally, future research perspectives on the unusual translational strategies of +ssRNA viruses are discussed, including parallelisms between viral and host mRNAs mechanisms of translation, particularly for host mRNAs which are translated under stress conditions.The research program in Aranda's lab is supported by grants AGL2015-65838 (MINECO, Spain) and ARIMNet2-EMERAMB(ERA-Net-618127, EU FP7). WM is funded by NIH grant number R01 GM067104.Peer reviewedPeer Reviewe

    Demostración del DSS sobre riego

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    WANUGRAPE 4.0 es un proyecto de I+D+i PDC2021-121210-C21 financiado por la Agencia Estatal de Investigación 10.13039/501100011033 por medio de la convocatoria Prueba de Concepto 2021 y cofinanciado por la Unión Europea con fondos Next Generation

    Cuadernos del Tábano 8

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    Revista de literatura y estudios sociales publicada en Alicante desde 2003 a 2010

    Liquid Biopsy in Neurological Diseases

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    Liquid biopsy; MicroRNA; Neurological diseasesBiòpsia líquida; MicroARN; Malalties neurològiquesBiopsia líquida; MicroARN; Enfermedades neurológicasThe most recent and non-invasive approach for studying early-stage biomarkers is liquid biopsy. This implies the extraction and analysis of non-solid biological tissues (serum, plasma, saliva, urine, and cerebrospinal fluid) without undergoing invasive procedures to determine disease prognosis. Liquid biopsy can be used for the screening of several components, such as extracellular vesicles, microRNAs, cell-free DNA, cell-free mitochondrial and nuclear DNA, circulating tumour cells, circulating tumour DNA, transfer RNA, and circular DNA or RNA derived from body fluids. Its application includes early disease diagnosis, the surveillance of disease activity, and treatment response monitoring, with growing evidence for validating this methodology in cancer, liver disease, and central nervous system (CNS) disorders. This review will provide an overview of mentioned liquid biopsy components, which could serve as valuable biomarkers for the evaluation of complex neurological conditions, including Alzheimer’s disease, Parkinson’s disease, amyotrophic lateral sclerosis, multiple sclerosis, epilepsy, stroke, traumatic brain injury, CNS tumours, and neuroinfectious diseases. Furthermore, this review highlights the future directions and potential limitations associated with liquid biopsy.S.M. was supported by the grant from Instituto Salud Carlos III (PI20/01697)

    Determination of the Secondary Structure of an RNA fragment in Solution: Selective 2`- Hydroxyl Acylation Analyzed by Primer Extension Assay (SHAPE)

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    This protocol describes the methodology for the determination of the secondary structure of an RNA fragment in solution using Selective 2´-Hydroxyl Acylation analyzed by Primer Extension, abbreviation SHAPE. It consists in the very fast chemical modification of flexible and therefore possibly single-stranded nucleotides in a sequence-independent manner using benzoyl cyanide (BzCN), forming 2´-O-adducts. The modifications in the RNA are then analyzed by primer extension. Reverse transcriptase is blocked by the 2´-O-adducts formed. The advantage of the method is, first, that not each RNA molecule studied but the primer used in the extension reaction is labelled and, second, that the resulting cDNA analyzed in sequencing gels is much more stable than the modified RN

    Cuadernos del Tábano 4

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    Revista de literatura y estudios sociales publicada en Alicante desde 2003 a 2010
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