The \u3csup\u3e18\u3c/sup\u3eO-Signal Transfer from Water Vapour to Leaf Water and Assimilates Varies Among Plant Species and Growth Forms

The 18O signature of atmospheric water vapour (δ18OV) is known to be transferred via leaf water to assimilates. It remains, however, unclear how the 18O‐signal transfer differs among plant species and growth forms. We performed a 9‐hr greenhouse fog experiment (relative humidity ≥ 98%) with 18O‐depleted water vapour (−106.7‰) on 140 plant species of eight different growth forms during daytime. We quantified the 18O‐signal transfer by calculating the mean residence time of O in leaf water (MRTLW) and sugars (MRTSugars) and related it to leaf traits and physiological drivers. MRTLW increased with leaf succulence and thickness, varying between 1.4 and 10.8 hr. MRTSugars was shorter in C3 and C4 plants than in crassulacean acid metabolism (CAM) plants and highly variable among species and growth forms; MRTSugars was shortest for grasses and aquatic plants, intermediate for broadleaf trees, shrubs, and herbs, and longest for conifers, epiphytes, and succulents. Sucrose was more sensitive to δ18OV variations than other assimilates. Our comprehensive study shows that plant species and growth forms vary strongly in their sensitivity to δ18OV variations, which is important for the interpretation of δ18O values in plant organic material and compounds and thus for the reconstruction of climatic conditions and plant functional responses

Updating the Dual C and O Isotope—Gas-exchange Model: A Concept to Understand Plant Responses to the Environment and Its Implications for Tree Rings

The combined study of carbon (C) and oxygen (O) isotopes in plant organic matter has emerged as a powerful tool for understanding plant functional responses to environmental change. The approach relies on established relationships between leaf gas exchange and isotopic fractionation to derive a series of model scenarios that can be used to infer changes in photosynthetic assimilation and stomatal conductance driven by changes in environmental parameters (CO2, water availability, air humidity, temperature, nutrients). We review the mechanistic basis for a conceptual model, in light of recently published research, and discuss where isotopic observations do not match our current understanding of plant physiological response to the environment. We demonstrate that (1) the model was applied successfully in many, but not all studies; (2) although originally conceived for leaf isotopes, the model has been applied extensively to tree-ring isotopes in the context of tree physiology and dendrochronology. Where isotopic observations deviate from physiologically plausible conclusions, this mismatch between gas exchange and isotope response provides valuable insights into underlying physiological processes. Overall, we found that isotope responses can be grouped into situations of increasing resource limitation versus higher resource availability. The dual-isotope model helps to interpret plant responses to a multitude of environmental factors

COMPUTER SIMULATIONS OF POSSIBLE FUTURES FOR TWO FLOCKS OF WHOOPING CRANES

We conducted computer simulations using the program VORTEX (version 7) to project population sizes, growth rates, genetic diversity, and probabilities of extinction over the next 100 years for 2 flocks of whooping cranes (Grus americana), the Aransas/Wood Buffalo population and the experimental Florida population. Standard runs based on best estimates of demographic. genetic, and environmental parameter values were used as a baseline to which several alternative scenarios were compared. Results generally supported the conclusion of the earlier Population Viability Assessment (Mirande et al. 1991) that the AransaslWood Buffalo population will continue to grow steadily with less than a 1 % probability of extinction. It was noted, however, that a combination of negative factors such as shrinking habitat and increased probabilities of catastrophes accompanied by increased mortality rates could put this population at risk. Results for the Florida population were less optimistic. The standard run produced a population growth rate (r) of only 0.0026 for the next 100 years, and this shifted down to -0.0001 over a 200-year time frame. Adult mortality in this flock would have to be about 20% lower than the predicted value (10%) in order to raise growth rates to above r = 0.02. Amount and duration of supplementation of the Florida flock had minimal impacts on the long-tenn growth rate of the flock. It is the enduring rates of mortality, breeding, and disease risk that will have major effects on this population. For example, if disease risks tum out to be greater than the best-estimate scenario, this population could face a relatively high risk of extinction (17%). The formula for success in Florida is lower adult mortality, lower age of first breeding, lower disease risk, and higher productivity than the best-guess estimates. Fortunately, there are some potential management interventions (e.g., predator control, vaccines and health monitoring, selective introductions to balance the sex ratio of the flock) that may be able to push the odds in favor of success

Molecular phylogeny of the subfamily Stevardiinae Gill, 1858 (Characiformes: Characidae): classification and the evolution of reproductive traits

Abstract Background The subfamily Stevardiinae is a diverse and widely distributed clade of freshwater fishes from South and Central America, commonly known as “tetras” (Characidae). The group was named “clade A” when first proposed as a monophyletic unit of Characidae and later designated as a subfamily. Stevardiinae includes 48 genera and around 310 valid species with many species presenting inseminating reproductive strategy. No global hypothesis of relationships is available for this group and currently many genera are listed as incertae sedis or are suspected to be non-monophyletic. Results We present a molecular phylogeny with the largest number of stevardiine species analyzed so far, including 355 samples representing 153 putative species distributed in 32 genera, to test the group’s monophyly and internal relationships. The phylogeny was inferred using DNA sequence data from seven gene fragments (mtDNA: 12S, 16S and COI; nuclear: RAG1, RAG2, MYH6 and PTR). The results support the Stevardiinae as a monophyletic group and a detailed hypothesis of the internal relationships for this subfamily. Conclusions A revised classification based on the molecular phylogeny is proposed that includes seven tribes and also defines monophyletic genera, including a resurrected genus Eretmobrycon, and new definitions for Diapoma, Hemibrycon, Bryconamericus sensu stricto, and Knodus sensu stricto, placing some small genera as junior synonyms. Inseminating species are distributed in several clades suggesting that reproductive strategy is evolutionarily labile in this group of fishes.http://deepblue.lib.umich.edu/bitstream/2027.42/134621/1/12862_2015_Article_403.pd