PPP1R8 (protein phosphatase 1, regulatory (inhibitor) subunit 8)

Review on PPP1R8 (protein phosphatase 1, regulatory (inhibitor) subunit 8), with data on DNA, on the protein encoded, and where the gene is implicated

The protein phosphatase 1 regulator NIPP1 is essential for mammalian spermatogenesis

NIPP1 is one of the major nuclear interactors of protein phosphatase PP1. The deletion of NIPP1 in mice is early embryonic lethal, which has precluded functional studies in adult tissues. Hence, we have generated an inducible NIPP1 knockout model using a tamoxifen-inducible Cre recombinase transgene. The inactivation of the NIPP1 encoding alleles (Ppp1r8) in adult mice occurred very efficiently in testis and resulted in a gradual loss of germ cells, culminating in a Sertoli-cell only phenotype. Before the overt development of this phenotype Ppp1r8 -/- testis showed a decreased proliferation and survival capacity of cells of the spermatogenic lineage. A reduced proliferation was also detected after the tamoxifen-induced removal of NIPP1 from cultured testis slices and isolated germ cells enriched for undifferentiated spermatogonia, hinting at a testis-intrinsic defect. Consistent with the observed phenotype, RNA sequencing identified changes in the transcript levels of cell-cycle and apoptosis regulating genes in NIPP1-depleted testis. We conclude that NIPP1 is essential for mammalian spermatogenesis because it is indispensable for the proliferation and survival of progenitor germ cells, including (un)differentiated spermatogonia.publishe

Use of master curve technology for assessing shallow flaws in a reactor pressure vessel material

In the NESC-IV project an experimental/analytical program was performed to develop validated analysis methods for transferring fracture toughness data to shallow flaws in reactor pressure vessels subject to biaxial loading in the lower-transition temperature region. Within this scope an extensive range of fracture tests was performed on material removed from a production-quality reactor pressure vessel. The Master Curve analysis of this data is reported and its application to the assessment of the project feature tests on large beam test pieces

Use of master curve technology for assessing shallow flaws in a reactor pressure vessel material

In the NESC-IV project, an experimental/analytical program was performed to develop validated analysis methods for transferring fracture toughness data to shallow flaws in reactor pressure vessels subject to biaxial loading in the lower-transition temperature region. Within this scope, an extensive range of fracture tests was performed on material removed from a production-quality reactor pressure vessel. The master curve analysis of these data is reported and its application to the assessment of the project feature tests on large beam test pieces is discussed

Sedimentary dynamics on isolated highs in Lake Baikal: evidence from detailed high-resolution geophysical data and sediment cores

High- and very-high-resolution seismic data, side-scan sonar mosaics and piston cores from three isolated highs in Lake Baikal (Vydrino Shoulder, Posolsky Bank, Continent Ridge) have allowed to document in unprecedented detail the depositional, re-depositional and tectonic processes and to characterise the overall sedimentary environment on such isolated highs. Our data show that Vydrino Shoulder actually represents a turbidity- or underflow-sourced slope fan, with a distinct channel-levee morphology, and affected by tectonics and stratigraphic unconformities. Sediment cores contain up to 90% of terrigenous material. Posolsky Bank is a major tilted fault block, clearly affected by tectonic activity, by stratigraphic unconformities and by mass wasting along its margins. Sedimentation on its crest is undisturbed, but average sedimentation rates are high due to the influence of the nearby Selenga River. Continent Ridge is also affected by active faulting, stratigraphic unconformities and by mass-wasting along its flanks. The area receives only 30% of terrigenous input. There are no signs of sediment reworking due to bottom-currents. Our study thus shows that in comparison with other environments in Lake Baikal, the isolated highs probably hold the best potential to contain a high-quality, continuous, undisturbed and undiluted sedimentary record suitable for paleoclimate research. (C) 2004 Elsevier B.V. All rights reserved.Continen

Kultivierungseffekte im Gesundheitsbereich

Der Kultivierungsansatz untersucht den langfristigen Einfluss des Fernsehens auf die Realitätsvorstellungen und Einstellungen der Rezipientinnen und Rezipienten. Im vorliegenden Beitrag werden zunächst die Grundannahmen des Ansatzes skizziert und dann ein Überblick über den Forschungsstand zu Kultivierungseffekten im Gesundheitsbereich gegeben. Dabei wird unterschieden nach Studien zum Ärztebild, zu Ernährung und Körperbild, zu Genuss- bzw. Suchtmitteln und zu Krankheiten