Os contaminantes na colmeia: controlo de resíduos

O mel é um produto alimentar que aufere da sociedade um reconhecimento como produto natural". Este e efectivamente um "rótulo” que lhe confere vantagens comerciais e que lodos as agentes do sector devem potenciar através de um trabalho rigoroso nas metodologias de produção, garantindo a qualidade do produto final

Direito das sociedades comerciais (capítulo I): sumários desenvolvidos das aulas de Direito para a Economia e Gestão

1. Noções fundamentais 1.1. A sociedade como ato e como entidade 1.2. Os elementos essenciais do conceito de sociedade 1.3. Os requisitos de comercialidade das sociedades. As sociedades civis, as sociedades civis sob forma comercial e as sociedades comerciais 2. O princípio da tipicidade das sociedades comerciais. Tipos de sociedades comerciais (em especial as sociedades por quotas e anónimas) e suas características fundamentais 3. O processo de constituição das sociedades 3.1. O princípio da contratualidade e suas excepções 3.2. O processo constitutivo de uma sociedade comercial 3.3. O regime jurídico aplicável em caso de incompletude do processo constitutivo 3.4. Regime jurídico relativo à invalidade do acto constitutivo 4. A sociedade como pessoa coletiva 4.1. Personalidade jurídica e autonomia patrimonial 4.2. Capacidade jurídica 5. Capital socia

Electrospun Polycaprolactone/Aloe Vera_Chitosan Nanofibrous Asymmetric Membranes Aimed for Wound Chelinga Applications

Today, none of the wound dressings available on the market is fully capable of reproducing all the features of native skin. Herein, an asymmetric electrospun membrane was produced to mimic both layers of skin. It comprises a top dense layer (manufactured with polycaprolactone) that was designed to provide mechanical support to the wound and a bottom porous layer (composed of chitosan and Aloe Vera) aimed to improve the bactericidal activity of the membrane and ultimately the healing process. The results obtained revealed that the produced asymmetric membranes displayed a porosity, wettability, as well as mechanical properties similar to those presented by the native skin. Fibroblast cells were able to adhere, spread, and proliferate on the surface of the membranes and the intrinsic structure of the two layers of the membrane is capable of avoiding the invasion of microorganisms while conferring bioactive properties. Such data reveals the potential of these asymmetric membranes, in the near future, to be applied as wound dressings

A genetic and evolutionary programming environment with spatially structured populations and built-in parallelism

The recent development of the Genetic and Evolutionary Computation field lead to a kaleidoscope of approaches to problem solving, which are based on a common background. These shared principles are used in order to develop a programming environment that enhances modularity, in terms of software design and implementation. The system's core encapsulates the main features of the Genetic and Evolutionary Algorithms, by identifying the entities at stake and implementing them as hierarchies of software modules. This architecture is enriched with the parallelization of the algorithms, based on spatially structured populations, following coarse-grained (Island Model) and fine-grained (Neighborhood Model) strategies. A distributed physical implementation, under the PVM environment, running in a local network, is described.Fundação para a Ciência e Tecnologia - PRAXIS/P/EEI/13096/98

Transcriptomes analysis of Aeromonas molluscorum Av27 cells exposed to tributyltin (TBT) : unravelling the effects from the molecular level to the organism

This work was supported by FCOMP-01-0124-FEDER-010630 project, co-funded by FEDER, European Funds through COMPETE and FCT within project PEst-C/MAR/LA0017/2013. Andreia Cruz was funded by the PhD grant SFRH/BD/36047/2007 from FCT and by a post-doctoral grant (BPD/UI88/2886/2013), from the project “Sustainable Use of Marine Resources” - MARES (CENTRO-07-ST24-FEDER-002033), funded by QREN, Mais Centro - Programa Operacional Regional do Centro e UE/Fundo Europeu de Desenvolvimento Regional. Bioinformatics support was provided by Miguel Pinheiro from the Bioinformatics Unit at St Andrews University funded by a Wellcome Trust ISSF grant (097831/Z/11/Z).Aeromonas molluscorum Av27 cells were exposed to 0, 5 and 50 μM of TBT and the respective transcriptomes were obtained by pyrosequencing. Gene Ontology revealed that exposure to 5 μM TBT results in a higher number of repressed genes in contrast with 50 μM of TBT, where the number of over-expressed genes is greater. At both TBT concentrations, higher variations in gene expression were found in the functional categories associated with enzymatic activities, transport/binding and oxidation-reduction. A number of proteins are affected by TBT, such as the acriflavin resistance protein, several transcription-related proteins, several Hsps, ABC transporters, CorA and ZntB and other outer membrane efflux proteins, all of these involved in cellular metabolic processes, important to maintain overall cell viability. Using the STRING tool, several proteins with unknown function were related with others involved in degradation processes, such as the pyoverdine chromophore biosynthetic protein, that has been described as playing a role in the Sn-C cleavage of organotins. This approach has allowed a better understanding of the molecular effects of exposure of bacterial cells to TBT. Furthermore it contributes to the knowledge of the functional genomic aspects of bacteria exposed to this pollutant. Furthermore, the transcriptomic data gathered, and now publically available, constitute a valuable resource for comparative genome analysis.Publisher PDFPeer reviewe

Durum wheat diversity for heat stress tolerance during inflorescence emergence is correlated to TdHSP101C expression in early developmental stages

Research articleThe predicted world population increase along with climate changes threatens sustainable agricultural supply in the coming decades. It is therefore vital to understand crops diversity associated to abiotic stress response. Heat stress is considered one of the major constrains on crops productivity thus it is essential to develop new approaches for a precocious and rigorous evaluation of varietal diversity regarding heat tolerance. Plant cell membrane thermostability (CMS) is a widely used method for wheat thermotolerance assessment although its limitations require complementary solutions. In this work we used CMS assay and explored TdHSP101C genes as an additional tool for durum wheat screening. Genomic and transcriptomic analyses of TdHSP101C genes were performed in varieties with contrasting CMS results and further correlated with heat stress tolerance during fertilization and seed development. Although the durum wheat varieties studied presented a very high homology on TdHSP101C genes (>99%) the transcriptomic assessment allowed the discrimination between varieties with good CMS results and its correlation with differential impacts of heat treatment during inflorescence emergence and seed development on grain yield. The evidences here reported indicate that TdHSP101C transcription levels induced by heat stress in fully expanded leaves may be a promising complementary screening tool to discriminate between durum wheat varieties identified as thermotolerant through CMSinfo:eu-repo/semantics/publishedVersio

Electrospun Polycaprolactone/Aloe Vera_Chitosan Nanofibrous Asymmetric Membranes Aimed for Wound Healing Applications

Today, none of the wound dressings available on the market is fully capable of reproducing all the features of native skin. Herein, an asymmetric electrospun membrane was produced to mimic both layers of skin. It comprises a top dense layer (manufactured with polycaprolactone) that was designed to provide mechanical support to the wound and a bottom porous layer (composed of chitosan and Aloe Vera) aimed to improve the bactericidal activity of the membrane and ultimately the healing process. The results obtained revealed that the produced asymmetric membranes displayed a porosity, wettability, as well as mechanical properties similar to those presented by the native skin. Fibroblast cells were able to adhere, spread, and proliferate on the surface of the membranes and the intrinsic structure of the two layers of the membrane is capable of avoiding the invasion of microorganisms while conferring bioactive properties. Such data reveals the potential of these asymmetric membranes, in the near future, to be applied as wound dressings

Unravelling the hidden inter and intra-varietal diversity of durum wheat commercial varieties used in Portugal

Short CommunicationAssessing durum wheat genomic diversity is crucial in a changing environmental particularly in the Mediterranean region where it is largely used to produce pasta. Durum wheat varieties cultivated in Portugal and previously assessed regarding thermotolerance ability were screened for the variability of coding sequences associated with technological traits and repetitive sequences. As expected, reduced variability was observed regarding low molecular weight glutenin subunits (LMW-GS) but a specific LMW-GS allelic form associated with improved pasta-making characteristics was absent in one variety. Contrastingly, molecular markers targeting repetitive elements like microsatellites and retrotransposons – Inter Simple Sequence Repeat (ISSR) and Inter Retrotransposons Amplified Polymorphism (IRAP) – disclosed significant inter and intra-varietal diversity. This high level of polymorphism was revealed by the 20 distinct ISSR/IRAP concatenated profiles observed among the 23 individuals analysed. Interestingly, median joining networks and PCoA analysis grouped individuals of the same variety and clustered varieties accordingly with geographical origin. Globally, this work demonstrates that durum wheat breeding strategies induced selection pressure for some relevant coding sequences while maintaining high levels of genomic variability in non-coding regions enriched in repetitive sequencesinfo:eu-repo/semantics/publishedVersio

Positive Psychological Capital as a Predictor of Satisfaction With the Fly-In Fly-Out Model

The flexibility of markets and international agreements have lured a growing number of companies to expand their business beyond frontiers in search for new markets and a bigger business network. Specifically, expatriates became keystones to implant and promote the so desired expansion into international markets, Particularly, Fly-in flyout (FIFO) flexpatriates. Although FIFO work practices are widely used, little is known about how to promote these professionals’ perceived job satisfaction (JS) across the course of their work cycles. That is why the goal of our research is to test the positive psychological capital (PsyCap) applicability to Portuguese FIFO flexpatriates. In the midst of the positive psychology theories, Luthans et al. (2007b) underline that workers are the psychological capital of any organization. Therefore, the development of the PsyCap becomes crucial and also contributes to the promotion of JS, nowadays a construct intertwined with well-being. As such, we developed and applied a HERO– (hope, self-efficacy, resilience, and optimism)–micro-intervention in order to assess whether it moderated the relationship between a FIFO flexpatriates PsyCap and their JS. The research took place over three distinct moments, both PsyCap and JS were measured before and after the HERO micro-intervention, and again 3 months later. The data collected shows that a positive correlation exists between FIFO flexpatriates PsyCap and JS. Moreover, our results pointed out that the micro-intervention enhanced FIFO flexpatriates PsyCap, and also showed that this increase lasted over (at least) 3 months.info:eu-repo/semantics/publishedVersio

A responsabilidade civil médica na especialidade de obstetrícia : o reconhecimento da violência obstétrica em Portugal

A presente dissertação destina-se ao estudo do tema da violência obstétrica, tomando por base os resultados de estudos nacionais e internacionais. Estamos perante um fenómeno atual que também ocorre nos hospitais portugueses, onde são realizados atos sem consentimento prévio e em violação das leges artis. Pretende-se analisar o tema à luz da responsabilidade civil médica, tanto contratual como extracontratual, fazendo ainda menção à possibilidade de legislação especifica sobre o tema que permita uma maior proteção das grávidas no momento do parto.The following dissertation proposes an analysis of the theme of obstetric violence, going from the result of national and international studies. It is an ongoing phenomenon that occurs at the national hospitals, where there are practices done without the informed consent and in violation of leges artis. This study aims to analysis within the scope of medical civil liability, contractual and extracontractual. Touching also on the possibility of specific legislation about this topic that seeks further protection of pregnant women during childbirth