Phreddie\u27s great adventure

Phreddie\u27s great adventur

There or not there? A multidisciplinary review and research agenda on the impact of transparent barriers on human perception, action, and social behavior

Contains fulltext : 145066.pdf (publisher's version ) (Open Access)Through advances in production and treatment technologies, transparent glass has become an increasingly versatile material and a global hallmark of modern architecture. In the shape of invisible barriers, it defines spaces while simultaneously shaping their lighting, noise, and climate conditions. Despite these unique architectural qualities, little is known regarding the human experience with glass barriers. Is a material that has been described as being simultaneously there and not there from an architectural perspective, actually there and/or not there from perceptual, behavioral, and social points of view? In this article, we review systematic observations and experimental studies that explore the impact of transparent barriers on human cognition and action. In doing so, the importance of empirical and multidisciplinary approaches to inform the use of glass in contemporary architecture is highlighted and key questions for future inquiry are identified.17 p

Mutational processes in Dictyostelium discoideum: How mutations affect social behaviors and fitness

Part I. Mutation is the most important biological force as it generates the variation that drives evolution and may play an important role in maintaining social structure in the social amoeba Dictyostelium discoideum. Using mutation accumulation lines of the social amoeba, I estimated the rate and degree of mutational effects on the social ability to form spores in chimeras by mixing equal proportions of cells of the ancestral clone with a mutated line and determining if the resultant spore proportion differs. Through the use of assays measuring growth, migration ability, and rates of spore germination, I assessed the fitness effects of mutation. In agreement with evidence that the majority of mutations are deleterious, I have found that the ability to get into the reproductive spores is diminished following mutation accumulation. Measuring growth rates on the selective medium revealed that approximately half of the lines showing a significant deviation from the ancestor have increased growth rates, possibly indicating the presence of beneficial mutations, while growth in a non-selective medium resulted in a loss of fitness. Additionally, spore germination decreased in lines with an abundance of mutations. Part II. Restriction Enzyme Mediated Integration (REMI) is a method of transformation that generates tagged mutations. We employed the REMI mutants to select for cheaters by competing pools of mutants over many generations, allowing the lines to fruit each time. We plated out high densities of spores in order to facilitate the lines bypassing the vegetative cycle but still allowing the social cycle. This process was repeated 20 times. At the end of this process, the frequency of each line was assessed and each line was sequenced to identify the genes that were affected by REMI mutagenesis. Once we had obligate cheaters, we assessed fitness in a variety of ways: axenic growth and growth on bacteria, rate of spore germination, and distance traveled by migrating slugs. We then looked for a correlation between cheating and fitness. We expect to see a tradeoff between the ability to preferentially produce spores in chimeric mixtures and other aspects of fitness

Frances H. Middlemist Interview, March 13, 1989

Interview with Frances H. Middlemist. This interview hasn\u27t been transcribed yet, but an index of the audio is available.https://scholarworks.umt.edu/educatorsmontana_interviews/1026/thumbnail.jp