Enhancement of Aedes albopictus collections by ovitrap and sticky adult trap

Abstract Background: In the last decades, Aedes albopictus has become an increasing public health threat in tropical as well as in more recently invaded temperate areas due to its capacity to transmit several human arboviruses, among which Dengue, Chikungunya and Zika. Enhancing the efficiency of currently used collection approaches, such as ovitraps and sticky traps, is desirable for optimal monitoring of the species abundance, for assessment of the risk of arbovirus transmission and for the optimisation of control activities. Findings: Two sets of 4 × 4 Latin-square experiments were carried out in Tirana (Albania) to test whether modifications in ovitrap shape and size and in oviposition substrate would increase collections of Ae. albopictus eggs and whether hay-infusion would increase adult catches by sticky trap. Generalized Linear Mixed Models with negative binomial error distribution were carried out to analyse the data. Cylindrical ovitraps lined with germination paper yielded significantly higher egg catches than those exploiting either the (commonly used) wooden paddles or floating polystyrene blocks as oviposition substrates. No difference was observed between cylindrical and conical shaped ovitraps. Ovitraps and sticky traps baited with hay infusion yielded significantly higher egg and adult catches than un-baited ones. A significant relationship between ovitrap and sticky trap catches was observed both in the absence and in the presence of attractants, with ovitrap catches increasing more than sticky trap catches at increasing adult female densities. Conclusions: This study provides grounds for optimisation of ovitraps and sticky traps as monitoring tools for Ae. albopictus by (i) supporting use of germination paper as most appropriate oviposition substrate; (ii) suggesting the possible use of stackable conical ovitraps for large scale monitoring; (iii) confirming the use of hay-infusion to increase egg catches in ovitraps, and showing that hay-infusion also significant increases adult catches by sticky traps

Forecasting the spatial and seasonal dynamic of Aedes albopictus oviposition activity in Albania and Balkan countries

The increasing spread of the Asian tiger mosquito, Aedes albopictus, in Europe and US raises public health concern due to the species competence to transmit several exotic human arboviruses, among which dengue, chikungunya and Zika, and urges the development of suitable modeling approach to forecast the spatial and temporal distribution of the mosquito. Here we developed a dynamical species distribution modeling approach forecasting Ae. albopictus eggs abundance at high spatial (0.01 degree WGS84) and temporal (weekly) resolution over 10 Balkan countries, using temperature times series of Modis data products and altitude as input predictors. The model was satisfactorily calibrated and validated over Albania based observed eggs abundance data weekly monitored during three years. For a given week of the year, eggs abundance was mainly predicted by the number of eggs and the mean temperature recorded in the preceding weeks. That is, results are in agreement with the biological cycle of the mosquito, reflecting the effect temperature on eggs spawning, maturation and hatching. The model, seeded by initial egg values derived from a second model, was then used to forecast the spatial and temporal distribution of eggs abundance over the selected Balkan countries, weekly in 2011, 2012 and 2013. The present study is a baseline to develop an easy-handling forecasting model able to provide information useful for promoting active surveillance and possibly prevention of Ae. albopictus colonization in presently non-infested areas in the Balkans as well as in other temperate regions

Altitudinal Variations In Wing Morphology Of Aedes Albopictus (Diptera, Culicidae) In Albania, The Region Where It Was First Recorded In Europe

The rapid spread and settlement of Aedes albopictus mosquitoes across at least 28 countries in Europe, as well as several countries in Asia Minor, the Middle East and Africa, has made it one of the most invasive species of all time. Even though the biology of Ae. albopictus in its native tropical environment has been documented for a long time, the biology and ecology of this species in newly colonized temperate environments remain poorly known despite its important role as a vector for about twenty arboviruses. In this context, the main goals of this work were to investigate Ae. albopictus phenotypic variations at a local scale in Albania, the country where Ae. albopictus was first recorded in Europe, and to determine if its phenotypes could be affected by altitude. Analysis of Ae. albopictus wing phenotypes was performed using a geometric morphometric approach. We observed shape and size variations among altitudinal populations of Ae. albopictus. Differences of wing phenotypes were highlighted between altitude groups for male and female mosquitoes. The phenotypic variations observed in Ae. albopictus between altitudinal groups indicated these populations are exposed to environmental and ecological pressures. These results suggest the presence of phenotypic plasticity in this species.PubMedWo

The current status of phlebotomine sand flies in Albania and incrimination of Phlebotomus neglectus (Diptera, Psychodidae) as the main vector of Leishmania infantum.

The incidence of visceral leishmaniasis (VL) in Albania is higher than in other countries of southern Europe, however the role of local sand fly species in the transmission of Leishmania infantum was not addressed conclusively. In 2006, a country-wide collection of sand flies performed in 14 sites selected based on recent occurrence of VL cases showed that Phlebotomus neglectus was by far the most prevalent species (95.6%). Furthermore, 15% of pools made from 422 P. neglectus females tested positive for Leishmania sp. genomic DNA. In the same year, Culicoides trapping was performed for bluetongue disease surveillance in 91 sites of southern Albania, targeting livestock farms regardless recent occurrence of VL in the surveyed areas. In 35 sites where sand flies were collected along with midges, Phlebotomus perfiliewi was the most prevalent among the Phlebotomus species identified, however search for leishmanial DNA in females of this species was unsuccessful. In 2011, sand flies were trapped in 4 sites of north Albania characterized by high VL incidence, and females were dissected to search for Leishmania infections. Both P. neglectus and P. tobbi were collected at high densities. Two positive specimens were detected from a sample of 64 P. neglectus trapped in one site (3.1%). Parasites were successfully cultured from one specimen and characterized as belonging to Leishmania infantum zymodeme MON-1, the only zymodeme so far identified as the agent of human and canine leishmaniasis in the country. Altogether our studies indicate that P. neglectus is the main leishmaniasis vector in Albania

All carnivores are not equal in the rural people's view. Should we develop conservation plans for functional guilds or individual species in the face of conflicts?

We tested differences in attitudes towards bears, wolves and lynx among the rural public in Albania and Macedonia through information collected from a questionnaire survey (n = 759). Wolves were the species with the least positive attitudes among the rural public and had the lowest support for conservation compared with bears and lynx. In addition, conflict perception of wolves was higher than for bears and lynx. We argue that, based on species specific differences in public attitudes, conservation initiatives and management plans for large carnivores should deal with wolves separately from bears and lynx, as lower public support for wolves might jeopardise the conservation of the two other large carnivores. Bears and lynx can be potentially treated together in conservation initiatives based on the similar levels of public support for conservation, however, from a conflict-management point of view, all three species need to be addressed separately

The current status of phlebotomine sand flies in Albania and incrimination of <i>Phlebotomus neglectus</i> (Diptera, Psychodidae) as the main vector of <i>Leishmania infantum</i> - Fig 2

<p><b>Sand fly collection sites (A and B).</b> Map showing: (A) 2006 sampling sites targeting foci of recent VL transmission and <i>Culicoides</i> midges monitoring in the frame of a bluetongue-disease surveillance program (see text for the sampling frame design); (B) 2011 sampling sites targeting foci of recent VL transmission.</p

Albanian <i>Leishmania</i> isolates identified from 1988 through 2012 by MultiLocus Enzyme Electrophoresis (MLEE) or PCR-restriction fragments length polymorphism of ribosomal internal transcribed spacer-1 (ITS-1 PCR-RFLP).

<p>Albanian <i>Leishmania</i> isolates identified from 1988 through 2012 by MultiLocus Enzyme Electrophoresis (MLEE) or PCR-restriction fragments length polymorphism of ribosomal internal transcribed spacer-1 (ITS-1 PCR-RFLP).</p

Prevalence and sand fly species identified by county and district in the 2006 country-wide study targeting VL endemic sites (Study 1).

<p>Prevalence and sand fly species identified by county and district in the 2006 country-wide study targeting VL endemic sites (Study 1).</p

<i>Leishmania</i> infections detected in wild-caught sand flies in sites of Lezhë district, September 2011 (Study 3).

<p><i>Leishmania</i> infections detected in wild-caught sand flies in sites of Lezhë district, September 2011 (Study 3).</p