60 research outputs found
Nucleotide Sequence of an Arabidopsis thaliana Turgor-Responsive TMP-B cDNA Clone Encoding Transmembrane Protein with a Major Intrinsic Protein Motif
Building promoter aware transcriptional regulatory networks using siRNA perturbation and deepCAGE
Perturbation and time-course data sets, in combination with computational approaches, can be used to infer transcriptional regulatory networks which ultimately govern the developmental pathways and responses of cells. Here, we individually knocked down the four transcription factors PU.1, IRF8, MYB and SP1 in the human monocyte leukemia THP-1 cell line and profiled the genome-wide transcriptional response of individual transcription starting sites using deep sequencing based Cap Analysis of Gene Expression. From the proximal promoter regions of the responding transcription starting sites, we derived de novo binding-site motifs, characterized their biological function and constructed a network. We found a previously described composite motif for PU.1 and IRF8 that explains the overlapping set of transcriptional responses upon knockdown of either factor
Cloning the interferon regulatory factor 1 gene in lungfish (Protopterus annectens) and its molecular evolution among sarcopterygians
Physiological evidence for diversification of IFNĪ±- and IFNĪ²-mediated response programs in different autoimmune diseases
Interactions with Commensal and Pathogenic Bacteria Induce HIV-1 Latency in Macrophages through Altered Transcription Factor Recruitment to the Long Terminal Repeat
Induction of Samhd1 by interferon gamma and lipopolysaccharide in murine macrophages requiresĀ IRF1
IFN-Stimulated Gene 15 Is Synergistically Activated Through Interactions Between the Myelocyte/Lymphocyte-Specific Transcription Factors, PU.1, IFN Regulatory Factor-8/IFN Consensus Sequence Binding Protein, and IFN Regulatory Factor-4: Characterization of a New Subtype of IFN-Stimulated Response Element
A model of the three-dimensional structure of human interferon responsive factor 1 and its modifications upon phosphorylation or phosphorylation-mimicking mutations
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