Mammals of Spanish Peaks State Wildlife Area, Colorado

Habitats and relative abundance of small mammals in the Spanish Peaks State Wildlife Area in western Las Animas Co., Colorado, were assessed. A total of 821 mammals of 20 species was collected in 2750 trapnights between 31 May and 19 August of 1981. Eight additional species of mammals were observed on the wildlife area but not documented. The deer mouse (Peromyscus maniculatus) and rock mouse (P. difficilis) were the most abundant small mammals, accounting for 63 and 16%, respectively, of the total catch. The deer mouse was the only species caught in every habitat sampled. Abert\u27s squirrel (Sciurus abert,) previously has not been recorded in Colorado from as far southeast as the Spanish Peaks State Wildlife Area. The mammalian fauna of Colorado has been the subject of numerous studies since the late 1800s. Early studies were by Cockerell (1890), Warren (1906), Cary (1911), and Warren (1942, published posthumously). More recent investigations include the book by Lechleitner (1969), the exhaustive compilation by Armstrong (1972), and the latilong surveys by Bissell (1978, 1982). In spite of these studies, several regions of Colorado remain poorly represented in collections. Armstrong (1972) noted that certain poorly surveyed parts of the western two-thirds of the state appear to me to be ... significant from the standpoint of zoogeography. Western Las Animas County--the Park Plateau and Culebra Range [of the Sangre de Cristo Mountains]--is such an area. In a later publication, Armstrong et al. (1973) noted that additional field investigations were needed in the vicinity of the Wet Mountains, a biogeographically important region where several species of grassland mammals reach altitudinal extremes and certain southern species reach northern limits. With this in mind, the Colorado Division of Wildlife initiated fieldwork in the Spanish Peaks State Wildlife Area to sample the vertebrate fauna in general and to assess habitat associations and relative abundance of small mammals in particular. This report documents the mammals collected and observed in the Spanish Peaks State Wildlife Area during summer of 1981 and summarizes biological data on mammals obtained at that time.https://scholars.fhsu.edu/fort_hays_studies_series/1067/thumbnail.jp

Multiple Lines of Evidence Risk Assessment of Terrestrial Passerines Exposed to PCDFs and PCDDs in the Tittabawassee River Floodplain, Midland, Michigan, USA

A site-specific multiple lines of evidence risk assessment was conducted for house wrens (Troglodytes aedon) and eastern bluebirds (Sialia sialis) along the Tittabawassee River downstream of Midland, Michigan, where concentrations of polychlorinated dibenzofurans (PCDFs) and polychlorinated dibenzo-p-dioxins (PCDDs) in flood-plain soils and sediments are greater compared to upstream areas and some of the greatest anywhere in the world. Lines of evidence supporting the population-level assessment endpoints included site-specific dietary- and tissue-based exposure assessments and population productivity measurements during breeding seasons 2005–2007. While a hazard assessment based on site-specific diets suggested that populations residing in the downstream floodplain had the potential to be affected, concentrations in eggs compared to appropriate toxicity reference values (TRVs) did not predict a potential for population-level effects. There were no significant effects on reproductive success of either species. The most probable cause of the apparent difference between the dietary- and tissue-based exposure assessments was that the dietary-based TRVs were overly conservative based on intraperitoneal injections in the ring-necked pheasant. Agreement between the risk assessment based on concentrations of PCDFs and PCDDs in eggs and reproductive performance in both species supports the conclusion of a small potential for population-level effects at this site

Efficient Dielectrophoretic Patterning of Embryonic Stem Cells in Energy Landscapes Defined by Hydrogel Geometries

In this study, we have developed an integrated microfluidic platform for actively patterning mammalian cells, where poly(ethylene glycol) (PEG) hydrogels play two important roles as a non-fouling layer and a dielectric structure. The developed system has an embedded array of PEG microwells fabricated on a planar indium tin oxide (ITO) electrode. Due to its dielectric properties, the PEG microwells define electrical energy landscapes, effectively forming positive dielectrophoresis (DEP) traps in a low-conductivity environment. Distribution of DEP forces on a model cell was first estimated by computationally solving quasi-electrostatic Maxwell’s equations, followed by an experimental demonstration of cell and particle patterning without an external flow. Furthermore, efficient patterning of mouse embryonic stem (mES) cells was successfully achieved in combination with an external flow. With a seeding density of 107 cells/mL and a flow rate of 3 μL/min, trapping of cells in the microwells was completed in tens of seconds after initiation of the DEP operation. Captured cells subsequently formed viable and homogeneous monolayer patterns. This simple approach could provide an efficient strategy for fabricating various cell microarrays for applications such as cell-based biosensors, drug discovery, and cell microenvironment studies

The genome of the sea urchin Strongylocentrotus purpuratus

We report the sequence and analysis of the 814-megabase genome of the sea urchin Strongylocentrotus purpuratus, a model for developmental and systems biology. The sequencing strategy combined whole-genome shotgun and bacterial artificial chromosome (BAC) sequences. This use of BAC clones, aided by a pooling strategy, overcame difficulties associated with high heterozygosity of the genome. The genome encodes about 23,300 genes, including many previously thought to be vertebrate innovations or known only outside the deuterostomes. This echinoderm genome provides an evolutionary outgroup for the chordates and yields insights into the evolution of deuterostomes

Morphological characterization of bushy cells and their inputs in the laboratory mouse (Mus musculus) anteroventral cochlear nucleus.

PMC3753269Spherical and globular bushy cells of the AVCN receive huge auditory nerve endings specialized for high fidelity neural transmission in response to acoustic events. Recent studies in mice and other rodent species suggest that the distinction between bushy cell subtypes is not always straightforward. We conducted a systematic investigation of mouse bushy cells along the rostral-caudal axis in an effort to understand the morphological variation that gives rise to reported response properties in mice. We combined quantitative light and electron microscopy to investigate variations in cell morphology, immunostaining, and the distribution of primary and non-primary synaptic inputs along the rostral-caudal axis. Overall, large regional differences in bushy cell characteristics were not found; however, rostral bushy cells received a different complement of axosomatic input compared to caudal bushy cells. The percentage of primary auditory nerve terminals was larger in caudal AVCN, whereas non-primary excitatory and inhibitory inputs were more common in rostral AVCN. Other ultrastructural characteristics of primary auditory nerve inputs were similar across the rostral and caudal AVCN. Cross sectional area, postsynaptic density length and curvature, and mitochondrial volume fraction were similar for axosomatic auditory nerve terminals, although rostral auditory nerve terminals contained a greater concentration of synaptic vesicles near the postsynaptic densities. These data demonstrate regional differences in synaptic organization of inputs to mouse bushy cells rather than the morphological characteristic of the cells themselves.JH Libraries Open Access Fun