Eddy Impacts on the Florida Current

The Gulf Stream in the Atlantic carries warm water northwards and forms both the return closure of the subtropical gyre as well as the upper limb of the meridional overturning circulation. Recent time series recorded east of the Bahamas at 26°N indicate that from May 2009 to April 2011, in contrast with past observations, the northward flowing Antilles Current covaried with the Gulf Stream in the Florida Straits—the Florida Current—even though the Florida and Antilles Currents are separated by banks and islands spanning 150?km. The peak-to-trough amplitude of transport variations during this period was 15?×?106?m3?s?1 for the Florida Current and 12?×?106?m3?s?1 for the Antilles Current, at time scales of 50?days to a year. From satellite observations, we show that the fluctuations in both the Florida and Antilles Currents between May 2009 and April 2011 are driven by eddy activity east of the Bahamas. Since the Florida Current time series is a critical time series for the state of the oceans, and often compared to climate models, this newly identified source of variability needs careful consideration when attributing the variability of the Florida Current to changes in the larger-scale circulations (e.g., gyre and overturning) or wind forcing.<br/

Spatial distribution of ions in a linear octopole radio-frequency ion trap in the space-charge limit

We have explored the spatial distribution of an ion cloud trapped in a linear octopole radio-frequency (rf) ion trap. The two-dimensional distribution of the column density of stored silver dimer cations was measured via photofragment-ion yields as a function of the position of the incident laser beam over the transverse cross section of the trap. The profile of the ion distribution was found to be dependent on the number of loaded ions. Under high ion-loading conditions with a significant space-charge effect, ions form a ring profile with a maximum at the outer region of the trap, whereas they are localized near the center axis region at low loading of the ions. These results are explained quantitatively by a model calculation based on equilibrium between the space-charge-induced potential and the effective potential of the multipole rf field. The maximum adiabaticity parameter \eta_max is estimated to be about 0.13 for the high ion-density condition in the present octopole ion trap, which is lower than typical values reported for low ion densities; this is probably due to additional instability caused by the space charge.Comment: 8 pages, 5 figure

Implications of changing El Niño patterns for biological dynamicsin the equatorial Pacific Ocean

El Niño events are known to strongly affect biological production and ecosystem structure in the tropical Pacific. Understanding and predicting biological processes in this area are hampered because the existing in situ observing system focuses primarily on physical measurements and does not observe key biological parameters; the only high spatial and temporal resolution biology-related observations are from the global array of ocean color satellites which provide an estimate of surface chlorophyll concentrations only. Since the1990s, an apparent shift of the El Niño maximum sea-surface temperature (SST) warm anomaly from the eastern to the central equatorial Pacific has frequently been observed. Satellite observations show significant changes in chlorophyll-a(Chl-a), new production (NP) and total primary production(PP) in the equatorial Pacific associated with these new central Pacific (CP) El Niño events (also called El Niño Modoki) relative to eastern Pacific El Niños. During CP-El Niños, NP, Chl-a and PP in the central basin are depressed relative to EP-El Niños and lower values of Chl-a and PP coincide spatially with higher SST in the central Pacific. While surface Chl-a, and integrated NP and PP over the entire equatorial band, decrease during both CP and EP-El Niños, the magnitude of this decrease seems to depend more on the intensity than type of event. The changing spatial patterns have significant implications for equatorial biological dynamics if, as has been suggested, CP-El Niños increase in frequency in the future

Segmented filamentous bacteria-based treatment to elicit protection against Enterobacteriaceae in Layer chickens

IntroductionGut microbes like segmented filamentous bacteria (SFB) play a key role in gut maturation during early life, as demonstrated in humans and mice. Our previous study demonstrated oral inoculation of ileum-spores containing SFB to chickens after hatch increases early SFB gut colonization, which increases immune maturation and resistance to bacteria, like Salmonella, as tested in vitro; however, more studies are needed for treatment optimization and in vivo testing. The objectives of this study were to (1) test a treatment that includes both spores and filamentous SFB, (2) validate antimicrobial ability of the treatment in layer hens in vivo, and (3) elucidate its molecular mechanism.MethodsOne-day-old specific pathogen-free layers (n = 12 per group) were orally treated with either PBS (CON) or SFB-based treatment (SFB). At 4 days post-inoculation (DPI), both CON and SFB groups were orally challenged with Salmonella Typhimurium. Total Enterobacteriaceae and Salmonella were examined by plating and enumeration in feces at 7,10 and 14 dpi; and in the ileum, cecum, and spleen at 16 dpi in euthanized birds. The presence and levels of SFB were determined from ilea scrapings via microscopy and qPCR, respectively. Relative gene expression of host-derived antimicrobial peptides and cytokines in the distal ileum was determined by RT-qPCR.ResultsAt 10 and 14 dpi, a significant decrease in total Enterobacteriaceae was observed in the feces of the SFB group. At necropsy, the level of SFB was significantly higher in the SFB group than in the CON group, while a significant decrease in total Enterobacteriaceae and Salmonella was observed in the ceca of the SFB group. RT-qPCR revealed increased expression of β-defensin 14, and cytokines IL-10 and IFNγ.DiscussionThe introduction of SFB at hatch as a prophylactic treatment may benefit commercial partners as well as consumers by reducing the incidence of Enterobacteriaceae in food animals. Reduction of these bacteria in animals would, in turn, increase animal health, productivity, and safety for consumers. Studies to optimize the treatment for poultry industry applications are ongoing in our lab

Observed Ocean Bottom Temperature Variability at Four Sites in the Northwestern Argentine Basin: Evidence of Decadal Deep/Abyssal Warming Amidst Hourly to Interannual Variability During 2009–2019

Consecutive multiyear records of hourly ocean bottom temperature measurements are merged to produce new decade-long time series at four depths ranging from 1,360 to 4,757 m within the northwest Argentine Basin at 34.5°S. Energetic temperature variations are found at a wide range of time scales. All sites exhibit fairly linear warming trends of approximately 0.02–0.04°C per decade over the period 2009–2019, although the trends are only statistically different from zero at the two deepest sites at depths of ~4,500–4,800 m. Near-bottom temperatures from independent conductivity-temperature-depth profiles collected at these same locations every 6–24 months over the same decade show roughly consistent trends. Based on the distribution of spectral energies at the deepest sites and a Monte Carlo-style analysis, sampling at least once per year is necessary to capture the significant warming trends over this decade to within 50% error bars at a 95% confidence limit.Fil: Meinen, Christopher S.. National Ocean And Atmospheric Administration; Estados UnidosFil: Perez, Renellys C.. National Ocean And Atmospheric Administration; Estados UnidosFil: Dong, Shenfu. National Ocean And Atmospheric Administration; Estados UnidosFil: Piola, Alberto Ricardo. Ministerio de Defensa. Armada Argentina. Servicio de Hidrografía Naval; Argentina. Instituto Franco-Argentino sobre Estudios del Clima y sus Impactos; Argentina. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Campos, Edmo. Universidade de Sao Paulo; Brasil. American University Of Sharjah.; Emiratos Árabes Unido

A prototype system for observing the Atlantic Meridional Overturning Circulation - scientific basis, measurement and risk mitigation strategies, and first results

The Atlantic Meridional Overturning Circulation (MOC) carries up to one quarter of the global northward heat transport in the Subtropical North Atlantic. A system monitoring the strength of the MOC volume transport has been operating since April 2004. The core of this system is an array of moored sensors measuring density, bottom pressure and ocean currents. A strategy to mitigate risks of possible partial failures of the array is presented, relying on backup and complementary measurements. The MOC is decomposed into five components, making use of the continuous moored observations, and of cable measurements across the Straits of Florida, and wind stress data. The components compensate for each other, indicating that the system is working reliably. The year-long average strength of the MOC is 18.7±5.6 Sv, with wind-driven and density-inferred transports contributing equally to the variability. Numerical simulations suggest that the surprisingly fast density changes at the western boundary are partially linked to westward propagating planetary wave

Chimeric protein repair of laminin polymerization ameliorates muscular dystrophy phenotype

Mutations in laminin α2-subunit (Lmα2, encoded by LAMA2) are linked to approximately 30% of congenital muscular dystrophy cases. Mice with a homozygous mutation in Lama2 (dy2J mice) express a nonpolymerizing form of laminin-211 (Lm211) and are a model for ambulatory-type Lmα2-deficient muscular dystrophy. Here, we developed transgenic dy2J mice with muscle-specific expression of αLNNd, a laminin/nidogen chimeric protein that provides a missing polymerization domain. Muscle-specific expression of αLNNd in dy2J mice resulted in strong amelioration of the dystrophic phenotype, manifested by the prevention of fibrosis and restoration of forelimb grip strength. αLNNd also restored myofiber shape, size, and numbers to control levels in dy2J mice. Laminin immunostaining and quantitation of tissue extractions revealed increased Lm211 expression in αLNNd-transgenic dy2J mice. In cultured myotubes, we determined that αLNNd expression increased myotube surface accumulation of polymerization-deficient recombinant laminins, with retention of collagen IV, reiterating the basement membrane (BM) changes observed in vivo. Laminin LN domain mutations linked to several of the Lmα2-deficient muscular dystrophies are predicted to compromise polymerization. The data herein support the hypothesis that engineered expression of αLNNd can overcome polymerization deficits to increase laminin, stabilize BM structure, and substantially ameliorate muscular dystrophy

The fate of the Deep Western Boundary Current in the South Atlantic

The pathways of recently ventilated North Atlantic Deep Water (NADW) are part of the lower limb of the Atlantic Meridional Overturning Circulation (AMOC). In the South Atlantic these pathways have been the subject of discussion for years, mostly due to the lack of observations. Knowledge of the pathways of the AMOC in the South Atlantic is a first order prerequisite for understanding the fluxes of climatically important properties. In this paper, historical and new observations, including hydrographic and oxygen sections, Argo data, and chlorofluorocarbons (CFCs), are examined together with two different analyzes of the Ocean general circulation model For the Earth Simulator (OFES) to trace the pathway of the recently ventilated NADW through the South Atlantic. CLIVAR-era CFCs, oxygen and salinity clearly show that the strongest NADW pathway in the South Atlantic is along the western boundary (similar to the North Atlantic). In addition to the western boundary pathway, tracers show an eastward spreading of NADW between ~17 and 25°S. Analyzed together with the results of earlier studies, the observations and model output presented here indicate that after crossing the equator, the Deep Western Boundary Current (DWBC) transports water with the characteristics of NADW and a total volume transport of approximately 14Sv (1Sv=106m3s-1). It crosses 5°S as a narrow western boundary current and becomes dominated by eddies further south. When this very energetic eddying flow reaches the Vitória-Trindade Ridge (~20°S), the flow follows two different pathways. The main portion of the NADW flow continues along the continental shelf of South America in the form of a strong reformed DWBC, while a smaller portion, about 22% of the initial transport, flows towards the interior of the basin

Elaborating a coiledâ coilâ assembled octahedral protein cage with additional protein domains

De novo design of protein nanoâ cages has potential applications in medicine, synthetic biology, and materials science. We recently developed a modular, symmetryâ based strategy for protein assembly in which short, coiledâ coil sequences mediate the assembly of a protein building block into a cage. The geometry of the cage is specified by the combination of rotational symmetries associated with the coiledâ coil and protein building block. We have used this approach to design wellâ defined octahedral and tetrahedral cages. Here, we show that the cages can be further elaborated and functionalized by the addition of another protein domain to the free end of the coiledâ coil: in this case by fusing maltoseâ binding protein to an octahedral protein cage to produce a structure with a designed molecular weight of ~1.8 MDa. Importantly, the addition of the maltose binding protein domain dramatically improved the efficiency of assembly, resulting in ~ 60â fold greater yield of purified protein compared to the original cage design. This study shows the potential of using small, coiledâ coil motifs as offâ theâ shelf components to design MDaâ sized protein cages to which additional structural or functional elements can be added in a modular manner.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/146469/1/pro3497.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/146469/2/pro3497_am.pd