Characterization of Iranian grapevine cultivars using microsatellite markers

Sixty-two grapevine (Vitis spp.) accessions from Iran and the USA were characterized at 9 highly polymorphic microsatellite loci using fluorescent primers and a capillary electrophoresis fragment sizing system. The number of alleles observed per locus ranged from 4 to 16 and heterozygosity values ranged from 0.47 to 0.86. Genetic similarity was estimated for each pair of accessions as the proportion of shared alleles. A phenogram constructed from genetic dissimilarity values revealed three clusters, one each for table grapes, wine grapes and rootstocks. The phenogram also revealed three clonal sets (Askari, Bidane and Yaghoti) as well as some synonyms and homonyms among Iranian table grape cultivars.

Effective User Experience in Online Technical Communication Courses: Employing Multiple Methods within Organizational Contexts to Assess Usability

In teaching online technical communication courses, shaping an electronic interface requires extensive consideration of the user experience, both for students and for faculty members who design and teach the courses. Technical communication faculty members should provide strong examples of effective user experiences and should be leaders in making the interfaces of online learning management systems as usable as possible. Principles of usability designed for general web sites may or may not apply to learning management systems designed for educational purposes. In order to create effective online technical communication courses, one needs to consider both usability concerns and pedagogical concerns. To assess the usability and pedagogical effectiveness of online courses, faculty members may use indirect means such as heuristic analyses. In addition, they may use direct means such as usability testing, student feedback, and analytic tools. Each approach has advantages as well as limitations. Faculty members will gain the richest information through using multiple approaches. In assessing usability and pedagogical effectiveness, faculty members also need to consider the situational constraints and resources in their unique contexts. Understanding and adapting their approaches to use resources well and to work within constraints will benefit their abilities to enhance their student users' experiences with online courses

'Yamhill' hazelnut (OSU 542.102)

"'Yamhill' (OSU 542.102) was developed and evaluated by the hazelnut breeding program at Oregon State University, Corvallis, Oregon, and was released in January 2008. Trees of 'Yamhill' are completely resistant to eastern filbert blight (EFB) caused by Anisogramma anomala (Peck) E. Müller. Compared to 'Barcelona', trees of 'Yamhill' are smaller but very productive, and nuts mature at the same time as those of 'Santiam' and 'Gamma', approximately 10-15 days ahead of 'Barcelona'. Marketable nut yields exceed 'Barcelona', and kernels have few defects, although they are small and best suited for the kernel market."--P. 1.Reviewed August 2009. Facts and recommendations in this publication may no longer be valid. Please look for up-to-date information in the OSU Extension Catalog: http://extension.oregonstate.edu/catalo

Scientific writing: a randomized controlled trial comparing standard and on-line instruction

<p>Abstract</p> <p>Background</p> <p>Writing plays a central role in the communication of scientific ideas and is therefore a key aspect in researcher education, ultimately determining the success and long-term sustainability of their careers. Despite the growing popularity of e-learning, we are not aware of any existing study comparing on-line vs. traditional classroom-based methods for teaching scientific writing.</p> <p>Methods</p> <p>Forty eight participants from a medical, nursing and physiotherapy background from US and Brazil were randomly assigned to two groups (n = 24 per group): An on-line writing workshop group (on-line group), in which participants used virtual communication, google docs and standard writing templates, and a standard writing guidance training (standard group) where participants received standard instruction without the aid of virtual communication and writing templates. Two outcomes, manuscript quality was assessed using the scores obtained in Six subgroup analysis scale as the primary outcome measure, and satisfaction scores with Likert scale were evaluated. To control for observer variability, inter-observer reliability was assessed using Fleiss's kappa. A post-hoc analysis comparing rates of communication between mentors and participants was performed. Nonparametric tests were used to assess intervention efficacy.</p> <p>Results</p> <p>Excellent inter-observer reliability among three reviewers was found, with an Intraclass Correlation Coefficient (ICC) agreement = 0.931882 and ICC consistency = 0.932485. On-line group had better overall manuscript quality (p = 0.0017, SSQSavg score 75.3 ± 14.21, ranging from 37 to 94) compared to the standard group (47.27 ± 14.64, ranging from 20 to 72). Participant satisfaction was higher in the on-line group (4.3 ± 0.73) compared to the standard group (3.09 ± 1.11) (p = 0.001). The standard group also had fewer communication events compared to the on-line group (0.91 ± 0.81 vs. 2.05 ± 1.23; p = 0.0219).</p> <p>Conclusion</p> <p>Our protocol for on-line scientific writing instruction is better than standard face-to-face instruction in terms of writing quality and student satisfaction. Future studies should evaluate the protocol efficacy in larger longitudinal cohorts involving participants from different languages.</p

Chilling-Dependent Release of Seed and Bud Dormancy in Peach Associates to Common Changes in Gene Expression

Reproductive meristems and embryos display dormancy mechanisms in specialized structures named respectively buds and seeds that arrest the growth of perennial plants until environmental conditions are optimal for survival. Dormancy shows common physiological features in buds and seeds. A genotype-specific period of chilling is usually required to release dormancy by molecular mechanisms that are still poorly understood. In order to find common transcriptional pathways associated to dormancy release, we analyzed the chilling-dependent expression in embryos of certain genes that were previously found related to dormancy in flower buds of peach. We propose the presence of short and long-term dormancy events affecting respectively the germination rate and seedling development by independent mechanisms. Short periods of chilling seem to improve germination in an abscisic acid-dependent manner, whereas the positive effect of longer cold treatments on physiological dwarfing coincides with the accumulation of phenylpropanoids in the seed

Genetic diversity in hazelnut (Corylus avellana L.) cultivars from Black Sea countries assessed using SSR markers

P>European hazelnut (Corylus avellana L.) is an important crop in Turkey, Georgia and Azerbaijan, where cultivars were selected from the native vegetation. Accessions from Turkey have been assigned to the Black Sea group, and cultivars from Georgia and Azerbaijan have a similar phenotype. Genetic diversity was investigated in 88 accessions from these three countries and compared with cultivars from Spain and Italy using 12 microsatellite loci. A high level of genetic diversity (H(e) = 0.71, H(o) = 0.70) was observed in the Black Sea accessions. Six Turkish accessions in the US hazelnut collections were found to be synonyms of cultivars in the Turkish collection in Giresun. An unweighted pair-group method using arithmetic average dendrogram and principal component analysis of 109 unique accessions showed a tendency to form subgroups by country of origin, and high diversity within each subgroup. A moderate shift in allelic frequencies (F(ST) = 0.114-0.131) was seen between accessions from the Black Sea and the Spanish-Italian accessions. Simple sequence repeat analysis identified the putative parents of two Turkish cultivars.European hazelnut (Corylus avellana L.) is an important crop in Turkey, Georgia and Azerbaijan, where cultivars were selected from the native vegetation. Accessions from Turkey have been assigned to the Black Sea group, and cultivars from Georgia and Azerbaijan have a similar phenotype. Genetic diversity was investigated in 88 accessions from these three countries and compared with cultivars from Spain and Italy using 12 microsatellite loci. A high level of genetic diversity (He = 0.71, Ho = 0.70) was observed in the Black Sea accessions. Six Turkish accessions in the US hazelnut collections were found to be synonyms of cultivars in the Turkish collection in Giresun. An unweighted pair-group method using arithmetic average dendrogram and principal component analysis of 109 unique accessions showed a tendency to form subgroups by country of origin, and high diversity within each subgroup. A moderate shift in allelic frequencies (FST = 0.114–0.131) was seen between accessions from the Black Sea and the Spanish-Italian accessions. Simple sequence repeat analysis identified the putative parents of two Turkish cultivars