The Evolution of Oxygen and Magnesium in the Bulge and Disk of the Milky Way

We show that the Galactic bulge and disk share a similar, strong, decline in [O/Mg] ratio with [Mg/H]. The similarity of the [O/Mg] trend in these two, markedly different, populations suggests a metallicity-dependent modulation of the stellar yields from massive stars, by mass loss from winds, and related to the Wolf-Rayet phenomenon, as proposed by McWilliam & Rich (2004). We have modified existing models for the chemical evolution of the Galactic bulge and the solar neighborhood with the inclusion of metallicity-dependent oxygen yields from theoretical predictions for massive stars that include mass loss by stellar winds. Our results significantly improve the agreement between predicted and observed [O/Mg] ratios in the bulge and disk above solar metallicity; however, a small zero-point normalization problem remains to be resolved. The zero-point shift indicates that either the semi-empirical yields of Francois et al. (2004) need adjustment, or that the bulge IMF is not quite as flat as found by Ballero et al. (2007); the former explanation is preferred. Our result removes a previous inconsistency between the interpretation of [O/Fe] and [Mg/Fe] ratios in the bulge, and confirms the conclusion that the bulge formed more rapidly than the disk, based on the over-abundances of elements produced by massive stars. We also provide an explanation for the long-standing difference between [Mg/Fe] and [O/Fe] trends among disk stars more metal-rich than the sun.Comment: 22 pages including 5 figures. Submitted to the Astronomical Journa

Coordinates and 2MASS and OGLE identifications for all stars in Arp's 1965 finding chart for Baade's Window

Aims: We seek to provide 2MASS and OGLE identifications and coordinates for all stars in the finding chart published by Arp\,(1965). This chart covers the low extinction area around NGC 6522, also known as Baade's window, at coordinates (l,b)=(1.02,-3.92). Methods: A cross correlation, using numerical techniques, was performed between a scan of the original finding chart from Arp (1965) and 2MASS and OGLE-II images and stellar coordinates. Results: We provide coordinates for all stars in Arp's finding chart and 2MASS and OGLE identifications wherever possible. Two identifications in quadrant II do not appear in the original finding chart.Comment: 30 pages, accepted by A&A as a Research Not

The IMGT/HLA database

It is 14 years since the IMGT/HLA database was first released, providing the HLA community with a searchable repository of highly curated HLA sequences. The HLA complex is located within the 6p21.3 region of human chromosome 6 and contains more than 220 genes of diverse function. Of these, 21 genes encode proteins of the immune system that are highly polymorphic. The naming of these HLA genes and alleles and their quality control is the responsibility of the World Health Organization Nomenclature Committee for Factors of the HLA System. Through the work of the HLA Informatics Group and in collaboration with the European Bioinformatics Institute, we are able to provide public access to these data through the website http://www.ebi.ac.uk/imgt/hla/. Regular updates to the website ensure that new and confirmatory sequences are dispersed to the HLA community and the wider research and clinical communities. This article describes the latest updates and additional tools added to the IMGT/HLA project

Characterisation and application of a bovine U6 promoter for expression of short hairpin RNAs

BackgroundThe use of small interfering RNA (siRNA) molecules in animals to achieve double-stranded RNA-mediated interference (RNAi) has recently emerged as a powerful method of sequence-specific gene knockdown. As DNA-based expression of short hairpin RNA (shRNA) for RNAi may offer some advantages over chemical and in vitro synthesised siRNA, a number of vectors for expression of shRNA have been developed. These often feature polymerase III (pol. III) promoters of either mouse or human origin.ResultsTo develop a shRNA expression vector specifically for bovine RNAi applications, we identified and characterised a novel bovine U6 small nuclear RNA (snRNA) promoter from bovine sequence data. This promoter is the putative bovine homologue of the human U6-8 snRNA promoter, and features a number of functional sequence elements that are characteristic of these types of pol. III promoters. A PCR based cloning strategy was used to incorporate this promoter sequence into plasmid vectors along with shRNA sequences for RNAi. The promoter was then used to express shRNAs, which resulted in the efficient knockdown of an exogenous reporter gene and an endogenous bovine gene.ConclusionWe have mined data from the bovine genome sequencing project to identify a functional bovine U6 promoter and used the promoter sequence to construct a shRNA expression vector. The use of this native bovine promoter in shRNA expression is an important component of our future development of RNAi therapeutic and transgenic applications in bovine species.<br /

On our side: A grounded theory of manager support in a prison setting

This project explores the challenges of managing in times of organisational stress. The New Zealand department of corrections is facing multiple pressures which are only set to increase including: financial strictures as government funding is being highly scrutinised, greater demand as inmate numbers increase, and reduced capabilities as many staff lack experience. A grounded theory in a case study setting (three prisons in Christchurch, New Zealand) was undertaken utilising repertory grid and semi-structured interviews to explore the ways in which managers cope during times of such stress. A total of 11 interviews were conducted. In the case, I considered what differentiates effective managers from those who appear less able to cope. It was found that effective managers are those who are able to build trust and respect with their constituents. When staff trust and respect their managers it is because they feel valued and perceive their manager to be on their side; they are then willing to reciprocate. Positive regard, demonstrations of support, and leading by example were found to be key factors leading to being perceived as being on the staff’s side. The links between trust, respect and performance along with the valuation of staff wellbeing were examined

Two distinct halo populations in the solar neighborhood. II. Evidence from stellar abundances of Mn, Cu, Zn, Y, and Ba

A previous study (Nissen & Schuster 2010) of 94 dwarf stars with -1.6 < [Fe/H] < -0.4 has revealed the existence of two distinct halo populations with a systematic difference in [alpha/Fe] at a given metallicity. In continuation of that work, abundances of Mn, Cu, Zn, Y, and Ba are determined for the same sample of stars. Equivalent widths of atomic lines are measured from high resolution VLT/UVES and NOT/FIES spectra and used to derive precise abundance ratios from an LTE analysis based on MARCS model atmospheres. Systematic differences between the `high-alpha' and `low-alpha' halo populations are found for [Cu/Fe], [Zn/Fe], and [Ba/Y], whereas there is no significant difference in the case of [Mn/Fe]. At a given metallicity, [Cu/Fe] shows a large scatter that is closely correlated with a corresponding scatter in [Na/Fe] and [Ni/Fe]. The metallicity trends of [Cu/Fe], [Zn/Fe], and [Ba/Y] can be explained from existing nucleosynthesis calculations if the high-alpha stars formed in regions with such a high star formation rate that only massive stars and Type II supernovae contributed to the chemical enrichment. The low-alpha stars, on the other hand, most likely originate from systems with a slower chemical evolution, characterized by additional enrichment from Type Ia supernovae and low-mass AGB stars.Comment: Accepted for publication in A&

Studying functions on coral reefs : past perspectives, current conundrums, and future potential

This work was funded by the Australian Research Council (DRB; grant number FL190100062).Function-based studies have opened a new chapter in our understanding of coral reefs. Unfortunately, we are opening this chapter as the world’s reefs rapidly transform. In this context, one of the most important roles of function-based studies is to inform coral reef conservation. At this critical juncture, we have a chance to reflect on where we have come from, and where we are going, in coral reef functional ecology, with specific consideration of what this means for our approaches to conserving reefs. As focal examples, we examine the role of corals on reefs, and the practice of culling crown-of-thorns starfish, from a functional perspective. We also consider how the papers in this special issue build on our current understanding. Ultimately, we highlight how robust scientific investigation, based on an understanding of ecosystem functions, will be key in helping us navigate reefs through the current coral reef crisis.Peer reviewe

Real-time buffer gas pressure tuning in a micro-machined vapor cell

We demonstrate a controllable depletion of the nitrogen buffer gas pressure in a micro-machined cesium (Cs) vapor cell from the dynamic heating of an alkali dispenser pill. When the alkali source is laser activated, the gettering compounds within the alkali pill dispenser reduce the nitrogen (N$_2$) content from the vapor for fine-tuning of the alkali to buffer gas pressure ratio. Additionally, we decrease the buffer gas pressure below 100$\,$mTorr to evaluate the presence of other potential broadening mechanisms. Real-time control of the gas pressure ratio in the vapor cell will have notable benefits for refining atomic sensor performance and provide a routine to achieve various target pressures across a wafer bonded with a uniform back-filled buffer gas pressure.Comment: 5 pages, 4 figure

Evolving the theory and praxis of knowledge translation through social interaction: a social phenomenological study

Background: As an inherently human process fraught with subjectivity, dynamic interaction, and change, social interaction knowledge translation (KT) invites implementation scientists to explore what might be learned from adopting the academic tradition of social constructivism and an interpretive research approach. This paper presents phenomenological investigation of the second cycle of a participatory action KT intervention in the home care sector to answer the question: What is the nature of the process of implementing KT through social interaction? Methods: Social phenomenology was selected to capture how the social processes of the KT intervention were experienced, with the aim of representing these as typical socially-constituted patterns. Participants (n = 203), including service providers, case managers, administrators, and researchers organized into nine geographically-determined multi-disciplinary action groups, purposefully selected and audiotaped three meetings per group to capture their enactment of the KT process at early, middle, and end-of-cycle timeframes. Data, comprised of 36 hours of transcribed audiotapes augmented by researchers\u27 field notes, were analyzed using social phenomenology strategies and authenticated through member checking and peer review. Results: Four patterns of social interaction representing organization, team, and individual interests were identified: overcoming barriers and optimizing facilitators; integrating \u27science push\u27 and \u27demand pull\u27 approaches within the social interaction process; synthesizing the research evidence with tacit professional craft and experiential knowledge; and integrating knowledge creation, transfer, and uptake throughout everyday work. Achieved through relational transformative leadership constituted simultaneously by both structure and agency, in keeping with social phenomenology analysis approaches, these four patterns are represented holistically in a typical construction, specifically, a participatory action KT (PAKT) model. Conclusion: Study findings suggest the relevance of principles and foci from the field of process evaluation related to intervention implementation, further illuminating KT as a structuration process facilitated by evolving transformative leadership in an active and integrated context. The model provides guidance for proactively constructing a \u27fit\u27 between content, context, and facilitation in the translation of evidence informing professional craft knowledge

Spectroscopic Observations of Convective Patterns in the Atmospheres of Metal-Poor Stars

Convective line asymmetries in the optical spectrum of two metal-poor stars, Gmb1830 and HD140283, are compared to those observed for solar metallicity stars. The line bisectors of the most metal-poor star, the subgiant HD140283, show a significantly larger velocity span that the expectations for a solar-metallicity star of the same spectral type and luminosity class. The enhanced line asymmetries are interpreted as the signature of the lower metal content, and therefore opacity, in the convective photospheric patterns. These findings point out the importance of three-dimensional convective velocity fields in the interpretation of the observed line asymmetries in metal-poor stars, and in particular, urge for caution when deriving isotopic ratios from observed line shapes and shifts using one-dimensional model atmospheres. The mean line bisector of the photospheric atomic lines is compared with those measured for the strong Mg I b1 and b2 features. The upper part of the bisectors are similar, and assuming they overlap, the bottom end of the stronger lines, which are formed higher in the atmosphere, goes much further to the red. This is in agreement with the expected decreasing of the convective blue-shifts in upper atmospheric layers, and compatible with the high velocity redshifts observed in the chromosphere, transition region, and corona of late-type stars.Comment: 27 pages, LaTeX; 10 Figures (14 PostScript files); to be published in The Astrophysical Journa