Microneedle array sensors based on carbon nanoparticle composites: interfacial chemistry and electroanalytical properties

Conductive microneedle patches consisting of carbon nanoparticles embedded in a polystyrene matrix have been prepared using micro-moulding techniques. The interfacial properties of the structures before and after electrochemical etching have been characterised using X-ray photoelectron spectroscopy and contact angle. Anodisation of the needles leads to a significant increase in oxygen functionality and is shown to dramatically improve the electroanalytical capabilities of the microneedle array. The detection of uric acid in horse blood was used as a model system through which to assess the performance of the system. The composite approach is shown to lead to viable carbon-based sensors and can offer a rapid prototype option for the development of tailored microneedle systems

The VO: A Powerful Tool for Global Astronomy

Since its inception in the early 2000's, the Virtual Observatory (VO), developed as a collaboration of many national and international projects, has become a major factor in the discovery and dissemination of astronomical information worldwide. The International Virtual Observatory Alliance (IVOA) has been coordinating all these efforts worldwide to ensure a common VO framework that enables transparent access to and interoperability of astronomy resources (data and software) around the world. The VO is not a magic solution to all astronomy data management challenges but it does bring useful solutions in many areas borne out by the fact that VO interfaces are broadly found in astronomy's major data centres and projects worldwide. Astronomy data centres have been building VO services on top of their existing data services to increase interoperability with other VO-compliant data resources to take advantage of the continuous and increasing development of VO applications. VO applications have made multi-instrument and multi-wavelength science, a difficult and fruitful part of astronomy, somewhat easier. More recently, several major new astronomy projects have been directly adopting VO standards to build their data management infrastructure, giving birth to ‘VO built-in' archives. Embracing the VO framework from the beginning brings the double gain of not needing to reinvent the wheel and ensuring from the start interoperability with other astronomy VO resources. Some of the IVOA standards are also starting to be used by neighbour disciplines like planetary sciences. There is still quite a lot to be done on the VO, in particular tackling the upcoming big data challenge and how to find interoperable solutions to the new data analysis paradigm of bringing and running the software close to the data. We report on the current status and also desire to encourage others to adopt VO technology and engage them in the effort of developing the VO. Thus, we wish to ensure that the VO standards fit new astronomy projects requirements and needs

inter and intra tumoral heterogeneity in dna damage evaluated by comet assay in early breast cancer patients

Abstract There are no clinical tools to functionally assess degree of DNA damage in breast cancer. The comet assay is an accepted research tool for assessing DNA damage, however, most cancer studies have assessed lymphocytes as surrogate cells. The aim of this pilot study was to use the comet assay in early breast cancer directly in tumor tissue to compare DNA damage between and within traditionally defined subgroups, and to explore intra-tumoral heterogeneity. Scrapings of tumor and healthy breast tissue were obtained at primary surgery from 104 women. Comet assay was applied to quantitatively assess DNA damage, revealing substantial inter- and intra-subgroup variation. Marked intra-tumoral heterogeneity was evident across all subgroups. The degree of DNA damage for an individual could not be predicted by breast cancer subgroup. Comet assay warrants further study as a potential clinical tool for identification of tumoral DNA damage and ultimately, individualised use of DNA damaging therapy

Runx1 deficiency protects against adverse cardiac remodeling following myocardial infarction

Background: Myocardial infarction (MI) is a leading cause of heart failure and death worldwide. Preservation of contractile function and protection against adverse changes in ventricular architecture (cardiac remodeling) are key factors to limiting progression of this condition to heart failure. Consequently, new therapeutic targets are urgently required to achieve this aim. Expression of the Runx1 transcription factor is increased in adult cardiomyocytes after MI; however, the functional role of Runx1 in the heart is unknown. Methods: To address this question, we have generated a novel tamoxifen-inducible cardiomyocyte-specific Runx1-deficient mouse. Mice were subjected to MI by means of coronary artery ligation. Cardiac remodeling and contractile function were assessed extensively at the whole-heart, cardiomyocyte, and molecular levels. Results: Runx1-deficient mice were protected against adverse cardiac remodeling after MI, maintaining ventricular wall thickness and contractile function. Furthermore, these mice lacked eccentric hypertrophy, and their cardiomyocytes exhibited markedly improved calcium handling. At the mechanistic level, these effects were achieved through increased phosphorylation of phospholamban by protein kinase A and relief of sarco/endoplasmic reticulum Ca2+-ATPase inhibition. Enhanced sarco/endoplasmic reticulum Ca2+-ATPase activity in Runx1-deficient mice increased sarcoplasmic reticulum calcium content and sarcoplasmic reticulum–mediated calcium release, preserving cardiomyocyte contraction after MI. Conclusions: Our data identified Runx1 as a novel therapeutic target with translational potential to counteract the effects of adverse cardiac remodeling, thereby improving survival and quality of life among patients with MI

The VO: A Powerful Tool for Global Astronomy

Since its inception in the early 2000's, the Virtual Observatory (VO), developed as a collaboration of many national and international projects, has become a major factor in the discovery and dissemination of astronomical information worldwide. The International Virtual Observatory Alliance (IVOA) has been coordinating all these efforts worldwide to ensure a common VO framework that enables transparent access to and interoperability of astronomy resources (data and software) around the world. The VO is not a magic solution to all astronomy data management challenges but it does bring useful solutions in many areas borne out by the fact that VO interfaces are broadly found in astronomy's major data centres and projects worldwide. Astronomy data centres have been building VO services on top of their existing data services to increase interoperability with other VO-compliant data resources to take advantage of the continuous and increasing development of VO applications. VO applications have made multi-instrument and multi-wavelength science, a difficult and fruitful part of astronomy, somewhat easier. More recently, several major new astronomy projects have been directly adopting VO standards to build their data management infrastructure, giving birth to ‘VO built-in' archives. Embracing the VO framework from the beginning brings the double gain of not needing to reinvent the wheel and ensuring from the start interoperability with other astronomy VO resources. Some of the IVOA standards are also starting to be used by neighbour disciplines like planetary sciences. There is still quite a lot to be done on the VO, in particular tackling the upcoming big data challenge and how to find interoperable solutions to the new data analysis paradigm of bringing and running the software close to the data. We report on the current status and also desire to encourage others to adopt VO technology and engage them in the effort of developing the VO. Thus, we wish to ensure that the VO standards fit new astronomy projects requirements and needs

Development of quality indicators for monitoring outcomes of frail elderly hospitalised in acute care health settings: Study Protocol

Background: Frail older people admitted to acute care hospitals are at risk of a range of adverse outcomes, including geriatric syndromes, although targeted care strategies can improve health outcomes for these patients. It is therefore important to assess inter-hospital variation in performance in order to plan and resource improvement programs. Clinical quality outcome indicators provide a mechanism for identifying variation in performance over time and between hospitals, however to date there has been no routine use of such indicators in acute care settings. A barrier to using quality indicators is lack of access to routinely collected clinical data. The interRAI Acute Care (AC) assessment system supports comprehensive geriatric assessment of older people within routine daily practice in hospital and includes process and outcome data pertaining to geriatric syndromes. This paper reports the study protocol for the development of aged care quality indicators for acute care hospitals. Methods/Design. The study will be conducted in three phases:. 1. Development of a preliminary inclusive set of quality indicators set based on a literature review and expert panel consultation,. 2. A prospective field study including recruitment of 480 patients aged 70 years or older across 9 Australian hospitals. Each patient will be assessed on admission and discharge using the interRAI AC, and will undergo daily monitoring to observe outcomes. Medical records will be independently audited, and. 3. Analysis and compilation of a definitive quality indicator set, including two anonymous voting rounds for quality indicator inclusion by the expert panel. Discussion. The approach to quality indicators proposed in this protocol has four distinct advantages over previous efforts: the quality indicators focus on outcomes; they can be collected as part of a routinely applied clinical information and decision support system; the clinical data will be robust and will contribute to better understanding variations in hospital care of older patients; The quality indicators will have international relevance as they will be built on the interRAI assessment instrument, an internationally recognised clinical system