Current understanding of hypospadias: relevance of animal models

Hypospadias is a congenital abnormality of the penile urethra with an incidence of approximately 1:200-1:300 male births, which has doubled over the past three decades. The aetiology of the overwhelming majority of hypospadias remains unknown but appears to be a combination of genetic susceptibility and prenatal exposure to endocrine disruptors. Reliable animal models of hypospadias are required for better understanding of the mechanisms of normal penile urethral formation and hence hypospadias. Mice and/or rats are generally used for experimental modelling of hypospadias, however these do not fully reflect the human condition. To use these models successfully, researchers must understand the similarities and differences between mouse, rat and human penile anatomy as well as the normal morphogenetic mechanisms of penile development in these species. Despite some important differences, numerous features of animal and human hypospadias are shared: the prevalence of distal penile malformations; disruption of the urethral meatus; disruption of urethra-associated erectile bodies; and a common mechanism of impaired epithelial fusion events. Rat and mouse models of hypospadias are crucial to our understanding of hypospadias to ultimately reduce its incidence through better preventive strategies

The Hurricane-Flood-Landslide Continuum

In August 2004, representatives from NOAA, NASA, the USGS, and other government agencies convened in San Juan, Puerto Rim for a workshop to discuss a proposed research project called the Hurricane-Flood-Landslide Continuum (HFLC). The essence of the HFLC is to develop and integrate tools across disciplines to enable the issuance of regional guidance products for floods and landslides associated with major tropical rain systems, with sufficient lead time that local emergency managers can protect vulnerable populations and infrastructure. All three lead agencies are independently developing precipitation-flood-debris flow forecasting technologies, and all have a history of work on natural hazards both domestically and overseas. NOM has the capability to provide tracking and prediction of storm rainfall, trajectory and landfall and is developing flood probability and magnTtude capabilities. The USGS has the capability to evaluate the ambient stability of natural and man-made landforms, to assess landslide susceptibilities for those landforms, and to establish probabilities for initiation of landslides and debris flows. Additionally, the USGS has well-developed operational capacity for real-time monitoring and reporting of streamflow across distributed networks of automated gaging stations (http://water.usgs.gov/waterwatch/). NASA has the capability to provide sophisticated algorithms for satellite remote sensing of precipitation, land use, and in the future, soil moisture. The Workshop sought to initiate discussion among three agencies regarding their specific and highly complimentary capabilities. The fundamental goal of the Workshop was to establish a framework that will leverage the strengths of each agency. Once a prototype system is developed for example, in relatively data-rich Puerto Rim, it could be adapted for use in data-poor, low-infrastructure regions such as the Dominican Republic or Haiti. This paper provides an overview of the Workshop s goals, presentations and recommendations with respect to the development of the HFLC

EC81-219, 1981 Nebraska Swine Report

This 1981 Nebraska Swine Report was prepared by the staff in Animal Science and cooperating departments for use in the Extension and Teaching programs at the University of Nebraska-Lincoln. Authors from the following areas contributed to this publication: Swine Nutrition, swine diseases, pathology, economics, engineering, swine breeding, meats, agronomy, and diagnostic laboratory. It covers the following areas: breeding, disease control, feeding, nutrition, economics, housing and meats

Development of the external genitalia: Perspectives from the spotted hyena (Crocuta crocuta)

This review/research paper summarizes data on development of the external genitalia of the spotted hyena, a fascinating mammal noted for extreme masculinization of the female external genitalia. The female spotted hyena is the only extant mammal that mates and gives birth through a pendulous penis-like clitoris. Our studies indicate that early formation of the phallus in both males and females is independent of androgens; indeed the phallus forms before the fetal testes or ovaries are capable of synthesizing androgens. Likewise, pre- and postnatal growth in length of the penis and clitoris is minimally affected by “androgen status”. Nonetheless, several internal morphologies, as well as external surface features of the phallus, are androgen-dependent and thus account for dimorphism between the penis and clitoris. Finally, estrogens play a critical role in penile and clitoral development, specifying the position of the urethral orifice, determining elasticity of the urethral meatus, and facilitating epithelial-epithelial fusion events required for proper formation of the distal urethra/urogenital sinus and prepuce. Accordingly, prenatal inhibition of estrogen synthesis via administration of letrozole (an aromatase inhibitor) leads to malformations of the glans as well as the prepuce (hypospadias). The effects of prenatal androgens, anti-androgens and impaired estrogen synthesis correlated with the tissue expression of androgen and estrogen receptors