Estimation of methane generation based on anaerobic digestion and mass balance at Kiteezi Landfill, Kampala, Uganda

Kiteezi landfill site is the main solid waste dumping site in Kampala City (Uganda). In this study, the generation of methane from waste at Kiteezi landfill was measured using laboratory-scale anaerobic digestion experiment and estimated using the Mass balance model. The samples were collected in the wet and dry seasons, with five replicates for each season which were processed for further experiments focused on moisture content analysis and anaerobic digestion. The moisture content analysis results showed a significant change (P < 0.05) between wet season and dry season. Also, the anaerobic digestion revealed that moisture content was a determining factor in gas generation. The average monthly methane production estimate from the mass balance model was 1.63 Gg methane/month and was comparable (within 14%) to the amount estimated by laboratory-scale anaerobic digestion experiment (1.43 Gg methane/month). It is a worthwhile undertaking to further investigate the potential of commercially producing methane from Kiteezi landfill as an alternative source of green and clean energy for urban masses.Keywords: Solid waste management, methane generation, anaerobic digestion and mass balance model

An Experimental Study to Determine the Antibacterial Activity of Selected Petroleum Jellies against Selected Bacteria that cause Skin Infections.

Background: The skin is the largest organ of the body and forms its first line of defense against pathogens. When the integrity of this natural protective barrier is compromised, it’s an opportune moment for pathogenic microorganisms such as Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus pyogenes among others to invade the body causing skin infections such as folliculitis, acne, impetigo among others. Objectives: This study aimed at determining the phytochemical profile of the selected petroleum jellies, determining the antibacterial activity of different petroleum jellies on selected bacteria causing skin infections, comparing the antibacterial activity of the jellies to that of the commonly used drugs against skin infections, and determining the minimum inhibitory concentration (MIC) of the jellies exhibiting antibacterial activity. Methods: The antibacterial activity of the jellies was determined by the agar well diffusion method (AWD) and the minimum inhibitory concentration (MIC) was determined by the broth dilution method. Results: Only herbal jellies exhibited antibacterial activity against at most two of the three bacterial species studied. The MIC values for the herbal jellies ranged from 47 mg/ml to 188 mg/ml. Conclusion: The non-herbal petroleum jellies did not show antibacterial activity while that of herbal jellies was minimal with very low potency and thus should not be relied on for wound healing or curing skin infections. Recommendations: The antibacterial activity of jellies should be tracked diligently to detect and address antimicrobial resistance as it arises to ensure that they remain efficacious

Agony resulting from cultural practices of canine bud extraction among children under five years in selected slums of Makindye : a cross sectional study

Background: Canine Bud Extraction (CBE) is a process of removing or gouging children's healthy canine tooth buds embedded underneath the gum using traditional unsterilized tools. The practice of CBE commonly known as false teeth removal continues to be an adopted cultural intervention of choice, in the prevention of morbidity and mortality from common childhood illnesses. However, it is a practice against the rights of the children with serious consequences. While CBE is associated with the perceived myth of curative gains, the agony emanating from the cultural practice exposes children to ill-health conditions such as dehydration, malnutrition, blood-borne diseases like HIV/AIDs, septicemia, fever and death. This research sought to understand the factors underpinning the practice of CBE among urban slum dwellers. Method: A cross-sectional study was conducted from five randomly selected slums in Makindye division; 298 household heads or guardians with children below 5 years, who had ever suffered from false teeth were interviewed. The variables measured included guardians' socio-demographic profiles, determinants of CBE, common childhood illnesses assumed to be treated with CBE and the reported side-effects associated with the practice. Results: Of the 298 respondents with children who had ever suffered from "false teeth" interviewed, 56.7% had two or more children below 5years and 31.9% were from the central region. The proportion of households practicing CBE was 90.3%; 69.8% of the caretakers mentioned that it was done by traditional healers and for 12.1% by trained health workers (dentists). Number of children (OR=2.8, 95% CI: 1.1-7.2) and the belief that CBE is bad (OR=0.1, 95% CI: <0.001, p<0.001) had a statistically significant association with CBE. Additionally, number of children (χ2=4.9, p=0.027) and 2 sets of beliefs (CBE treats diarrhea (χ2=12.8, p=0.0017) and CBE treats fever (χ2=15.1, p=0.0005) were independent predictors of CBE practice. A total of 55.7% respondents knew that there were side effects to CBE and 31% mentioned death as one of them. Conclusion: The high proportion of households practicing CBE from this study ought to awaken the perception that the practice is ancient. CBE in this community as the study suggests was strongly driven by myths. The strong belief that CBE is bad provides an opportunity for concerted effort by primary health care providers, policy makers and the community to demystify the myths associated with false teeth and the gains of CBE

A Digital One Health framework to integrate data for public health decision-making

The current implementation of One Health (OH) primarily focuses on multi-sectoral collaboration but often overlooks opportunities to integrate contextual and pathogen-related data into a unified data resource. This lack of integration hampers effective, data-driven decision-making in OH activities. In this perspective, we examine the existing strategies for data sharing and identify gaps and barriers to integration. To overcome these challenges, we propose the Digital OH (DOH) framework for data integration, which consolidates data-sharing principles within five pillars for the OH community of practice: (a) Harmonization of standards to establish trust, (b) Automation of data capture to enhance quality and efficiency, (c) Integration of data at point of capture to limit bureaucracy, (d) Onboard data analysis to articulate utility, and (e) Archiving and governance to safeguard the OH data resource. We discuss an upcoming pilot program as a use case focusing on antimicrobial resistance surveillance to illustrate the application of this framework. Our ambition is to leverage technology to create data as a shared resource using DOH not only to overcome current structural barriers but also to address prevailing ethical and legal concerns. By doing so, we can enhance the efficiency and effectiveness of decision-making processes in the OH community of practice, at a national, regional, and international level

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ПРОФЕССИОНАЛЬНАЯ ЗНАЧИМОСТЬ ЛИЧНОСТИПРОФЕССИИ ВЫБО

Antimicrobial susceptibility surveillance and antimicrobial resistance in Neisseria gonorrhoeae in Africa from 2001 to 2020: A mini-review

Antimicrobial resistance (AMR) in Neisseria gonorrhoeae (NG), compromising gonorrhea treatment, is a global public health concern. Improved, quality-assured NG AMR monitoring at the global level is essential. This mini-review examined NG AMR susceptibility surveillance and AMR data from the African continent from 2001 to 2020. Eligible peer-reviewed publications (n = 30) containing NG AMR data for antimicrobials currently recommended for gonorrhea treatment were included. Overall, very limited NG surveillance and AMR data was available. Furthermore, the NG AMR surveillance studies varied greatly regarding surveillance protocols (e.g., populations and samples tested, sample size, antimicrobials examined), methodologies (e.g., antimicrobial susceptibility testing method [agar dilution, minimum inhibitory concentration (MIC) gradient strip test, disc diffusion test] and interpretative criteria), and quality assurance (internal quality controls, external quality assessments [EQA], and verification of AMR detected). Moreover, most studies examined a suboptimal number of NG isolates, i.e., less than the WHO Global Gonococcal Antimicrobial Surveillance Program (GASP) and WHO Enhanced GASP (EGASP) recommendations of ≥100 isolates per setting and year. The notable inter-study variability and frequently small sample sizes make appropriate inter-study and inter-country comparisons of AMR data difficult. In conclusion, it is imperative to establish an enhanced, standardized and quality-assured NG AMR surveillance, ideally including patient metadata and genome sequencing as in WHO EGASP, in Africa, the region with the highest gonorrhea incidence globally. This will enable the monitoring of AMR trends, detection of emerging AMR, and timely refinements of national and international gonorrhea treatment guidelines. To achieve this aim, national and international leadership, political and financial commitments are imperative

Career development for infection and immunity research in Uganda: a decade of experience from the Makerere University – Uganda Virus Research Institute research and training programme

Background: The Makerere University/Uganda Virus Research Institute (UVRI) Centre of Excellence for Infection & Immunity Research and Training (MUII) is a collaborative programme supporting excellence in Infection and Immunity (I&I) research in Uganda. Set up in 2008, MUII aims to produce internationally competitive Ugandan and East African I&I research leaders, and develop human and infrastructural resources to support research and training excellence. We undertook an internal evaluation of MUII’s achievements, challenges and lessons learned between 08-2008 and 12-2019, to inform programmes seeking to build Africa’s health research expertise. Methods: : Quantitative data were abstracted from programme annual reports. Qualitative data were obtained in 03-04/2019: a cross-sectional evaluation was undertaken among a purposefully selected representative sample of 27 trainees and two programme staff. Qualitative data was analysed according to pre-determined themes of achievements, challenges, lessons learned and recommendations for improvement. Results: : By 12-2019, MUII had supported 68 fellowships at master’s-level and above (50% female: 23 Masters, 27 PhD, 15 post-doctoral, three group-leaders) and over 1,000 internships. Fellows reported career advancement, mentorship by experts, and improved research skills and outputs. Fellows have published over 300 papers, secured grants worth over £20m, established over 40 international collaborations, and taken on research and academic leadership positions in the country. Key lessons were: i) Efficient administration provides a conducive environment for high quality research; ii) Institutions need supportive policies for procurement, including provisions for purchases of specific biological research reagents from international manufacturers; iii) Strong international and multi-disciplinary collaboration provides a critical mass of expertise to mentor researchers in development; and iv) Mentorship catalyses young scientists to progress from graduate trainees to productive academic researchers, relevant to society’s most pressing health challenges. Conclusions: : Sustainable academic productivity can be achieved through efficient operational support, global collaboration and mentorship to provide solutions to Africa’s health challenges

Laboratory Diagnosis of Candidiasis

The burden of Candidiasis continues to increase and so does the Candida species. Although Candida species are closely similar phenotypically, they differ from each other in terms of epidemiology, genetic characteristics, antifungal susceptibility and virulence profile. Therefore, reliable and accurate laboratory methods for identification of Candida species can determine the Candidiasis burden and enable the administration of the most appropriate antifungal drug therapy to reduce fungal mortality rates. Conventional and biochemical methods are often used in identification of Candida species. However, these techniques are specific and sensitive enough in detecting the non albicans candida (NAC) species. Molecular techniques have improved the laboratory diagnosis and management of Candidiasis due to improved sensitivity and specificity threshold. This chapter provides an overview of different laboratory methods for diagnosis of Candidiasis

Serological and molecular investigation for brucellosis in swine in selected districts of Uganda

Brucellosis is a notifiable zoonotic disease affecting livestock, humans and wildlife in Uganda. Human brucellosis cases are frequently reported and the increasing incidence is suggestive of increasing disease in the livestock population. Pigs are among the livestock species that can be infected with human pathogenic Brucella suis biovars 1 and 3 and can be a significant source of brucellosis for humans. Uganda has a rapidly growing pig population and the pork consumption per capita is the highest in East Africa. The objective of this work was to determine the seroprevalence of brucellosis in Ugandan pigs

Microbial characteristics of dental caries in HIV positive individuals

BACKGROUND: Dental caries is a multifactorial disease that affects many people. Even though microorganisms play a crucial role in causing dental caries, diagnosis is routinely macroscopic. In order to improve early detection especially in HIV patients who are disproportionately affected, there is need to reconcile the macroscopic and microscopic characteristics of dental caries. Therefore, the aim of this study was to characterize the oral microbiota profile along the decayed, missing, filled teeth (DMFT) index using amplicon sequencing data. METHODS: Amplicon sequencing of the V6-V8 region of the 16S rRNA gene was done on DNA recovered from whole unstimulated saliva of 59 HIV positive and 29 HIV negative individuals. The microbial structure, composition and co-occurrence networks were characterized using QIIME-2, Phyloseq, Microbiome-1.9.2 and Metacoder in R. RESULTS: We characterized the oral microbiota into 2,093 operational taxonomic units (OTUs), 21 phyla and 239 genera from 2.6 million high quality sequence reads. While oral microbiota did not cluster participants into distinct groups that track with the DMFT index, we observed the following: (a) The proportion of accessory microbiota was highest in the high DMFT category while the core size (∼50% of richness) remained relatively stable across all categories. (b) The abundance of core genera such as Stomatobaculum, Peptostreptococcus and Campylobacter was high at onset of dental caries, (c) A general difference in oral microbial biomass. (d) The onset of dental caries (low DMFT) was associated with significantly lower oral microbial entropy. CONCLUSIONS: Although oral microbial shifts along the DMFT index were not distinct, we demonstrated the potential utility of microbiota dynamics to characterize oral disease. Therefore, we propose a microbial framework using the DMFT index to better understand dental caries among HIV positive people in resource limited settings