Students’ Experiences in Interdisciplinary Problem-based Learning: A Discourse Analysis of Group Interaction

Interdisciplinary problem-based learning (PBL) aims to provide students with opportunities to develop the necessary skills to work with different health professionals in a collaborative manner. This discourse study examined the processes of collective knowledge construction in Japanese students in the tutorials. Analyses of video-recorded data elicited from three multidisciplinary cohorts and their learning portfolios provided insights into their participation and introspection during the discussions. The results indicate there were two patterns of knowledge construction: (a) co-constructions between students from different disciplines and (b) elaborations between students from the same discipline. Their learning processes were mediated by their cultural assumptions, professional identities, understanding of other professionals, and perceptions of collaborative learning. The finding suggests that interdisciplinary PBL has the potential to enhance students’ collaborative learning skills, and students’ participation is situated within a cultural context

Basic fibroblastic growth factor affects the osteogenic differentiation of dental pulp stem cells in a treatment‐dependent manner

AimTo determine how basic fibroblastic growth factor (bFGF) affected the osteogenic differentiation of human dental pulp stem cells (DPSCs) in vitro and in vivo.MethodologyBasic fibroblastic growth factor stimulation of DPSCs was divided into a pre‐treatment period and an osteogenic differentiation period. Alizarin red quantification experiments and alkaline phosphatase activity quantification assay were performed to examine the osteogenic differentiation of DPSCs after different bFGF stimulation. Quantification reverse transcription polymerase chain reaction was used to analyze the osteogenic gene expression of DPSCs after different bFGF stimulation. In addition, DPSCs that received the 1 and 2 weeks bFGF pre‐treatments as in the in vitro experiments were mineralized for 1 week and seeded into hydroxyapatite/tricalcium phosphate (HA/TCP) pills and subcutaneously transplanted into naked mice for 2 or 3 months. The transplants were removed, sliced and stained using Modified Ponceau Trichrome Stain to observe the formation of mineralized tissue.ResultsBasic fibroblastic growth factor stimulation in the osteogenic differentiation period decreased the in vitro osteogenic differentiation ability of DPSCs. One week pre‐treatment with bFGF increased the in vitro osteogenic differentiation ability of DPSCs, whereas 2 weeks pre‐treatment with bFGF decreased the in vitro osteogenic differentiation ability of DPSCs. The pre‐treatment period was vital for the osteogenic differentiation of DPSCs in vitro. The in vivo results were similar to the in vitro results.ConclusionsBasic fibroblastic growth factor affected the osteogenic differentiation of DPSCs in a treatment‐dependent manner both in vitro and in vivo.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/111933/1/iej12368.pd

Formation of Zwitterionic Fullerodendron Using a New DBN-Focal Dendron

A new poly(amidoamine) dendron having 1,5-diazabicyclo[4.3.0]non-5-ene (DBN) at the focal point was synthesized. Interestingly, formation of zwitterionic fullerodendrons (λmax = 930 nm for C60 and 795 nm for C70) were observed by Vis-NIR spectroscopy upon the reaction of C60 or C70 with the DBN-focal dendron. In particular, the C70 anion was effectively stabilized by the site isolation effect of the dendritic wedge. The half-life of fullerodendron 12b having C70 anion at the focal point reaches 7,345 min, which is 20 times longer than that of complex between C60 and pristine DBN. Furthermore, in order to confirm the structure of the zwitterionic complex, fullerodendron 12a was reprecipitated from benzonitrile/1,2,4-trimethylbenzene, and was observed using IR spectroscopy and APPI-MS

Proteoglycan 4: A dynamic regulator of skeletogenesis and parathyroid hormone skeletal anabolism

Proteoglycan 4 ( Prg4 ), known for its lubricating and protective actions in joints, is a strong candidate regulator of skeletal homeostasis and parathyroid hormone (PTH) anabolism. Prg4 is a PTH‐responsive gene in bone and liver. Prg4 null mutant mice were used to investigate the impact of proteoglycan 4 on skeletal development, remodeling, and PTH anabolic actions. Young Prg4 mutant and wild‐type mice were administered intermittent PTH(1–34) or vehicle daily from 4 to 21 days. Young Prg4 mutant mice had decreased growth plate hypertrophic zones, trabecular bone, and serum bone formation markers versus wild‐type mice, but responded with a similar anabolic response to PTH. Adult Prg4 mutant and wild‐type mice were administered intermittent PTH(1–34) or vehicle daily from 16 to 22 weeks. Adult Prg4 mutant mice had decreased trabecular and cortical bone, and blunted PTH‐mediated increases in bone mass. Joint range of motion and animal mobility were lower in adult Prg4 mutant versus wild‐type mice. Adult Prg4 mutant mice had decreased marrow and liver fibroblast growth factor 2 (FGF‐2) mRNA and reduced serum FGF‐2, which were normalized by PTH. A single dose of PTH decreased the PTH/PTHrP receptor (PPR), and increased Prg4 and FGF‐2 to a similar extent in liver and bone. Proteoglycan 4 supports endochondral bone formation and the attainment of peak trabecular bone mass, and appears to support skeletal homeostasis indirectly by protecting joint function. Bone‐ and liver‐derived FGF‐2 likely regulate proteoglycan 4 actions supporting trabeculae formation. Blunted PTH anabolic responses in adult Prg4 mutant mice are associated with altered biomechanical impact secondary to joint failure. © 2012 American Society for Bone and Mineral ResearchPeer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/89450/1/508_ftp.pd

Punta Querandí: disputas por el espacio entre las urbanizaciones cerradas y los movimientos sociales

El presente informe es el resultado del trabajo llevado a cabo por los alumnos del sexto año de la Escuela Secundaria del Colegio San Simón, de la ciudad de La Plata, en el marco de la asignatura de la Orientación en Ciencias Sociales denominada Proyectos de Investigación en Ciencias Sociales, durante el ciclo lectivo 2012. Se estudió el conflicto alrededor del paraje denominado Punta Querandí, ubicado entre los partidos de Tigre y Escobar, para abordar la problemática de las disputas por el espacio entre las urbanizaciones cerradas y los movimientos sociales. Se da cuenta de las sucesivas etapas de elaboración del diseño de investigación, la realización del trabajo de campo y las conclusiones alcanzadas al finalizar el año lectivo.Facultad de Humanidades y Ciencias de la Educació

Microscopical methods for the localization of Na + , K + -ATPase

Na + , K + -ATPase plays a central role in the ionic and osmotic homeostasis of cells and in the movements of electrolytes and water across epithelial boundaries. Microscopic localization of the enzyme is, therefore, of crucial importance in establishing the subcellular routes of electrolyte flow across structurally complex and functionally polarized epithelia. Recently developed approaches to the localization of Na + , K + -ATPase are reviewed. These methods rely on different properties of the enzyme and encompass cytochemical localization of the K + -dependent nitrophenylphosphatase component of the enzyme, autoradiographic localization of tritiated ouabain binding sites, and immunocytochemical localization of the holoenzyme and of its catalytic subunit. The rationales for each of these techniques are outlined as are the critieria that have been established to validate each method. The observed localization of Na + , K + -ATPase in various tissues is discussed, particularly as it relates to putative and hypothetical mechanisms that are currently thought to mediate reabsorptive and secretory electrolyte transport.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/42850/1/10735_2005_Article_BF01005056.pd

ラットジンニオケルウワバインカンジュセイ カリウムイソンセイパラニトロフェニルホスファターゼ カッセイノチョウビキョクザイ

京都大学0048新制・論文博士医学博士乙第4211号論医博第846号新制||医||278(附属図書館)6601UT51-57-E276(主査)教授 濱島 義博, 教授 吉田 修, 教授 小川 和朗学位規則第5条第2項該当Kyoto UniversityDA

マウスヒカソシキキュウノデンケンソシキカガクテキケンキュウ

京都大学0048新制・論文博士理学博士乙第2191号論理博第416号新制||理||170(附属図書館)3585UT51-48-A29(主査)教授 白上 謙一, 教授 岡田 節人, 教授 加藤 幹太学位規則第5条第2項該当Kyoto UniversityDA