Rupture de matériaux mous : De l’élasticité linéaire à l’endommagement aux échelles microscopiques

Our novel experimental approach consists in studying fracture mechanics of soft materials, mainly polymer and colloidal gels, which have microstructures with large typical length scales. This increase in the microscopic length scale will consequently increase the typical size of the process zone and make its observation easier with standard microscopy techniques (optical or confocal).To do so, we designed a novel experimental device to study crack propagation in such soft materials. This experiment enables us to grow a unique crack in a controlled way in a soft specimen and to look at the crack tip at high magnification for crack velocities between 1 µm/s and 1cm/s. Working on physical polymer gels, we analysed the crack shape and crack displacement fields (using Digital Image Correlation) at large and intermediate scales for various velocities. We realized there was a separation of scales between the scale at which LEFM applies, the scale at which elastic nonlinearities emerge and the scale at which dissipation occurs. This last scale could not be investigated with the polymer gel. Recent experiments on colloidal gels, which have a microscopic length scale bigger than the one of polymer gels, show that we are able to probe damage at the microstructural scale.Notre nouvelle approche expérimentale consiste à étudier la fissuration de matériaux mous, principalement des gels polymériques et colloidaux, qui ont des tailles microstructurales micrométriques. Cette augmentation de la taille microscopique va avoir pour conséquence d’augmenter la taille de la zone de process et va rendre son observation plus facile avec des moyens standard de microscopie (à transmission et confocale).Pour se faire, nous avons mis au point un nouveau dispositif expérimental pour étudier la propagation de fissures dans des matériaux mous. Cette expérience permet de faire croître une fissure de manière contrôlée dans un échantillon mou et d’inspecter la pointe de fissure à haute résolution pour des fissures se propageant entre 1 µm/s and 1cm/s. En travaillant avec des gels de polymère physiques, nous avons analyse la forme de fissure ainsi que les champs de déplacement proches pointe (en utilisant des techniques de corrélation d’image) à petites et grandes échelles et à différentes vitesses. Nous avons montré qu’il existait une séparation d’échelles spatiales entre les échelles où l’élasticité linéaire s’applique, les échelles auxquelles les non linéarités émergent et les échelles auxquelles la dissipation se produit. Cette dernière échelle n’a pas pu être investigué dans le cas de gels polymériques. De récentes expériences sur des gels colloïdaux, ayant une longueur micro-structurale plus grande que celle des gels polymers, montre que nous sommes capables de sonder en temps réel les échelles d’endommagement lors de la fissuration

Heterogeneity in tribologically transformed structure (TTS) of Ti-6Al-4V under fretting

Fretting wear is a surface degradation process caused by oscillatory motion and contact slipping. During gross slip, high local stresses and plastic deformation in the surface and subsurface can lead to the creation of a nanosized grained structure called Tribologically Transformed Structure (TTS). The current paper studies the formation of TTS in an alpha-beta Ti-6Al-4V alloy under fretting loading while changing the contact pressure and the number of fretting cycles.Cross-sections of wear scars are observed after polishing and chemical etching. Above a threshold pressure of 300 MPa, TTS appears early in the contact (before 1000 cycles) along with two other structures: a Third Body Layer (TBL) made of compacted debris and a General Deformed Layer (GDL) which is the plastic zone under the TTS. TTS first appears as islands and merges in the middle of the contact after enough cycles. Below 200 MPa, only TBL and GDL are formed. At 200 MPa, only small, localized TTS is found. All structures have the same chemical compositions as the initial bulk material except for the nitrided TBL. TTS has a very high hardness compared to the bulk. TTS was carefully extracted using a Focused Ion Beam (FIB) and its microstructure was observed with a Transmission Electron Microscope (TEM). It shows extreme grain refinement and is composed of two alternated zones. The first zone I is composed of $\alpha$ grains with a size of 20 to 50 nm with crystallographic texture. Zone II comprises nanosized equiaxed grains whose sizes range from 5 to 20 nm without texture. The results made it possible to establish a scenario of the appearance of the TTS according to the conditions of contact pressure and number of fretting cycles

The gut-lung axis in the CFTR modulator era

The advent of CFTR modulators represents a turning point in the history of cystic fibrosis (CF) management, changing profoundly the disease’s clinical course by improving mucosal hydration. Assessing changes in airway and digestive tract microbiomes is of great interest to better understand the mechanisms and to predict disease evolution. Bacterial and fungal dysbiosis have been well documented in patients with CF; yet the impact of CFTR modulators on microbial communities has only been partially deciphered to date. In this review, we aim to summarize the current state of knowledge regarding the impact of CFTR modulators on both pulmonary and digestive microbiomes. Our analysis also covers the inter-organ connections between lung and gut communities, in order to highlight the gut-lung axis involvement in CF pathophysiology and its evolution in the era of novel modulators therapies

Rupture de matériaux mous : De l’élasticité linéaire\u2028à l’endommagement aux échelles microscopiques

Notre nouvelle approche expérimentale consiste à étudier la fissuration de matériaux mous, principalement des gels polymériques et colloidaux, qui ont des tailles microstructurales micrométriques. Cette augmentation de la taille microscopique va avoir pour conséquence d’augmenter la taille de la zone de process et va rendre son observation plus facile avec des moyens standard de microscopie (à transmission et confocale).Pour se faire, nous avons mis au point un nouveau dispositif expérimental pour étudier la propagation de fissures dans des matériaux mous. Cette expérience permet de faire croître une fissure de manière contrôlée dans un échantillon mou et d’inspecter la pointe de fissure à haute résolution pour des fissures se propageant entre 1 µm/s and 1cm/s. En travaillant avec des gels de polymère physiques, nous avons analyse la forme de fissure ainsi que les champs de déplacement proches pointe (en utilisant des techniques de corrélation d’image) à petites et grandes échelles et à différentes vitesses. Nous avons montré qu’il existait une séparation d’échelles spatiales entre les échelles où l’élasticité linéaire s’applique, les échelles auxquelles les non linéarités émergent et les échelles auxquelles la dissipation se produit. Cette dernière échelle n’a pas pu être investigué dans le cas de gels polymériques. De récentes expériences sur des gels colloïdaux, ayant une longueur micro-structurale plus grande que celle des gels polymers, montre que nous sommes capables de sonder en temps réel les échelles d’endommagement lors de la fissuration.Our novel experimental approach consists in studying fracture mechanics of soft materials, mainly polymer and colloidal gels, which have microstructures with large typical length scales. This increase in the microscopic length scale will consequently increase the typical size of the process zone and make its observation easier with standard microscopy techniques (optical or confocal).To do so, we designed a novel experimental device to study crack propagation in such soft materials. This experiment enables us to grow a unique crack in a controlled way in a soft specimen and to look at the crack tip at high magnification for crack velocities between 1 µm/s and 1cm/s. Working on physical polymer gels, we analysed the crack shape and crack displacement fields (using Digital Image Correlation) at large and intermediate scales for various velocities. We realized there was a separation of scales between the scale at which LEFM applies, the scale at which elastic nonlinearities emerge and the scale at which dissipation occurs. This last scale could not be investigated with the polymer gel. Recent experiments on colloidal gels, which have a microscopic length scale bigger than the one of polymer gels, show that we are able to probe damage at the microstructural scale

J Antimicrob Chemother

Caspofungin is an echinocandin antifungal agent that inhibits synthesis of glucan required for the fungal cell wall. Resistance is mediated by mutation of Fks1 glucan synthase, among which S645P is the most common resistance-associated polymorphism. Rapamycin is a macrolide that inhibits the mechanistic target of rapamycin (mTOR) protein kinase activity. This study investigated the interaction between rapamycin and caspofungin in inhibiting the growth of WT Candida albicans and Fks1 S645P mutant clinical isolate, and WT Candida lusitaniae and genetically engineered isogenic strain with Fks1 S645P mutation at equivalent position. Interactions between caspofungin and rapamycin were evaluated using the microdilution chequerboard method in liquid medium. The results were analysed using the Loewe additivity model (FIC index, FICI) and the Bliss independence model (response surface, RS, analysis). Synergy between rapamycin and caspofungin was shown for C. albicans and C. lusitaniae strains by RS analysis of the chequerboard tests. Synergy was observed in strains susceptible and resistant to caspofungin. Weak subinhibitory concentrations of rapamycin were sufficient to restore caspofungin susceptibility. We report here, for the first time, synergy between caspofungin and rapamycin in Candida species. Synergy was shown for strains susceptible and resistant to caspofungin. This study highlights the possible implication of the TOR pathway in sensing antifungal-mediated cell wall stress and in modulating the cellular response to echinocandins in Candida yeasts

Industrial Symbiosis in Insect Production—A Sustainable Eco-Efficient and Circular Business Model

Insect meal (IM) is a source of high-quality protein for aquafeed while insect oil (IO) is a source of fatty acids used in monogastric feed with identical or better performance than premium fishmeal (FM) or vegetable oils (VOs) respectively. Although insects’ ability to feed on agricultural by-products and the entire valorization of insect products (IM, IO, frass) suggest insect production is sustainable, no studies have documented its environmental impact using industrial-scale production data. The present study is the first attributional life cycle assessment (A-LCA) based on data from an industrial-scale facility implementing an innovative symbiosis production model. This A-LCA was used to (i) assess the environmental performance of the symbiosis model vs. a no-symbiosis model and (ii) compare the environmental impacts of IM and IO production vs. their respective alternatives. The results revealed that the symbiosis model introduces a meaningful change in terms of environmental footprint by reducing CO2 emissions by 80% and fossil resources depletion by 83% compared to the no-symbiosis model. The higher sustainability of the IM and IO produced using the symbiosis model was also demonstrated, as CO2 emissions were reduced by at least 55% and 83% when compared to the best FM and VOs alternatives, respectively

Heterogeneity in tribologically transformed structure (TTS) of Ti–6Al–4V under fretting

International audienceFretting wear is a surface degradation process caused by oscillatory motion and contact slipping. During gross slip, high local stresses and plastic deformation in the surface and subsurface can lead to the creation of a nanosized grained structure called Tribologically Transformed Structure (TTS). The current paper studies the formation of TTS in an alpha-beta Ti-6Al-4V alloy under fretting loading while changing the contact pressure and the number of fretting cycles.Cross-sections of wear scars are observed after polishing and chemical etching. Above a threshold pressure of 300 MPa, TTS appears early in the contact (before 1000 cycles) along with two other structures: a Third Body Layer (TBL) made of compacted debris and a General Deformed Layer (GDL) which is the plastic zone under the TTS. TTS first appears as islands and merges in the middle of the contact after enough cycles. Below 200 MPa, only TBL and GDL are formed. At 200 MPa, only small, localized TTS is found. All structures have the same chemical compositions as the initial bulk material except for the nitrided TBL. TTS has a very high hardness compared to the bulk. TTS was carefully extracted using a Focused Ion Beam (FIB) and its microstructure was observed with a Transmission Electron Microscope (TEM). It shows extreme grain refinement and is composed of two alternated zones. The first zone I is composed of α grains with a size of 20 to 50 nm with crystallographic texture. Zone II comprises nanosized equiaxed grains whose sizes range from 5 to 20 nm without texture. The results made it possible to establish a scenario of the appearance of the TTS according to the conditions of contact pressure and number of fretting cycles

Crustacean cardioactive peptides Expression, localization, structure, and a possible involvement in regulation of egg-laying in the cuttlefish Sepia officinalis

International audienceThe cuttlefish (Sepia officinalis) is a cephalopod mollusk distributed on the western European coast, in the West African Ocean and in the Mediterranean Sea. On the Normandy coast (France), cuttlefish is a target species of professional fishermen, so its reproduction strategy is of particular interest in the context of stock management. Egg-laying, which is coastal, is controlled by several types of regulators among which neuropeptides. The cuttlefish neuropeptidome was recently identified by Zatylny-Gaudin et al. (2016). Among the 38 neuropeptide families identified, some were significantly overexpressed in egg-laying females as compared to mature males. This study is focused on crustacean cardioactive peptides (CCAPs), a highly expressed neuropeptide family strongly suspected of being involved in the control of egg-laying. We investigated the functional and structural characterization and tissue mapping of CCAPs, as well as the expression patterns of their receptors. CCAPs appeared to be involved in oocyte transport through the oviduct and in mechanical secretion of capsular products. Immunocytochemistry revealed that the neuropeptides were localized throughout the central nervous system (CNS) and in the nerve endings of the glands involved in egg-capsule synthesis and secretion, i.e. the oviduct gland and the main nidamental glands. The CCAP receptor was expressed in these glands and in the subesophageal mass of the CNS. Multiple sequence alignments revealed a high level of conservation of CCAP protein precursors in Sepia officinalis and Loligo pealei, two cephalopod decapods. Primary sequences of CCAPs from the two species were fully conserved, and cryptic peptides detected in the nerve endings were also partially conserved, suggesting biological activity that remains unknown for the time being

Structural and Functional Characterization of Orcokinin B-like Neuropeptides in the Cuttlefish (Sepia officinalis)

International audienceThe cuttlefish (Sepia officinalis) is a Cephalopod mollusk that lives in the English Channel and breeds in coastal spawning grounds in spring. A previous work showed that the control of egg-laying is monitored by different types of regulators, among which neuropeptides play a major role. They are involved in the integration of environmental cues, and participate in the transport of oocytes in the genital tract and in the secretion of capsular products. This study addresses a family of neuropeptides recently identified and suspected to be involved in the control of the reproduction processes. Detected by mass spectrometry and immunocytochemistry in the nerve endings of the accessory sex glands of the females and ovary, these neuropeptides are also identified in the hemolymph of egg-laying females demonstrating that they also have a hormone-like role. Released in the hemolymph by the sub-esophageal mass, a region that innervates the genital tract and the neurohemal area of the vena cava, in in vitro conditions these neuropeptides modulated oocyte transport and capsular secretion. Finally, in silico analyses indicated that these neuropeptides, initially called FLGamide, had extensive structural homology with orcokinin B, which motivated their name change