Thirty years of invasion: the distribution of the invasive crayfish Procambarus clarkii in Italy

The presence of the red swamp crayfish Procambarus clarkii in Italy is documented since 1989, but no comprehensive data are available on its spread through time at the national scale. New confirmed records for Procambarus clarkii are continuously arising in recent years across the country. By reviewing the scientific and grey literature, we obtained an up-to-date map of the species invasion in Italy. This information can help to monitor and understand the spread of this highly invasive crayfish and to implement more effective management measures.

14q13 distal microdeletion encompassing NKX2-1 and PAX9: Patient report and refinement of the associated phenotype

Chromosome 14q11-q22 deletion syndrome (OMIM 613457) is a rare genomic disorder whose associated phenotype is heterogeneous, depending on the size, and, mostly, on the deleted region. We report the clinical and molecular characterization of a female newborn, whose phenotype was characterized by poor growth, dysmorphic facial features, subclinical hypothyroidism, and mild reduction of CD3CD8 Lymphocytes with increased CD4/CD8 ratio. By array-CGH, we identified a 4.08 de novo interstitial deletion of the 14q13.2q21.1 region, which includes 16 OMIM genes.Our patient phenotype is compared with other published cases, for a better classification of the 14q11-q22 deletion syndrome. We demonstrated that the 14q13.2q21.1 deletion, which encompasses NKX2-1, but not FOXG1 gene and HPE8 region, identifies a well defined, more benign, microdeletion syndrome. This report confirms that an early identification with accurate characterization of the genomic disorders is of great relevance, enabling proper genetic counseling of the reproductive risk, as well as disease prognosis, and patient management. © 2016 Wiley Periodicals, Inc

Simplified Ray Tracing for the Millimeter Wave Channel: A Performance Evaluation

Millimeter-wave (mmWave) communication is one of the cornerstone innovations of fifth-generation (5G) wireless networks, thanks to the massive bandwidth available in these frequency bands. To correctly assess the performance of such systems, however, it is essential to have reliable channel models, based on a deep understanding of the propagation characteristics of the mmWave signal. In this respect, ray tracers can provide high accuracy, at the expense of a significant computational complexity, which limits the scalability of simulations. To address this issue, in this paper we present possible simplifications that can reduce the complexity of ray tracing in the mmWave environment, without significantly affecting the accuracy of the model. We evaluate the effect of such simplifications on link-level metrics, testing different configuration parameters and propagation scenarios.Comment: 6 pages, 6 figures, 1 table. This paper has been accepted for presentation at ITA 2020. (c) 2020 IEEE. Please cite it as: M. Lecci, P. Testolina, M. Giordani, M. Polese, T. Ropitault, C. Gentile, N. Varshney, A. Bodi, M. Zorzi, "Simplified Ray Tracing for the Millimeter Wave Channel: A Performance Evaluation," Information Theory and Applications Workshop (ITA), San Diego, US, 202

Estimating abundance and habitat suitability in a micro-endemic snake: the Walser viper

Recently described species suffer lack of information that hampers setting up appropriate conservation strategies. The situation is particularly complex with micro-endemic snakes, for which detection and monitoring are particularly challenging. The Walser viper Vipera walser is a recently described snake inhabiting a small area of the SW Italian alps. We combined information on species distribution with repeated monitoring to identify the areas most suitable for the species, and to obtain estimates of species abundance. Species distribution models were used to identify the topographical, climatic, and land-cover features related to the occurrence of vipers. Furthermore, repeated transects and N-mixture models were used to estimate abundance and to identify factors related to the variation of abundance. The available data suggested that the species has a disjunct range, with a Northern range of ~45 km2 , and a southern range of ~225 km2. Distribution models suggested that vipers are associated with areas with open egetation,altitude between 1300 and 2300 m, high precipitation, low forest cover, low slope, and southern aspect. N-mixture models confirmed very low detection probability of these vipers, and suggested that the species has a low abundance,with the highest abundance in south-facing plots. We provide the first quantitative information on habitats and abundance variation for Walser vipers. The broad confidence intervals of abundance estimates exemplify the complexity of providing range-wide measures of abundance for secretive species. Given the narrow range of these vipers, continuous monitoring is required to understand how they respond to ongoing environmental changes in mountainous areas

Wappfruit: a project for the optimisation of water use in agriculture

The WAPPFRUIT project is related to the optimisation of irrigation techniques in the Piemonte Region, Northwest Italy. The main goal is to control irrigation to understand if it is possible to reduce the volume of water used for irrigation and also save energy. The project involves several stakeholders, among which Politecnico and the University of Torino, Piemonte Region, Agrion Foundation for research in agriculture, and three farms (two apple orchards and one Actinidia orchard). The optimisation relies on soil matric potential measurements at several soil depths. The irrigation will be triggered using a particular algorithm which is based on a system of matric potential thresholds at the depths of 20 and 40 cm. These thresholds are based on soil texture, and vegetation species (including root depth). Each orchard is divided into two parts: an “experimental area” where the irrigation algorithm will be tested, and an area that will be irrigated as usual by farmers. Each orchard is equipped with four to six measurement nodes, with soil water content and soil matric potential profile having measures at 20, 40, and 60 cm of depth. The retention curves, as well as the spatial and temporal variability of soil water content and soil matric potential, can be inferred from measures, which reveal high volumes of water used for irrigation (frequently the soil was near saturation conditions). In addition, all the soils show, in the retention curves, a hysteresis due to wetting/drying cycles. The farmers continued to irrigate as usual in the two parts of the fields up to October 2022. Hence, to investigate the matric potential behavior and identify good estimates of thresholds, modeling approaches are important for the simulation of soil without irrigation, to understand when water stress conditions could occur. To this purpose, two models are used to simulate the water fluxes in the atmosphere and the soil (and, particularly, the matric potential). The two models adopted are the hydrological model Hydrus 1D and the land-surface model CLM5. Forcing the models with the precipitation summed to irrigation of the fields, Hydrus, in its 1D formulation did not yield reliable results, although more studies are needed to fully understand the causes for the misrepresentation. The CLM model yields instead more reliable outcomes. The CLM model is then used to simulate the behavior of the soil matric potential under the hypothesis of no irrigation. The results illustrate that the matric potential threshold for triggering irrigation could be around -50 kPa at 20 cm, whereas the threshold at 40 cm for the deactivation of irrigation could be around -40 kPa for the sites with apple orchards. The site with Actinidia could have the aforementioned thresholds equal to -40 kPa at 20 cm and -30 kPa at 40 cm

A framework for prioritising present and potentially invasive mammal species for a national list

The European Union (EU) has recently adopted a regulation on invasive alien species that foresees the possibility of developing lists of species of National Concern. We developed a prioritisation process for alien mammals already established in Italy, but not yet included in the EU list (n = 6 species) and a systematic horizon-scanning procedure to obtain ranked lists for those species that are already introduced worldwide or traded in Italy (n = 213). Experts were asked to score these species, by evaluating their likelihood of establishment and spread and the magnitude of their potential impacts on biodiversity, economy, human-health and society. The manageability of each species was also evaluated, both for the proritisation and the horizon-scanning processes. We produced five lists that ranked species according to their potential spread and impacts and their manageability. These will allow policy-makers to select outputs according to a balance between risk assessment and risk management, establishing priorities for alien species management at the national level

Combinatorial effects on gene expression at the Lbx1/Fgf8 locus resolve split-hand/foot malformation type 3

Split-Hand/Foot Malformation type 3 (SHFM3) is a congenital limb malformation associated with tandem duplications at the LBX1/FGF8 locus. Yet, the disease patho-mechanism remains unsolved. Here we investigate the functional consequences of SHFM3-associated rearrangements on chromatin conformation and gene expression in vivo in transgenic mice. We show that the Lbx1/Fgf8 locus consists of two separate, but interacting, regulatory domains. Re-engineering of a SHFM3-associated duplication and a newly reported inversion in mice results in restructuring of the chromatin architecture. This leads to ectopic activation of the Lbx1 and Btrc genes in the apical ectodermal ridge (AER) in an Fgf8-like pattern induced by AER-specific enhancers of Fgf8. We provide evidence that the SHFM3 phenotype is the result of a combinatorial effect on gene misexpression in the developing limb. Our results reveal insights into the molecular mechanism underlying SHFM3 and provide conceptual framework for how genomic rearrangements can cause gene misexpression and disease.This study was supported by grants from the Deutsche Forschungsgemeinschaft (MU 880/16-1, MU 880/20-1) to S.M. We thank the transgenic unit, sequencing core and animal facility of Max Planck Institute for Molecular Genetics for technical assistance, Ute Fischer for technical support and Norbert Brieske for help with whole mount in situ hybridizations and image processing

High habitat invasibility unveils the invasiveness potential of water frogs

peer reviewedDetermining the invasibility of habitats by alien species is crucial for understanding their spread potential, the habitats the most at risk and to implement adequate management actions. This is urgent for introduced taxa that show high invasion potential across broad geographical scales. We here assess these processes in invasive Pelophylax water frogs which are widespread colonizers across Western Europe and for which the invasibility of habitats remains to be quantified. Specifically, we used hierarchical occupancy models in a Bayesian framework to identify local-and landscape-scale features that can enhance occupancy of the most common invasive water frog, the marsh frog (P. ridibundus), in southern France. Water frogs were highly detectable and showed high occupancy across the invaded landscape. The invaders expressed a very broad habitat tolerance for both local-and landscape-scale variables while their invasion was facilitated by the occurrence of deep, permanent ponds with abundant aquatic vegetation and high sun exposure. Cross-validation showed a good transferability of models across space. The high invasibility of a wide range of habitats by Pelophylax water frogs is alarming and unveils their invasiveness, contributing therefore to explain their success of invasion over broad geographic scales