Axonal supercharged interpositional jump‐graft with a hybrid artificial nerve conduit containing adipose‐derived stem cells in facial nerve paresis rat model

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/146584/1/micr30389_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/146584/2/micr30389.pd

Pentosidine Accumulation in Human Oocytes and Their Correlation to Age-Related Apoptosis

Age-related atresia of ovarian follicles is characterized by apoptosis of the constituent cells. Recent studies have indicated that dysfunction of the proteasome and endoplasmic reticulum and subsequent apoptosis in the presence of oxidative stress have relevance to aging. The aim of this study was to assess the involvement of these processes in age-related follicular atresia. Formalin-fixed, paraffin-embedded sections of ovaries obtained at surgery from 74 women (age: 21–54 y) were examined with the terminal deoxynucleotidyl transferase-mediated, dUTP-biotin nick-end labeling (TUNEL) method and an immunohistochemical technique. Primary antibodies used in immunohistochemistry were against pentosidine, ubiquitin and caspase 12. Histological localization of these substances in oocytes was observed by light microscopy, and labeling indices of these cells were evaluated by regression analysis. Positive signals for pentosidine, ubiquitin, caspase 12, and TUNEL were detectable in oocytes of the primordial, primary and their atretic follicles. Regression analysis revealed an age-related increase in the labeling indices for pentosidine, ubiquitin, caspase 12, and TUNEL. These results suggest that pentosidine accumulation in human oocytes is related to apoptosis and increases with age. Further studies will be necessary to clarify the involvement of pentosidine accumulation, proteasome inhibition, and endoplasmic reticulum stress in age-related apoptosis of oocytes in human ovaries

Uma implementação do esquema de multi-assinaturas MuSig no cenário m-de-n com árvores de Merkle e suas aplicações ao bitcoin

Neste trabalho é proposto uma implementação prova de conceito do esquema de multi- assinatura MuSig no cenário m-de-n com validação de chaves públicas agregadas através de árvores de Merkle, expandindo a descrição original do MuSig neste cenário. Foi uti- lizado a linguagem de programação Python e curvas elípticas (com destaque para a curva secp256k1) para a construção da implementação. As multi-assinaturas produzidas man- têm o tamanho de uma assinatura individual de Schnorr e podem ser verificadas utilizando uma única chave pública agregada calculada a partir das chaves públicas dos signatarios, tal situação traz expectativas positivas quanto a melhorias no desempenho e privacidade do Bitcoin. Melhorias futuras incluem a implementação de uma prova de conceito que integre o MuSig diretamente ao protocolo Bitcoin e a utilização do MuSig para construção de um esquema de assinatura agregada interativa (IAS).This work presents a proof-of-concept implementation of the multi-signature scheme MuSig in the m-of-n scenario with aggregated key validation using Merkle trees, expanding the original MuSig description in this scenario. Python was the programming language of choice and elliptic curves (mainly secp256k1) were used as the basis of the implementa- tion. The multi-signatures generated by the scheme can keep the same size as a single Schnorr signature and can be verified with a single aggregated public key computed from the individual public keys of the signers, this scenario brings positive expectations for per- formance and privacy improvements in Bitcoin. Future works includes a proof-of-concept implementation that integrates the MuSig scheme directly into the Bitcoin protocol and the implementation of an interactive signature scheme (IAS) with the MuSig scheme as its basis