25 research outputs found
Views of the press and media strategies of Missouri state legislators: Statehouse reporter with the Jefferson City News Tribune
Professional project report submitted in partial fulfillment of the requirements for the degree of Masters of Arts in Journalism from the School of Journalism, University of Missouri--Columbia.During eight interviews at the Missouri state Capitol, state lawmakers discussed their strategies of working through the press to advance their policy priorities. The lawmakers, who came from across the state, employ a variety of strategies in dealing with the press. Some of the lawmakers use social media heavily to push their messages to followers, while others have no social media presence at all. The lawmakers articulated varying strategies for dealing with the press, ranging from targeting specific journalists with story tips to sending out press releases to a long list of reporters. The discussions also delved into critiques of the press and how those criticisms affected the way particular lawmakers approach their relationship with members of the media.Includes bibliographic references
Sumo-dependent substrate targeting of the SUMO protease Ulp1
<p>Abstract</p> <p>Background</p> <p>In the yeast <it>Saccharomyces cerevisiae</it>, the essential small ubiquitin-like modifier (SUMO) protease Ulp1 is responsible for both removing SUMO/Smt3 from specific target proteins and for processing precursor SUMO into its conjugation-competent form. Ulp1 localizes predominantly to nuclear pore complexes but has also been shown to deconjugate sumoylated septins at the bud-neck of dividing cells. How Ulp1 is directed to bud-neck localized septins and other cytoplasmic deconjugation targets is not well understood.</p> <p>Results</p> <p>Using a structure/function approach, we set out to elucidate features of Ulp1 that are required for substrate targeting. To aid our studies, we took advantage of a catalytically inactive mutant of Ulp1 that is greatly enriched at the septin ring of dividing yeast cells. We found that the localization of Ulp1 to the septins requires both SUMO and specific structural features of Ulp1's catalytic domain. Our analysis identified a 218-amino acid, substrate-trapping mutant of the catalytic domain of Ulp1, Ulp1(3)<sup>(C580S)</sup>, that is necessary and sufficient for septin localization. We also used the targeting and SUMO-binding properties of Ulp1(3)<sup>(C580S) </sup>to purify Smt3-modified proteins from cell extracts.</p> <p>Conclusions</p> <p>Our study provides novel insights into how the Ulp1 SUMO protease is actively targeted to its substrates <it>in vivo </it>and <it>in vitro</it>. Furthermore, we found that a substrate-trapping Ulp1(3)<sup>(C580S) </sup>interacts robustly with human SUMO1, SUMO2 and SUMO2 chains, making it a potentially useful tool for the analysis and purification of SUMO-modified proteins.</p
Affectus Hispaniae en la historiografía del Alto Imperio
This paper analyses texts written by Greek and Latin High Empire historians dealing with Hispania. Some of the authors have a very positive view (Florus, Iustinus, Appian) while others are clearly negative (Veleius Paterculus, Valerius Maximus) though most of them show little interest, indifference or variety of opinions. When there is interest in the region or praise, it is because the author comes from Hispania or he is trying to please an emperor born in Hispania, but it could also be due to a universal conception of history revealing a critical attitude towards Roman imperialism, as in Appian. The praise found in Iustinus’s epitome should be attributed to the author of the epitome rather than to Pompeius Trogus. This can be taken as evidence for situating Iustinus’s life and work in the 2nd century A.D. Loathing of Hispania seems to have its origins in conservative, ‘optimate’ nationalist circles, who perceive the province as the ‘popular’ region that acclaimed and welcomed ‘seditious’ individuals such as Tiberius Gracchus and Sertorius.Se estudian en este trabajo los textos de historiadores del Alto Imperio, latinos y griegos, que tratan sobre Hispania. En algunos autores encontramos una visión muy positiva (Floro, Justino, Apiano) y en otros claramente negativa (Veleyo Patérculo, Valerio Máximo), aunque en la mayoría de los casos hay escasa atención, indiferencia o diversidad de opiniones. El interés por la región y los elogios pueden estar motivados por el origen hispánico del autor o su voluntad de agradar a algún emperador oriundo de Hispania, pero también por una concepción universal de la historia que denota en ocasiones una posición crítica con el imperialismo romano, como es el caso de Apiano. La alabanza que hallamos en el epítome de Justino creemos que debe atribuirse más al epitomador que a Pompeyo Trogo, lo que apoyaría una datación temprana de la vida y la obra de Justino (s. II d.C.). La aversión hacia Hispania parece haber surgido en medios conservadores, “optimates” nacionalistas, que ven la provincia como el territorio “popular”, que encumbró y acogió a “sediciosos” como Tiberio Graco y Sertorio
The TESS-Keck Survey II: An Ultra-Short Period Rocky Planet and its Siblings Transiting the Galactic Thick-Disk Star TOI-561
We report the discovery of TOI-561, a multi-planet system in the galactic
thick disk that contains a rocky, ultra-short period planet (USP). This bright
() star hosts three small transiting planets identified in photometry
from the NASA TESS mission: TOI-561 b (TOI-561.02, P=0.44 days, ), c (TOI-561.01, P=10.8 days,
), and d (TOI-561.03, P=16.3 days,
). The star is chemically ([Fe/H],
[/H]) and kinematically consistent with the galactic
thick disk population, making TOI-561 one of the oldest (Gyr) and
most metal-poor planetary systems discovered yet. We dynamically confirm
planets b and c with radial velocities from the W. M. Keck Observatory High
Resolution Echelle Spectrometer. Planet b has a mass and density of
and gcm, consistent with
a rocky composition. Its lower-than-average density is consistent with an
iron-poor composition, although an Earth-like iron-to-silicates ratio is not
ruled out. Planet c is and gcm,
consistent with an interior rocky core overlaid with a low-mass volatile
envelope. Several attributes of the photometry for planet d (which we did not
detect dynamically) complicate the analysis, but we vet the planet with
high-contrast imaging, ground-based photometric follow-up and radial
velocities. TOI-561 b is the first rocky world around a galactic thick-disk
star confirmed with radial velocities and one of the best rocky planets for
thermal emission studies.Comment: Accepted at The Astronomical Journal; 25 pages, 10 figure
SAS model for competition by EOI
SAS code used to analyze outcome of cross-streak data by environment of isolatio
SAS model for competition by inhibition
SAS code used to analyze outcome of cross-streak data by pyocin inhibitio
Data from: Life in the cystic fibrosis upper respiratory tract influences competitive ability of the opportunistic pathogen Pseudomonas aeruginosa
Understanding characteristic differences between host-associated and free-living opportunistic pathogens can provide insight into the fundamental requirements for success after dispersal to the host environment, and more generally into the ecological and evolutionary processes by which populations respond to simultaneous selection on complex interacting traits. We examined how cystic fibrosis (CF) associated and environmental isolates of the opportunistic pathogen Pseudomonas aeruginosa differ in the production of an ecologically important class of proteinaceous toxins known as bacteriocins, and how overall competitive ability depends on the production of and resistance to these bacteriocins. We determined bacteriocin gene content in a diverse collection of environmental and CF isolates and measured bacteriocin-mediated inhibition, resistance, and the outcome of competition in a shared environment between all possible pairs of these isolates at 25 °C and 37 °C. Although CF isolates encoded significantly more bacteriocin genes, our phenotypic assays suggest that they have diminished bacteriocin-mediated killing and resistance capabilities relative to environmental isolates, regardless of incubation temperature. Notably, however, although bacteriocin killing and resistance profiles significantly predicted head to head competitive outcomes, CF and environmental isolates did not differ significantly in their competitive ability. This suggests that the contribution of bacteriocins to competitive ability involves selection on other traits that may be pleiotropically linked to interference competition mediated by bacteriocins