Papel etiológico de los virus en la enfermedad periodontal

El objetivo de esta revisión es presentar la evidencia disponible que relaciona la infección por virus con el desarrollo de periodontitis. Esta relación se ha visto con los virus de la familia herpes, sobretodo el citomegalovirus humano (CMV) y el virus Epstein-Barr (VEB), así como con el virus de la inmunodeficiencia humana (HIV). Las infecciones por herpesvirus generalmente sucede en dos fases, durante la primoinfección la clínica suele ser leve o asintomática y a esta le sigue una fase asintomática en la que el virus se encuentra en estado de latencia. Dicho estado se verá interrumpido esporádicamente por periodos de activación en los que se produce una replicación viral y posiblemente se dé una manifestación de la enfermedad que explicaría, en parte, el progreso en episodios de la enfermedad periodontal. De hecho, algunas de las causas que llevan a la reactivación del virus también se consideran factores de riesgo de la enfermedad periodontal y podrían relacionar a ambas patologías. The purpose of this review is to evaluate the evidence supporting the hypothesis that viral infection plays a role in the development of periodontitis. This relationship has been found mainly with the herpesvirus family, especially with human cytomegalovirus (CMV) and with Epstein-Barr virus (EBV), but also with human immunodeficiency virus (HIV). The herpesvirus infection generally involves a mild or asymptomatic primary phase followed by an asymptomatic latent phase interrupted sporadically by periods of activation, where viral replication and possibly clinical disease become manifest and which will in part, explain the episodic progressive nature of human periodontitis. In fact, herpesvirus reactivation is triggered by a number of immunosuppressing factors, some of which have also been shown to be risk indicators of periodontal disease and which could relate both patologies

Petrological and chronological considerations on the hercynian granitic rocks from Galicia (NW Spain)

[Resumen] Se realiza un estudio sobre más de 600 análisis químicos correspondientes a los diversos grupos de granitoides hercínicos de Galicia, y se establecen valores medios representativos para la composición química mayoritaria de estos grupos. La comparación con las medias previamente establecidas para el N. O. de Galicia, permite observar un desplazamiento hacia términos composicionales más ácidospara el conjunto de los granitoides sincinemáticos más profundos, que resulta notablemente más acentuado en el grupo de los granitoides sincinemáticos predominantemente biotíticos. Para los granitoides postcinemáticos, las desviaciones observadas son en cambio poco significativas. El examen de los datos normativos medios, pone en evidencia un carácter peralumínico generalizado para todo el conjunto, apreciándose no obstante un drástico descenso en el contenido en corindón para los granitoides postcinemáticos, que marca una distribución bimodal para este componente normativo. La clasificación petrológica con base en la mineralogía normativa pone de manifiesto un espectro composicional que fluctúa esencialmente entre granitos y monzogranitos, con muy escasa representación de los términos granodior1ticos. La variabilidad observada para los elementos mayores más signiflcativos presenta pautas poco dispersivas. Los espectros de tierras raras tienen una configuración muy similar para los granitoides precoces predominantemente biotíticos, los granitos de dos micas sincinemáticos y los granitoides inhomogéneos, con anomalías negativas de Eu poco acentuadas. Por el contrario, los granitoides tardicinemáticos someros y los granitoides post-cinemáticos, presentan también espectros bastante similares entre sí, con anomalís negativas de Eu que pueden ser considerables. Las dataciones cronológicas efectuadas por el método de Rb-Sr en roca total en algunos de los plutones post-cinemáticos se encuentran en un intervalo aproximado de 275-287 m. a., mientras que la mayoría de las dataciones sobre unidades sincinemáticas de dos micas y predominantemente biotíticas fluctúan entre 295 y 323 m. a., aunque se encuentran algunos datos fuera de este entorno, que precisan futuras verificaciones..[Abstract] A survey over more than 600 chemical analysis of Hercynian granitic rocks from Galicia is presented, and a new set of representative mean compositional values of the main groups is calculated. In general, these new mean values are richer in Si02 than the previously available ones. This difference is more conspicous in the biotite rich synkinematic group, and is minium in the postkynematic granitoids. The average normative compostion, shows a systematic peraluminous character for aH the granitoids. However, the group of postkynematic granitoids, are poorer in normative corundum than the other groups, which results in a bimodal distribution of this normative component. The normative petrological c1assification shows a compositional spectrúm ranging mainly from granites to monzogranites. The Granodioritic rocks are very scarce. The variation trends for the main mayor elements display patterns with little dispersion. The REE spectra for the biotite rich synkinematic granites, two mica synkinematic granites and inhomogenous granites are very similar, and show negligible Eu anomalies. By contrast, the shaHow level intrusion late-kynematic and postkynematic granites, display conspicous Eu anomalies and have similar spectra patterns, but different from the previously referred ones. Whole-rock-Rb-Sr ages for the postkynematic plutons range from 287 to 275 M. y. The majority of the data the synkinematic units range from 323 to 295 M. y. However, sorne chronological data faH out of these intervals, showing the need of further studies

Analysis of Plasminogen Genetic Variants in Multiple Sclerosis Patients

Dessa Sadovnick, A.; Alcina, Antonio; Fedetz, María; Matesanz, F.; Vilariño-Güell, Carles; Dessa Sadovnick, A. et. al.Multiple sclerosis (MS) is a prevalent neurological disease of complex etiology. Here, we describe the characterization of a multi-incident MS family that nominated a rare missense variant (p.G420D) in plasminogen (PLG) as a putative genetic risk factor for MS. Genotyping of PLG p.G420D (rs139071351) in 2160 MS patients, and 886 controls from Canada, identified 10 additional probands, two sporadic patients and one control with the variant. Segregation in families harboring the rs139071351 variant, identified p.G420D in 26 out of 30 family members diagnosed with MS, 14 unaffected parents, and 12 out of 30 family members not diagnosed with disease. Despite considerably reduced penetrance, linkage analysis supports cosegregation of PLG p.G420D and disease. Genotyping of PLG p.G420D in 14446 patients, and 8797 controls from Canada, France, Spain, Germany, Belgium, and Austria failed to identify significant association with disease (P = 0.117), despite an overall higher prevalence in patients (OR = 1.32; 95% CI = 0.93–1.87). To assess whether additional rare variants have an effect on MS risk, we sequenced PLG in 293 probands, and genotyped all rare variants in cases and controls. This analysis identified nine rare missense variants, and although three of them were exclusively observed in MS patients, segregation does not support pathogenicity. PLG is a plausible biological candidate for MS owing to its involvement in immune system response, blood-brain barrier permeability, and myelin degradation. Moreover, components of its activation cascade have been shown to present increased activity or expression in MS patients compared to controls; further studies are needed to clarify whether PLG is involved in MS susceptibility.We also thank Généthon, L’Association Française contre les Myopathies (AFM), la Fondation pour l’Aide à la Recherche sur la Sclérose en Plaques (ARSEP), and the Biological Resources Centre (BRC) of The French Multiple Sclerosis Genetics Group (CRB-REFGENSEP). This research was undertaken thanks to funding from the Canada Research Chair [950-228408] and Canada Excellence Research Chair programs [214444], Canadian Institutes of Health Research [MOP-137051], Vancouver Coastal Health Research Institute, the Milan & Maureen Ilich Foundation [11-32095000], and the Vancouver Foundation [ADV14-1597]. Replication studies received funding from the program “Investissements d’avenir” ANR-10-IAIHU-06. Fondo de Investigación Sanitaria (FIS)-Instituto de Salud Carlos III (ISCIII)-Fondos Europeos de Desarrollo Regional (FEDER), Unión Europea [grant numbers P12/00555, PI13/01527, PI13/01466 and PI13/0879 to F.M., A.A. and G.I.] and Junta de Andalucía -FEDER [grant number CTS2704 to F.M.]. B.D. is a Clinical Investigator of the Research Foundation Flanders (FWO-Vlaanderen). A.G. and B.D. are supported by the Research Fund KU Leuven (OT/11/087 and CREA/14/023) and the Research Foundation Flanders (G073415N). A.L.T. reports personal fees from Biogen Idec, Chugai, Medimmune, Teva Innovation, and EMD Serono, and grants and personal fees from Genzyme Sanofi and Roche.Peer reviewe

Petrological and geochronological study of the postkinematic pluton of Neira (Lugo, Spain)

[Resumen] El plutón granítico de Neira intruye discordantemente en metasedimentos precámbricos y cámbricos y en granitoides sincinemáticos de dos micas, con posterioridad al desarrollo de las fases deformativas hercínicas principales. En este macizo plutónico se distinguen dos unidades intrusivas principales y el espectro litológico fundamental fluctua entre granodioritas biotíticas y leucogranitos moscovíticos-biotíticos, con predominio de las variedades graníticas (adamellíticas). Estos términos petrol6gicos están relacionados entre sí a través de un proceso evolutivo controlado fundamentalmente por cristalización fraccionada, desempeñando un importante papel los minerales accesorios. La datación de esta unidad plutónica por el procedimiento Rb-Sr en roca total, proporciona una edad de 287 ± 5 Ma, con una relaci6n inicial 87 Sr /86 Sr de 0.7074 ± 0.0004, que corresponde a un episodio intrusivo Estefaniense[Abstract] Neira granitic pluton intrudes in cambrian and precambrian metasediments and in synkinematic two mica granites after the development of the two main hercynian tectonic phases. Two intrusive units are distinguished in this pluton and its petrological composition ranges from biotitic granodiorites to two mica leucogranites, predominating biotitic adamellites. This suite of rocks is related by an evolution process mainly ruled by crystal fractionation. The accesory minerals play an important role in this process. Rb-Sr dating of this plutonic unit gives and age of 287 ± 5 M y and an initial 87 Sr/86 Sr = 0.7074 ± 0.0004

IL2RA/CD25 Gene Polymorphisms: Uneven Association with Multiple Sclerosis (MS) and Type 1 Diabetes (T1D)

[Background] IL-2 receptor (IL2R) alpha is the specific component of the high affinity IL2R system involved in the immune response and in the control of autoimmunity. [Methods and Results] Here we perform a replication and fine mapping of the IL2RA gene region analyzing 3 SNPs previously associated with multiple sclerosis (MS) and 5 SNPs associated with type 1 diabetes (T1D) in a collection of 798 MS patients and 927 matched Caucasian controls from the south of Spain. We observed association with MS in 6 of 8 SNPs. The rs1570538, at the 3′- UTR extreme of the gene, previously reported to have a weak association with MS, is replicated here (P = 0.032). The most associated T1D SNP (rs41295061) was not associated with MS in the present study. However, the rs35285258, belonging to another independent group of SNPs associated with T1D, showed the maximal association in this study but different risk allele. We replicated the association of only one (rs2104286) of the two IL2RA SNPs identified in the recently performed genome-wide association study of MS. [Conclusions] These findings confirm and extend the association of this gene with MS and reveal a genetic heterogeneity of the associated polymorphisms and risk alleles between MS and T1D suggesting different immunopathological roles of IL2RA in these two diseases.Financial support for the study was provided by the Ministerio de Educación y Ciencia (grants PN-SAF2006-02023 and TIN2007-67418-C03-03) and Junta de Andalucía (P07-CVI-02551) to A. Alcina and Servicio Andaluz de Salud de la Junta de Andalucía (grant PI0168/2007) to F. Matesanz. María Fedetz is a holder of a fellowship from Fundación IMABIS. Dorothy Ndagire is a holder of AECI-Ministerio de Asuntos Exteriores fellowship

Inflammation Determines the Pro-Adhesive Properties of High Extracellular D-Glucose in Human Endothelial Cells In Vitro and Rat Microvessels In Vivo

Background: Hyperglycemia is acknowledged as an independent risk factor for developing diabetes-associated atherosclerosis. At present, most therapeutic approaches are targeted at a tight glycemic control in diabetic patients, although this fails to prevent macrovascular complications of the disease. Indeed, it remains highly controversial whether or not the mere elevation of extracellular D-glucose can directly promote vascular inflammation, which favors early proatherosclerotic events. Methods and Findings: In the present work, increasing extracellular D-glucose from 5.5 to 22 mmol/L was neither sufficient to induce intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) expression, analyzed by flow cytometry, nor to promote leukocyte adhesion to human umbilical vein endothelial cells (HUVEC) in vitro, measured by flow chamber assays. Interestingly, the elevation of D-glucose levels potentiated ICAM-1 and VCAM-1 expression and leukocyte adhesion induced by a pro-inflammatory stimulus, such as interleukin (IL)-1b (5 ng/mL). In HUVEC, high D-glucose augmented the activation of extracellular signal-regulated kinase 1/2 (ERK 1/2) and nuclear transcription factor-kB (NF-kB) elicited by IL-1b, measured by Western blot and electromobility shift assay (EMSA), respectively, but had no effect by itself. Both ERK 1/2 and NF-kB were necessary for VCAM-1 expression, but not for ICAM-1 expression. In vivo, leukocyte trafficking was evaluated in the rat mesenteric microcirculation by intravital microscopy. In accordance with the in vitro data, the acute intraperitoneal injection of D-glucose increased leukocyte rolling flux, adhesion and migration, but only when IL-1b was coadministered. Conclusions: These results indicate that the elevation of extracellular D-glucose levels is not sufficient to promote vascular inflammation, and they highlight the pivotal role of a pro-inflammatory environment in diabetes, as a critical factor conditioning the early pro-atherosclerotic actions of hyperglycemia

Uncontrolled Donation after Circulatory Death: European practices and recommendations for the development and optimization of an effective programme.

Shortage of organs has made a global interest for donation after circulatory death (DCD) to re-emerge. While controlled DCD (cDCD) has been progressively increasing, uncontrolled DCD (uDCD) has only been developed in a few settings.1 This activity is quantitatively important in France and Spain, although it has also been reported in other European countries, as Austria, Belgium, Italy, the Netherlands, and recently in Russia.2,3 uDCD protocols have allowed the transplantation of a significant number of kidneys, livers and lungs at these countries.3 Excellent graft survival has been reported in kidney transplantation from uDCD, in spite of an increased incidence of delayed graft function (DGF).4,5,6,7,8,9,10,11,12,13,14,1516 Albeit promising, results with liver transplants obtained in uDCD protocols do not consistently provide similar outcomes compared with livers from donors after brain death (DBD), mainly due to a higher incidence of primary graft dysfunction and non-function and biliary complications.17,18,19,20,21,22 Lung transplantation is still facing limited experience, but preliminary results are encouraging.pre-print938 K

Numerical Reconstruction of Ejector Rocket Experimental Tests

Air ejector rocket systems, typical of combined cycle engines for space propulsion applications, have been studied within the ESA Future European Space Transportation Investigations Program. The description and validationof the computational fluid dynamics (CFD) algorithm that has been tuned to simulate the behavior of these systems, and the numerical rebuilding of the ejector rocket experimental tests that were carried out at TNO in The Netherlands are given. The computational developments being presented target the problem of turbulent mixing layer simulation, which is one of the leading phenomena that govern flow behavior inside an ejector rocket. Comparison between experimental and CFD data is given for two validation test cases: a two-dimensional turbulent mixing layer and an axysimmetric ejector in cold flow. Then, the numerical rebuilding of the ejector rocket experimental tests is presented, and the results are discussed with regard to the comparison between numerical and experimental data

The impact of donor policies in Europe: a steady increase, but not everywhere

<p>Abstract</p> <p>Background</p> <p>Transplantable organs are scarce everywhere. Therefore, countries have developed policies to support the efficient use of potential donors. Nevertheless, the shortage of organs remains. Were these policies in vain? The aim of this study is to assess the impact of donor policies on donor procurement in 10 Western European countries from 1995 to 2005.</p> <p>Method</p> <p>To assess the impact of the donor policies we studied the conversion of potential donors into effectuated donors. 80% of the donors died from CVAs or a (traffic) accident. We considered these mortality rates to be a good proxy for potential donors. Here we call the conversion of potential donors into actual donors 'the donor efficiency rate by proxy'.</p> <p>Results</p> <p>The mortality rates for CVA and (traffic) accidents have decreased in the countries under study. At the same time, in most countries the donor efficiency rates have steadily increased. The variance in donor efficiency rates between countries has also increased from 1995 to 2005. Four countries introduced a new consent system or changed their existing system, without (visible) long-term effects.</p> <p>Conclusion</p> <p>The overall increase in donor efficiency means that the efforts to improve donor policies have paid off. However, substantial differences between countries were found. The success of donor policies in terms of the number of absolute donors is blurred by the success of policies on traffic safety and CVA treatment. It remains unclear which specific policy measures are responsible for the increase in donor efficiency rates. This increase is not related to having a presumed consent system. Furthermore, an analysis of countries that introduced a new consent system or changed their system showed no effect on donor efficiency.</p

Detection of displacements on Tenerife Island, Canaries, using radar interferometry

Tenerife is one of the most well monitored islands of the Canaries, but the surveillance generally is centred on Las Canadas Caldera, where the Teide volcano is located. In the last 180 000 yr, the eruptions on Tenerife Island have never occurred in the same volcanic structure, except for the Teide and Pico Viejo central volcanic system, so that a complete monitoring network would have to cover the whole island. As a result, Synthetic Aperture Radar Interferometry (InSAR) is being used on Tenerife, because this space technique can provide a displacement map of the surface of the earth with centimetre precision. This paper presents the results obtained on Tenerife Island using 18 SAR images acquired by the ERS-1 and ERS-2 satellites during the period 1992-2000. Two important results have been obtained: no deformation on Las Canadas Caldera, coinciding with results obtained using terrestrial techniques, and two subsidence episodes outside monitoring areas in the NW of the island, in the region of the last historic eruptions. These results show that InSAR is a useful technique for monitoring the entire island, thus allowing us to discover deformations in areas that are not routinely or easily monitored. This technique has been used in combination with Global Positioning System (GPS) observation of a global network on the island to define a new geodetic monitoring system. The possible causes of the deformations observed have been studied in an endeavour to discern if they might be of natural origin, in particular linked to a reactivation of prior volcanic activity. Examination of the geophysical observations on the island, human activities underway and the results of the modelling seem to indicate that at least part of the deformations may be caused by changes in the groundwater level and therefore are not linked to a volcanic reactivation. This result is important because it implies that, if geodetic volcano monitoring is to be performed on the island, the system used must be capable of discerning between various possible origins of the deformation by analysing their patterns and ancillary information from other sources. In this regard, InSAR is a basic tool on account of its unpaired wide area coverage and spatial density