Fine Scale Determinants of Soil Litter Fauna on a Mediterranean Mixed Oak Forest Invaded by the Exotic Soil-Borne Pathogen Phytophthora cinnamomi

16 páginas.-- 5 figuras.-- 1 tablas.-- 56 referencias.-- This article belongs to the Special Issue "Ecology and Management of Invasive Species in Forest Ecosystems".There is growing recognition of the importance of soil fauna for modulating nutrient cycling processes such as litter decomposition. However, little is known about the drivers promoting changes in soil fauna abundance on a local scale. We explored this gap of knowledge in a mixed oak forest of Southern Spain, which is under decline due to the invasion of the exotic soil-borne pathogen Phytophthora cinnamomi. Meso-invertebrate abundance found in soil litter was estimated at the suborder level. We then explored their statistical correlations with respect to light availability, tree and litter characteristics, and P. cinnamomi abundance. Oribatida and Entomobryomporpha were the most abundant groups of Acari and Collembola, respectively. According to their trophic level, predator and detritivore abundances were positively correlated while detritivores were, in turn, positively correlated with pathogen abundance and negatively influenced by light availability and tree defoliation. These overall trends differed between groups. Among detritivores, Diplopoda preferred highly decomposed litter while Oribatida and Psocoptera preferred darker environments and Poduromorpha were selected for environments with lower tree defoliation. Our results show the predominant role of light availability in influencing litter fauna abundances at local scales and suggest that the invasive soil-borne pathogen P. cinnamomi is integrated in these complex relationships.O.G. acknowledges postdoctoral financial support provided by the European Union Horizon research and innovation program under the Marie Sklodowska-Curie grant agreement No 661118-BioFUNC. O. G. also thanks research support from the Spanish Ministry of Economy and Competitiveness through the IMPLANTIN project (CGL2015-65346-R). L.G.A. acknowledges support from the MICINN project INTERCAPA (CGL-2014-56739-R)

Estudio comparativo entre la percepción de la intensidad del dolor por parte del personal médico y la intensidad del dolor obtenida a través de la aplicación de la escala cara, piernas, actividad, llanto y consuelo (face, legs, activity, crying and consolability) en niños de 1 a 5 años que acuden con este síntoma al Hospital Pediátrico Baca Ortiz

Se define el dolor como toda aquella experiencia emocional y sensorial desagradable asociada a un daño tisular real o potencial. En la edad pediátrica la valoración del grado de dolor es difícil debido a su dificultad para expresarse o la falta de capacidad de comprensión que tienen para identificar el mismo, ya que, además de ser un fenómeno biológico se ve influenciado también por aspectos psicológicos y del entorno infantil que repercuten y pueden provocar reacciones dolorosas diferentes.   El control del dolor es un tema vital en cualquier servicio de emergencias pediátricas

An asymmetrical bulk-modified composite MOS transistor with enhanced linearity

In this work, an asymmetrical bulk-linearized composite MOSFET is presented, with an enhanced linear range and an equivalent saturation voltage of up to several hundred mV even in weak inversion, allowing to implement large MOS resistors. Some preliminary measurements are presented, as well as 150MΩ and 200MΩ equivalent resistors simulations, with a linear range up to 1.5V. A low frequency, 40dB gain, fully integrated cardiac sensing channel filter/amplifier is also shown. Taking advantage of the proposed technique, the circuit consumes only 25nA of supply current.Agencia Nacional de Investigación e Innovació

Sensitivity of the earthquake response of a HDRB seismically isolated hospital to earthquakes on crustal faults

: Seismicity in Chile is strongly dominated by subduction megathrust events. Hence, the characteristics of ground motions other than those produced by these events are less known. Such is the case for the ground motions generated by crustal faults close to urban areas; one example is the San Ramon fault (SRF), which was recently declared as potentially active. This work addresses the sensitivity of the structural response of a High Damping Rubber Bearing (HDRB) seismically isolated hospital in downtown Santiago, Chile, to earthquakes generated on this Fault. The SRF lies at less than 15 kilometers away from the hospital and there is very little data on its seismogenic capacity and source parameters. Hence, physics-based synthetic earthquake simulations using front-end technologies and bounds for the source parameters are used to predict the impact of such event in the performance of this test structure. The earthquake source parameters considered as uncertain in this study are the event magnitude, average rupture velocity, and corner frequency. To minimize the number of parameter combinations, a median, an upper, and a lower bound value were selected for each parameter. A set of 16 synthetic ground motion realizations were generated for each parameter combination (27), leading to a total of 432 synthetic records for the analysis. The variability of these records, and the inelastic cyclic response of the HDRBs, are used to compute the expected variations in the earthquake response of the hospital. Building response parameters, such as maximum deformation and shear forces of the isolated layer, inter-story building drifts, and floor accelerations of the superstructure are used to characterize variability of the hospital response. It is concluded that despite the smaller magnitudes of crustal events relative to the subduction ones, they will control some of the response limit states for buildings closer than 15 km to the fault, such as this seismically isolated hospital

A phase I clinical study of vaccination of melanoma patients with dendritic cells loaded with allogeneic apoptotic/necrotic melanoma cells. Analysis of toxicity and immune response to the vaccine and of IL-10 -1082 promoter genotype as predictor of disease progression

<p>Abstract</p> <p>Background</p> <p>Sixteen melanoma patients (1 stage IIC, 8 stage III, and 7 stage IV) were treated in a Phase I study with a vaccine (DC/Apo-Nec) composed of autologous dendritic cells (DCs) loaded with a mixture of apoptotic/necrotic allogeneic melanoma cell lines (Apo-Nec), to evaluate toxicity and immune responses. Also, IL-10 1082 genotype was analyzed in an effort to predict disease progression.</p> <p>Methods</p> <p>PBMC were obtained after leukapheresis and DCs were generated from monocytes cultured in the presence of GM-CSF and IL-4 in serum-free medium. Immature DCs were loaded with gamma-irradiated Apo-Nec cells and injected id without adjuvant. Cohorts of four patients were given four vaccines each with 5, 10, 15, or 20 × 10⁶DC/Apo-Nec cell per vaccine, two weeks apart. Immune responses were measured by ELISpot and tetramer analysis. Il-10 genotype was measured by PCR and corroborated by IL-10 production by stimulated PBMC.</p> <p>Results</p> <p>Immature DCs efficiently phagocytosed melanoma Apo-Nec cells and matured after phagocytosis as evidenced by increased expression of CD83, CD80, CD86, HLA class I and II, and 75.2 ± 16% reduction in Dextran-FITC endocytosis. CCR7 was also up-regulated upon Apo-Nec uptake in DCs from all patients, and accordingly DC/Apo-Nec cells were able to migrate <it>in vitro </it>toward MIP-3 beta. The vaccine was well tolerated in all patients. The DTH score increased significantly in all patients after the first vaccination (Mann-Whitney Test, p < 0.05). The presence of CD8⁺T lymphocytes specific to gp100 and Melan A/MART-1 Ags was determined by ELISpot and tetramer analysis in five HLA-A*0201 patients before and after vaccination; one patient had stable elevated levels before and after vaccination; two increased their CD8 + levels, one had stable moderate and one had negligible levels. The analysis of IL-10 promoter -1082 polymorphism in the sixteen patients showed a positive correlation between AA genotype, accompanied by lower <it>in vitro </it>IL-10 production by stimulated PBMC, and faster melanoma progression after lymph nodes surgery (p = 0.04). With a mean follow-up of 49.5 months post-surgery, one stage IIC patient and 7/8 stage III patients remain NED but 7/7 stage IV patients have progressed.</p> <p>Conclusion</p> <p>We conclude that DC/Apo-Nec vaccine is safe, well tolerated and it may induce specific immunity against melanoma Ags. Patients with a low-producing IL-10 polymorphism appear to have a worst prognosis.</p> <p>Trial registration</p> <p>Clinicaltrials.gov (NHI) NCT00515983</p

A RISC-V based medical implantable SOC for high voltage a current tissue stimulus

A RISC-V based System on Chip (SoC) for high voltage and current tissue stimulus, targeting implantable medical devices, is presented. The circuit is designed in a 0.18μm HV-CMOS process, including the RISC-V 32RVI based microcontroller core, called Siwa —which includes SPI, UART and GPIO interfaces, a packet-based bus and memory controller, and 8kB SRAM—, combined with several biological tissue stimulus and sensing circuits. The complete test chip (analog+RISC-V) occupies a 5mm2 area but only 0.82mm2 correspond to the RISCV micro-controller, which operates up to 20MHz, with average energy needs of less than 48 pJ/cycle (3pJ STD), and for which several reliability and safety issues were considered.Agencia Nacional de Investigación e Innovació

Siwa: a RISC-V RV32I based micro-controller for implantable medical applications

The design of Siwa1, a compact low power custom system on chip (SoC), targeted for implantable/wearable applications, is reported in this paper. Siwa is based on a RISC-V RV32I architecture. It has a centrally controlled non-pipelined structure, and it includes a control interface for an integrated sensing and stimulation device for biological tissues as well as standard communication interfaces. Siwa was developed from scratch using System Verilog, and implemented in a 180nm CMOS technology; Siwa includes a latch based register file c apable to read and write in one clock cycle with an area 30% smaller and a power consumption 25% lower with respect to an equivalent flip flop implementation; also, it has an estimated average power consumption of 70μW (48pJ/cycle) which is comparable to other micro-controllers commonly used in IMD applications.Agencia Nacional de Investigación e Innovació

Siwa: A custom RISC-V based system on chip (SOC) for low power medical applications

This work introduces the development of Siwa, a RISC-V RV32I 32-bit based core, intended as a flexible control platform for highly integrated implantable biomedical applications, and implemented on a commercial 0.18 m high voltage (HV) CMOS technology. Simulations show that Siwa can outperform commercial micro-controllers commonly used in the medical industry as control units for implantable devices, with energy requirements below the 50 pJ per clock cycle.Agencia Nacional de Investigación e Innovació

Generalized Lymphatic Anomaly as a Differential Diagnosis of Lytic Lesions

Generalized lymphatic anomaly (GLA) is an infrequent multiorgan disease characterized by the presence of abnormal proliferation of lymphatic vessels. The diagnosis requires histological confirmation, and the treatment is controversial. We are presenting a case of a 28-year-old male patient who was diagnosed with an extragonadal mediastinal nonseminomatous germ cell tumor. He underwent chemotherapy, and during this treatment, radiologic findings evidenced lytic lesions. Multiple biopsies were performed, which revealed the presence of abnormal lymphatic vessels, characteristic of GLA. There are different etiologies of osteolytic lesions, and on some occasions, they mimic a tumoral entity. The clinical suspicion of GLA is the first step in approaching the diagnosis, particularly in young adult patients

Database of spatial distribution of non indigenous species in Spanish marine waters

Research in marine Spanish waters are focused on several actions to achieve an effectively management on protected areas, with the active participation of the stakeholders and research as basic tools for decision-making. Among these actions, there is one about the knowledge and control on NIS. One of its objectives is the creation of NIS factsheets, which are going to be added to the National Marine Biodiversity Geographical System (GIS) providing complementary information about taxonomic classification, common names, taxonomic synonyms, species illustrations, identification morphological characters, habitat in the native and introduced regions, biological and ecological traits, GenBank DNA sequences, world distribution, first record and evolution in the introduced areas, likely pathways of introduction, effects in the habitats and interaction with native species, and potential management measures to apply. The database will also provide data for (1) the European online platforms, (2) the environmental assessment for the Descriptor 2 (D2-NIS) of the EU Marine Strategy Framework Directive (MSFD), as well as (3) supporting decisions made by stakeholders. It is the result of extensive collaboration among scientist, manager’s and citizen science in the Spanish North-Atlantic, South-Atlantic, Gibraltar Strait-Alboran, Levantine-Balearic and Canary Islands marine divisions, providing an updated overview of the spatial distribution of relevant extended and invasive NIS of recent and established NIS introduced by maritime transport and aquaculture pathways, as well as on cryptogenic or native species in expansion due to the climatic water warming trend