1,259 research outputs found
Protein kinase N2 regulates AMP kinase signaling and insulin responsiveness of glucose metabolism in skeletal muscle
Insulin resistance is central to the development of type 2 diabetes and related metabolic disorders. Because skeletal muscle is responsible for the majority of whole body insulin-stimulated glucose uptake, regulation of glucose metabolism in this tissue is of particular importance. Although Rho GTPases and many of their affecters influence skeletal muscle metabolism, there is a paucity of information on the protein kinase N (PKN) family of serine/threonine protein kinases. We investigated the impact of PKN2 on insulin signaling and glucose metabolism in primary human skeletal muscle cells in vitro and mouse tibialis anterior muscle in vivo. PKN2 knockdown in vitro decreased insulin-stimulated glucose uptake, incorporation into glycogen, and oxidation. PKN2 siRNA increased 5′-adenosine monophosphate-activated protein kinase (AMPK) signaling while stimulating fatty acid oxidation and incorporation into triglycerides and decreasing protein synthesis. At the transcriptional level, PKN2 knockdown increased expression of PGC-1α and SREBP-1c and their target genes. In mature skeletal muscle, in vivo PKN2 knockdown decreased glucose uptake and increased AMPK phosphorylation. Thus, PKN2 alters key signaling pathways and transcriptional networks to regulate glucose and lipid metabolism. Identification of PKN2 as a novel regulator of insulin and AMPK signaling may provide an avenue for manipulation of skeletal muscle metabolism
Genome-wide DNA methylation changes in a mouse model of infection-mediated neurodevelopmental disorders
Background
Prenatal exposure to infectious or inflammatory insults increases the risk of neurodevelopmental disorders. Using a well-established mouse model of prenatal viral-like immune activation, we examined whether this pathological association involves genome-wide DNA methylation differences at single nucleotide resolution.
Methods
Prenatal immune activation was induced by maternal treatment with the viral mimetic polyriboinosinic-polyribocytidylic acid in middle or late gestation. Following behavioral and cognitive characterization of the adult offspring (n = 12 per group), unbiased capture array bisulfite sequencing was combined with subsequent matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and quantitative real-time polymerase chain reaction analyses to quantify DNA methylation changes and transcriptional abnormalities in the medial prefrontal cortex of immune-challenged and control offspring. Gene ontology term enrichment analysis was used to explore shared functional pathways of genes with differential DNA methylation.
Results
Adult offspring of immune-challenged mothers displayed hyper- and hypomethylated CpGs at numerous loci and at distinct genomic regions, including genes relevant for gamma-aminobutyric acidergic differentiation and signaling (e.g., Dlx1, Lhx5, Lhx8), Wnt signaling (Wnt3, Wnt8a, Wnt7b), and neural development (e.g., Efnb3, Mid1, Nlgn1, Nrxn2). Altered DNA methylation was associated with transcriptional changes of the corresponding genes. The epigenetic and transcriptional effects were dependent on the offspring\u2019s age and were markedly influenced by the precise timing of prenatal immune activation.
Conclusions
Prenatal viral-like immune activation is capable of inducing stable DNA methylation changes in the medial prefrontal cortex. These long-term epigenetic modifications are a plausible mechanism underlying the disruption of prefrontal gene transcription and behavioral functions in subjects with prenatal infectious histories
Polynomial growth of volume of balls for zero-entropy geodesic systems
The aim of this paper is to state and prove polynomial analogues of the
classical Manning inequality relating the topological entropy of a geodesic
flow with the growth rate of the volume of balls in the universal covering. To
this aim we use two numerical conjugacy invariants, the {\em strong polynomial
entropy } and the {\em weak polynomial entropy }. Both are
infinite when the topological entropy is positive and they satisfy
. We first prove that the growth rate of the volume of
balls is bounded above by means of the strong polynomial entropy and we show
that for the flat torus this inequality becomes an equality. We then study the
explicit example of the torus of revolution for which we can give an exact
asymptotic equivalent of the growth rate of volume of balls, which we relate to
the weak polynomial entropy.Comment: 22 page
Exponential and moment inequalities for U-statistics
A Bernstein-type exponential inequality for (generalized) canonical
U-statistics of order 2 is obtained and the Rosenthal and Hoffmann-J{\o}rgensen
inequalities for sums of independent random variables are extended to
(generalized) U-statistics of any order whose kernels are either nonnegative or
canonicalComment: 22 page
Sparsity and Incoherence in Compressive Sampling
We consider the problem of reconstructing a sparse signal from a
limited number of linear measurements. Given randomly selected samples of
, where is an orthonormal matrix, we show that minimization
recovers exactly when the number of measurements exceeds where is the number of
nonzero components in , and is the largest entry in properly
normalized: . The smaller ,
the fewer samples needed.
The result holds for ``most'' sparse signals supported on a fixed (but
arbitrary) set . Given , if the sign of for each nonzero entry on
and the observed values of are drawn at random, the signal is
recovered with overwhelming probability. Moreover, there is a sense in which
this is nearly optimal since any method succeeding with the same probability
would require just about this many samples
Level set based eXtended finite element modelling of the response of fibrous networks under hygroscopic swelling
Materials like paper, consisting of a network of natural fibres, exposed to
variations in moisture, undergo changes in geometrical and mechanical
properties. This behaviour is particularly important for understanding the
hygro-mechanical response of sheets of paper in applications like digital
printing. A two-dimensional microstructural model of a fibrous network is
therefore developed to upscale the hygro-expansion of individual fibres,
through their interaction, to the resulting overall expansion of the network.
The fibres are modelled with rectangular shapes and are assumed to be perfectly
bonded where they overlap. For realistic networks the number of bonds is large
and the network is geometrically so complex that discretizing it by
conventional, geometry-conforming, finite elements is cumbersome. The
combination of a level-set and XFEM formalism enables the use of regular,
structured grids in order to model the complex microstructural geometry. In
this approach, the fibres are described implicitly by a level-set function. In
order to represent the fibre boundaries in the fibrous network, an XFEM
discretization is used together with a Heaviside enrichment function. Numerical
results demonstrate that the proposed approach successfully captures the
hygro-expansive properties of the network with fewer degrees of freedom
compared to classical FEM, preserving desired accuracy.Comment: 27 pages, 22 figures, 4 tables, J. Appl. Mech. June 19, 202
Assimilation of atmospheric methane products into the MACC-II system: From SCIAMACHY to TANSO and IASI
The Monitoring Atmospheric Composition and Climate Interim Implementation
(MACC-II) delayed-mode (DM) system has been producing an atmospheric methane
(CH4) analysis 6 months behind real time since June 2009. This analysis
used to rely on the assimilation of the CH4 product from the SCanning
Imaging Absorption spectroMeter for Atmospheric CHartographY (SCIAMACHY)
instrument onboard Envisat. Recently the Laboratoire de
Météorologie Dynamique (LMD) CH4 products from the Infrared
Atmospheric Sounding Interferometer (IASI) and the SRON Netherlands Institute
for Space Research CH4 products from the Thermal And Near-infrared Sensor
for carbon Observation (TANSO) were added to the DM system. With the loss of
Envisat in April 2012, the DM system now has to rely on the assimilation of
methane data from TANSO and IASI. This paper documents the impact of this
change in the observing system on the methane tropospheric analysis. It is
based on four experiments: one free run and three analyses from respectively
the assimilation of SCIAMACHY, TANSO and a combination of TANSO and IASI
CH4 products in the MACC-II system. The period between December 2010 and
April 2012 is studied. The SCIAMACHY experiment globally underestimates the
tropospheric methane by 35 part per billion (ppb) compared to the HIAPER
Pole-to-Pole Observations (HIPPO) data and by 28 ppb compared the Total
Carbon Column Observing Network (TCCON) data, while the free run presents an
underestimation of 5 ppb and 1 ppb against the same HIPPO and
TCCON data, respectively. The assimilated TANSO product changed in October
2011 from version v.1 to version v.2.0. The analysis of version v.1 globally
underestimates the tropospheric methane by 18 ppb compared to the
HIPPO data and by 15 ppb compared to the TCCON data. In contrast, the
analysis of version v.2.0 globally overestimates the column by 3 ppb.
When the high density IASI data are added in the tropical region between
30° N and 30° S, their impact is mainly positive but more
pronounced and effective when combined with version v.2.0 of the TANSO
products. The resulting analysis globally underestimates the column-averaged
dry-air mole fractions of methane (xCH4) just under 1 ppb on
average compared to the TCCON data, whereas in the tropics it overestimates
xCH4 by about 3 ppb. The random error is estimated to be less
than 7 ppb when compared to TCCON data
Adaptive density estimation for stationary processes
We propose an algorithm to estimate the common density of a stationary
process . We suppose that the process is either or
-mixing. We provide a model selection procedure based on a generalization
of Mallows' and we prove oracle inequalities for the selected estimator
under a few prior assumptions on the collection of models and on the mixing
coefficients. We prove that our estimator is adaptive over a class of Besov
spaces, namely, we prove that it achieves the same rates of convergence as in
the i.i.d framework
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