Genetic diversity and heterotic orientation of South Africa maize inbred lines towards tropical and temperate testers

Open Access Article; Published online: 20 Jul 2021An ecient hybrid breeding program denes and utilizes few heterotic groups. The objectives of this study were to determine genetic diversity and alignment of South Africa maize inbred lines collection towards tropical and temperate testers. Forty-two maize inbred lines were genotyped with 56110 single nucleotide polymorphism (SNP) DNA markers, using the Illumina MaizeSNP50 Bead chip. The 42 lines were crossed to two tropical and two temperate inbred line testers. The testcrosses were evaluated across seven environments, in South Africa, during 2014-2016. Genotypes and specic combining ability (SCA) effects of hybrids were signicantly different (P<0.05) for grain yield. There was a weak correlation between molecular genetic distances and both grain yield mean and specic combining ability effects of hybrids, indicating that productivity of maize inbred lines could not be reliably determined based on molecular genetic distances. The SCA data was capable of classifying these maize inbred lines into three heterotic groups with respect to both tropical and temperate testers. Only a few lines could not be grouped on the basis of SCA data. The study also indicated high level of diversity among the maize inbred lines, which was shown by both the dendogram and molecular genetic distances. The SNP marker data classied the inbred lines into 11 clusters that could be simplied into three major groups of normal maize endosperm and two groups of quality protein maize (QPM) endosperm types. However, the SNP data indicated that maize lines were more aligned towards tropical than temperate inbred testers. This information would be useful for simplifying heterotic classication of the lines with profound implications for breeding progress

Weed growth and labor demand under hand-hoe based reduced tillage in smallholder farmers’ fields in Zimbabwe

Conservation agriculture based on hand hoe dug planting basins has been widely promoted for the last decade or two in the smallholder farming sector of southern Africa targeting resource constrained households without access to draft power. In Zimbabwe planting basins are used by about one hundred thousand households but on small plots (<0.5 ha) although most are unable to adopt soil surface mulching and crop rotation due to competing uses for crop residues as livestock feed and poorly developed markets for other crops, respectively. We report on the effects of reduced tillage based on hand-hoe dug planting basins (PB) on weed growth (20 farms), and labor demand and returns to investment (50 farms) compared with animal-drawn mouldboard plough based conventional tillage (CONV) in maize (Zea mays L.) fields, across selected districts located in contrasting agro-ecological zones in Zimbabwe. Weed growth was assessed through a survey conducted at the end of the 2009/10 and 2010/11 cropping seasons. Labor demand and returns to investment were measured on 50 farms across five districts using direct observations during the 2011/12 cropping season. The survey showed that farmers on average weeded their PB plots 2.7 times per season compared to 1.7–1.9 times in CONV plots (P < 0.001), and timing was often delayed in the former. Reduced tillage plots had 17% (P < 0.001) more weed ground cover and 9% (P < 0.05) more weed dry matter compared with CONV plots in the 2009/10 season, and differences in 2010/11 were not significant. Weed growth was highest in semi-arid areas (natural regions III and IV) compared with wetter sub-humid areas (natural region II) and arid areas (natural region V). Farmers planted their PB plots 12–23 days earlier, weeding frequency was 42.1–58.9% higher in PB plots, compared with CONV. Labor demand was more than double under PB (84.7 man days ha−1, weeding 48.1 man days ha−1) compared to CONV (38.6 man days ha−1). However, returns to investment were 42.7% higher under PB (U$1.77) compared with CONV (U$1.24). Weed growth and labor demand remained high under PB tillage even after several years, interventions such as the use of alternative weed control methods need to be introduced to farmers to reduce labor demand and consequently increase its adoption both in terms of number of farmers and cultivated area in southern Africa

Exploring options for sustainable intensification in different farming system types of four Africa RISING countries

Sustainable intensification is proposed as a promising way to increase the productivity of agricultural systems while reducing pressure on ecosystems, safeguarding equitable relations among societal groups, and supporting the economic viability of households, enterprises, and communities. In sub-Saharan Africa, the identification and dissemination of options for sustainable intensification is hampered by the large diversity within and between farming systems, and their complexity arising from the interactions among different farm components and external factors. This study therefore uses an integrated farming systems approach to identify and assess context-specific improvements that can then be implemented and tested on-farm to foster experiential learning and facilitate adoption. We conducted a farming systems analysis for nine Africa RISING intervention sites across four countries, based on rapid and detailed farm characterizations, followed by model-supported diagnosis, and exploration of options for sustainable intensification. Farm diversity was described and analyzed by means of typologies and cross-site comparisons. Identified constraints varied depending on site and farming system type, but commonly included low input availability, climatic variability, poor soil fertility, sub-optimal livestock feeding, biotic stresses, and poor access to training and technical advice, all impairing farm productivity, returns to labor and capital inputs, income generation and food security. We investigated entry points that tackle the above constraints by exploring alternative farm configurations, technologies and practices for representative farms. By assessing potential impact of these changes on indicators beyond productivity, trade-offs were identified and assessed, for instance between profitability and household food self-sufficiency, and between nitrogen availability for crop uptake and increased nutrient losses. Taking a systems perspective during the entry point evaluation allowed differentiating potential effects on indicators at the field level versus the farm and household level. The exploration of options for specific farming system types now enables more targeted testing of promising innovations with farmers in the second project phase

Effect of Conservation Agriculture on Maize Yield in the Semi-Arid Areas of Zimbabwe

Globally, a range of agronomic factors have been reported to have an impact on the performance of conservation agriculture (CA) and often determine its performance in relation to conventional agriculture (CONV). To assess this performance in Zimbabwe, 48 CA experiments were conducted by the International Crops Research Institute for the Semi-Arid Tropics in the semi-arid areas of southern Zimbabwe from 2004 to 2010, to calculate the weighted mean difference (WMD) through meta-analytical methods. The two CA practices, planting basins (Basins) and ripper tillage (Ripper), were compared with CONV. It was hypothesised that CA results improved yield compared with CONV and that the effect of CA practices on yield is affected by soil type, rainfall amount and distribution and selected management practices, which included rates of inorganic fertilisers and manures and mulching. Basins were superior to CONV in 59% of the experiments and the overall effect was significant (p < 0.001). The effect of Ripper was non-significant. The hypothesis that CA practices result in improved maize grain yield over CONV was accepted for Basins. The WMD for experiments conducted on sandy soils was 0.365 t ha−1 for Basins and 0.184 t ha−1 for Ripper, and in both cases was significant (p < 0.05). For clay soils, only the WMD for Basins was significant. A higher rainfall regime (500–830 mm) resulted in a lower WMD for Basins (0.095 t ha−1) and Ripper (0.105 t ha−1) compared with 0.151 t ha−1 for Basins and 0.110 t ha−1 for Ripper under lower rainfall (320–500 mm). The overall effect of Basins under the higher rainfall regime was not significant. There was better yield performance for Basins when the rainfall was well distributed; the reverse was noted for the Ripper. The application of 10–30 kg ha−1 of N (micro-dose range) resulted in a higher WMD for Basins than zero N application. Without N application, the WMD of Basins was not significant. For zero manure application in Basins, the WMD was 0.043 t ha−1 compared with 0.159 t ha−1 when manure was applied. The application of mulch depressed the WMD in Basins by 44% and Ripper by 89%. The hypothesis that yield performance under CA is influenced by soil type, rainfall amount and distribution, inorganic fertiliser and manure application was accepted

Immunization with lytic polysaccharide monooxygenase CbpD induces protective immunity against Pseudomonas aeruginosa pneumonia

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Critical reflection on knowledge and narratives of conservation agriculture

In the context of contemporary concerns about climate change and food security, Conservation Agriculture (CA) has emerged as a well-supported and central component of the agricultural sector development strategy across sub-Saharan Africa, including in Zambia, which is the focus of this paper. A variety of narratives about the benefits of CA over conventional agricultural systems underpin endeavours towards ‘scaling up’ CA and increasing rates of adoption amongst smallholder farmers nationwide. However, there is a knowledge politics underlying the translation of a weak evidence base around CA into persuasive narratives and financial and political support. In this paper, we trace the evolution of five narratives around CA in Zambia in relation to changing political agendas and the involvement of new public and private sector actors, and review the development of evidence bases and knowledge that support and challenge each of these narratives. We discuss the potential to open up space within this knowledge politics to alternative narratives and the contestation of the pervasive CA scaling up agenda. Critical reflection is essential to ensure that national and local evidence is more effectively used to guide national climate and agricultural policy developments and international donor initiatives

Approaches in biotechnological applications of natural polymers

Natural polymers, such as gums and mucilage, are biocompatible, cheap, easily available and non-toxic materials of native origin. These polymers are increasingly preferred over synthetic materials for industrial applications due to their intrinsic properties, as well as they are considered alternative sources of raw materials since they present characteristics of sustainability, biodegradability and biosafety. As definition, gums and mucilages are polysaccharides or complex carbohydrates consisting of one or more monosaccharides or their derivatives linked in bewildering variety of linkages and structures. Natural gums are considered polysaccharides naturally occurring in varieties of plant seeds and exudates, tree or shrub exudates, seaweed extracts, fungi, bacteria, and animal sources. Water-soluble gums, also known as hydrocolloids, are considered exudates and are pathological products; therefore, they do not form a part of cell wall. On the other hand, mucilages are part of cell and physiological products. It is important to highlight that gums represent the largest amounts of polymer materials derived from plants. Gums have enormously large and broad applications in both food and non-food industries, being commonly used as thickening, binding, emulsifying, suspending, stabilizing agents and matrices for drug release in pharmaceutical and cosmetic industries. In the food industry, their gelling properties and the ability to mold edible films and coatings are extensively studied. The use of gums depends on the intrinsic properties that they provide, often at costs below those of synthetic polymers. For upgrading the value of gums, they are being processed into various forms, including the most recent nanomaterials, for various biotechnological applications. Thus, the main natural polymers including galactomannans, cellulose, chitin, agar, carrageenan, alginate, cashew gum, pectin and starch, in addition to the current researches about them are reviewed in this article.. }To the Conselho Nacional de Desenvolvimento Cientfíico e Tecnológico (CNPq) for fellowships (LCBBC and MGCC) and the Coordenação de Aperfeiçoamento de Pessoal de Nvíel Superior (CAPES) (PBSA). This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit, the Project RECI/BBB-EBI/0179/2012 (FCOMP-01-0124-FEDER-027462) and COMPETE 2020 (POCI-01-0145-FEDER-006684) (JAT)

Genetic trends in CIMMYT’s tropical maize breeding pipelines

Fostering a culture of continuous improvement through regular monitoring of genetic trends in breeding pipelines is essential to improve efficiency and increase accountability. This is the first global study to estimate genetic trends across the International Maize and Wheat Improvement Center (CIMMYT) tropical maize breeding pipelines in eastern and southern Africa (ESA), South Asia, and Latin America over the past decade. Data from a total of 4152 advanced breeding trials and 34,813 entries, conducted at 1331 locations in 28 countries globally, were used for this study. Genetic trends for grain yield reached up to 138 kg ha−1 yr−1 in ESA, 118 kg ha−1 yr−1 South Asia and 143 kg ha−1 yr−1 in Latin America. Genetic trend was, in part, related to the extent of deployment of new breeding tools in each pipeline, strength of an extensive phenotyping network, and funding stability. Over the past decade, CIMMYT’s breeding pipelines have significantly evolved, incorporating new tools/technologies to increase selection accuracy and intensity, while reducing cycle time. The first pipeline, Eastern Africa Product Profile 1a (EA-PP1a), to implement marker-assisted forward-breeding for resistance to key diseases, coupled with rapid-cycle genomic selection for drought, recorded a genetic trend of 2.46% per year highlighting the potential for deploying new tools/technologies to increase genetic gain