Musicalidades dialéticas: transmetodologia para uma ciência sonora

The article proposes a dialogue about the epistemological conception of the method called Dialectical Musicalities, which has as its guiding principles theories of the Mattelart strand, read and expanded by Alberto Efendy Maldonado, through the concept of transmetology. The author presents four guiding principles and how they are carried out in investigative practices through a singular and applied methodological design.O artigo propõe um diálogo sobre a concepção epistemológica do método chamado musicalidades dialéticas, que tem como princípios norteadores teorias da vertente Mattelart, lidas e ampliadas por Alberto Efendy Maldonado, através do conceito de transmetodologia. O autor apresenta quatro princípios norteadores e como se realizam em práticas investigativas através de desenho metodológico singular e aplicado

Riscos na análise de crédito em uma cooperativa localizada no sul do estado de Santa Catarina

Trabalho de Conclusão de Curso, apresentado para obtenção do grau de bacharel no curso de Ciências Contábeis da Universidade do Extremo Sul Catarinense, UNESC.O objetivo geral deste trabalho consiste em uma análise sobre o risco de crédito em uma cooperativa no sul do Estado de Santa Catarina, buscando as ferramentas necessárias para evitar possíveis perdas na concessão de crédito, destacar a importância e demonstrar como a mesma pode ser utilizada, posto que a decisão para concessão de crédito deve ser objeto de cautela por parte dos administradores. A construção de bases para a decisão, principalmente sobre condições de risco, deve ser constituída por meio de ferramentas que tragam o resultado desejado. As empresas devem estar cientes e fazer uso de preceitos básicos na concessão do crédito, considerando as características e o perfil do cliente através de um cadastro adequado e que proporcione informações seguras, amplas e autênticas. Será verificado o método utilizado por uma Cooperativa do Sul de Santa Catarina, para evitar possíveis riscos quando da concessão de crédito, onde será apresentada uma proposta para que a cooperativa em estudo adote medidas proativas que acarretem menores riscos e a implantação de uma política de crédito que atenda às necessidades da organização de forma que não prejudique seu ciclo financeiro. O presente estudo demonstra relevância, pois permite o aprimoramento do conhecimento acerca desta área da contabilidade, possibilita o conhecimento sobre a política de crédito, o processo de concessão e análise de crédito adotados pelas empresas e pela cooperativa estudada, podendo inclusive ser de grande valia na vida profissional pois agrega informações que poderão ser aplicadas no cotidiano de qualquer empresa exposta a riscos que possam trazer prejuízos

Comparando edições de ‘Senhora’, de José de Alencar: um exercício de Crítica Textual

De posse da edição crítica de Senhora, de José de Alencar, e de outras nove edições provenientes de diversas fontes - internet, biblioteca da Faculdade de Letras da UFRJ e de acervos pessoais -, foi feita uma análise comparativa apoiada nos conceitos expostos no livro Introdução à Crítica Textual, de César Nardelli Cambraia (2005). O trabalho, desenvolvido para a avaliação final da disciplina Introdução à Filologia do curso de Letras da UFRJ, em 2016.2, visou a tentar encontrar as diferenças entre as edições disponíveis para estudo, além de também tentar agrupá-las de acordo com suas possíveis edições de origem. Para tal, foi seguida a metodologia de Bassetto (2001): recensio, collatio codicum e estemática. Ainda foram levados em conta os elementos da crítica histórico-literária (BASSETTO, 2001): circunstância, unidade e integridade e linguagem do texto. Foram então observados erros de cópia em todos os volumes, erros estes que geram consequências gramaticais, semânticas, estilísticas e até mesmo históricas. Assim, o trabalho em questão também propõe discutir esses efeitos no momento de leitura e análise da obra

Conjugation of different immunogenic enterococcal vaccine target antigens leads to extended strain coverage

[Abstract] Enterococci have emerged as important nosocomial pathogens due to their resistance to the most commonly used antibiotics. Alternative treatments or prevention options are aimed at polysaccharides and surface-related proteins that play important roles in pathogenesis. Previously, we have shown that 2 Enterococcus faecium proteins, the secreted antigen A and the peptidyl-prolyl cis-trans isomerase, as well as the Enterococcus faecalis polysaccharide diheteroglycan, are able to induce opsonic and cross-protective antibodies. Here, we evaluate the use of glycoconjugates consisting of these proteins and an enterococcal polysaccharide to develop a vaccine with broader strain coverage. Diheteroglycan was conjugated to these 2 enterococcal proteins. Rabbit sera raised against these glycoconjugates showed Immunoglobulin G titers against the corresponding conjugate, as well as against the respective protein and carbohydrate antigens. Effective opsonophagocytic killing for the 2 sera was observed against different E. faecalis and E. faecium strains. Enzyme-linked immunosorbent assays against whole bacterial cells showed immune recognition of 22 enterococcal strains by the sera. Moreover, the sera conferred protection against E. faecalis and E. faecium strains in a mouse infection model. Our results suggest that these glycoconjugates are promising candidates for vaccine formulations with a broader coverage against these nosocomial pathogens and that the evaluated proteins are potential carrier proteins

Prehospital neurological deterioration in stroke

BACKGROUND AND PURPOSE: Patients with stroke can experience neurological deterioration in the prehospital setting. We evaluated patients with stroke to determine factors associated with prehospital neurological deterioration (PND). METHODS: Among the Greater Cincinnati/Northern Kentucky region (population ~1.3 million), we screened all 15 local hospitals' admissions from 2010 for acute stroke and included patients aged ≥20. The GCS was compared between emergency medical services (EMS) arrival and hospital arrival, with decrease ≥2 points considered PND. Data obtained retrospectively included demographics, medical history and medication use, stroke subtype (eg, ischaemic stroke (IS), intracerebral haemorrhage (ICH), subarachnoid haemorrhage (SAH)) and IS subtype (eg, small vessel, large vessel, cardioembolic), seizure at onset, time intervals between symptom onset, EMS arrival and hospital arrival, EMS level of training, and blood pressure and serum glucose on EMS arrival. RESULTS: Of 2708 total patients who had a stroke, 1092 patients (median (IQR) age 74 (61-83) years; 56% women; 21% black) were analysed. PND occurred in 129 cases (12%), including 9% of IS, 24% of ICH and 16% of SAH. In multivariable analysis, black race, atrial fibrillation, haemorrhagic subtype and ALS level of transport were associated with PND. CONCLUSION: Haemorrhage and atrial fibrillation is associated with PND in stroke, and further investigation is needed to establish whether PND can be predicted. Further studies are also needed to assess whether preferential transport of patients with deterioration to hospitals equipped with higher levels of care is beneficial, identify why race is associated with deterioration and to test therapies targeting PND

Análisis de la distribución de microdureza, contenido mineral y orgánico de dientes humanos, bovinos y ovinos

Objetive. Human teeth have been commonly used for in vitro and in situ studies. Cu­rrently, other animals’ teeth have been purposed for dental research to overcome human teeth’ problematic availability. This study aimed to investigate the enamel and dentin from human, bovine, and ovine teeth concerning the microhardness, organic, and inorganic contents via micro-Raman spectroscopy. Methods. Human, bovine, and ovine teeth were divided according to their type and age into seven groups: Ovine; Bovine-12 months; Bovine-24 months; Bovine-36 months; Bovine-48 months; Bovine-+60 months; Human (control). The enamel’s microhardness (superficial and deep) and dentin (superficial, middle, and deep) were analyzed. The calcium/phosphate ratio and amide contents were determined by micro-Raman spectroscopy. Results. Overall, the microhardness of human enamel was superior to the other species. Dentin’s microhardness was similar among groups. Ovine group showed lower values of calcium/phosphate ratio than human. Amide content was similar between bovine and human. The microhardness and calcium/phosphate ratio of enamel and dentin, respectively, decreased as the age of bovine teeth increased. Conclusions. Researchers must be aware and take into consideration the differences of ovine and bovine enamel compared to human enamel. Other alternatives that are more similar to the microhardness of human enamel should be sought. Bovine teeth of 12 and 24 months are suitable substitutes for dentin of human teeth. Researchers must also be aware of the age of the animals and specify it in the studies.Objetivo. Los dientes humanos se han utilizado comúnmente para estudios in vitro e in situ. Actualmente, los dientes de otros animales se han destinado a la investigación dental para superar la disponibilidad problemática de los dientes humanos. Este estudio tuvo como objetivo investigar el esmalte y la dentina de los dientes humanos, bovinos y ovinos en relación con la microdureza y los contenidos orgánicos e inorgánicos a través de la espectroscopia micro-Raman. Métodos. Los dientes humanos, bovinos y ovinos se dividieron según su tipo y edad en siete grupos: Ovinos; Bovino-12 meses; Bovino-24 meses; Bovino-36 meses; Bovino-48 meses; Bovino-+60 meses; Humano (control). Se analizó la microdureza del esmalte (superficial y profunda) y de la dentina (superficial, media y profunda). La relación calcio/fosfato y los contenidos de amida se determinaron mediante espectroscopía micro-Raman. Resultados. En general, la microdureza del esmalte humano fue superior a la de otras especies. La microdureza de la dentina fue similar entre los grupos. El grupo ovino mostró valores más bajos de la relación calcio/fosfato que el humano. El contenido de amida fue similar entre bovinos y humanos. La microdureza y la relación calcio/fosfato del esmalte y la dentina, respectivamente, disminuyeron a medida que aumentaba la edad de los dientes bovinos. Conclusiones. El esmalte de los dientes ovinos y bovinos no es un sustituto adecuado del de los dientes humanos. Se deben buscar otras alternativas que sean similares a la microdureza del esmalte humano. Sin embargo, los dientes bovinos de 12 y 24 meses son sustitutos adecuados de la dentina de los dientes humanos. Los investigadores deben conocer la edad de los animales y especificarla en los estudios

Effect of silver nanoparticles on the physicochemical and antimicrobial properties of an orthodontic adhesive

Ortodontiv treatment with fixed brackets plays a mayor role on the formation of white spot lesions. Objective:Tadhesive and evaluate its physicochemical and antimicrobial properties. Material and Methods: Silver nanoparticle solutions were added to a commercial adhesive in different concentrations (w/w): 0%, 0.11%, 0.18%, and 0.33%. Shear bond strength (SBS) test was performed after bonding metal brackets to enamel. Raman spectroscopy was used to analyze in situ the degree of conversion (DC) of the adhesive layer. The surface free energy (SFE) was evaluated after the measurement of contact angles. Growth inhibition of Streptococcus mutans in liquid and solid media was determined by colony-forming unit count and inhibition halo, respectively. One-way ANOVA was performed for SBS, DC, SFE, and growth inhibition. Results: The incorporation of AgNP solution decreased the SBS (p<0.001) and DC in situ (p<0.001) values. SFE decreased after addition of 0.18% and 0.33% AgNP. Growth inhibition of S. mutans in liquid media was obtained after silver addition (p<0.05). Conclusions: The addition of AgNP solutions to Transbond™ XT adhesive primer inhibited S. mutans growth. SBS, DC, and SFE values decreased after incorporation up to 0.33% AgNP solution without compromising the chemical and physical properties of the adhesive

Main degradation products of dabigatran etexilate evaluated by LC-UV and LC-ESI-MS, degradation kinetics and in vitro cytotoxicity studies

The present study reports the stability profile of an antithrombotic drug: dabigatran etexilate (DAB). The drug was subjected to thermal degradation at 60 °C and products formed were investigated by liquid chromatography-UV (LC-UV) and liquid chromatography-mass spectrometry (LC-ESI-MS). Chromatographic separation of the degradation products was performed on a GL Sciences Inc. Inertsil ODS-2 column (250 mm × 4.6 mm i.d., with a particle size of 5 µm and pore size of 110 Å) with mobile phase consisting of acetonitrile and ammonium acetate buffer (pH 5.5; 10 mmol L−1 ) (65:35, v/v) pumped at 1.0 mL min−1 flow rate. Column temperature was set at 30 °C and detection at 225 nm using a UV detector. LC-UV method previously validated was extended to LC-ESI-MS for the characterization of the degradation products (DP-01 and DP-02) formed, without complicated isolation or purification processes, based on retention times and confirmation of molecular weight. Degradation kinetics of DAB was also evaluated and could be described as a first-order process (R2 = 0.9900). Furthermore, no evidence of cytotoxicity in human mononuclear cells was observed for DAB degraded samples

Effect of silver nanoparticles on the physicochemical and antimicrobial properties of an orthodontic adhesive

Orthodontic treatment with fixed brackets plays a major role on the formation of white spot lesions. Objective This study aimed to incorporate silver nanoparticle solutions (AgNP) in an orthodontic adhesive and evaluate its physicochemical and antimicrobial properties. Material and Methods Silver nanoparticle solutions were added to a commercial adhesive in different concentrations (w/w): 0%, 0.11%, 0.18%, and 0.33%. Shear bond strength (SBS) test was performed after bonding metal brackets to enamel. Raman spectroscopy was used to analyze in situ the degree of conversion (DC) of the adhesive layer. The surface free energy (SFE) was evaluated after the measurement of contact angles. Growth inhibition of Streptococcus mutans in liquid and solid media was determined by colony-forming unit count and inhibition halo, respectively. One-way ANOVA was performed for SBS, DC, SFE, and growth inhibition. Results The incorporation of AgNP solution decreased the SBS (