Circulating Exosomal Mir21 And Mir320 In Obstructive Sleep Apnea

Rational. Epidemiological studies indicate that there may be an association between obstructive sleep apnea (OSA) and cardiovascular and metabolic diseases. Some pro-inflammatory miRs (miR-21, miR320) critical for the immune response or hypoxia are often overexpressed in cancers and atherosclerosis. Aim. To examine the expression of miR-21& miR320 in circulating exosomes from patients with OSA. Methods: From a Sleep Unit and in the frame of a long-term longitudinal cohort study we selected 65 non-smokers OSA patients (apnea-hypopnea index -AHI- 30 events/ti) and 26 age, gender and BMI-matched controls (AHI 0.85 mm. Plasma-derived exosomes were isolated by precipitation using miRCURY, , , Exosome Isolation Kit. Exosomes were characterized by transmission electron microscopy, dynamic light scattering assay and Western Blot analysis using CD63 and HSP70. Exosome total RNA was obtained using miRCURY"* RNA isolation kit. miR-21 -5p and miR-320-3p were analysed by real time quantitative PCR (RT-qPCR) using miRCURY LNA~ technology..

Myosporidium merluccius n. g., n. sp. Infecting Muscle of Commercial Hake (Merluccius sp.) from Fisheries near Namibia

A new species of Microsporidia classified to a new genus was observed in the trunk muscle of commercial hake (Merluccius capensis/paradoxus complex) from Namibian fisheries. Macroscopic examination revealed thin and dark filaments inserted among muscle fibers. Inside the filaments were many sporophorous vesicles with about 30–50 spores per vesicle. The shape of the spore was pyriform and the extruded polar filament was of moderate length (up to 4.29 mm, n512). This new species of Microsporidia is described using macrophotography, microphotography, staining, and transmission electron microscopy (TEM), as well as molecular methods. Its 16S rRNA was found to be similar to that of Microsporidium prosopium Kent et al., 1999, while both sequences were quite different from 16S rRNA sequences known for other Microsporidia. Nevertheless, this new species is separated morphologically from M. prosopium by the presence of 11–12 anisofilar coils and the formation of the xenoma at the site of infection. Type specie

Evaluation of Fluorescent Confocal Microscopy for Intraoperative Analysis of Prostate Biopsy Cores

The use of fluorescent confocal microscopy allows fast and easy preparation of biopsy cores for intraoperative analysis. We observed strong concordance with traditional analysis in our study. The role of this technique is yet to be established

Experimental verification of memristor-based material implication NAND operation

Memristors are being considered as promising devices for highly dense memory systems as well as the potential basis of new computational paradigms. In this scenario, and in relation with data processing, one of the more specific and differential logic functions is the material implication logic also named as IMPLY logic. Many papers have been published in this framework but few of them are related with experimental works using real memristor devices. In the paper authors show the verification of the IMPLY function by using Ni/HfO2/Si manufactured devices and laboratory measurements. The proper behavior of the IMPLY structure (2 memristors) has been shown. The paper also verifies the proper operation of a two-step IMPLY-based NAND gate implementation, showing the electrical behavior of the circuit in a cycling operation. A new procedure to implement a NAND gate that requires only one step is experimentally shown as well

The infrared Hourglass cluster in M8

A detailed study of the Hourglass Nebula in the M8 star forming region is presented. The study is mainly based on recent subarcsec-resolution JHKs images taken at Las Campanas Observatory and complemented with archival HST images and longslit spectroscopy retrieved from the ESO Archive Facility. Using the new numerical code CHORIZOS, we estimate the distance to the earliest stars in the region to be 1.25 kpc. Infrared photometry of all the sources detected in the field is given. From analysis of the JHKs colour-colour diagrams, we find that an important fraction of these sources exhibit significant infrared excess. These objects are candidates to be low- and intermediate-mass pre-main sequence stars. Based on HST observations, the spatial distribution of gas, dust and stars in the region is analyzed. The morphological analysis of these images also reveals a rich variety of structures related to star formation (proplyds, jets, bow shocks), similar to those observed in M16 and M42, along with the detection of the first four Herbig-Haro objects in the region. Furthermore, a longslit spectrum obtained with NTT confirms the identification of one of them (HH 870) in the core of the Hourglass nebula, providing the first direct evidence of active star formation by accretion in M8.Comment: 22 pages, 15 figures, 3 tables, submitted to MNRAS. A preprint with high-resolution figures is available at http://www.dfuls.cl/~rbarba/arias_hourglass.pd

From favorable atomic configurations to supershell structures: a new interpretation of conductance histograms

Title: From favorable atomic configurations to supershell structures: a new interpretation of conductance histograms Authors: A. Hasmy (IVIC), E. Medina (IVIC), P.A. Serena (CSIC,IVIC) Comments: 7 pages, 3 figures, cond-mat.anwar.10825 Subj-class: Soft Condensed MatterComment: 7 pages, 3 figuresSubject: fput HMS.tex HMS-FIG1.ps HMS-FIG2.ps HMS-FIG3.p

Stiff monatomic gold wires with a spinning zigzag geometry

Using first principles density functional calculations, gold monatomic wires are found to exhibit a zigzag shape which remains under tension, becoming linear just before breaking. At room temperature they are found to spin, what explains the extremely long apparent interatomic distances shown by electron microscopy.The zigzag structure is stable if the tension is relieved, the wire holding its chainlike shape even as a free-standing cluster. This unexpected metallic-wire stiffness stems from the transverse quantization in the wire, as shown in a simple free electron model.Comment: 4 pages, latex, 5 figures, submitted to PR

Mantle-cell lymphoma genotypes identified with CGH to BAC microarrays define a leukemic subgroup of disease and predict patient outcome

To identify recurrent genomic changes in mantle cell lymphoma (MCL), we used high-resolution comparative genomic hybridization (CGH) to bacterial artificial chromosome (BAC) microarrays in 68 patients and 9 MCL-derived cell lines. Array CGH defined an MCL genomic signature distinct from other B-cell lymphomas, including deletions of 1p21 and 11q22.3-ATM gene with coincident 10p12-BMI1 gene amplification and 10p14 deletion, along with a previously unidentified loss within 9q21-q22. Specific genomic alterations were associated with different subgroups of disease. Notably, 11 patients with leukemic MCL showed a different genomic profile than nodal cases, including 8p21.3 deletion at tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) receptor gene cluster (55% versus 19%; P = .01) and gain of 8q24.1 at MYC locus (46% versus 14%; P = .015). Additionally, leukemic MCL exhibited frequent IGVH mutation (64% versus 21%; P = .009) with preferential VH4-39 use (36% versus 4%; P = .005) and followed a more indolent clinical course. Blastoid variants, increased number of genomic gains, and deletions of P16/INK4a and TP53 genes correlated with poorer outcomes, while 1p21 loss was associated with prolonged survival (P = .02). In multivariate analysis, deletion of 9q21-q22 was the strongest predictor for inferior survival (hazard ratio [HR], 6; confidence interval [CI], 2.3 to 15.7). Our study highlights the genomic profile as a predictor for clinical outcome and suggests that "genome scanning" of chromosomes 1p21, 9q21-q22, 9p21.3-P16/INK4a, and 17p13.1-TP53 may be clinically useful in MCL