Characterization of the major nuclear localization signal of the Borna disease virus phosphoprotein

Borna disease virus (BDV) replicates and transcribes its negative-sense RNA genome in the nucleus. The BDV phosphoprotein (P) is localized in the nucleus of infected cells and cells transfected with P expression constructs. To identify the nuclear localization signal (NLS) of P, COS- 7 cells were transfected with wild-type or mutant forms of P fused with green fluorescent protein (GFP). Whereas GFP alone was exclusively cytoplasmic, P or P-GFP were nuclear. Analysis of carboxy- and amino- terminal truncation mutants of P indicated that amino acids (aa) 20-37 are sufficient to promote efficient nuclear accumulation of the fusion protein. Residual nuclear import of GFP was observed with portions of P including aa 33-134 or aa 134-201, suggesting the presence of additional NLS motifs. The major NLS of P appears to be bipartite. It consists of two basic aa domains, R22RER25 and R30PRKIPR36, separated by four non-basic aa, S26GSP29

Authentic Borna disease virus transcripts are spliced less efficiently than cDNA-derived viral RNAs

Borna disease virus (BDV) is a non-segmented, negative-strand RNA virus that replicates and transcribes its genome in the nucleus of infected cells. It uses the cellular splicing machinery to generate a set of alternatively spliced mRNAs from the 2.8 and 7.1 kb primary transcripts, each harbouring two introns. To determine whether splicing of these transcripts is regulated by viral factors, the extent of splicing was studied in infected cells and COS-7 cells transiently transfected with plasmids encoding the 2.8 kb RNA of BDV. Unspliced RNA was found to be the most abundant RNA species in infected cells, whereas viral transcripts lacking both introns were only found in minute amounts. In sharp contrast, plasmid-derived 2.8 kb RNA was predominantly intron 1-spliced and double-spliced. Co-expression of the BDV proteins P, N and X did not influence splicing of plasmid-expressed 2.8 kb RNA. Furthermore, the splicing pattern did not change when the 2.8 kb RNA was expressed in BDV-infected cells. Based on these results we speculate that splicing of authentic BDV transcripts is tightly linked to transcription by the viral polymerase

Strong and Electromagnetic Decays of the Baryon Decuplet

We discuss the strong and radiative decays of the decuplet of baryon resonances to the baryon octet in chiral perturbation theory. We comment on the implications for the polarisability of the nucleon.Comment: 16 pages + 5 figures (.ps files) appended to file. Uses tables.tex and harvmac.te

Quasi-nuclear and quark model baryonium: historical survey

We review ideas and speculations concerning possible bound states or resonances coupled to the nucleon-antinucleon channel.Comment: 7 pages, no figure, Latex with espcrc2.sty, Talk at QCD99, Montpellier, France, July 1999, to appear in the Proceedings, ed. S. Nariso

Two electron entanglement enhancement by an inelastic scattering process

In order to assess inelastic effects on two fermion entanglement production, we address an exactly solvable two-particle scattering problem where the target is an excitable scatterer. Useful entanglement, as measured by the two particle concurrence, is obtained from post-selection of oppositely scattered particle states. The $S$ matrix formalism is generalized in order to address non-unitary evolution in the propagating channels. We find the striking result that inelasticity can actually increase concurrence as compared to the elastic case by increasing the uncertainty of the single particle subspace. Concurrence zeros are controlled by either single particle resonance energies or total reflection conditions that ascertain precisely one of the electron states. Concurrence minima also occur and are controlled by entangled resonance situations were the electron becomes entangled with the scatterer, and thus does not give up full information of its state. In this model, exciting the scatterer can never fully destroy phase coherence due to an intrinsic limit to the probability of inelastic events.Comment: 8 pages, to appear in Phys. Rev

Light and heavy multiquark spectroscopy

The dynamics of multiquark binding is revisited in the light of the recent experimental results. It is emphasized that some configurations mixing light and heavy flavours are among the most favourable for stable or metastable multiquarks. The nuclear-physics type of approach predicting the so-called hadron-hadron molecules is compared to direct studies in terms of quark interaction.Comment: Invited talk at QCD05, 12th International QCD Conference, July 4-9, 2005 - Montpellier (France), to appear in the proceedings edited by S. Nariso

Elastic and large t rapidity gap vector meson production in ultraperipheral proton-ion collisions

We evaluate the cross sections for the production of vector mesons in exclusive ultraperipheral proton-ion collisions at LHC. We find that the rates are high enough to study the energy and momentum transfer dependence of vector mesons - rho,phi, J/psi, Upsilon photoproduction in gamma p scattering in a wide energy range extending the measurements which were performed at HERA, providing new information about interplay of soft and hard physics in diffraction. Also, we calculate the contributions to the vector meson yield due to production of vector mesons off nuclear target by photons emitted by proton. We find, that least in the case of Upsilon production it is feasible to observe simultaneously both these processes. Such measurements would increase the precision with which the A-dependence of exclusive onium production can be determined. This would also enable one to estimate the amount of nuclear gluon shadowing of generalized gluon distributions at much smaller x than that is possible in AA collisions and to measure the cross sections for photoproduction processes in a significantly wider energy range than that achieved in experiments with fixed nuclear targets. We also present the cross section for vector meson production in pA collisions at RHIC. In addition, we consider production of vector mesons off protons with large rapidity gaps and large t. These processes probe small x dynamics of the elastic interaction of small dipoles at high energies and large but finite t, that is in the kinematics where DGLAP evolution is strongly suppressed. We estimate that this process could be studied at LHC up to W ~1 TeV with detectors which will be available at LHC.Comment: 21 pages, 9 figures, a reference to experimental data is adde

NLO differential distributions of massive lepton-pair production in longitudinally polarized proton-proton collisions

We present the full next-to-leading order (NLO) corrected inclusive cross section $d^3\Delta \sigma/dQ^2/dy/dp_T$ for massive lepton pair production in longitudinally polarized proton-proton collisions $p + p\to l^+l^- + 'X'$. Here $'X'$ denotes any inclusive hadronic state and Q represents the invariant mass of the lepton pair which has transverse momentum p_T and rapidity y. All QCD partonic subprocesses have been included provided the lepton pair is created by a virtual photon, which is a valid approximation for $Q<50{\rm GeV}$. Like in unpolarized proton-proton scattering the dominant subprocess is given by $q(\bar q) + g \to \gamma^* + 'X'$ so that massive lepton pair production provides us with an excellent method to measure the spin density of the gluon. Our calculations are carried out using the method of n-dimensional regularization by making a special choice for the $\gamma_5$-matrix. Like in the case of many other prescriptions evanescent counter terms appear. They are determined by computing the NLO coefficient functions for $d\Delta\sigma/dQ^2$ and the polarized cross section for Higgs production using both n-dimensional regularization and a four dimensional regularization technique in which the $\gamma_5$-matrix is uniquely defined. Our calculations reveal that the non-singlet polarized coefficient function equals the unpolarized one up to a minus sign. We give predictions for double longitudinal spin asymmetry measurements at the RHIC.Comment: 56 pages, LaTeX, 20 postscript figures. We have changed the discussion of the various regularization schemes in the penultimate paragraph of section 1 and in the text between Eq. (2.17) and Eq. (2.18). Furthermore a more careful comparison between our results and those in refs. [13],[14] for the quark-anti-quark channel reveals that we have full agreement. Further some misprints like the ones occuring in Eq. (2.61) and table 1 in section 4 are correcte

Identification of Neutral B Mesons Using Correlated Hadrons

The identification of the flavor of a neutral $B$ meson can make use of hadrons produced nearby in phase space. Examples include the decay of ``$B^{**}$'' resonances or the production of hadrons as a result of the fragmentation process. Some aspects of this method are discussed, including time-dependent effects in neutral $B$ decays to flavor states, to eigenstates of CP and to other states, and the effects of possible coherence between $B^0$ and $\overline{B}^0$ in the initial state. We study the behavior of the leading hadrons in $b$-quark jets and the expected properties of $B^{**}$ resonances. These are extrapolated from the corresponding $D^{**}$ resonances, of whose properties we suggest further studies.Comment: To be submitted to Phys. Rev. D. 26 pages, LaTeX, figures not included (available upon request). Technion-PH-93-32 / EFI 93-4

Etiology of severe childhood pneumonia in the Gambia, West Africa, determined by conventional and molecular microbiological analyses of lung and pleural aspirate samples.

Molecular analyses of lung aspirates from Gambian children with severe pneumonia detected pathogens more frequently than did culture and showed a predominance of bacteria, principally Streptococcus pneumoniae, >75% being of serotypes covered by current pneumococcal conjugate vaccines. Multiple pathogens were detected frequently, notably Haemophilus influenzae (mostly nontypeable) together with S. pneumoniae