Fine-Structure FeII* Emission and Resonant MgII Emission in z = 1 Star-Forming Galaxies

We present a study of the prevalence, strength, and kinematics of ultraviolet FeII and MgII emission lines in 212 star-forming galaxies at z = 1 selected from the DEEP2 survey. We find FeII* emission in composite spectra assembled on the basis of different galaxy properties, indicating that FeII* emission is prevalent at z = 1. In these composites, FeII* emission is observed at roughly the systemic velocity. At z = 1, we find that the strength of FeII* emission is most strongly modulated by dust attenuation, and is additionally correlated with redshift, star-formation rate, and [OII] equivalent width, such that systems at higher redshifts with lower dust levels, lower star-formation rates, and larger [OII] equivalent widths show stronger FeII* emission. We detect MgII emission in at least 15% of the individual spectra and we find that objects showing stronger MgII emission have higher specific star-formation rates, smaller [OII] linewidths, larger [OII] equivalent widths, lower dust attenuations, and lower stellar masses than the sample as a whole. MgII emission strength exhibits the strongest correlation with specific star-formation rate, although we find evidence that dust attenuation and stellar mass also play roles in the regulation of MgII emission. Future integral field unit observations of the spatial extent of FeII* and MgII emission in galaxies with high specific star-formation rates, low dust attenuations, and low stellar masses will be important for probing the morphology of circumgalactic gas.Comment: 29 pages, 22 figures, 2 tables; accepted to Ap

Mesenchymal progenitor cell markers in human articular cartilage: normal distribution and changes in osteoarthritis

INTRODUCTION: Recent findings suggest that articular cartilage contains mesenchymal progenitor cells. The aim of this study was to examine the distribution of stem cell markers (Notch-1, Stro-1 and VCAM-1) and of molecules that modulate progenitor differentiation (Notch-1 and Sox9) in normal adult human articular cartilage and in osteoarthritis (OA) cartilage. METHODS: Expression of the markers was analyzed by immunohistochemistry (IHC) and flow cytometry. Hoechst 33342 dye was used to identify and sort the cartilage side population (SP). Multilineage differentiation assays including chondrogenesis, osteogenesis and adipogenesis were performed on SP and non-SP (NSP) cells. RESULTS: A surprisingly high number (>45%) of cells were positive for Notch-1, Stro-1 and VCAM-1 throughout normal cartilage. Expression of these markers was higher in the superficial zone (SZ) of normal cartilage as compared to the middle zone (MZ) and deep zone (DZ). Non-fibrillated OA cartilage SZ showed reduced Notch-1 and Sox9 staining frequency, while Notch-1, Stro-1 and VCAM-1 positive cells were increased in the MZ. Most cells in OA clusters were positive for each molecule tested. The frequency of SP cells in cartilage was 0.14 +/- 0.05% and no difference was found between normal and OA. SP cells displayed chondrogenic and osteogenic but not adipogenic differentiation potential. CONCLUSIONS: These results show a surprisingly high number of cells that express putative progenitor cell markers in human cartilage. In contrast, the percentage of SP cells is much lower and within the range of expected stem cell frequency. Thus, markers such as Notch-1, Stro-1 or VCAM-1 may not be useful to identify progenitors in cartilage. Instead, their increased expression in OA cartilage implicates involvement in the abnormal cell activation and differentiation process characteristic of OA

The Star-Forming Dwarf Galaxy Populations of two z ~ 0.4 Clusters: MS1512.4+3647 and Abell 851

We present the results of a deep narrow-band [OII] 3727 \AA emission-line search for faint ($g <$ 27), star-forming galaxies in the field of the $z=0.37$ MS1512.4+3647 cluster. We find no evidence for an over-density of emission-line sources relative to the field at $z \sim$ 0.4 (Hogg et al. 1998), and therefore conclude that the MS1512.4+3647 sample is dominated by field [OII] emission-line galaxies which lie along the $\sim$ 180 Mpc line of sight immediately in front and behind the cluster. This is surprising, given that the previously surveyed $z=0.41$ cluster Abell 851 has 3-4 times the field emission-line galaxy density (Martin et al. 2000). We find that the MS1512.4+3647 sample is deficient in galaxies with intermediate colors (1.0 $< g-i <$ 2.0) and implied star-formation exponential decay timescales $\tau \sim$ 100 Myr - 1 Gyr that dominate the Abell 851 emission-line galaxy population. Instead, the majority of [OII] emission-line galaxies surrounding the MS1512.4+3647 cluster are blue ($g-i \leq 1.0$) and forming stars in bursts with $\tau <$ 100 Myr. In both samples, galaxies with the shortest star-formation timescales are preferentially among the faintest star-forming objects. Their i luminosities are consistent with young stellar populations \sim 10^8 - 10^9 \Msun, although an additional factor of ten in stellar mass could be hiding in underlying old stellar populations. We discuss the implications for the star-formation histories of dwarf galaxies in the field and rich clusters.Comment: 26 pages, including 5 tables and 13 figures; accepted for publication in the Astrophysical Journa

Hyaluronan concentration and size distribution in human knee synovial fluid: variations with age and cartilage degeneration.

BackgroundOne potential mechanism for early superficial cartilage wear in normal joints is alteration of the lubricant content and quality of synovial fluid. The purpose of this study was to determine if the concentration and quality of the lubricant, hyaluronan, in synovial fluid: (1) was similar in left and right knees; (2) exhibited similar age-associated trends, whether collected postmortem or antemortem; and (3) varied with age and grade of joint degeneration.MethodsHuman synovial fluid of donors (23-91 years) without osteoarthritis was analyzed for the concentrations of protein, hyaluronan, and hyaluronan in the molecular weight ranges of 2.5-7 MDa, 1-2.5 MDa, 0.5-1 MDa, and 0.03-0.5 MDa. Similarity of data between left and right knees was assessed by reduced major axis regression, paired t-test, and Bland-Altman analysis. The effect of antemortem versus postmortem collection on biochemical properties was assessed for age-matched samples by unpaired t-test. The relationships between age, joint grade, and each biochemical component were assessed by regression analysis.ResultsJoint grade and the concentrations of protein, hyaluronan, and hyaluronan in the molecular weight ranges of 2.5-7 MDa, 1-2.5 MDa, and 0.5-1 MDa in human synovial fluid showed good agreement between left and right knees and were similar between age-matched patient and cadaver knee joints. There was an age-associated decrease in overall joint grade (-15 %/decade) and concentrations of hyaluronan (-10.5 %/decade), and hyaluronan in the molecular weight ranges of 2.5-7 MDa (-9.4 %/decade), 1-2.5 MDa (-11.3 %/decade), 0.5-1 MDa (-12.5 %/decade), and 0.03-0.5 MDa (-13.0 %/decade). Hyaluronan concentration and quality was more strongly associated with age than with joint grade.ConclusionsThe age-related increase in cartilage wear in non-osteoarthritic joints may be related to the altered hyaluronan content and quality of synovial fluid

Mohawk promotes the maintenance and regeneration of the outer annulus fibrosus of intervertebral discs.

The main pathogenesis of intervertebral disc (IVD) herniation involves disruption of the annulus fibrosus (AF) caused by ageing or excessive mechanical stress and the resulting prolapse of the nucleus pulposus. Owing to the avascular nature of the IVD and lack of understanding the mechanisms that maintain the IVD, current therapies do not lead to tissue regeneration. Here we show that homeobox protein Mohawk (Mkx) is a key transcription factor that regulates AF development, maintenance and regeneration. Mkx is mainly expressed in the outer AF (OAF) of humans and mice. In Mkx(-/-) mice, the OAF displays a deficiency of multiple tendon/ligament-related genes, a smaller OAF collagen fibril diameter and a more rapid progression of IVD degeneration compared with the wild type. Mesenchymal stem cells overexpressing Mkx promote functional AF regeneration in a mouse AF defect model, with abundant collagen fibril formation. Our results indicate a therapeutic strategy for AF regeneration

MicroRNA-125b regulates the expression of aggrecanase-1 (ADAMTS-4) in human osteoarthritic chondrocytes

INTRODUCTION: Increased expression of aggrecanase-1 (ADAMTS-4) has emerged as an important factor in osteoarthritis (OA) and other joint diseases. This study aimed to determine whether the expression of ADAMTS-4 in human chondrocytes is regulated by miRNA. METHODS: MiRNA targets were identified using bioinformatics. Chondrocytes were isolated from knee cartilage and treated with interleukin-1 beta (IL-1β). Gene expression was quantified using TaqMan assays and protein production was determined by immunoblotting. Luciferase reporter assay was used to verify interaction between miRNA and target messenger RNA (mRNA). RESULTS: In silico analysis predicted putative target sequence of miR-125b on ADAMTS-4. MiR-125b was expressed in both normal and OA chondrocytes, with significantly lower expression in OA chondrocytes than in normal chondrocytes. Furthermore, IL-1β-induced upregulation of ADAMTS-4 was suppressed by overexpression of miR-125b in human OA chondrocytes. In the luciferase reporter assay, mutation of the putative miR-125b binding site in the ADAMTS-4 3'UTR abrogated the suppressive effect of miR125. CONCLUSIONS: Our results indicate that miR-125b plays an important role in regulating the expression of ADAMTS-4 in human chondrocytes and this identifies miR-125b as a novel therapeutic target in OA

The Kiloparsec-Scale Kinematics of High-Redshift Star-Forming Galaxies

We present the results of a spectroscopic survey of the kinematic structure of star-forming galaxies at redshift z ~ 2 - 3 using Keck/OSIRIS integral field spectroscopy. Our sample is comprised of 12 galaxies between redshifts z ~ 2.0 and 2.5 and one galaxy at z ~ 3.3 which are well detected in either HAlpha or [O III] emission. These observations were obtained in conjunction with the Keck laser guide star adaptive optics system, with a typical angular resolution after spatial smoothing ~ 0.15" (approximately 1 kpc at the redshift of the target sample). At most five of these 13 galaxies have spatially resolved velocity gradients consistent with rotation while the remaining galaxies have relatively featureless or irregular velocity fields. All of our galaxies show local velocity dispersions ~ 60 - 100 km/s, suggesting that (particularly for those galaxies with featureless velocity fields) rotation about a preferred axis may not be the dominant mechanism of physical support. While some galaxies show evidence for major mergers such evidence is unrelated to the kinematics of individual components (one of our strongest merger candidates also exhibits unambiguous rotational structure), refuting a simple bimodal disk/merger classification scheme. We discuss these data in light of complementary surveys and extant UV-IR spectroscopy and photometry, concluding that the dynamical importance of cold gas may be the primary factor governing the observed kinematics of z ~ 2 galaxies. We conclude by speculating on the importance of mechanisms for accreting low angular-momentum gas and the early formation of quasi-spheroidal systems in the young universe.(abridged)Comment: 34 pages, 13 figures. Revised version accepted for publication in the Astrophysical Journal. Version with full-resolution figures is available at http://www.astro.ucla.edu/~drlaw/Papers/OSIRIS_data2.pd

Ubiquitous outflows in DEEP2 spectra of star-forming galaxies at z=1.4

Galactic winds are a prime suspect for the metal enrichment of the intergalactic medium and may have a strong influence on the chemical evolution of galaxies and the nature of QSO absorption line systems. We use a sample of 1406 galaxy spectra at z~1.4 from the DEEP2 redshift survey to show that blueshifted Mg II 2796, 2803 A absorption is ubiquitous in starforming galaxies at this epoch. This is the first detection of frequent outflowing galactic winds at z~1. The presence and depth of absorption are independent of AGN spectral signatures or galaxy morphology; major mergers are not a prerequisite for driving a galactic wind from massive galaxies. Outflows are found in coadded spectra of galaxies spanning a range of 30x in stellar mass and 10x in star formation rate (SFR), calibrated from K-band and from MIPS IR fluxes. The outflows have column densities of order N_H ~ 10^20 cm^-2 and characteristic velocities of ~ 300-500 km/sec, with absorption seen out to 1000 km/sec in the most massive, highest SFR galaxies. The velocities suggest that the outflowing gas can escape into the IGM and that massive galaxies can produce cosmologically and chemically significant outflows. Both the Mg II equivalent width and the outflow velocity are larger for galaxies of higher stellar mass and SFR, with V_wind ~ SFR^0.3, similar to the scaling in low redshift IR-luminous galaxies. The high frequency of outflows in the star-forming galaxy population at z~1 indicates that galactic winds occur in the progenitors of massive spirals as well as those of ellipticals. The increase of outflow velocity with mass and SFR constrains theoretical models of galaxy evolution that include feedback from galactic winds, and may favor momentum-driven models for the wind physics.Comment: Accepted by ApJ. 25 pages, 17 figures. Revised to add discussions of intervening absorbers and AGN-driven outflows; conclusions unchange